scholarly journals Rapid Detection of Cysticercus cellulosae by an Up-Converting Phosphor Technology-Based Lateral-Flow Assay

2021 ◽  
Vol 11 ◽  
Author(s):  
Dejia Zhang ◽  
Yu Qi ◽  
Yaxuan Cui ◽  
Weiyi Song ◽  
Xinrui Wang ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Rapid Detection ◽  
Small Volume ◽  
Taenia Solium ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Neglected Tropical Disease ◽  
Porcine Cysticercosis ◽  
Cysticercus Cellulosae ◽  
Higher Sensitivity

Cysticercosis is a neglected tropical disease caused by the larvae of Taenia solium in pigs and humans. The current diagnosis of porcine cysticercosis is difficult, and traditional pathological tests cannot meet the needs of detection. This study established a UPT-LF assay for the detection of Cysticercus cellulosae. UCP particles were bound to two antigens, TSOL18 and GP50; samples were captured, and the signal from the UCP particles was converted into a detectable signal for analysis using a biosensor. Compared to ELISA, UPT-LF has higher sensitivity and specificity, with a sensitivity of 93.59% and 97.44%, respectively, in the case of TSOL18 and GP50 antigens and a specificity of 100% for both. Given its rapidness, small volume, high sensitivity and specificity, and good stability and reproducibility, this method could be used in the diagnosis of cysticercosis.

scholarly journals Development of a Prototype Lateral Flow Immunoassay for Rapid Detection of Staphylococcal Protein A in Positive Blood Culture Samples

Diagnostics ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 794
Author(s):  
Arpasiri Srisrattakarn ◽  
Patcharaporn Tippayawat ◽  
Aroonwadee Chanawong ◽  
Ratree Tavichakorntrakool ◽  
Jureerut Daduang ◽  
...  
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Rapid Detection ◽  
Protein A ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Staphylococcal Protein A ◽  
Highly Sensitive ◽  
Sophisticated Equipment ◽  
Higher Sensitivity

Bloodstream infection (BSI) is a major cause of mortality in hospitalized patients worldwide. Staphylococcus aureus is one of the most common pathogens found in BSI. The conventional workflow is time consuming. Therefore, we developed a lateral flow immunoassay (LFIA) for rapid detection of S. aureus-protein A in positive blood culture samples. A total of 90 clinical isolates including 58 S. aureus and 32 non-S. aureus were spiked in simulated blood samples. The antigens were extracted by a simple boiling method and diluted before being tested using the developed LFIA strips. The results were readable by naked eye within 15 min. The sensitivity of the developed LFIA was 87.9% (51/58) and the specificity was 93.8% (30/32). When bacterial colonies were used in the test, the LFIA provided higher sensitivity and specificity (94.8% and 100%, respectively). The detection limit of the LFIA was 107 CFU/mL. Initial evaluation of the LFIA in 20 positive blood culture bottles from hospitals showed 95% agreement with the routine methods. The LFIA is a rapid, simple and highly sensitive method. No sophisticated equipment is required. It has potential for routine detection particularly in low resource settings, contributing an early diagnosis that facilitates effective treatment and reduces disease progression.

scholarly journals Evaluation of an Immunochromatographic Lateral Flow Assay (OXA-48K-SeT) for Rapid Detection of OXA-48-Like Carbapenemases in Enterobacteriaceae

2015 ◽  
Vol 54 (2) ◽  
pp. 471-473 ◽  
Author(s):  
David W. Wareham ◽  
Rishita Shah ◽  
Jonathan W. Betts ◽  
Lynette M. Phee ◽  
Muhd Haziq F. Abdul Momin
Keyword(s):  
Blood Culture ◽  
Sensitivity And Specificity ◽  
Rapid Detection ◽  
Solid Medium ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Content Type

