scholarly journals Characterization of N6-Methyladenosine in Domesticated Yak Testes Before and After Sexual Maturity

2021 ◽  
Vol 9 ◽  
Author(s):  
Xingdong Wang ◽  
Jie Pei ◽  
Shaoke Guo ◽  
Mengli Cao ◽  
Pengjia Bao ◽  
...  
Keyword(s):  
Sexual Maturation ◽  
Testicular Tissue ◽  
Notch Signaling Pathway ◽  
Regulatory Role ◽  
Testicular Development ◽  
Hormone Synthesis ◽  
Before And After ◽  
Rna Immunoprecipitation

The potential regulatory role of N6-methyladenosine (m6A), the most prominent mRNA modification in eukaryotes, has recently been identified in mammals, plants, and yeast. However, whether and how m6A methylation is involved in sexual maturation in mammals remains largely unexplored. In this study, testicular tissue was obtained from yaks before and after sexual maturation, and m6A maps were generated via preliminary experiments and methylated RNA immunoprecipitation sequencing. Only spermatogonial cells and a few primary spermatocytes were observed in the testicular tissue of yaks before sexual maturation, while spermatogenic cells at different stages of maturity could observed after sexual maturation. Experiments examining the expression of methylation-related enzymes and overall methylation levels showed that the methylation levels in yak testes increased after sexual maturation. Overall, 1,438 methylation peaks were differentially expressed before and after sexual maturation; 1,226 showed significant up-regulation and 212 showed significant down-regulation after sexual maturation. Annotation analysis showed that the differential methylation peaks were most commonly concentrated in the exon region, followed by the 3′UTR and finally the 5′UTR region. KEGG pathway analysis demonstrated that homologous recombination, the Notch signaling pathway, growth hormone synthesis, and other signaling pathways may be involved in testicular development and maturation in yaks. Levels of most m6A modifications were positively correlated with mRNA abundance, suggesting that m6A plays a regulatory role in mammalian sexual maturation. To our knowledge, this is the first report of an m6A transcriptional map of the yak testes, and our study lays the foundation for elucidating the function of m6A in the development of yak testes.

Exploring the Regulatory Role of Wood Vinegar on Heavy Metals (Copper, Zinc) Before and After Cow Dung Composting

2014 ◽  
Vol 13 (15) ◽  
pp. 2453-2460
Author(s):  
Zhiyu Li ◽  
Ling Zhou ◽  
Fei Liu ◽  
Xuening Luo ◽  
Jinlong Sun ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Regulatory Role ◽  
Cow Dung ◽  
Wood Vinegar ◽  
Before And After ◽  
Copper Zinc

scholarly journals Estrogenic induction of spermatogenesis in the hypogonadal (hpg) mouse: role of androgens

Reproduction ◽  
2005 ◽  
Vol 130 (5) ◽  
pp. 643-654 ◽  
Author(s):  
Helen Baines ◽  
Margaret O Nwagwu ◽  
Edwina C Furneaux ◽  
Jane Stewart ◽  
Jeffrey B Kerr ◽  
...  
Keyword(s):  
Seminal Vesicles ◽  
Testicular Development ◽  
Direct Effects ◽  
Estradiol Treatment ◽  
Hormone Synthesis ◽  
Androgen Receptor Antagonist ◽  
Congenital Deficiency ◽  
Testicular Weight ◽  
Normal Spermatogenesis

Testicular development is arrested in the hypogonadal (hpg) mouse due to a congenital deficiency of hypothalamic gonadotropin-releasing hormone synthesis. Previous studies have demonstrated that chronic treatment of these mice with estradiol induces testicular maturation and qualitatively normal spermatogenesis, but it is not known whether these are direct effects via estrogen receptors expressed in the testis, or indirect actions via the pituitary gland. The aim of the current studies was to determine whether the actions of estradiol require the presence of androgens. Sensitive assays revealed that chronic estradiol treatment produced time-dependent increases in pituitary FSH production but no increases in pituitary LH or testicular testosterone content could be detected. As a functional test of androgen dependence, hpg mice were treated for 70 days with estradiol plus Casodex (bicalutamide), an androgen receptor antagonist. Casodex treatment markedly attenuated both the estradiol-induced increase in testicular weight and the proliferation of the seminiferous epithelium, as revealed by morphometric analysis. However, it did not affect the estradiol-induced increase in pituitary FSH content, nor did it affect estradiol-induced increases in the weight of the seminal vesicles and epididymides. We conclude that increased FSH production is not sufficient to explain the increase in testicular development induced by estradiol in hpg mice; there is a requirement for functional androgen receptors for induction of testicular growth.

