Genetic characterization of a flap1 null mutation in Arabidopsis npq4 and pgr5 plants suggests that the regulatory role of FLAP1 involves the control of proton homeostasis in chloroplasts

The INDETERMINATE DOMAIN (IDD) genes comprise a conserved transcription factor family that regulates a variety of developmental and physiological processes in plants. Many recent studies have focused on the genetic characterization of IDD family members and revealed various biological functions, including modulation of sugar metabolism and floral transition, cold stress response, seed development, plant architecture, regulation of hormone signaling, and ammonium metabolism. In this review, we summarize the functions and working mechanisms of the IDD gene family in the regulatory network of metabolism and developmental processes.

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Characterization of DNA demethylation in normal and cancerous cell lines and the regulatory role of cell cycle proteins in human DNA demethylase activity

Journal of Cellular Biochemistry ◽

10.1002/jcb.10749 ◽

2004 ◽

Vol 91 (3) ◽

pp. 572-583 ◽

Cited By ~ 24

Author(s):

Mariappan Vairapandi

Keyword(s):

Cell Cycle ◽

Cell Lines ◽

Dna Demethylation ◽

Regulatory Role ◽

Cell Cycle Proteins ◽

Human Dna ◽

Cancerous Cell ◽

Demethylase Activity

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Structural and Genetic Characterization of Glycosylation of Type a Flagellin in Pseudomonas aeruginosa

Journal of Bacteriology ◽

10.1128/jb.186.9.2523-2531.2004 ◽

2004 ◽

Vol 186 (9) ◽

pp. 2523-2531 ◽

Cited By ~ 92

Author(s):

M. Schirm ◽

S. K. Arora ◽

A. Verma ◽

E. Vinogradov ◽

P. Thibault ◽

...

Keyword(s):

Pseudomonas Aeruginosa ◽

Gene Cluster ◽

Genetic Characterization ◽

Type A ◽

Protein Backbone ◽

Central Surface

ABSTRACT Type a flagellins from two strains of Pseudomonas aeruginosa, strains PAK and JJ692, were found to be glycosylated with unique glycan structures. In both cases, two sites of O-linked glycosylation were identified on each monomer, and these sites were localized to the central, surface-exposed domain of the monomer in the assembled filament. The PAK flagellin was modified with a heterogeneous glycan comprising up to 11 monosaccharide units that were O linked through a rhamnose residue to the protein backbone. The flagellin of JJ692 was less complex and had a single rhamnose substitution at each site. The role of the glycosylation island gene cluster in the production of each of these glycosyl moieties was investigated. These studies revealed that the orfA and orfN genes were required for attachment of the heterologous glycan and the proximal rhamnose residue, respectively.

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Antigenic and genetic characterization of rabies viruses isolated from domestic and wild animals of Brazil identifies the hoary fox as a rabies reservoir

Journal of General Virology ◽

10.1099/vir.0.81223-0 ◽

2005 ◽

Vol 86 (11) ◽

pp. 3153-3162 ◽

Cited By ~ 28

Author(s):

F. Bernardi ◽

S. A. Nadin-Davis ◽

A. I. Wandeler ◽

J. Armstrong ◽

A. A. B. Gomes ◽

...

Keyword(s):

Animal Species ◽

Genetic Characterization ◽

Variable Region ◽

Epidemiological Studies ◽

Genetic Classification ◽

Livestock Species ◽

Central Variable Region ◽

Vampire Bats

Fifty Brazilian rabies viruses, collected from many different animal species and several regions of the country, were characterized by partial sequencing of the central, variable region of the P gene, a locus useful for sensitive molecular epidemiological studies. Phylogenetic analysis of the sequences, which included comparison with other rabies strains recovered from throughout the Americas, identified three main groups of Brazilian viruses, arbitrarily designated BRL-1 to BRL-3. BRL-1 was found in terrestrial carnivores and clusters with other American strains of the cosmopolitan lineage. BRL-2 comprised two distinct isolates, recovered from two species of non-haematophagous bats, that had evolutionary links to insectivorous-bat-derived strains of North America. BRL-3 consisted of isolates from vampire bats and from livestock species probably infected via contact with vampire bats. The terrestrial group was further subdivided into three subtypes: BRL-1a was associated exclusively with dogs and cats, while BRL-1b and BRL-1c were found exclusively in hoary foxes. These observations strongly support the role of the Brazilian hoary fox as a rabies reservoir. Screening of representative Brazilian rabies viruses against a collection of anti-rabies monoclonal antibodies (mAbs) identified a small panel of mAbs that could be used to discriminate between all Brazilian subgroups as defined by genetic classification in this study.

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Plasmodium matutinum Causing Avian Malaria in Lovebirds (Agapornis roseicollis) Hosted in an Italian Zoo

Microorganisms ◽

10.3390/microorganisms9071356 ◽

2021 ◽

Vol 9 (7) ◽

pp. 1356

Author(s):

Cristiano Cocumelli ◽

Manuela Iurescia ◽

Elena Lavinia Diaconu ◽

Valentina Galietta ◽

Caterina Raso ◽

...

Keyword(s):

Cause Of Death ◽

Avian Malaria ◽

Genetic Characterization ◽

Plasmodium Species ◽

Clinical History ◽

Spheniscus Demersus ◽

Gross Lesions ◽

Possible Cause

Avian malaria is a worldwide distributed, vector-born disease of birds caused by parasites of the order Haemosporida. There is a lack of knowledge about the presence and pathogenetic role of Haemosporida in Psittacidae. Here we report a case of avian malaria infection in lovebirds (Agapornis roseicollis), with the genetic characterization of the Plasmodium species involved. The birds were hosted in a zoo located in Italy, where avian malaria cases in African penguins (Spheniscus demersus) were already reported. Animals (n = 11) were submitted for necropsy after sudden death and were subjected to further analyses including histopathology, bacteriology, and PCR for the research of haemosporidians. Clinical history, gross lesions and histopathological observation of schizonts, together with positive PCR results for Plasmodium spp., demonstrated that avian malaria was the cause of death for one animal and the possible cause of death for the other nine. The sequences obtained were compared using BLAST and analyzed for similarity to sequences available at the MalAvi database. Genetic analyses demonstrated a 100% nucleotide identity to Plasmodium matutinum LINN1 for all the obtained sequences. To our knowledge, this is the first report describing avian malaria in lovebirds.

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