scholarly journals Effect of Temperature on Tooth Staining by 0.12% Chlorhexidine Gluconate

2021 ◽  
Vol 2 ◽  
Author(s):  
Clifton M. Carey ◽  
Arthur Yagudayev ◽  
Kerri Font
Keyword(s):  
Pilot Study ◽  
Black Tea ◽  
Anatomical Variations ◽  
Daily Basis ◽  
Chlorhexidine Gluconate ◽  
Effect Of Temperature ◽  
Different Temperatures ◽  
Unstimulated Saliva ◽  
Control Samples

Introduction: Natural color of dentin is affected by many variables, including anatomical variations, age, how much dentin is exposed, or how much enamel is covering the dentin. Chlorhexidine gluconate (CHX) has been observed to cause tooth staining, especially of exposed dentin. Risk factors for CHX staining include the amount of time for CHX utilization amongst others. Interestingly, the temperature of the rinse when used has been identified as a risk factor. However, no evidence of the effect of temperature is available in the literature. The purpose of this study was to determine the effect of temperature on dentin staining due to CHX exposure.Methods: Two studies were done. The first a pilot study at room temperature to determine the time needed to establish staining solutions, a method to evaluate stain intensity, and establish the time needed to stain dentin samples in vitro. The second study exposed dentin samples on a twice daily basis to a 1 min soak in CHX at different temperatures, followed by a period in an unstimulated saliva mixed with black tea mixture. Temperatures tested were 4, 23, 37 and 50°C. Control samples were exposed to only black tea and saliva (no CHX) and tested at 23°C.Results: The pilot study found that the combination of CHX and black tea causes dentin staining. From this data the sample size needed for the second experiment was calculated, requiring 12 samples per group. Sixty dentin samples were divided amongst 5 groups. The data from this study showed significant darkening of the dentin samples over 18 days. The 4 and 23°C CHX rinses resulted in significant staining compared to the control samples. The 37 and 50°C CHX rinses did not stain significantly more than the control samples.Conclusions: Chlorhexidine has the ability to cause tooth staining in the presence of chromogens such as those in black tea. Significant darkening was observed at lower temperatures (4 and 23°C) over 18 days, therefore dental professionals may wish to advise gently warming the CHX rinse toward 37°C prior to use to reduce the risk of staining.

scholarly journals Effect of temperature, CO2 and O2 on motility and mobility of Anisakidae larvae

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Aiyan Guan ◽  
Inge Van Damme ◽  
Frank Devlieghere ◽  
Sarah Gabriël
Keyword(s):  
Enzymatic Digestion ◽  
Infected Fish ◽  
Effect Of Temperature ◽  
Video Recordings ◽  
Different Temperatures ◽  
Semi Solid ◽  
Zoonotic Parasites ◽  
The Impact ◽  
Phosphate Buffered Saline

AbstractAnisakidae, marine nematodes, are underrecognized fish-borne zoonotic parasites. Studies on factors that could trigger parasites to actively migrate out of the fish are very limited. The objective of this study was to assess the impact of different environmental conditions (temperature, CO2 and O2) on larval motility (in situ movement) and mobility (migration) in vitro. Larvae were collected by candling or enzymatic digestion from infected fish, identified morphologically and confirmed molecularly. Individual larvae were transferred to a semi-solid Phosphate Buffered Saline agar, and subjected to different temperatures (6 ℃, 12 ℃, 22 ℃, 37 ℃) at air conditions. Moreover, different combinations of CO2 and O2 with N2 as filler were tested, at both 6 °C and 12 °C. Video recordings of larvae were translated into scores for larval motility and mobility. Results showed that temperature had significant influence on larval movements, with the highest motility and mobility observed at 22 ℃ for Anisakis spp. larvae and 37 ℃ for Pseudoterranova spp. larvae. During the first 10 min, the median migration of Anisakis spp. larvae was 10 cm at 22 ℃, and the median migration of Pseudoterranova spp. larvae was 3 cm at 37 ℃. Larval mobility was not significantly different under the different CO2 or O2 conditions at 6 °C and 12 ℃. It was concluded that temperature significantly facilitated larval movement with the optimum temperature being different for Anisakis spp. and Pseudoterranova spp., while CO2 and O2 did not on the short term. This should be further validated in parasite-infected/spiked fish fillets.