We evaluated an immunochromatographic lateral flow assay to detect OXA-48-like carbapenemases (OXA-48K-SeT) inEnterobacteriaceae(n= 82). One hundred percent sensitivity and specificity were observed using bacteria recovered from both solid medium and spiked blood culture bottles, and the results were obtained in <10 min.

scholarly journals Rapid Detection of VanA/B-Producing Vancomycin-Resistant Enterococci Using Lateral Flow Immunoassay

Diagnostics ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 1805
Author(s):  
Saoussen Oueslati ◽  
Camille Gonzalez ◽  
Hervé Volland ◽  
Vincent Cattoir ◽  
Sandrine Bernabeu ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Rapid Detection ◽  
Clinical Microbiology ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Bacterial Cells ◽  
Efficient Management ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Prolonged Hospital

Vancomycin-resistant enterococci (VREs) have become one of the most important nosocomial pathogens worldwide, associated with increased treatment costs, prolonged hospital stays and high mortality. Rapid detection is crucial to reduce their spread and prevent infections and outbreaks. The lateral flow immunoassay NG-Test VanB (NG Biotech) was evaluated for the rapid detection of VanB-producing vancomycin-resistant enterococci (VanB-VREs) using 104 well-characterized enterococcal isolates. The sensitivity and specificity were both 100% when bacterial cells were grown in the presence of vancomycin used as a VanB inducer. The NG-Test VanB is an efficient, rapid and easy to implement assay in clinical microbiology laboratories for the confirmation of VanB-VREs from colonies. Together with the NG-Test VanA, they could replace the already existing tests available for the confirmation of acquired vancomycin resistance in enterococci, especially from selective media or from antibiograms, with 100% sensitivity and specificity. Rapid detection in less than 15 min will result in more efficient management of carriers and infected patients. In addition, these tests may be used for positive blood cultures, given a 3.5 h sub-culturing step on Chocolate agar PolyViteX in the presence of a 5-µg vancomycin disk, which is routinely performed in many clinical microbiology laboratories for every positive blood culture for subsequent MALDI-TOF identification of the growing bacteria.

Rapid detection of cow milk adulteration/contamination in goat milk by a lateral flow colloidal gold immunoassay strip

2019 ◽  
Vol 86 (1) ◽  
pp. 94-97 ◽  
Author(s):  
Bochao Liu ◽  
Jinhong Si ◽  
Fang Zhao ◽  
Qi Wang ◽  
Yu Wang ◽  
...  
Keyword(s):  
Colloidal Gold ◽  
Rapid Detection ◽  
Goat Milk ◽  
Lateral Flow ◽  
Cow Milk ◽  
Quantitative Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Milk Adulteration ◽  
Milk Casein ◽  
Higher Sensitivity

AbstractCurrent available methods to detect cow milk adulteration or accidental contamination of goat milk are both laborious and time consuming. The aim of this technical research communication was to develop a simple, rapid, specific and sensitive method for quantitative detection of cow milk in goat milk. A competitive lateral flow immunoassay (LFIA) strip was developed using a specific monoclonal antibody (mAb) labeled with colloidal gold nanoparticles (GNPs) for specifically binding to cow milk casein. The detection limit of this rapid detection was 0.07% of cow milk in goat milk, providing equal specificity and higher sensitivity when compared with a commercial enzyme-linked immunosorbent assay (ELISA). These result suggest that the established rapid GNPs-LFIA strip could be used for monitoring cow milk adulteration/contamination of goat milk.

