Biosynthesis of Zinc Oxide Nanoparticles from Acacia nilotica (L.) Extract to Overcome Carbapenem-Resistant Klebsiella Pneumoniae

Recently, concerns have been raised globally about antimicrobial resistance, the prevalence of which has increased significantly. Carbapenem-resistant Klebsiella pneumoniae (KPC) is considered one of the most common resistant bacteria, which has spread to ICUs in Saudi Arabia. This study was established to investigate the antibacterial activity of biosynthesized zinc oxide nanoparticles (ZnO-NPs) against KPC in vitro and in vivo. In this study, we used the aqueous extract of Acacia nilotica (L.) fruits to mediate the synthesis of ZnO-NPs. The nanoparticles produced were characterized by UV-vis spectroscopy, zetasizer and zeta potential analyses, X-ray diffraction (XRD) spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM). The antimicrobial activity of ZnO-NPs against KPC was determined via the well diffusion method, and determining minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), the results showed low MIC and MBC when compared with the MIC and MBC of Imipenem and Meropenem antibiotics. The results of in vitro analysis were supported by the results upon applying ZnO-NP ointment to promote wound closure of rats, which showed better wound healing than the results with imipenem ointment. The biosynthesized ZnO-NPs showed good potential for use against bacteria due to their small size, applicability, and low toxicity to human cells.

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Effects of Zinc Oxide Nanoparticles Synthesized Using Aspergillus niger on Carbapenem-Resistant Klebsiella pneumonia In Vitro and In Vivo

Frontiers in Cellular and Infection Microbiology ◽

10.3389/fcimb.2021.748739 ◽

2021 ◽

Vol 11 ◽

Author(s):

Elsayim Rasha ◽

Manal M. Alkhulaifi ◽

Monerah AlOthman ◽

Ibrahim Khalid ◽

Elnagar Doaa ◽

...

Keyword(s):

Zinc Oxide ◽

Aspergillus Niger ◽

Zinc Oxide Nanoparticles ◽

Diffusion Method ◽

Oxide Nanoparticles ◽

Zno Nps ◽

X Ray ◽

Carbapenem Resistant

Currently, the mortality rate in Saudi Arabia’s ICUs is increasing due to the spread of Klebsiella pneumoniae carbapenemase (KPC)-producing bacteria. This study was carried out to evaluate the ability of biologically synthesized zinc oxide nanoparticles (ZnO-NPs) using Aspergillus niger to overcome carbapenem-resistant K. pneumoniae (KPC) in vitro and in vivo. ZnO-NPs were synthesized via a biological method and characterized using UV–Vis spectroscopy, Zetasizer and zeta potential analyses, x-ray diffraction spectroscopy, Fourier transform infrared spectroscopy, scanning electron microscopy (SEM), and energy-dispersive x-ray spectroscopy (EDX). In vitro sensitivity of KPC to ZnO-NPs was identified using the well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined by a macro-dilution method. The morphological alteration of KPC cells after ZnO-NPs treatment was observed by SEM. The in vivo susceptibility of KPC cells to ZnO-NPs ointment was evaluated using wound healing in experimental rats. The chemical characterization findings showed the formation, stability, shape, and size of the synthesized nanoparticles. The MIC and MBC were 0.7 and 1.8 mg/ml, respectively. The in vivo results displayed reduced inflammation and wound re-epithelialization of KPC-infected rats. These findings demonstrated that ZnO-NPs have great potential to be developed as antibacterial agents.

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Evaluation of Anti Carbapenem-Resistant Klebsiella Pneumonia of Zinc Oxide Nanoparticles Synthesized by Aspergillus Niger in Vitro and in Vivo

10.21203/rs.3.rs-757409/v1 ◽

2021 ◽

Author(s):

Elsayim Rasha ◽

Alkhulaifi Manal ◽

AlOthman Monerah ◽

Ibrahim Khalid ◽

Elnagar Doaa ◽

...

