scholarly journals Advances in the Development of Microbial Double-Stranded RNA Production Systems for Application of RNA Interference in Agricultural Pest Control

2021 ◽  
Vol 9 ◽  
Author(s):  
Ruobing Guan ◽  
Dongdong Chu ◽  
Xinyi Han ◽  
Xuexia Miao ◽  
Haichao Li
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Rna Interference ◽  
Chemical Synthesis ◽  
Future Development ◽  
Production Systems ◽  
Low Cost ◽  
Agricultural Pest ◽  
Double Stranded Rna ◽  
System Selection

RNA interference (RNAi) is a valuable and revolutionary technology that has been widely applied in medicine and agriculture. The application of RNAi in various industries requires large amounts of low-cost double-stranded RNA (dsRNA). Chemical synthesis can only produce short dsRNAs; long dsRNAs need to be synthesized biologically. Several microbial chassis cells, such as Escherichia coli, Saccharomyces cerevisiae, and Bacillus species, have been used for dsRNA synthesis. However, the titer, rate of production, and yield of dsRNA obtained by these microorganism-based strategies is still low. In this review, we summarize advances in microbial dsRNA production, and analyze the merits and faults of different microbial dsRNA production systems. This review provides a guide for dsRNA production system selection. Future development of efficient microbial dsRNA production systems is also discussed.

Microbe Engineering of Guaia-6,10(14)-diene as a Building Block for the Semisynthetic Production of Plant-Derived (−)-Englerin A

2019 ◽  
Author(s):  
Thomas Siemon ◽  
Zhangqian Wang ◽  
Guangkai Bian ◽  
Tobias Seitz ◽  
Ziling Ye ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Synthetic Biology ◽  
Chemical Synthesis ◽  
Building Block ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Economical Method ◽  
Englerin A ◽  
Transient Receptor

Herein, we report the semisynthetic production of the potent transient receptor potential canonical (TRPC) channel agonist (−)-englerin A (EA), using guaia-6,10(14)-diene as the starting material. Guaia-6,10(14)-diene was systematically engineered in Escherichia coli and Saccharomyces cerevisiae using the CRISPR/Cas9 system and produced with high titers. This provided us the opportunity to execute a concise chemical synthesis of EA and the two related guaianes (−)-oxyphyllol and (+)-orientalol E. The potentially scalable approach combines the advantages of synthetic biology and chemical synthesis and provides an efficient and economical method for producing EA as well as its analogs.

Microbe Engineering of Guaia-6,10(14)-diene as a Building Block for the Semisynthetic Production of Plant-Derived (−)-Englerin A

2019 ◽  
Author(s):  
Thomas Siemon ◽  
Zhangqian Wang ◽  
Guangkai Bian ◽  
Tobias Seitz ◽  
Ziling Ye ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Synthetic Biology ◽  
Chemical Synthesis ◽  
Building Block ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Economical Method ◽  
Englerin A ◽  
Transient Receptor

Herein, we report the semisynthetic production of the potent transient receptor potential canonical (TRPC) channel agonist (−)-englerin A (EA), using guaia-6,10(14)-diene as the starting material. Guaia-6,10(14)-diene was systematically engineered in Escherichia coli and Saccharomyces cerevisiae using the CRISPR/Cas9 system and produced with high titers. This provided us the opportunity to execute a concise chemical synthesis of EA and the two related guaianes (−)-oxyphyllol and (+)-orientalol E. The potentially scalable approach combines the advantages of synthetic biology and chemical synthesis and provides an efficient and economical method for producing EA as well as its analogs.

Causes of gear pump fails and methods for their elimination

2020 ◽  
pp. 98-107
Author(s):  
Vitaliy A. Zuyevskiy ◽  
Daniil O. Klimyuk ◽  
Ivan A. Shemberev
Keyword(s):  
Adhesion Strength ◽  
Production Systems ◽  
Low Cost ◽  
High Strength ◽  
Strength Properties ◽  
Research Purpose ◽  
Working Fluid ◽  
Gear Pump ◽  
Repair Service ◽  
Recovery Method

Gear pumps are an important element of many production systems and their replacement in case of failure can be quite expensive, so it is important to have a modern and well-tuned technology for their recovery. There are many methods for restoring the pump's performance, depending on the reason that led to its failure. (Research purpose) The research purpose is in determining what causes most often lead to loss of pump performance, and developing a recovery method that provides the greatest post-repair service life of the pump and low cost of repair. (Materials and methods) Authors took into account that the applied coatings must have sufficient adhesion strength and resistance to mechanical, thermal and corrosion loads during operation. It was found that most often significant leaks of the working fluid, leading to failure, occur due to an increase in the gap between the inner surface of the housing and the gears due to active wear of the housing wells. Authors determined that the method of electric spark treatment of worn-out housing wells is best suited to perform the task (a large post-repair resource and low costs). (Results and discussion) It was found by laboratory studies of the adhesion strength of electric spark coatings with various electrodes that the best transfer of the material to the substrate is provided by bronze electrodes BrMKts3-1. It was noted that the coatings applied using the BrMKts3-1 electrode have high strength properties. (Conclusions) Research conducted in the center for collective use "Nano-Center" VIM confirmed the possibility of effective recovery of the gear pump by electric spark treatment.

scholarly journals Effects of Plant Age and Root Damage on Internalization of Shiga Toxin-Producing Escherichia coli in Leafy Vegetables and Herbs

Horticulturae ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 68
Author(s):  
Yi-Ju Wang ◽  
Amanda J. Deering ◽  
Hye-Ji Kim
Keyword(s):  
Escherichia Coli ◽  
Plant Species ◽  
Shiga Toxin ◽  
Production Systems ◽  
Plant Age ◽  
Leafy Vegetables ◽  
Root Damage ◽  
Hygiene Practices ◽  
Hydroponic Systems ◽  
Stx1 Gene

Our previous study reported that fresh produce grown in aquaponic and hydroponic systems can pose potential food safety hazards due to an accidental introduction of contaminated fish and cross-contamination between the systems. In this study, we examined the effects of plant species and age on the likelihood and level of internalization of Shiga toxin-producing Escherichia coli (STEC) in aquaponic and hydroponic systems. Four plant species, basil (Ocimum basilicum L. cv. Genovese), cilantro (Coriandrum Sativum L.), lettuce (Lactuca sativa cv. Cherokee), and kale (Brassica oleracea var. sabellica), received root damage treatment as seedlings before transplanting or mature plants at three weeks after transplanting by cutting off 1-cm tips of one-third of the roots. Enrichments and selective media were used for the isolation, and presumptive positive colonies were confirmed by PCR for the presence of stx1 gene in plant tissues, recirculating water, and fish feces collected at four weeks after transplanting. In hydroponic systems, STEC was found neither in the solution nor in the roots and leaves of all four plant species, possibly through improved sanitation and hygiene practices. However, consistent with our previous findings, STEC was found in the water, on the plant roots, and in the fish feces in aquaponic systems, even after thorough sanitation prior to the study. Regardless of plant age, STEC was internalized in the roots of all plant species when the roots were damaged, but there was no difference in the degree of internalization with STEC among plant species. STEC was present in the leaves only when seedlings received root damage treatment and were grown to maturity, indicating that root damage allows STEC to internalize in the roots within a week, but a longer period is required for STEC to internalize into the leaves. We concluded that root damage on seedlings can cause the internalization of E. coli O157:H7 in the edible parts of leafy vegetables and herbs in soilless production systems.

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