scholarly journals Effect of temperature on immunocompetence of the blue mussel (Mytilus edulis)

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Alexandre Beaudry ◽  
Marlène Fortier ◽  
Stéphane Masson ◽  
Michel Auffret ◽  
Pauline Brousseau ◽  
...  
Keyword(s):  
Blue Mussel ◽  
Lysozyme Activity ◽  
Effect Of Temperature ◽  
Gonad Maturation ◽  
Gland Weight ◽  
Immune Parameters ◽  
Serum Lysozyme ◽  
Different Temperatures ◽  
Maturation Stages

The blue mussel is a filter-feeding bivalve commonly used in ecotoxicological monitoring as a sentinel species. Due to climate change and the increase of temperature expected in marine environment, it is important to anticipate potential impacts on this species. The aim of this study was to investigate the immunocompetence of blue mussels acclimated to different temperatures and on the effects of increasing temperatures (5, 10 and 20°C). Different indices and gonad maturation stages were also determined throughout the experiments. Cell viability, phagocytosis, serum lysozyme activity and cyclooxygenase (COX) activity were evaluated as immune parameters. The cellular immunity was also evaluated after hemocytes exposure to various cadmium concentrations <em>in vitro</em>. The results obtained demonstrate modulation of hemocyte viability and the ability of these cells to phagocytize in absence of contaminants. After the exposure to cadmium, hemocytes showed greater viability at 5°C while maintaining a higher phagocytic competence. In addition, the lysozyme activity stayed stable at all tested temperatures, contrary to that of COX, which increased when the mussels were maintained at 20°C. The evaluation of indices demonstrated no reduction of general conditions during all the experiment despite the increase of temperature and the reduction of the digestive gland weight. Moreover, the lack of food does not affect gonad maturation and the spawning process.

Effect of Temperature and Safeners on Glutathione Levels and Glutathione S-Transferase Activity in Maize

1991 ◽  
Vol 46 (9-10) ◽  
pp. 856-860 ◽  
Author(s):  
Daniel L. Kunkel ◽  
John C. Steffens ◽  
Robin R. Bellinder
Keyword(s):  
Low Temperatures ◽  
Herbicide Tolerance ◽  
Effect Of Temperature ◽  
Transferase Activity ◽  
Glutathione S Transferase ◽  
Different Temperatures ◽  
Maize Plants ◽  
Incubation Temperatures ◽  
Gst Activity

Abstract Studies were conducted to determine the biochemical aspects of chloroacetamide injury to maize and the mechanism by which safeners maintain herbicide tolerance, even at reduced temperatures. The objectives of these studies were threefold: one, determine whether gluta­thione (GSH) content varies in maize plants grown at three different temperatures in safener-treated and non-treated plants; two, determine whether glutathione S-transferase (GST) activ­ity varies in plants grown at different temperatures; and three, determine if GSH activity is sensitive to low temperatures in vitro. The herbicide safeners CGA -154281 [4-(dichloroacetyl)-3,4-dihydro-3-methyl-2 H-1 ,4-benzoxazine] and dichlormid [2,2-dichloro-N,N-di-2-propenylacetamide] were used with metolachlor [2-chloro-N-(2-ethyl-6-methylphenyl)-n-(2-methoxy-1-methyl)acetamide] or acetochlor [2-chloro-N-(ethoxymethyl)-N-2-ethyl-6-methylphenyl)-acetamide], respectively, to determine the mechanisms of maize tolerance. CGA -154281 signifi­cantly increased GSH levels in maize seedlings grown at 27 °C compared to non-safened seed­lings, however significant differences were not seen at 17 or 37 °C. Dichlormid increased GSH levels by 1.6-fold at all growth temperatures. Both CGA -154281 and dichlormid increased GST activity significantly at all growth temperatures. The safener-induced GST activity was main­tained at in vitro incubation temperatures of 5 and 15 °C for acetochlor and metolachlor, re­spectively. In contrast, GST activity from non-safened tissue was essentially absent at these temperatures. Therefore, greater GST activity following safener treatment may result in higher levels of herbicide metabolism, even at low temperatures.

scholarly journals Effect of Temperature on Urediniospore Production and Germinability in Puccinia arachidis1

