scholarly journals Polyelectrolyte Multilayer Capsule (PEMC)-Based Scaffolds for Tissue Engineering

Micromachines ◽  
2020 ◽  
Vol 11 (9) ◽  
pp. 797
Author(s):  
Georgia Kastania ◽  
Jack Campbell ◽  
Jacob Mitford ◽  
Dmitry Volodkin
Keyword(s):  
Tissue Engineering ◽  
Drug Testing ◽  
Tissue Growth ◽  
Future Perspectives ◽  
Polyelectrolyte Multilayer ◽  
Advantages And Disadvantages ◽  
Cellular Behaviour ◽  
Straightforward Approach

Tissue engineering (TE) is a highly multidisciplinary field that focuses on novel regenerative treatments and seeks to tackle problems relating to tissue growth both in vitro and in vivo. These issues currently involve the replacement and regeneration of defective tissues, as well as drug testing and other related bioapplications. The key approach in TE is to employ artificial structures (scaffolds) to support tissue development; these constructs should be capable of hosting, protecting and releasing bioactives that guide cellular behaviour. A straightforward approach to integrating bioactives into the scaffolds is discussed utilising polyelectrolyte multilayer capsules (PEMCs). Herein, this review illustrates the recent progress in the use of CaCO3 vaterite-templated PEMCs for the fabrication of functional scaffolds for TE applications, including bone TE as one of the main targets of PEMCs. Approaches for PEMC integration into scaffolds is addressed, taking into account the formulation, advantages, and disadvantages of such PEMCs, together with future perspectives of such architectures.

Bioprinted Nanoparticles for Tissue Engineering Applications

2009 ◽  
Author(s):  
Kivilcim Buyukhatipoglu ◽  
Robert Chang ◽  
Wei Sun ◽  
Alisa Morss Clyne
Keyword(s):  
Tissue Engineering ◽  
Tissue Growth ◽  
Bioactive Components ◽  
Engineering Applications ◽  
Tissue Properties ◽  
Native Tissue ◽  
3D Architecture

Tissue engineering may require precise patterning of cells and bioactive components to recreate the complex, 3D architecture of native tissue. However, it is difficult to image and track cells and bioactive factors once they are incorporated into the tissue engineered construct. These bioactive factors and cells may also need to be moved during tissue growth in vitro or after implantation in vivo to achieve the desired tissue properties, or they may need to be removed entirely prior to implantation for biosafety concerns.

scholarly journals Advanced Hydrogels for Cartilage Tissue Engineering: Recent Progress and Future Directions

Polymers ◽  
2021 ◽  
Vol 13 (23) ◽  
pp. 4199
Author(s):  
Mahshid Hafezi ◽  
Saied Nouri Khorasani ◽  
Mohadeseh Zare ◽  
Rasoul Esmaeely Neisiany ◽  
Pooya Davoodi
Keyword(s):  
Tissue Engineering ◽  
Cartilage Tissue Engineering ◽  
Cartilage Regeneration ◽  
Biomechanical Properties ◽  
Tissue Growth ◽  
Cartilage Tissue ◽  
Self Healing ◽  
Advantages And Disadvantages ◽  
Wide Range

Cartilage is a tension- and load-bearing tissue and has a limited capacity for intrinsic self-healing. While microfracture and arthroplasty are the conventional methods for cartilage repair, these methods are unable to completely heal the damaged tissue. The need to overcome the restrictions of these therapies for cartilage regeneration has expanded the field of cartilage tissue engineering (CTE), in which novel engineering and biological approaches are introduced to accelerate the development of new biomimetic cartilage to replace the injured tissue. Until now, a wide range of hydrogels and cell sources have been employed for CTE to either recapitulate microenvironmental cues during a new tissue growth or to compel the recovery of cartilaginous structures via manipulating biochemical and biomechanical properties of the original tissue. Towards modifying current cartilage treatments, advanced hydrogels have been designed and synthesized in recent years to improve network crosslinking and self-recovery of implanted scaffolds after damage in vivo. This review focused on the recent advances in CTE, especially self-healing hydrogels. The article firstly presents the cartilage tissue, its defects, and treatments. Subsequently, introduces CTE and summarizes the polymeric hydrogels and their advances. Furthermore, characterizations, the advantages, and disadvantages of advanced hydrogels such as multi-materials, IPNs, nanomaterials, and supramolecular are discussed. Afterward, the self-healing hydrogels in CTE, mechanisms, and the physical and chemical methods for the synthesis of such hydrogels for improving the reformation of CTE are introduced. The article then briefly describes the fabrication methods in CTE. Finally, this review presents a conclusion of prevalent challenges and future outlooks for self-healing hydrogels in CTE applications.