A water-soluble dual-site fluorescent probe for the rapid detection of cysteine with high sensitivity and specificity

2019 ◽  
Vol 55 (78) ◽  
pp. 11762-11765 ◽  
Author(s):  
Bao-Jun Wang ◽  
Rui-Juan Liu ◽  
Jianguo Fang ◽  
Ya-Wen Wang ◽  
Yu Peng
Keyword(s):  
Fluorescent Probe ◽  
Sensitivity And Specificity ◽  
Rapid Detection ◽  
High Sensitivity ◽  
Water Soluble ◽  
Specific Detection ◽  
Turn On ◽  
Dual Site

A water-soluble turn-on fluorescent probe has been developed for the rapid, sensitive and specific detection of Cys.

scholarly journals Multiantigen Print Immunoassay for Comparison of Diagnostic Antigens for Taenia solium Cysticercosis and Taeniasis

2009 ◽  
Vol 17 (1) ◽  
pp. 68-72 ◽  
Author(s):  
Sukwan Handali ◽  
Molly Klarman ◽  
Amanda N. Gaspard ◽  
John Noh ◽  
Yeuk-Mui Lee ◽  
...  
Keyword(s):  
Schistosoma Mansoni ◽  
Sensitivity And Specificity ◽  
Rapid Detection ◽  
Taenia Solium ◽  
Cross Reactivity ◽  
Antibody Responses ◽  
Immunoblot Assay ◽  
Clinical Presentations ◽  
Lentil Lectin ◽  
The Brain

ABSTRACT One of the best-characterized tests for the diagnosis of neurocysticercosis is the enzyme-linked immunoelectrotransfer blot assay, developed at the CDC, which uses lentil lectin-purified glycoproteins (LLGPs) extracted from Taenia solium cysticerci. The purification of the LLGP antigens has been difficult to standardize, and the polyacrylamide gel system used for the immunoblot assay is not easily transferable to other laboratories. In this study, we developed a multiantigen printing immunoassay (MAPIA) to compare the performance of multiple recombinant Taenia solium proteins with the potential for the detection of cysticercosis and taeniasis. We prepared MAPIA strips using six cysticercosis and two taeniasis diagnostic proteins and compared the performance of the proteins with sera collected from defined cysticercosis and taeniasis cases. Of the six cysticercosis antigens, rT24H performed well in detecting cases with two or more viable cysts in the brain (sensitivity and specificity, 97% and 99.4%, respectively); the use of a combination of cysticercosis antigens did not improve the sensitivity of the test and decreased the specificity. None of the antigens could differentiate the different clinical presentations of cysticercosis. Both of the taeniasis antigens (rES33 and rES38) had the same sensitivity of 99.4% and specificities of 93.9% and 94.5%, respectively. Some cross-reactivity against rES33 and rES38 was found, especially with sera from cases infected with Schistosoma mansoni. We conclude that MAPIA is a simple and effective tool that may be used to compare antibody responses to different cysticercosis and taeniasis antigens and, in this case, may be useful for the rapid detection of T. solium cases.

scholarly journals Is Procalcitonin Useful in Early Diagnosis of Serious Bacterial Infections in Children?

2013 ◽  
Vol 33 (2) ◽  
pp. 106-109
Author(s):  
Rakesh Gupta ◽  
Veena Singh ◽  
Seema Patrikar ◽  
Nandita Hazra ◽  
S S Mathai
Keyword(s):  
Sensitivity And Specificity ◽  
Bacterial Infections ◽  
Medical Science ◽  
Clinical Signs ◽  
High Sensitivity ◽  
Peripheral Blood Smear ◽  
Total Leukocyte Count ◽  
Serious Bacterial Infections ◽  
Sepsis Screen ◽  
Higher Sensitivity