1994 ◽  
Vol 21 (2) ◽  
pp. 79-81
Author(s):  
P. V. Subba Rao ◽  
P. Subrahmanyam ◽  
D. McDonald
Keyword(s):  
Arachis Hypogaea ◽  
Effect Of Temperature ◽  
Optimal Temperature ◽  
Puccinia Arachidis ◽  
Arachis Hypogaea L ◽  
Different Temperatures ◽  
Percent Germination ◽  
Detached Leaves

Abstract Effect of temperature on urediniospore production in Puccinia arachidis was investigated under monocyclic infection using detached leaves of the susceptible peanut (Arachis hypogaea L.) cultivar TMV 2. Urediniospores produced at different temperatures were also examined for their germinability in vitro. The optimal temperature for urediniospore production was at about 20 and 25 C. Temperatures below 20 C or above 30 C were highly detrimental to urediniospore production. There were also marked differences in the percent germination of urediniospores produced at different temperatures. Urediniospores produced at 20 and 25 C showed the highest germination percentages. The interaction of temperature with urediniospore production and germinability is important in understanding the development of peanut rust epidemics.

scholarly journals Effects of Light, Temperature, and Leaf Wetness Duration on Daylily Rust

Plant Disease ◽  
2003 ◽  
Vol 87 (4) ◽  
pp. 442-445 ◽  
Author(s):  
D. S. Mueller ◽  
J. W. Buck
Keyword(s):  
Light Intensity ◽  
Effect Of Temperature ◽  
Leaf Wetness ◽  
Disease Development ◽  
Lesion Development ◽  
Leaf Wetness Duration ◽  
Different Temperatures ◽  
Controlled Environments ◽  
Incubation Temperatures

Experiments in controlled environments were completed to determine the influence of light intensity, temperature, and leaf wetness duration on daylily rust caused by Puccinia hemerocallidis. As light intensity increased, there was a significant decrease in urediniospore germination (R2 = 0.88 and Y = 96 - 0.6x). Urediniospores germinated in vitro between 7 and 34°C with an optimal temperature of 22 to 24°C. To test the effect of temperature on infection efficiency, plants were inoculated with urediniospores, incubated under high relative humidity at 4, 10, 22, 30, or 36°C, and then transferred to a greenhouse at 23°C for 15 days. Plants incubated at 22°C had an average of 13 lesions cm leaf-1. Incubation temperatures of 4, 10, 30, or 36°C resulted in less than 1.5 lesions cm leaf-1. Plants were inoculated, incubated at 22°C for 24 h, and then incubated at different temperatures for 15 days to test the effect of temperature on disease development. There were no significant differences in disease development at 22 and 30°C; however, there were significantly fewer lesions at 10°C and no lesions developed at 36°C within 15 days. Five to six h of leaf wetness were required for lesion development and as the duration of leaf wetness increased, there was a significant increase in the number of lesions that developed. These studies indicate that for disease development of P. hemerocallidis on daylily, temperatures around 22°C and 5 h of leaf wetness are required during infection. However, once a daylily is infected, disease development is not as sensitive to environmental conditions.

Effect of temperature on seed set and in vitro pollen germination in French beans (Phaseolus vulgaris)

1979 ◽  
Vol 19 (101) ◽  
pp. 725 ◽  
Author(s):  
PJ Farlow ◽  
DE Byth ◽  
NS Kruger
Keyword(s):  
Pollen Germination ◽  
Seed Set ◽  
Germination Percentage ◽  
French Bean ◽  
Hot Water ◽  
Effect Of Temperature ◽  
In Vitro Pollen Germination ◽  
French Beans ◽  
Different Temperatures

A technique for in vitro germination of French bean pollen was developed and the effect of temperature on gamete development, pollen germination and seed set investigated. Temperature had a profound effect on in vitro pollen germination percentage, pollen tube growth and bursting percentage. These were maximal at 7.2�C, 16.7�C and 38.3�C, respectively. In this study, pollen development was not affected at day/night temperatures of 16.1�/12.8�C, and ovule abortion was the cause of seed set failure at these temperatures. Hot water treatment (48�-44�C) of flowers caused failure of seed set due to pollen inviability. Consequently this technique may allow hybridization without emasculation in beans. Treatment of buds with hot water of different temperatures and in vitro pollen germination at high temperatures may have application as screening techniques for heat tolerance in French beans.