scholarly journals The Transcriptome-Wide N6-Methyladenosine (m6A) Map Profiling Reveals the Regulatory Role of m6A in the Yak Ovary

2021 ◽  
Author(s):  
Shaoke Guo ◽  
Xingdong Wang ◽  
Mengli Cao ◽  
Xiaoyun Wu ◽  
Lin Xiong ◽  
...  
Keyword(s):  
Untranslated Region ◽  
Follicular Development ◽  
Distribution Patterns ◽  
Regulatory Role ◽  
Ovarian Activity ◽  
Conserved Sequence ◽  
Rna Immunoprecipitation ◽  
Solid Foundation ◽  
Regulation Of Synthesis

Abstract Background and aim: Yak estrus is a seasonal phenomenon, probably involving epigenetic regulation of synthesis and secretion of sex hormones as well as growth and development of follicles. N6-methyladenosine (m6A) is the most common internal modification of the eukaryotic mRNA. However, there are no detailed reports on the m6A transcriptome map of yak ovary. Therefore, this study aimed to collected the yak ovarian tissues at three different states of anestrus (YO-A), estrus (YO-F), and pregnancy (YO-P), and obtained the full transcriptome m6A map in yak by MeRIP-seq. Results: The HE staining revealed that the number of growing follicles and mature follicles in the ovary during the estrus period was relatively higher than those in the estrus period and the pregnancy period. The RT-qPCR showed that the expression of METTL3, METTL14, FTO, YTHDC1 were significantly different across different periods in the ovaries, which suggests that m6A may play a regulatory role in ovarian activity. Next, we identified 20174, 19747 and 13523 m6A peaks in the three ovarian samples of YO-A, YO-F and YO-P using the methylated RNA immunoprecipitation sequencing (MeRIP-seq). The m6A peaks are highly enriched in the coding sequence (CDS) region and 3′untranslated region (3′UTR) as well as the conserved sequence of “RRACH.” Functional analysis revealed the involvement of m6A in many physiological activities of the yak’s ovary during reproductive cycle. The association analysis found that some genes such as BNC1, HOMER1, BMP15, BMP6, GPX3, and WNT11 were related to ovarian functions. Conclusions: The comparison of the distribution patterns of methylation peaks in the ovarian tissues across different periods further explored the m6A markers related to the regulation of ovarian ovulation and follicular development in the yak ovary. This comprehensive map provides a solid foundation for revealing the potential function of the mRNA m6A modification in the yak ovary.

scholarly journals Chemosensory Sensitivity after Coffee Consumption Is Not Static: Short-Term Effects on Gustatory and Olfactory Sensitivity

Foods ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 493
Author(s):  
Alexander W. Fjaeldstad ◽  
Henrique M. Fernandes
Keyword(s):  
Coffee Consumption ◽  
Olfactory Sensitivity ◽  
Short Term ◽  
Coffee Intake ◽  
Testing Procedures ◽  
Before And After ◽  
Decaffeinated Coffee ◽  
Chemosensory Function

Chemosensory sensitivity has great variation between individuals. This variation complicates the chemosensory diagnostics, as well as the creation of a meal with universally high hedonic value. To ensure accurate characterization of chemosensory function, a common rule of thumb is to avoid food/beverages one hour before chemosensory testing. However, the scientific foundation of this time of fast remains unclear. Furthermore, the role of coffee on immediate chemosensitivity is not known and may have implications for optimization of gastronomy and hedonia. The aim of this study is to investigate the modularity effects of coffee consumption on immediate gustatory and olfactory sensitivity. We included 155 participants. By applying tests for olfactory and gustatory sensitivity before and after coffee intake, we found no changes in olfactory sensitivity, but significantly altered sensitivity for some basic tastants. We repeated our experimental paradigm using decaffeinated coffee and found similar results. Our results demonstrate that coffee (regular and decaffeinated) alters the subsequent perception of taste, specifically by increasing the sensitivity to sweet and decreasing the sensitivity to bitter. Our findings provide the first evidence of how coffee impacts short-term taste sensitivity and consequently the way we sense and perceive food following coffee intake—an important insight in the context of gastronomy, as well as in chemosensory testing procedures.

Sign in / Sign up

Export Citation Format

Share Document