Rapid Detection of VanA/B-Producing Vancomycin-Resistant enterococci using Lateral Flow Immunoassay from colonies and blood culture

AbstractVancomycin-resistant enterococci (VRE) have become one of the most important nosocomial pathogens worldwide associated with increased treatment costs, prolonged hospital stay, and high mortality. Rapid detection is crucial to reduce their spread and prevent infections and outbreaks. The lateral flow immunoassay NG-Test VanB (NG Biotech) was evaluated for the rapid detection of VanB-producing vancomycin-resistant enterococci (VanB-VRE) using 104 well-characterized enterococcal isolates. The sensitivity and specificity were both 100%, when bacterial cells were grown in the presence of vancomycin used as VanB inducer. The NG-Test VanB is an efficient, rapid, and easy to implement assay in clinical microbiology laboratories for the confirmation of VanB-VREs either from colonies or from positive blood cultures. Together with the NG-Test VanA, they could complete/replace the already existing panel of tests available for confirmation of acquired vancomycin resistance in enterococci, especially from selective media (rectal screenings) or from antibiograms (infections), with a sensitivity and specificity of both of 100%. The rapid detection in less than 15 minutes will result in more efficient management of carriers and infected patients.

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Rapid Detection of VanA/B-Producing Vancomycin-Resistant Enterococci Using Lateral Flow Immunoassay

Diagnostics ◽

10.3390/diagnostics11101805 ◽

2021 ◽

Vol 11 (10) ◽

pp. 1805

Author(s):

Saoussen Oueslati ◽

Camille Gonzalez ◽

Hervé Volland ◽

Vincent Cattoir ◽

Sandrine Bernabeu ◽

...

Keyword(s):

Sensitivity And Specificity ◽

Rapid Detection ◽

Clinical Microbiology ◽

Lateral Flow ◽

Lateral Flow Immunoassay ◽

Bacterial Cells ◽

Efficient Management ◽

Vancomycin Resistant Enterococci ◽

Vancomycin Resistant ◽

Prolonged Hospital

Vancomycin-resistant enterococci (VREs) have become one of the most important nosocomial pathogens worldwide, associated with increased treatment costs, prolonged hospital stays and high mortality. Rapid detection is crucial to reduce their spread and prevent infections and outbreaks. The lateral flow immunoassay NG-Test VanB (NG Biotech) was evaluated for the rapid detection of VanB-producing vancomycin-resistant enterococci (VanB-VREs) using 104 well-characterized enterococcal isolates. The sensitivity and specificity were both 100% when bacterial cells were grown in the presence of vancomycin used as a VanB inducer. The NG-Test VanB is an efficient, rapid and easy to implement assay in clinical microbiology laboratories for the confirmation of VanB-VREs from colonies. Together with the NG-Test VanA, they could replace the already existing tests available for the confirmation of acquired vancomycin resistance in enterococci, especially from selective media or from antibiograms, with 100% sensitivity and specificity. Rapid detection in less than 15 min will result in more efficient management of carriers and infected patients. In addition, these tests may be used for positive blood cultures, given a 3.5 h sub-culturing step on Chocolate agar PolyViteX in the presence of a 5-µg vancomycin disk, which is routinely performed in many clinical microbiology laboratories for every positive blood culture for subsequent MALDI-TOF identification of the growing bacteria.

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An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

SLAS TECHNOLOGY Translating Life Sciences Innovation ◽

10.1177/2472630320981935 ◽

2021 ◽

pp. 247263032098193

Author(s):

Cheng Liu ◽

Shuiqin Fang ◽

Yachen Tian ◽

Youxue Wu ◽

Meijiao Wu ◽

...