Keyword(s):

Wound Healing ◽

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Klebsiella Pneumonia ◽

Oxide Nanoparticles ◽

Zno Nps ◽

X Ray ◽

Carbapenem Resistant

Abstract Currently, the mortality rate is increasing in Saudi Arabia's ICU, due to the spread of KPC. This project was carried out to evaluate the ability of the biological synthesized of zinc Oxide nanoparticles (ZnO-NPs) using Aspergillus niger to overcome Carbapenem-Resistant Klebsiella pneumonia (KPC) in vitro and in vivo. ZnO-NPs was synthesized via a biological method and characterized using UV-Vis spectroscopy, Zeta sizer and Zeta potential analyses, X-ray diffraction (XRD) spectroscopy, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDX). In vitro sensitivity of KPC to ZnO-NPs was identified using the well diffusion method, MIC and MBC was determined by macro dilution method. The morphological alteration of KPC cell after ZnO-NPs treatment was showed by SEM. In vivo susceptibility of KPC to ZnO-NPs ointment was evaluated using wound healing in experimental rats. The chemical characterization findings showed the formation, stability, shape and size of the synthesized nanoparticles. The MIC and MBC results was found in 0.7mg\ml and 1.8mg\ml respectively. in vivo results displayed the inflammation reduction and wound healing re-epithelialisation of kpc infected rats. these findings demonstrated that ZnO-NPs has great potential to be developed as antibacterial agents and wound healing.

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Green Synthesis of Zinc Oxide Nanoparticles (ZnO-NPs) Using Arthrospira platensis (Class: Cyanophyceae) and Evaluation of their Biomedical Activities

Nanomaterials ◽

10.3390/nano11010095 ◽

2021 ◽

Vol 11 (1) ◽

pp. 95

Author(s):

Ehab F. El-Belely ◽

Mohamed M. S. Farag ◽

Hanan A. Said ◽

Abeer S. Amin ◽

Ehab Azab ◽

...

Keyword(s):

Zinc Oxide ◽

Photoelectron Spectroscopy ◽

Zinc Oxide Nanoparticles ◽

Arthrospira Platensis ◽

Oxide Nanoparticles ◽

Zno Nps ◽

X Ray ◽

Vis Spectroscopy ◽

Ft Ir

In this study, zinc oxide nanoparticles (ZnO-NPs) were successfully fabricated through the harnessing of metabolites present in the cell filtrate of a newly isolated and identified microalga Arthrospira platensis (Class: Cyanophyceae). The formed ZnO-NPs were characterized by UV–Vis spectroscopy, Fourier transform infrared (FT-IR), transmission electron microscopy (TEM), energy-dispersive spectroscopy (EDX), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). Data showed the efficacy of cyanobacterial metabolites in fabricating spherical, crystallographic ZnO-NPs with a size ≈30.0 to 55.0 nm at a wavelength of 370 nm. Moreover, FT-IR analysis showed varied absorption peaks related to nanoparticle formation. XPS analysis confirms the presence of Zn(II)O at different varied bending energies. Data analyses exhibit that the activities of biosynthesized ZnO-NPs were dose-dependent. Their application as an antimicrobial agent was examined and formed clear zones, 24.1 ± 0.3, 21.1 ± 0.06, 19.1 ± 0.3, 19.9 ± 0.1, and 21.6 ± 0.6 mm, at 200 ppm against Bacillus subtilis, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans, respectively, and these activities were reduced as the NPs concentration decreased. The minimum inhibitory concentration (MIC) values were determined as 50 ppm for S. aureus, 25 ppm for P. aeruginosa, and 12.5 ppm for B. subtilis, E. coli, and C. albicans. More interestingly, ZnO-NPs exhibit high in vitro cytotoxic efficacy against cancerous (Caco-2) (IC50 = 9.95 ppm) as compared with normal (WI38) cell line (IC50 = 53.34 ppm).

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Role of Zinc Oxide Nanoparticles Synthesized by Fenugreek Seeds Extract as Anticancer Agent: In Vitro and In Vivo Studies

Anti-Cancer Agents in Medicinal Chemistry ◽

10.2174/1871520621666210126093028 ◽

2021 ◽

Vol 21 ◽

Author(s):

Aliaa M. Radwan ◽

Eman F. Aboelfetoh ◽

Tetsunari Kimura ◽

Tarek M. Mohamed ◽

Mai M. El-Keiy

Keyword(s):