scholarly journals Hydrogels for Liver Tissue Engineering

2019 ◽  
Vol 6 (3) ◽  
pp. 59 ◽  
Author(s):  
Shicheng Ye ◽  
Jochem W.B. Boeter ◽  
Louis C. Penning ◽  
Bart Spee ◽  
Kerstin Schneeberger
Keyword(s):  
Tissue Engineering ◽  
Liver Tissue ◽  
Drug Testing ◽  
In Vitro Models ◽  
Liver Tissue Engineering ◽  
Synthetic Materials ◽  
Toxicological Studies ◽  
End Stage

Bioengineered livers are promising in vitro models for drug testing, toxicological studies, and as disease models, and might in the future be an alternative for donor organs to treat end-stage liver diseases. Liver tissue engineering (LTE) aims to construct liver models that are physiologically relevant. To make bioengineered livers, the two most important ingredients are hepatic cells and supportive materials such as hydrogels. In the past decades, dozens of hydrogels have been developed to act as supportive materials, and some have been used for in vitro models and formed functional liver constructs. However, currently none of the used hydrogels are suitable for in vivo transplantation. Here, the histology of the human liver and its relationship with LTE is introduced. After that, significant characteristics of hydrogels are described focusing on LTE. Then, both natural and synthetic materials utilized in hydrogels for LTE are reviewed individually. Finally, a conclusion is drawn on a comparison of the different hydrogels and their characteristics and ideal hydrogels are proposed to promote LTE.

scholarly journals Multimodal/Multifunctional Nanomaterials in (Bio)electrochemistry: Now and in the Coming Decade

Nanomaterials ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 2556
Author(s):  
Paloma Yáñez-Sedeño ◽  
Araceli González-Cortés ◽  
Susana Campuzano ◽  
José Manuel Pingarrón
Keyword(s):  
Tissue Engineering ◽  
Targeted Delivery ◽  
Review Article ◽  
Combined Effect ◽  
Future Perspectives ◽  
Electrochemical Biosensing ◽  
Multifunctional Nanomaterials ◽  
Improved Performance

Multifunctional nanomaterials, defined as those able to achieve a combined effect or more than one function through their multiple functionalization or combination with other materials, are gaining increasing attention in the last years in many relevant fields, including cargo targeted delivery, tissue engineering, in vitro and/or in vivo diseases imaging and therapy, as well as in the development of electrochemical (bio)sensors and (bio)sensing strategies with improved performance. This review article aims to provide an updated overview of the important advances and future opportunities exhibited by electrochemical biosensing in connection to multifunctional nanomaterials. Accordingly, representative aspects of recent approaches involving metal, carbon, and silica-based multifunctional nanomaterials are selected and critically discussed, as they are the most widely used multifunctional nanomaterials imparting unique capabilities in (bio)electroanalysis. A brief overview of the main remaining challenges and future perspectives in the field is also provided.

scholarly journals Scaffold-Based Tissue Engineering Strategies for Osteochondral Repair

2022 ◽  
Vol 9 ◽  
Author(s):  
Jiang-Nan Fu ◽  
Xing Wang ◽  
Meng Yang ◽  
You-Rong Chen ◽  
Ji-Ying Zhang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Metal Composite ◽  
Tissue Engineering Scaffolds ◽  
Advantages And Disadvantages ◽  
Osteochondral Repair ◽  
Cell Growth And Differentiation ◽  
Manufacturing Technologies ◽  
Made In