Keyword(s):

Escherichia Coli ◽

Rapid Detection ◽

Foodborne Pathogen ◽

Test Strip ◽

Lateral Flow ◽

Detection Sensitivity ◽

Lateral Flow Immunoassay ◽

Escherichia Coli O157 ◽

Aggregation Induced Emission ◽

E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

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Evaluation of GeneXpert vanA/vanB in the early diagnosis of vancomycin-resistant enterococci infection

PLoS Neglected Tropical Diseases ◽

10.1371/journal.pntd.0009869 ◽

2021 ◽

Vol 15 (11) ◽

pp. e0009869

Author(s):

Zhuo-Lei Li ◽

Qi-Bing Luo ◽

Shan-Shan Xiao ◽

Ze-Hong Lin ◽

Ye-Ling Liu ◽

...

Keyword(s):

Likelihood Ratio ◽

Sensitivity And Specificity ◽

Characteristic Curve ◽

Positive Likelihood Ratio ◽

Negative Likelihood Ratio ◽

Clinical Problem ◽

Diagnostic Odds Ratio ◽

Cochrane Library ◽

Vancomycin Resistant Enterococci ◽

Vancomycin Resistant

Purpose Vancomycin-resistant enterococci infection is a worrying worldwide clinical problem. To evaluate the accuracy of GeneXpert vanA/vanB in the diagnosis of VRE, we conducted a systematic review in the study. Methods Experimental data were extracted from publications until May 03 2021 related to the diagnostic accuracy of GeneXpert vanA/vanB for VRE in PubMed, Embase, Web of Science and the Cochrane Library. The accuracy of GeneXpert vanA/vanB for VRE was evaluated using summary receiver to operate characteristic curve, pooled sensitivity, pooled specificity, positive likelihood ratio, negative likelihood ratio, and diagnostic odds ratio. Results 8 publications were divided into 3 groups according to two golden standard references, vanA and vanB group, vanA group, vanB group, including 6 researches, 5 researches and 5 researches, respectively. The pooled sensitivity and specificity of group vanA and vanB were 0.96 (95% CI, 0.93–0.98) and 0.90 (95% CI, 0.88–0.91) respectively. The DOR was 440.77 (95% CI, 37.92–5123.55). The pooled sensitivity and specificity of group vanA were 0.86 (95% CI, 0.81–0.90) and 0.99 (95% CI, 0.99–0.99) respectively, and those of group vanB were 0.85 (95% CI, 0.63–0.97) and 0.82 (95% CI, 0.80–0.83) respectively. Conclusion GeneXpert vanA/vanB can diagnose VRE with high-accuracy and shows greater accuracy in diagnosing vanA.

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A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci

Annals of Saudi Medicine ◽

10.5144/0256-4947.2018.200 ◽

2018 ◽

Vol 38 (3) ◽

pp. 200-207 ◽

Cited By ~ 3

Author(s):

Hani Adnan Ozbak

Keyword(s):

High Resolution ◽

Rapid Detection ◽

High Resolution Melting ◽

High Resolution Melting Analysis ◽

Analysis Approach ◽

Vancomycin Resistant Enterococci ◽

Melting Analysis ◽

Vancomycin Resistant

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Rapid detection of Vancomycin-Resistant Enterococci (VRE) in rectal samples from patients admitted to intensive care units

The Brazilian Journal of Infectious Diseases ◽

10.1590/s1413-86702009000400010 ◽

2009 ◽

Vol 13 (4) ◽

pp. 289-293 ◽

Cited By ~ 6

Author(s):

Pedro Alves d'Azevedo ◽

Kelly Aline de Souza Santiago ◽

Guilherme Henrique Campos Furtado ◽

Diego Batista Xavier ◽

Antonio Carlos Campos Pignatari ◽

...

Keyword(s):

Intensive Care ◽

Intensive Care Units ◽

Rapid Detection ◽

Vancomycin Resistant Enterococci ◽

Vancomycin Resistant

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Development of a Prototype Lateral Flow Immunoassay for Rapid Detection of Staphylococcal Protein A in Positive Blood Culture Samples

Diagnostics ◽

10.3390/diagnostics10100794 ◽

2020 ◽

Vol 10 (10) ◽

pp. 794

Author(s):

Arpasiri Srisrattakarn ◽

Patcharaporn Tippayawat ◽

Aroonwadee Chanawong ◽

Ratree Tavichakorntrakool ◽

Jureerut Daduang ◽

...