Zinc Oxide ◽

Electron Microscope ◽

Zinc Oxide Nanoparticles ◽

In Vivo Studies ◽

Oxide Nanoparticles ◽

Zno Nps ◽

Fenugreek Seeds ◽

X Ray

Background: Zinc oxide nanoparticles (ZnO NPs) are one of the metal oxide nanoparticles, which have attracted the interest of the researchers due to their biocompatibility, easily surface functionalization, and cancer targeting. Objective: This study was designated to investigate the potential antitumor activity of the biologically synthesized ZnO NPs alone or in combination with doxorubicin using Ehrlich ascites carcinoma (EAC) model. Methods: In this study, ZnO NPs were prepared by green approach using fenugreek seeds extract as reducing and capping agent then characterized by scanning electron microscope (SEM), energy dispersive x-ray (EDX), X-ray diffraction (XRD), UV-V spectroscopy, and transmission electron microscope (TEM). The prepared nanoparticles were tested for in vitro and in vivo studies using different parameters. Results: Zinc oxide nanoparticles were determined to have cytotoxicity against different cancer cell lines with lower toxicity against normal one. Moreover, the in vivo study, demonstrated that the intraperitoneal injection of ZnO NPs alone or combined with doxorubicin in EAC mice inhibited the proliferation and growth of EAC by decreasing the ascetic volume and viable tumor cell count. This anti-proliferative efficiency of ZnO NPs was due to cell cycle arrest at G0/G1 phase and induction of apoptosis via upregulating the expression of caspase-3 and Bax and downregulating the expression of Bcl-2. Conclusion: Our findings indicated that the biologically synthesized ZnO NPs may be a promising nanomedicine therapy for cancer treatment in the future.

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Leonotis Nepetifolia Mediated Eco-Friendly Synthesis of Zno Nps: Photocatalytic, Antioxidant Activities and Their Applications to Nano-Composite Electrode Material for Supercapacitor

10.21203/rs.3.rs-602808/v1 ◽

2021 ◽

Author(s):

Gajendran Pavithra ◽

Santhakumar kannappan

Keyword(s):

Electron Microscopy ◽

Zinc Oxide ◽

Zno Nanoparticles ◽

Zinc Oxide Nanoparticles ◽

Antioxidant Activities ◽

Electrochemical Analysis ◽

Oxide Nanoparticles ◽

Zno Nps ◽

Pure Zinc ◽

X Ray

Abstract The present paper describes the green synthesis of Zinc oxide nanoparticles (ZnO NPs) from the flowers of L. nepetifolia. The synthesis of ZnO nanoparticles and examined by using Ultraviolet-visible spectroscopy, Fourier Transform-Infrared spectroscopy, X-ray Diffraction analysis, Dynamic Light Scattering analysis, Raman spectroscopy, Scanning Electron Microscopy and Energy Dispersive X-ray spectroscopy, Transmission Electron Microscopy, and Thermogravimetric Analysis. The photocatalytic studies were followed using methylene blue (MB) dye by ZnO nanoparticles by using sunlight as a source. The degradation of MB dye is found to be 90 %. Then the synthesized ZnO nanoparticles help to evaluate the antioxidant activities. The antioxidant activities of ZnO nanoparticles were exhibiting through scavenging of Nitric oxide, Hydrogen peroxide, and DPPH free radicals. Furthermore, the electrochemical analysis of reduced Graphene Oxide-Zinc oxide (rGO-ZnO) nanocomposite shows that the prepared rGO-ZnO nanocomposite has a high specific capacitance of about 667 F g-1 in comparison with the pure Zinc oxide nanoparticles (200 F g-1) and has good cycling stability of around 1000 cycles. The synthesis of multifunctional ZnO nanoparticles by using natural plant products like seeds, leaves, flowers, etc may help to explore as environment-friendly which is opposite to chemical synthesis.

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Green Wastes Mediated Zinc Oxide Nanoparticles: Synthesis, Characterization and Electrochemical Studies

Materials ◽

10.3390/ma13194241 ◽

2020 ◽

Vol 13 (19) ◽

pp. 4241

Author(s):

Enyioma C. Okpara ◽

Omolola E. Fayemi ◽

El-Sayed M. Sherif ◽

Harri Junaedi ◽

Eno E. Ebenso

Keyword(s):