Introduction: Diagnosis of bacterial infections remains one of the greatest challenges in medical science, especially in children, in whom clinical signs are often nonspecific. The currently used sepsis screen has poor predictive value. Recently introduced marker procalcitonin (PCT) with high sensitivity and specificity is evaluated as early marker of serious bacterial infection in children. Materials and Methods: Children up to 5 years of age presenting with features of Systemic Inflammatory Response Syndrome(SIRS) were evaluated clinically and underwent standard sepsis screen namely total leukocyte count (TLC), peripheral blood smear for band count, C-reactive Protein (CRP) and newer tests like procalcitonin (PCT) and Interleukin-8 (IL-8). Results were analyzed using SPSS14.0. Results: One hundred patients suspected of sepsis were evaluated. Maximum cases were below one year (37%) with mean age of 27 months. Male:female ratio was 1.5:1. Respiratory system was the commonest system involved in (54%) followed by gastrointestinal (20%), genitourinary (10%) and central nervous system (5%). Seventy two cases were found to have confirmed sepsis, proven by blood culture (34%) and other investigations. Fifty two cases were diagnosed by conventional markers, while newer markers in 60 cases. Diagnostic evaluation revealed that newer markers have higher sensitivity and specificity as compared to conventional sepsis screen. Conclusion: Procalcitonin is a useful marker for diagnosis of serious bacterial infections in children and in combination with IL8 has a higher sensitivity and specificity as compared to standard sepsis screen. Therefore it is recommended that procalcitonin should be used for the screening of sepsis in children so that the treatment can be started earlier in order to prevent morbidity and mortality. DOI: http://dx.doi.org/10.3126/jnps.v33i2.7988 J Nepal Paediatr Soc. 2013; 33(2):106-109

scholarly journals Rapid Detection of VanA/B-Producing Vancomycin-Resistant enterococci using Lateral Flow Immunoassay from colonies and blood culture

2020 ◽  
Author(s):  
Saoussen Oueslati ◽  
Hervé Volland ◽  
Vincent Cattoir ◽  
Sandrine Bernabeu ◽  
Delphine Girlich ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Rapid Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Bacterial Cells ◽  
Prolonged Hospital Stay ◽  
Efficient Management ◽  
Vancomycin Resistant Enterococci ◽  
Vancomycin Resistant ◽  
Prolonged Hospital

AbstractVancomycin-resistant enterococci (VRE) have become one of the most important nosocomial pathogens worldwide associated with increased treatment costs, prolonged hospital stay, and high mortality. Rapid detection is crucial to reduce their spread and prevent infections and outbreaks. The lateral flow immunoassay NG-Test VanB (NG Biotech) was evaluated for the rapid detection of VanB-producing vancomycin-resistant enterococci (VanB-VRE) using 104 well-characterized enterococcal isolates. The sensitivity and specificity were both 100%, when bacterial cells were grown in the presence of vancomycin used as VanB inducer. The NG-Test VanB is an efficient, rapid, and easy to implement assay in clinical microbiology laboratories for the confirmation of VanB-VREs either from colonies or from positive blood cultures. Together with the NG-Test VanA, they could complete/replace the already existing panel of tests available for confirmation of acquired vancomycin resistance in enterococci, especially from selective media (rectal screenings) or from antibiograms (infections), with a sensitivity and specificity of both of 100%. The rapid detection in less than 15 minutes will result in more efficient management of carriers and infected patients.

A Variety of Bio-nanogold in the Fabrication of Lateral Flow Biosensors for the Detection of Pathogenic Bacteria

2019 ◽  
Vol 19 (27) ◽  
pp. 2476-2493 ◽  
Author(s):  
Nan Cheng ◽  
Zhansen Yang ◽  
Weiran Wang ◽  
Xinxian Wang ◽  
Wentao Xu ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Pathogenic Bacteria ◽  
Limit Of Detection ◽  
Low Cost ◽  
High Sensitivity ◽  
Lateral Flow ◽  
Special Focus ◽  
Assay Performance ◽  
Performance Ability ◽  
Critical Overview

Pathogenic bacteria constitute one of the most serious threats to human health. This has led to the development of technologies for the rapid detection of bacteria. Bio-nanogold-based lateral flow biosensors (LFBs) are a promising assay due to their low limit of detection, high sensitivity, good selectivity, robustness, low cost, and quick assay performance ability. The aim of this review is to provide a critical overview of the current variety of bio-nanogold LFBs and their targets, with a special focus on whole-cell and DNA detection of pathogenic bacteria. The challenges of bio-nanogold-based LFBs in improving their performance and accessibility are also comprehensively discussed.

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