Over centuries, several advances have been made in osteochondral (OC) tissue engineering to regenerate more biomimetic tissue. As an essential component of tissue engineering, scaffolds provide structural and functional support for cell growth and differentiation. Numerous scaffold types, such as porous, hydrogel, fibrous, microsphere, metal, composite and decellularized matrix, have been reported and evaluated for OC tissue regeneration in vitro and in vivo, with respective advantages and disadvantages. Unfortunately, due to the inherent complexity of organizational structure and the objective limitations of manufacturing technologies and biomaterials, we have not yet achieved stable and satisfactory effects of OC defects repair. In this review, we summarize the complicated gradients of natural OC tissue and then discuss various osteochondral tissue engineering strategies, focusing on scaffold design with abundant cell resources, material types, fabrication techniques and functional properties.

scholarly journals 3D Cell Printing of Tissue/Organ-Mimicking Constructs for Therapeutic and Drug Testing Applications

2020 ◽  
Vol 21 (20) ◽  
pp. 7757
Author(s):  
Jongmin Kim ◽  
Jeong Sik Kong ◽  
Wonil Han ◽  
Byoung Soo Kim ◽  
Dong-Woo Cho
Keyword(s):  
Tissue Engineering ◽  
Drug Testing ◽  
In Vitro Testing ◽  
Living Tissue ◽  
Cell Printing ◽  
Artificial Tissue ◽  
Engineered Tissue ◽  
Pharmaceutical Industries

The development of artificial tissue/organs with the functional maturity of their native equivalents is one of the long-awaited panaceas for the medical and pharmaceutical industries. Advanced 3D cell-printing technology and various functional bioinks are promising technologies in the field of tissue engineering that have enabled the fabrication of complex 3D living tissue/organs. Various requirements for these tissues, including a complex and large-volume structure, tissue-specific microenvironments, and functional vasculatures, have been addressed to develop engineered tissue/organs with native relevance. Functional tissue/organ constructs have been developed that satisfy such criteria and may facilitate both in vivo replenishment of damaged tissue and the development of reliable in vitro testing platforms for drug development. This review describes key developments in technologies and materials for engineering 3D cell-printed constructs for therapeutic and drug testing applications.

scholarly journals 3D Bioprinting of Vascularized Tissues for in vitro and in vivo Applications

2021 ◽  
Vol 9 ◽  
Author(s):  
Earnest P. Chen ◽  
Zeren Toksoy ◽  
Bruce A. Davis ◽  
John P. Geibel
Keyword(s):  
Tissue Engineering ◽  
Drug Testing ◽  
Rapid Development ◽  
Three Dimensional ◽  
3D Bioprinting ◽  
Vascular Networks ◽  
Main Challenge ◽  
3D Printed

With a limited supply of organ donors and available organs for transplantation, the aim of tissue engineering with three-dimensional (3D) bioprinting technology is to construct fully functional and viable tissue and organ replacements for various clinical applications. 3D bioprinting allows for the customization of complex tissue architecture with numerous combinations of materials and printing methods to build different tissue types, and eventually fully functional replacement organs. The main challenge of maintaining 3D printed tissue viability is the inclusion of complex vascular networks for nutrient transport and waste disposal. Rapid development and discoveries in recent years have taken huge strides toward perfecting the incorporation of vascular networks in 3D printed tissue and organs. In this review, we will discuss the latest advancements in fabricating vascularized tissue and organs including novel strategies and materials, and their applications. Our discussion will begin with the exploration of printing vasculature, progress through the current statuses of bioprinting tissue/organoids from bone to muscles to organs, and conclude with relevant applications for in vitro models and drug testing. We will also explore and discuss the current limitations of vascularized tissue engineering and some of the promising future directions this technology may bring.

scholarly journals Cell Microenvironment Engineering and Monitoring for Tissue Engineering and Regenerative Medicine: The Recent Advances

2014 ◽  
Vol 2014 ◽  
pp. 1-18 ◽  
Author(s):  
Julien Barthes ◽  
Hayriye Özçelik ◽  
Mathilde Hindié ◽  
Albana Ndreu-Halili ◽  
Anwarul Hasan ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Regenerative Medicine ◽  
Control Cell ◽  
Matrix Composition ◽  
Tissue Engineering Scaffolds ◽  
Cell Microenvironment ◽  
Engineered Tissues ◽  
Cellular Behaviour