Electron Microscopy ◽

Zinc Oxide ◽

Zno Nanoparticles ◽

Zinc Oxide Nanoparticles ◽

Catalytic Properties ◽

Oxide Nanoparticles ◽

Zno Nps ◽

Tem Analysis ◽

X Ray ◽

Citrus Peels

Zinc Oxide (ZnO) nanoparticles were prepared using a simple green synthesis approach in an alkaline medium, from three different extracts of citrus peels waste. The synthesized nano-crystalline materials were characterized by using ultraviolet-visible spectroscopy (UV-vis), x-ray powder diffraction (XRD), Fourier-transform infrared spectroscopy (FTIR), energy-dispersive x-ray spectroscopy (EDS), environmental scanning electron microscopy (ESEM), and transmission electron microscopy (TEM). UV-vis analysis of the nanoparticles showed broad peaks around 360 nm for the ZnO NPs (Zinc oxide nanoparticles) from three citrus peels’ extracts. ZnO NPs exhibited Zn–O band close to 553 cm−1, which further verified the formation of the ZnO NPs. A bandgap of 3.26 eV, 3.20 eV and 3.30 eV was calculated for the ZnO NPs from grape (ZnO NPs/GPE), lemon (ZnO NPs/LPE), and orange (ZnO NPs/OPE) peels extract, respectively. The average grain sizes of the ZnO nanoparticles were evaluated to be 30.28 nm, 21.98 nm, and 18.49 nm for grape (ZnO NPs/GPE), lemon (ZnO NPs/LPE), and orange (ZnO NPs/OPE) peel extract, respectively. The surface morphology and sizes of the nanoparticle were confirmed by ESEM and TEM analysis, respectively. Furthermore, the zeta potential of the as-prepared ZnO NPs from OPE, LPE, and GPE was −34.2 mV, −38.8 mV, and −42.9 mV, respectively, indicating the high stability of the nanoparticles. Cyclic voltammetric properties of the synthesized nanoparticles were investigated across extracts, and the results showed that the citrus peels extracts (CPE) mediated ZnO NPs modified screen plate carbon (SPC/ ZnO NPs/CPE) electrodes exhibited enhanced catalytic properties when compared with the bare SPCE. The electroactive areas computed from the enhancement of the bare SPCE was approximately three times for SPCE/ ZnO NPs/LPE, and SPCE/ZnO NPs/GPE, and two times for SPCE/ZnO NPs/OPE, higher than that of the bare SPCE. Comparison across the extracts suggested that the catalytic properties of the nanoparticles were unique in ZnO NPs from GPE.

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Cinnamomum tamala Leaf Extract Stabilized Zinc Oxide Nanoparticles: A Promising Photocatalyst for Methylene Blue Degradation

Nanomaterials ◽

10.3390/nano11061558 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1558

Author(s):

Sajina Narath ◽

Supin Karonnan Koroth ◽

Sarojini Sharath Shankar ◽

Bini George ◽

Vasundhara Mutta ◽

...

Keyword(s):

Electron Microscopy ◽

Methylene Blue ◽

Zinc Oxide ◽

Photoelectron Spectroscopy ◽

Zinc Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

Zno Nps ◽

X Ray ◽

Green Protocol ◽

Cinnamomum Tamala

A facile green synthetic method is proposed for the synthesis of zinc oxide nanoparticles (ZnO NPs) using the bio-template Cinnamomum tamala (C. tamala) leaves extract. The morphological, functional, and structural characterization of synthesized ZnO NPs were studied by adopting different techniques such as energy dispersive X-ray analysis (EDX), high-resolution transmission electron microscopy (HR-TEM), scanning electron microscopy (SEM), X-ray diffraction (XRD), UV-Visible spectroscopy, fourier transform infrared (FTIR) spectroscopy, raman spectroscopy, and X-ray photoelectron spectroscopy (XPS). The fabricated ZnO NPs exhibit an average size of 35 nm, with a hexagonal nanostructure. Further, the well-characterized ZnO NPs were employed for the photocatalytic degradation of methylene blue (MB) in an aqueous solution. The photocatalytic activity was analyzed by changing the various physicochemical factors such as reaction time, amount of photocatalyst, precursor concentration, and calcination temperature of the ZnO NPs. All the studies suggest that the ZnO synthesized through the green protocol exhibits excellent photocatalytic potency against the dye molecules.

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Scolicidal activity of biosynthesized zinc oxide nanoparticles by Mentha longifolia L. leaves against Echinococcus granulosus protoscolices

Emergent Materials ◽

10.1007/s42247-021-00264-9 ◽

2021 ◽

Author(s):

Bushra H. Shnawa ◽

Samir M. Hamad ◽

Azeez A. Barzinjy ◽

Payman A. Kareem ◽

Mukhtar H. Ahmed

Keyword(s):