Keyword(s):

Blood Culture ◽

Positive Blood Culture ◽

Rapid Detection ◽

Protein A ◽

Lateral Flow ◽

Lateral Flow Immunoassay ◽

Staphylococcal Protein A ◽

Highly Sensitive ◽

Sophisticated Equipment ◽

Higher Sensitivity

Bloodstream infection (BSI) is a major cause of mortality in hospitalized patients worldwide. Staphylococcus aureus is one of the most common pathogens found in BSI. The conventional workflow is time consuming. Therefore, we developed a lateral flow immunoassay (LFIA) for rapid detection of S. aureus-protein A in positive blood culture samples. A total of 90 clinical isolates including 58 S. aureus and 32 non-S. aureus were spiked in simulated blood samples. The antigens were extracted by a simple boiling method and diluted before being tested using the developed LFIA strips. The results were readable by naked eye within 15 min. The sensitivity of the developed LFIA was 87.9% (51/58) and the specificity was 93.8% (30/32). When bacterial colonies were used in the test, the LFIA provided higher sensitivity and specificity (94.8% and 100%, respectively). The detection limit of the LFIA was 107 CFU/mL. Initial evaluation of the LFIA in 20 positive blood culture bottles from hospitals showed 95% agreement with the routine methods. The LFIA is a rapid, simple and highly sensitive method. No sophisticated equipment is required. It has potential for routine detection particularly in low resource settings, contributing an early diagnosis that facilitates effective treatment and reduces disease progression.

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Development of a nucleic acid lateral flow immunoassay (NALFIA) for reliable, simple and rapid detection of the methicillin resistance genes mecA and mecC

Veterinary Microbiology ◽

10.1016/j.vetmic.2016.08.009 ◽

2017 ◽

Vol 200 ◽

pp. 101-106 ◽

Cited By ~ 10

Author(s):

Constanze Seidel ◽

Sonja Peters ◽

Erik Eschbach ◽

Andrea T. Feßler ◽

Boris Oberheitmann ◽

...

Keyword(s):

Nucleic Acid ◽

Resistance Genes ◽

Rapid Detection ◽

Methicillin Resistance ◽

Lateral Flow ◽

Lateral Flow Immunoassay

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A Lateral Flow Immunoassay for the Rapid Detection of Ochratoxin A in Wine and Grape Must

Journal of Agricultural and Food Chemistry ◽

10.1021/jf3031666 ◽

2012 ◽

Vol 60 (46) ◽

pp. 11491-11497 ◽

Cited By ~ 41

Author(s):

Laura Anfossi ◽

Cristina Giovannoli ◽

Gianfranco Giraudi ◽

Flavia Biagioli ◽

Cinzia Passini ◽

...

Keyword(s):

Ochratoxin A ◽

Rapid Detection ◽

Lateral Flow ◽

Lateral Flow Immunoassay ◽

Grape Must

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Evaluation of an Immunochromatographic Lateral Flow Assay (OXA-48K-SeT) for Rapid Detection of OXA-48-Like Carbapenemases in Enterobacteriaceae

Journal of Clinical Microbiology ◽

10.1128/jcm.02900-15 ◽

2015 ◽

Vol 54 (2) ◽

pp. 471-473 ◽

Cited By ~ 32

Author(s):

David W. Wareham ◽

Rishita Shah ◽

Jonathan W. Betts ◽

Lynette M. Phee ◽

Muhd Haziq F. Abdul Momin

Keyword(s):

Blood Culture ◽

Sensitivity And Specificity ◽

Rapid Detection ◽

Solid Medium ◽

Lateral Flow ◽

Lateral Flow Assay ◽

Content Type

We evaluated an immunochromatographic lateral flow assay to detect OXA-48-like carbapenemases (OXA-48K-SeT) inEnterobacteriaceae(n= 82). One hundred percent sensitivity and specificity were observed using bacteria recovered from both solid medium and spiked blood culture bottles, and the results were obtained in <10 min.

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