scholarly journals The Advance of CRISPR-Cas9-Based and NIR/CRISPR-Cas9-Based Imaging System

2021 ◽  
Vol 9 ◽  
Author(s):  
Huanhuan Qiao ◽  
Jieting Wu ◽  
Xiaodong Zhang ◽  
Jian Luo ◽  
Hao Wang ◽  
...  
Keyword(s):  
Real Time ◽  
Gene Editing ◽  
Imaging System ◽  
Development Trend ◽  
Imaging Systems ◽  
Advanced Imaging ◽  
Nir Imaging ◽  
Targeting Ability ◽  
The Development Trend

The study of different genes, chromosomes and the spatiotemporal relationship between them is of great significance in the field of biomedicine. CRISPR-Cas9 has become the most widely used gene editing tool due to its excellent targeting ability. In recent years, a series of advanced imaging technologies based on Cas9 have been reported, providing fast and convenient tools for studying the sites location of genome, RNA, and chromatin. At the same time, a variety of CRISPR-Cas9-based imaging systems have been developed, which are widely used in real-time multi-site imaging in vivo. In this review, we summarized the component and mechanism of CRISPR-Cas9 system, overviewed the NIR imaging and the application of NIR fluorophores in the delivery of CRISPR-Cas9, and highlighted advances of the CRISPR-Cas9-based imaging system. In addition, we also discussed the challenges and potential solutions of CRISPR-Cas9-based imaging methods, and looked forward to the development trend of the field.

scholarly journals Performance of a novel protease-activated fluorescent imaging system for intraoperative detection of residual breast cancer during breast conserving surgery

2021 ◽  
Vol 187 (1) ◽  
pp. 145-153
Author(s):  
Conor R. Lanahan ◽  
Bridget N. Kelly ◽  
Michele A. Gadd ◽  
Michelle C. Specht ◽  
Carson L. Brown ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Real Time ◽  
Imaging System ◽  
Ex Vivo ◽  
Menopausal Status ◽  
Fluorescent Imaging ◽  
Cancer Subtypes ◽  
Surgical Workflow ◽  
Tumor Types

Abstract Purpose Safe breast cancer lumpectomies require microscopically clear margins. Real-time margin assessment options are limited, and 20–40% of lumpectomies have positive margins requiring re-excision. The LUM Imaging System previously showed excellent sensitivity and specificity for tumor detection during lumpectomy surgery. We explored its impact on surgical workflow and performance across patient and tumor types. Methods We performed IRB-approved, prospective, non-randomized studies in breast cancer lumpectomy procedures. The LUM Imaging System uses LUM015, a protease-activated fluorescent imaging agent that identifies residual tumor in the surgical cavity walls. Fluorescent cavity images were collected in real-time and analyzed using system software. Results Cavity and specimen images were obtained in 55 patients injected with LUM015 at 0.5 or 1.0 mg/kg and in 5 patients who did not receive LUM015. All tumor types were distinguished from normal tissue, with mean tumor:normal (T:N) signal ratios of 3.81–5.69. T:N ratios were 4.45 in non-dense and 4.00 in dense breasts (p = 0.59) and 3.52 in premenopausal and 4.59 in postmenopausal women (p = 0.19). Histopathology and tumor receptor testing were not affected by LUM015. Falsely positive readings were more likely when tumor was present < 2 mm from the adjacent specimen margin. LUM015 signal was stable in vivo at least 6.5 h post injection, and ex vivo at least 4 h post excision. Conclusions Intraoperative use of the LUM Imaging System detected all breast cancer subtypes with robust performance independent of menopausal status and breast density. There was no significant impact on histopathology or receptor evaluation.

scholarly journals Real-Time In Vivo Bioluminescent Imaging for Evaluating the Efficacy of Antibiotics in a Rat Staphylococcus aureus Endocarditis Model

2005 ◽  
Vol 49 (1) ◽  
pp. 380-387 ◽  
Author(s):  
Yan Q. Xiong ◽  
Julie Willard ◽  
Jagath L. Kadurugamuwa ◽  
Jun Yu ◽  
Kevin P. Francis ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Real Time ◽  
Imaging System ◽  
Bioluminescent Imaging ◽  
Vancomycin Therapy ◽  
Treatment Groups ◽  
Highly Sensitive ◽  
Relevant Animal Model ◽  
And Control

ABSTRACT Therapeutic options for invasive Staphylococcus aureus infections have become limited due to rising antimicrobial resistance, making relevant animal model testing of new candidate agents more crucial than ever. In the present studies, a rat model of aortic infective endocarditis (IE) caused by a bioluminescently engineered, biofilm-positive S. aureus strain was used to evaluate real-time antibiotic efficacy directly. This strain was vancomycin and cefazolin susceptible but gentamicin resistant. Bioluminescence was detected and quantified daily in antibiotic-treated and control animals with IE, using a highly sensitive in vivo imaging system (IVIS). Persistent and increasing cardiac bioluminescent signals (BLS) were observed in untreated animals. Three days of vancomycin therapy caused significant reductions in both cardiac BLS (>10-fold versus control) and S. aureus densities in cardiac vegetations (P < 0.005 versus control). However, 3 days after discontinuation of vancomycin therapy, a greater than threefold increase in cardiac BLS was observed, indicating relapsing IE (which was confirmed by quantitative culture). Cefazolin resulted in modest decreases in cardiac BLS and bacterial densities. These microbiologic and cardiac BLS differences during therapy correlated with a longer time-above-MIC for vancomycin (>12 h) than for cefazolin (∼4 h). Gentamicin caused neither a reduction in cardiac S. aureus densities nor a reduction in BLS. There were significant correlations between cardiac BLS and S. aureus densities in vegetations in all treatment groups. These data suggest that bioluminescent imaging provides a substantial advance in the real-time monitoring of the efficacy of therapy of invasive S. aureus infections in live animals.

Development of Real Time High Energy X-Ray Imaging System for Use in Dynamic Fluoroscopy of Aero Gas Turbines

1975 ◽  
Author(s):  
A. E. Stewart
Keyword(s):  
Real Time ◽  
Gas Turbines ◽  
Imaging System ◽  
High Energy ◽  
Cesium Iodide ◽  
Imaging Systems ◽  
X Ray ◽  
X Ray Imaging ◽  
Different Types

This paper discusses the development of a real-time high energy x-ray imaging system for use in dynamic fluoroscopy of aero gas turbines. In order to cover the range of subjects on gas turbines, over ten combinations of film and screen types are used. Three different types of x-ray imaging systems were considered for use: direct type intensifiers (cesium iodide phosphors), and indirect type intensifiers — Marconi “Marionette” and the Oude Delft “Delcalix.”

Research of Localization Issues in Underwater Sensor Networks

2012 ◽  
Vol 157-158 ◽  
pp. 424-427
Author(s):  
Xian Min Wei
Keyword(s):  
Sensor Networks ◽  
Target Tracking ◽  
Real Time ◽  
Development Trend ◽  
Underwater Sensor Networks ◽  
Localization Problem ◽  
Advantages And Disadvantages ◽  
Localization Algorithms ◽  
Underwater Localization ◽  
The Development Trend

Localization problem has great significance in the applications of underwater sensor networks (UWSN), such as real-time monitoring, target tracking and navigation. This paper analized a series of challenges in underwater localization problem, introduced exsiting localization algorithms and discussed their advantages and disadvantages, and then the development trend of localization problem is discussed. Finally, we give some suggestions in the design of localization algoprithms.

scholarly journals Real-Time Lossless Compression Algorithm for Ultrasound Data Using BL Universal Code

2018 ◽  
Author(s):  
Jung Hoon Kim ◽  
Sunmi Yeo ◽  
Jong Won Kim ◽  
Kyeongsoon Kim ◽  
Tai-kyong Song ◽  
...  
Keyword(s):  
Real Time ◽  
Ultrasound Imaging ◽  
Data Transfer ◽  
Lossless Compression ◽  
Imaging Systems ◽  
Universal Code ◽  
High Data ◽  
High Flexibility

Software-based ultrasound imaging systems provide high flexibility that allows easy and fast adoption of newly developed algorithms. However, the extremely high data rate required for data transfer from sensors (e.g., transducers) to the ultrasound imaging systems is a major bottleneck in the software-based architecture, especially in the context of real-time imaging. To overcome this limitation, in this paper, we present a Binary cLuster (BL) code, which yields an improved compression ratio compared to the exponential Golomb code. Owing to the real-time encoding/decoding features without overheads, the universal code is a good solution to reduce the data transfer rate for software-based ultrasound imaging. The performance of the proposed method was evaluated using in vitro and in vivo data sets. It was demonstrated that the BL-beta code has a good stable lossless compression performance of 20 ~ 30% while requiring no auxiliary memory or storage.

scholarly journals Towards clinical photoacoustic and ultrasound imaging: Probe improvement and real-time graphical user interface

2020 ◽  
Vol 245 (4) ◽  
pp. 321-329 ◽  
Author(s):  
Jeesu Kim ◽  
Eun-Yeong Park ◽  
Byullee Park ◽  
Wonseok Choi ◽  
Ki J Lee ◽  
...  
Keyword(s):  
User Interface ◽  
Real Time ◽  
Ultrasound Imaging ◽  
Graphical User Interface ◽  
Imaging System ◽  
Photoacoustic Imaging ◽  
Clinical Applications ◽  
Imaging Systems ◽  
Imaging Probe ◽  
Conventional Ultrasound

Photoacoustic imaging is a non-invasive and non-ionizing biomedical technique that has been investigated widely for various clinical applications. By taking the advantages of conventional ultrasound imaging, hand-held operation with a linear array transducer should be favorable for successful clinical translation of photoacoustic imaging. In this paper, we present new key updates contributed to the previously developed real-time clinical photoacoustic and ultrasound imaging system for improving the clinical usability of the system. We developed a seamless image optimization platform, designed a real-time parameter control software with a user-friendly graphical user interface, performed Monte Carlo simulation of the optical fluence in the imaging plane, and optimized the geometry of the imaging probe. The updated system allows optimizing of all imaging parameters while continuously acquiring the photoacoustic and ultrasound images in real-time. The updated system has great potential to be used in a variety of clinical applications such as assessing the malignancy of thyroid cancer, breast cancer, and melanoma. Impact statement Photoacoustic imaging is a promising biomedical imaging modality that can visualize both structural and functional information of biological tissue. Because of its easiness to be integrated with conventional ultrasound imaging systems, numerous studies have been conducted to develop and apply clinical photoacoustic imaging systems. However, most of the systems were not suitable for general-purpose clinical applications due to one of the following reasons: target specific design, immobility, inaccessible operation sequence, and lack of hand-held operation. This study demonstrates a real-time clinical photoacoustic and ultrasound imaging system, which can overcome the limitations of the previous systems for successful clinical translation.

Wavefront Coded Imaging Systems for MEMS Analysis

2002 ◽  
Author(s):  
Daniel L. Barton ◽  
Jeremy A. Walraven ◽  
Edward R. Dowski ◽  
Rainer Danz ◽  
Andreas Faulstich ◽  
...  
Keyword(s):  
Signal Processing ◽  
Real Time ◽  
High Performance ◽  
Imaging System ◽  
Three Dimensional ◽  
Imaging Systems ◽  
Depth Of Field ◽  
Real Time Imaging ◽  
Large Depth ◽  
Wavefront Coding

Abstract A new imaging technique called Wavefront Coding allows real-time imaging of three-dimensional structures over a very large depth. Wavefront Coding systems combine aspheric optics and signal processing to achieve depth of fields ten or more times greater than that possible with traditional imaging systems. Understanding the relationships between traditional and modern imaging system design through Wavefront Coding is very challenging. In high performance imaging systems nearly all aspects of the system that could reduce image quality are carefully controlled. Modifying the optics and using signal processing can increase the amount of image information that can be recorded by microscopes. For a number of applications this increase in information can allow a single image to be used where a number of images taken at different object planes had been used before. Having very large depth of field and real-time imaging capability means that very deep structures such as surface micromachined MEMS can be clearly imaged with one image, greatly simplifying defect and failure analysis.

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