scholarly journals Development of a Prototype Lateral Flow Immunoassay for Rapid Detection of Staphylococcal Protein A in Positive Blood Culture Samples

Diagnostics ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 794
Author(s):  
Arpasiri Srisrattakarn ◽  
Patcharaporn Tippayawat ◽  
Aroonwadee Chanawong ◽  
Ratree Tavichakorntrakool ◽  
Jureerut Daduang ◽  
...  
Keyword(s):  
Blood Culture ◽  
Positive Blood Culture ◽  
Rapid Detection ◽  
Protein A ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Staphylococcal Protein A ◽  
Highly Sensitive ◽  
Sophisticated Equipment ◽  
Higher Sensitivity

Bloodstream infection (BSI) is a major cause of mortality in hospitalized patients worldwide. Staphylococcus aureus is one of the most common pathogens found in BSI. The conventional workflow is time consuming. Therefore, we developed a lateral flow immunoassay (LFIA) for rapid detection of S. aureus-protein A in positive blood culture samples. A total of 90 clinical isolates including 58 S. aureus and 32 non-S. aureus were spiked in simulated blood samples. The antigens were extracted by a simple boiling method and diluted before being tested using the developed LFIA strips. The results were readable by naked eye within 15 min. The sensitivity of the developed LFIA was 87.9% (51/58) and the specificity was 93.8% (30/32). When bacterial colonies were used in the test, the LFIA provided higher sensitivity and specificity (94.8% and 100%, respectively). The detection limit of the LFIA was 107 CFU/mL. Initial evaluation of the LFIA in 20 positive blood culture bottles from hospitals showed 95% agreement with the routine methods. The LFIA is a rapid, simple and highly sensitive method. No sophisticated equipment is required. It has potential for routine detection particularly in low resource settings, contributing an early diagnosis that facilitates effective treatment and reduces disease progression.

Small size nanoparticles—Co3O4 based lateral flow immunoassay biosensor for highly sensitive and rapid detection of furazolidone

Talanta ◽  
2020 ◽  
Vol 211 ◽  
pp. 120729
Author(s):  
Lihong Su ◽  
Lulu Wang ◽  
Xiaolin Yao ◽  
Xuechi Yin ◽  
Han Zhang ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Highly Sensitive

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

scholarly journals Multi-Quantum Dots-Embedded Silica-Encapsulated Nanoparticle-Based Lateral Flow Assay for Highly Sensitive Exosome Detection

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 768
Author(s):  
Hyung-Mo Kim ◽  
Chiwoo Oh ◽  
Jaehyun An ◽  
Seungki Baek ◽  
Sungje Bock ◽  
...  
Keyword(s):  
Quantum Dots ◽  
Limit Of Detection ◽  
Specific Binding ◽  
Calf Serum ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Uniform Size ◽  
Highly Sensitive ◽  
Wide Range ◽  
New Biomarkers

Exosomes are attracting attention as new biomarkers for monitoring the diagnosis and prognosis of certain diseases. Colorimetric-based lateral-flow assays have been previously used to detect exosomes, but these have the disadvantage of a high limit of detection. Here, we introduce a new technique to improve exosome detection. In our approach, highly bright multi-quantum dots embedded in silica-encapsulated nanoparticles (M–QD–SNs), which have uniform size and are brighter than single quantum dots, were applied to the lateral flow immunoassay method to sensitively detect exosomes. Anti-CD63 antibodies were introduced on the surface of the M–QD–SNs, and a lateral flow immunoassay with the M–QD–SNs was conducted to detect human foreskin fibroblast (HFF) exosomes. Exosome samples included a wide range of concentrations from 100 to 1000 exosomes/µL, and the detection limit of our newly designed system was 117.94 exosome/μL, which was 11 times lower than the previously reported limits. Additionally, exosomes were selectively detected relative to the negative controls, liposomes, and newborn calf serum, confirming that this method prevented non-specific binding. Thus, our study demonstrates that highly sensitive and quantitative exosome detection can be conducted quickly and accurately by using lateral immunochromatographic analysis with M–QD–SNs.

Highly sensitive multiplex lateral flow immunoassay of phytopathogens using Au@Pt nanoparticles as the colorimetric and catalytic label

2021 ◽  
Author(s):  
Vasily G. Panferov ◽  
Shyatesa C. Razo ◽  
Irina V. Safenkova ◽  
Anatoly V. Zherdev ◽  
Boris B. Dzantiev
Keyword(s):  
Pt Nanoparticles ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Highly Sensitive

Europium-doped-nanoparticle-based multiplexed lateral flow immunoassay for the quantitative and highly sensitive detection of enterotoxins

2021 ◽  
Author(s):  
F. Mousseau ◽  
C. Feraudet-Tarisse ◽  
S. Simon ◽  
T. Gacoin ◽  
C. Bouzigues ◽  
...  
Keyword(s):  
Lateral Flow ◽  
Sensitive Detection ◽  
Lateral Flow Immunoassay ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Post-assay growth of gold nanoparticles as a tool for highly sensitive lateral flow immunoassay. Application to the detection of potato virus X

2018 ◽  
Vol 185 (11) ◽  
Author(s):  
Vasily G. Panferov ◽  
Irina V. Safenkova ◽  
Anatoly V. Zherdev ◽  
Boris B. Dzantiev
Keyword(s):  
Gold Nanoparticles ◽  
Potato Virus ◽  
Potato Virus X ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Highly Sensitive

A highly sensitive dual-color lateral flow immunoassay for brucellosis using one-step synthesized latex microspheres

2019 ◽  
Vol 11 (22) ◽  
pp. 2937-2942 ◽  
Author(s):  
Mingsong Zhu ◽  
Yurui Jia ◽  
Lizhi Peng ◽  
Jifu Ma ◽  
Xiangru Li ◽  
...  
Keyword(s):  
Clinical Care ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Screening Methods ◽  
Step Method ◽  
Dual Color ◽  
Highly Sensitive ◽  
One Step

A lateral flow immunoassay was developed to improve clinical care compared with conventional brucellosis screening methods. Detection is dual-color in format using dyed, carboxyl-functionalized latex microspheres synthesized with a one-step method.

scholarly journals Validity of a single antibody-based lateral flow immunoassay depending on graphene oxide for highly sensitive determination of E. coli O157:H7 in minced beef and river water

2019 ◽  
Vol 297 ◽  
pp. 124965 ◽  
Author(s):  
Abdelrahim Hussein Abdelazeem Hassan ◽  
José Francisco Bergua ◽  
Eden Morales-Narváez ◽  
Arben Mekoçi
Keyword(s):  
Graphene Oxide ◽  
River Water ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
E Coli ◽  
Minced Beef ◽  
Highly Sensitive ◽  
Sensitive Determination
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