scholarly journals Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures

2004 ◽  
Vol 28 (5) ◽  
pp. 1087-1091 ◽  
Author(s):  
Leila Aparecida Salles Pio ◽  
José Darlan Ramos ◽  
Moacir Pasqual ◽  
Flavia Carvalho Santos ◽  
Keize Pereira Junqueira
Keyword(s):  
Culture Medium ◽  
Calcium Nitrate ◽  
Pollen Grain ◽  
Effect Of Temperature ◽  
In Vitro Germination ◽  
Stigma Receptivity ◽  
Open Flower ◽  
Different Temperatures ◽  
The Ideal

The study was carried out in order to evaluate the effect of temperature and in vitor stigma receptivity on regeneration of orange cultivar (Valência, Pêra and Natal) pollen. Two experiments were carried out, in the first the ideal temperature of germination was assessed. Pollen was obtained from flowers at the balloon stage and inoculated in culture medium with 10 g L-1 agar, 200 mg L-1 boric acid, 100 g L-1 sucrose, 800 mg L-1 calcium nitrate, pH adjusted to 6,5 and incubated in a BOD chamber at temperatures of 23, 24, 25, 26 and 27ºC during a 24-hour photoperiod. After 12 hours of incubation, the best temperature for pollen grain germination was 25ºC for all varieties. In a second experiment, in order to test the receptivity of the stigma, flowers were collected at different flowering stages: small bud, balloon and open flower. The stigmas were by immersion exposed to hydrogen peroxide (perioxidase reaction), 3% for 3 minutes. Through the technique of Zeisler (1938), better results were detected at the balloon stage with 80 to 100% receptivity, while for the open flower the receptivity presented maximum indexes.

scholarly journals The effect of temperature on pollen germination and pollen tube growth of pear cultivars

2016 ◽  
Vol 61 (4) ◽  
pp. 333-341 ◽  
Author(s):  
Aleksandar Radovic ◽  
Dragan Nikolic ◽  
Dragan Milatovic ◽  
Dejan Djurovic
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Tube Growth ◽  
Effect Of Temperature ◽  
Tube Length ◽  
In Vitro Pollen Germination ◽  
Different Temperatures ◽  
Pollen Tube Length

The effect of three different temperatures (8, 16 and 24?C) on pollen germination and length of pollen tube in vitro was investigated in four pear cultivars (?Butirra Precoce Morettini?, ?Williams?, ?Conference? and ?Abate Fetel?). The temperature showed a significant effect on in vitro pollen germination. The highest pollen germination was determined at a temperature of 24?C (53.25%), somewhat lower at 16?C (44.72%) and the lowest at 8?C (23.16%). The temperature effect was significantly more pronounced on the length of pollen tube. Pollen tube length was about three times higher at the temperatures of 16 and 24?C compared to 8?C. The temperature of 8?C was not sufficient for pollen germination and pollen tube growth in pear cultivars. However, temperatures of 16 and 24?C were optimal for pollen germination and pollen tube growth.

scholarly journals The effect of temperature on pollen germination and pollen tube growth of sour cherry cultivars

2014 ◽  
Vol 59 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Dragan Milatovic ◽  
Dragan Nikolic
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Germination ◽  
Pollen Grains ◽  
Sour Cherry ◽  
Tube Growth ◽  
Effect Of Temperature ◽  
Different Temperatures ◽  
Influence Of Temperature On

The study was carried out to determine the effect of three different temperatures (5, 15 and 25?C) on in vitro pollen germination and pollen tube growth of five sour cherry cultivars: ?Heimanns Konservenweichsel?, ?Kelleriis 14?, ?Oblacinska?, ?Rexelle? and ?Sumadinka?. Pollen germination a % agar % Temperature significantly affected pollen germination. High germination rates (50-70%) were obtained at both 15?C and 25?C. Satisfactory germination rates (42-51%) were also obtained at 5?C in some cultivars (?Rexelle?, ?Sumadinka? and ?Heimanns Konservenweichsel?). The influence of temperature on the pollen tube growth was more prominent. The length of pollen tubes was three to six times higher at 15?C and 25?C in comparison with 5?C. This has led to the conclusion that the temperature of 5?C, although it could be adequate for pollen germination, is not high enough for optimal pollen tube growth. was determined by germinating pollen grains in culture medium containing 0.7agar-and 15sucrose.

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