In tissue engineering and regenerative medicine, the conditions in the immediate vicinity of the cells have a direct effect on cells’ behaviour and subsequently on clinical outcomes. Physical, chemical, and biological control of cell microenvironment are of crucial importance for the ability to direct and control cell behaviour in 3-dimensional tissue engineering scaffolds spatially and temporally. In this review, we will focus on the different aspects of cell microenvironment such as surface micro-, nanotopography, extracellular matrix composition and distribution, controlled release of soluble factors, and mechanical stress/strain conditions and how these aspects and their interactions can be used to achieve a higher degree of control over cellular activities. The effect of these parameters on the cellular behaviour within tissue engineering context is discussed and how these parameters are used to develop engineered tissues is elaborated. Also, recent techniques developed for the monitoring of the cell microenvironmentin vitroandin vivoare reviewed, together with recent tissue engineering applications where the control of cell microenvironment has been exploited. Cell microenvironment engineering and monitoring are crucial parts of tissue engineering efforts and systems which utilize different components of the cell microenvironment simultaneously can provide more functional engineered tissues in the near future.

scholarly journals Tubular electrospun scaffolds tested in vivo for tissue engineering

2019 ◽  
Vol 7 (2) ◽  
pp. 635
Author(s):  
Alan Isaac Valderrama-Treviño ◽  
Karen Uriarte-Ruiz ◽  
Juan José Granados Romero ◽  
Andrés Eliú Castell Rodriguez ◽  
Alfredo Maciel-Cerda ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Small Diameter ◽  
Cellular Adhesion ◽  
In Vivo Evaluation ◽  
Structural Support ◽  
Electrospun Scaffolds ◽  
Advantages And Disadvantages ◽  
The Creation

Tissue engineering has been widely used for its great variety of functions. It has been seen as a solution to satisfy the need for vascular substitutes like small diameter vessels, veins, and nerves. One of the most used methods is electrospinning, due to the fact that it allows the use of various polymers, sizes, mandrels and it can adjust the conditions to create personalized scaffolds. For the creation of scaffolds is fundamental to understand the advantages and disadvantages of each polymer, of this, will depend the biodegradability, biocompatibility, porosity, cellular adhesion, and cell proliferation as it is essential to mimic the extracellular matrix and provide structural support for the cells. The aim of this review was to investigate which materials are being used for the creation of tubular scaffolds by electrospinning. Here we selected only in vivo evaluation to demonstrate remodeling of the grafts into native-like tissues, in vitro evaluations had been excluded from this review. We analyze the conditions like speed, distance and voltage and the modifications like growth factors and combinations of natural and synthetic polymers that allow the authors to have a functional scaffold that will suit its purpose.

Eggshell Membrane Protein Modified Silk Fibroin-Poly Vinyl Alcohol Scaffold for Bone Tissue Engineering: In Vitro and In Vivo Study

2017 ◽  
Vol 32 ◽  
pp. 69-81 ◽  
Author(s):  
Mahesh Kumar Sah ◽  
Indranil Banerjee ◽  
Krishna Pramanik
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Silk Fibroin ◽  
Tissue Growth ◽  
Eggshell Membrane ◽  
Poly Vinyl Alcohol ◽  
Porous Network

There is a need for high performance scaffold in tissue engineering. Keeping this perspective in mind, the present study delineates the preparation and physico-chemical characterization of soluble eggshell protein (SEP) modified silk fibroin (SF)-polyvinyl alcohol (PVA) scaffold and its application in bone tissue engineering. The SF/PVA scaffold were prepared by salt leaching and modified with eggshell protein. Micro-architechture and porosity analysis revealed that all the scaffolds were having desired pore size (230-360 µm), interconnected porous network and 90% porosity. The scaffolds were found with suitable swelling behavior and biodegradability to support cell proliferation till replaces native osseous tissue. In vitro cyto-compatibility and differentiation study showed that SEP(SF-PVA) supports viability , proliferation and differentiation of cord blood derived human mesenchymal stem cell. Further, in vivo study in mice model showed that the scaffolds are non-immunogenic and support tissue growth. In conclusion, SEP modified SF-PVA scaffold could be a better option for tissue engineering.

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