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Public Health Problem ◽

Analytical Techniques ◽

Oxide Nanoparticles ◽

Zno Nps ◽

Leaf Extracts ◽

X Ray ◽

Morphological Alterations ◽

Mentha Longifolia

AbstractCystic echinococcosis is a public health problem in developing countries that practice sheep breeding extensively. In the current study, the protoscolicidal activity of biosynthesized zinc oxide nanoparticles (ZnO NPs) derived from Mentha longifolia L. leaf extracts was investigated. The resultant ZnO NPs were characterized by means of various analytical techniques, such as ultraviolet–visible (UV–Vis) spectrometry, Fourier transform infrared (FT-IR) spectrophotometry, X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX) analysis. The results showed that the ZnO NP had the highest scolicidal activity at 400 ppm concentration after 150 min of exposure time, showing 100% mortality rate. The treated protoscolices exhibited loss of viability with several morphological alterations. Hence, an easy and effective green synthesis of ZnO NPs, with efficient scolicidal potential, is reported in this study.

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Thermophysiological comfort of zinc oxide nanoparticles coated woven fabrics

Scientific Reports ◽

10.1038/s41598-020-78305-2 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Muhammad Tayyab Noman ◽

Michal Petru ◽

Nesrine Amor ◽

Petr Louda

Keyword(s):

Surface Roughness ◽

Zinc Oxide ◽

Surface Topography ◽

Zinc Oxide Nanoparticles ◽

Air Permeability ◽

Woven Fabrics ◽

Oxide Nanoparticles ◽

Zno Nps ◽

X Ray ◽

Moisture Management

AbstractThis study investigates physicochemical impact of ultrasonic irradiations on surface topography of woven fabrics. In a simultaneous in-situ sonochemical method, the synthesis and coating of zinc oxide nanoparticles (ZnO NPs) on woven textiles were successfully achieved. Different instruments i.e. Alambeta, moisture management tester, air permeability tester and permetester were utilised during experimentation for thermal evaluation, moisture transportation and air permeation. The results regarding thermophysiological comfort of ZnO coated fabrics were evaluated on the basis of thickness and ZnO NPs coated amount on fabrics. In addition, the achieved results depict the impact of sonication (pressure gradient) on surface roughness of cotton and polyester. The coating of ZnO NPs on fabrics, crystal phase identification, surface topography and fluctuations in surface roughness were estimated by inductively coupled plasma atomic emission spectroscopy (ICP-AES), X-ray Diffractometry (XRD), ultrahigh-resolution scanning electron microscopy (UHR-SEM) and energy dispersive X-ray (EDX). Moreover, thermophysiological properties i.e. thermal conductivity, absolute evaporative resistance, thermal absorptivity, air permeability, overall moisture management capacity and relative water vapour permeability of untreated and ZnO treated samples were evaluated by standard test methods.

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Bioactivities of Geranium wallichianum Leaf Extracts Conjugated with Zinc Oxide Nanoparticles

Biomolecules ◽

10.3390/biom10010038 ◽

2019 ◽

Vol 10 (1) ◽

pp. 38 ◽

Cited By ~ 21

Author(s):

Banzeer Ahsan Abbasi ◽

Javed Iqbal ◽

Riaz Ahmad ◽

Layiq Zia ◽

Sobia Kanwal ◽

...

Keyword(s):

Electron Microscopy ◽

Zinc Oxide ◽

Zinc Oxide Nanoparticles ◽

Energy Dispersive ◽

Oxide Nanoparticles ◽

X Ray Diffraction ◽

Leaf Extracts ◽

Tem Analysis ◽

X Ray ◽

Transmission Electron

This study attempts to obtain and test the bioactivities of leaf extracts from a medicinal plant, Geranium wallichianum (GW), when conjugated with zinc oxide nanoparticles (ZnONPs). The integrity of leaf extract-conjugated ZnONPs (GW-ZnONPs) was confirmed using various techniques, including Ultraviolet–visible spectroscopy, X-Ray Diffraction, Fourier Transform Infrared Spectroscopy, energy-dispersive spectra (EDS), scanning electron microscopy, transmission electron microscopy, and Raman spectroscopy. The size of ZnONPs was approximately 18 nm, which was determined by TEM analysis. Additionally, the energy-dispersive spectra (EDS) revealed that NPs have zinc in its pure form. Bioactivities of GW-ZnONPs including antimicrobial potentials, cytotoxicity, antioxidative capacities, inhibition potentials against α-amylase, and protein kinases, as well as biocompatibility were intensively tested and confirmed. Altogether, the results revealed that GW-ZnONPs are non-toxic, biocompatible, and have considerable potential in biological applications.

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