scholarly journals A Model and Simulation of the Influence of Temperature and Amplicon Length on Environmental DNA Degradation Rates: A Meta-Analysis Approach

2021 ◽  
Vol 9 ◽  
Author(s):  
Tatsuya Saito ◽  
Hideyuki Doi
Keyword(s):  
Endangered Species ◽  
Water Temperature ◽  
Species Distribution ◽  
Degradation Rate ◽  
Meta Analysis ◽  
Environmental Dna ◽  
Rare And Endangered Species ◽  
Amplicon Length ◽  
Degradation Rates ◽  
Rare And Endangered

Environmental DNA (eDNA) analysis can detect aquatic organisms, including rare and endangered species, in a variety of habitats. Degradation can influence eDNA persistence, impacting eDNA-based species distribution and occurrence results. Previous studies have investigated degradation rates and associated contributing factors. It is important to integrate data from across these studies to better understand and synthesize eDNA degradation in various environments. We complied the eDNA degradation rates and related factors, especially water temperature and amplicon lengths of the measured DNA from 28 studies, and subjected the data to a meta-analysis. In agreement with previous studies, our results suggest that water temperature and amplicon length are significantly related to the eDNA degradation rate. From the 95% quantile model simulation, we predicted the maximum eDNA degradation rate in various combinations of water temperature and amplicon length. Predicting eDNA degradation could be important for evaluating species distribution and inducing innovation (e.g., sampling, extraction, and analysis) of eDNA methods, especially for rare and endangered species with small population size.

scholarly journals Environmental DNA degradation simulation from water temperature and DNA fragment length: A meta-analysis approach

2020 ◽  
Author(s):  
Tatsuya Saito ◽  
Hideyuki Doi
Keyword(s):  
Endangered Species ◽  
Water Temperature ◽  
Fragment Length ◽  
Degradation Rate ◽  
Model Simulation ◽  
Environmental Dna ◽  
Related Factors ◽  
Rare And Endangered Species ◽  
Degradation Rates ◽  
Rare And Endangered

AbstractEnvironmental DNA (eDNA) analysis can detect aquatic organisms, including rare and endangered species, in a variety of habitats. The degradation of eDNA concentration is important to investigate their distribution and has also been experimentally evaluated. It is important to integrate these data to synthesize eDNA degradation in various environments. We collected the eDNA degradation rates and related factors, especially water temperature and fragment lengths of the measured DNA from 28 studies. Our results suggest that water temperature and fragment length are significantly related to the eDNA degradation rate. From the 95% quantile model simulation, we predicted the maximum eDNA degradation rate in various combinations of water temperature and fragment length. Predicting eDNA degradation could be important for evaluating species distribution and inducing innovation of eDNA methods, especially for rare and endangered species with lower DNA concentrations.

scholarly journals Degradation factors of environmental DNA evaluated by experiments and meta-analysis

2021 ◽  
Vol 4 ◽  
Author(s):  
Tatsuya Saito ◽  
Hideyuki Doi
Keyword(s):  
Water Temperature ◽  
Degradation Rate ◽  
Meta Analysis ◽  
Environmental Dna ