L-Arginine Enhances Intracellular Killing of Carbapenem-Resistant Klebsiella pneumoniae ST258 by Murine Neutrophils

Urinary tract infections (UTIs) are common in clinics and hospitals and are associated with a high economic burden. Enterobacterium Klebsiella pneumoniae is a prevalent agent causing UTIs. A high prevalence of carbapenem-resistant K. pneumoniae (CRKP) has emerged recently and is continuing to increase. Seventeen urinary CRKP isolates collected at a teaching hospital in Taiwan from December 2016 to September 2017 were analyzed to elucidate their drug resistance mechanisms. Two-thirds of the isolates were obtained from outpatients. Antimicrobial susceptibility tests demonstrated multidrug resistance in all the isolates. Multilocus sequence typing analysis showed high diversity among the isolates. PCR analysis demonstrated the presence of carbapenemases in three isolates. All isolates carried at least one other extended-spectrum β-lactamase, including TEM, DHA, and CTX-M. Fifteen isolates contained mutations in one of the outer membrane porins that were assessed. The expression levels of the acrB and/or oqxB efflux pump genes, as determined by qRT-PCR, were upregulated in 11 isolates. Six isolates might have utilized other efflux pumps or antimicrobial resistance mechanisms. These analyses demonstrated a highly diverse population and the presence of complex resistance mechanisms in urinary isolates of K. pneumoniae.

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Six-year trends of resistance characteristics in carbapenem-resistant Klebsiella pneumoniae isolated from Malaysia

International Journal of Infectious Diseases ◽

10.1016/j.ijid.2020.09.207 ◽

2020 ◽

Vol 101 ◽

pp. 67-68

Author(s):

C.S.J. Teh ◽

Z.X. Kong ◽

M.Y. Lau ◽

F.E. Teng ◽

Y.Q. Lee ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Carbapenem Resistant

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Difference in biofilm formation between carbapenem-resistant and carbapenem-sensitive Klebsiella pneumoniae based on analysis of mrkH distribution

Microbial Pathogenesis ◽

10.1016/j.micpath.2021.104743 ◽

2021 ◽

Vol 152 ◽

pp. 104743

Author(s):

Renchi Fang ◽

Haiyang Liu ◽

Xiucai Zhang ◽

Guofeng Dong ◽

Jiahui Li ◽

...

Keyword(s):

Klebsiella Pneumoniae ◽

Biofilm Formation ◽

Carbapenem Resistant

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A Bibliometric Meta-Analysis of Colistin Resistance in Klebsiella pneumoniae

Diseases ◽

10.3390/diseases9020044 ◽

2021 ◽

Vol 9 (2) ◽

pp. 44

Author(s):

Ozioma Forstinus Nwabor ◽

Pawarisa Terbtothakun ◽

Supayang P. Voravuthikunchai ◽

Sarunyou Chusri

Keyword(s):

Klebsiella Pneumoniae ◽

Research Collaboration ◽

Research Output ◽

Meta Analysis ◽

Research Articles ◽

Colistin Resistance ◽

Carbapenem Resistant ◽

The Usa ◽

Carbapenem Resistant Enterobacteriaceae ◽

Linkage Strength

Colistin is a last resort antibiotic medication for the treatment of infections caused by carbapenem-resistant Klebsiella pneumoniae. In recent years, various mechanisms have been reported to mediate colistin resistance in K. pneumoniae. This study reports a bibliometric analysis of published articles retrieved from the Scopus database relating to colistin resistance in K. pneumoniae. The research trends in colistin resistance and mechanisms of resistance were considered. A total of 1819 research articles published between 1995 and 2019 were retrieved, and the results indicated that 50.19% of the documents were published within 2017–2019. The USA had the highest participation with 340 (14.31%) articles and 14087 (17.61%) citations. Classification based on the WHO global epidemiological regions showed that the European Region contributed 42% of the articles while the American Region contributed 21%. The result further indicated that 45 countries had published at least 10 documents with strong international collaborations amounting to 272 links and a total linkage strength of 735. A total of 2282 keywords were retrieved; however, 57 keywords had ≥15 occurrences with 764 links and a total linkage strength of 2388. Furthermore, mcr-1, colistin resistance, NDM, mgrB, ceftazidime-avibactam, MDR, combination therapy, and carbapenem-resistant Enterobacteriaceae were the trending keywords. Concerning funders, the USA National Institute of Health funded 9.1% of the total research articles, topping the list. The analysis indicated poor research output, collaboration, and funding from Africa and South-East Asia and demands for improvement in international research collaboration.

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Biosynthesis of Zinc Oxide Nanoparticles from Acacia nilotica (L.) Extract to Overcome Carbapenem-Resistant Klebsiella Pneumoniae

Molecules ◽

10.3390/molecules26071919 ◽

2021 ◽

Vol 26 (7) ◽

pp. 1919

Author(s):

Elsayim Rasha ◽

AlOthman Monerah ◽

Alkhulaifi Manal ◽

Ali Rehab ◽

Doud Mohammed ◽

...

Keyword(s):

Electron Microscopy ◽

Zinc Oxide ◽

Klebsiella Pneumoniae ◽

Zinc Oxide Nanoparticles ◽

Acacia Nilotica ◽

Oxide Nanoparticles ◽

Zno Nps ◽

X Ray ◽

Carbapenem Resistant

Recently, concerns have been raised globally about antimicrobial resistance, the prevalence of which has increased significantly. Carbapenem-resistant Klebsiella pneumoniae (KPC) is considered one of the most common resistant bacteria, which has spread to ICUs in Saudi Arabia. This study was established to investigate the antibacterial activity of biosynthesized zinc oxide nanoparticles (ZnO-NPs) against KPC in vitro and in vivo. In this study, we used the aqueous extract of Acacia nilotica (L.) fruits to mediate the synthesis of ZnO-NPs. The nanoparticles produced were characterized by UV-vis spectroscopy, zetasizer and zeta potential analyses, X-ray diffraction (XRD) spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). The antimicrobial activity of ZnO-NPs against KPC was determined via the well diffusion method, and determining minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), the results showed low MIC and MBC when compared with the MIC and MBC of Imipenem and Meropenem antibiotics. The results of in vitro analysis were supported by the results upon applying ZnO-NP ointment to promote wound closure of rats, which showed better wound healing than the results with imipenem ointment. The biosynthesized ZnO-NPs showed good potential for use against bacteria due to their small size, applicability, and low toxicity to human cells.

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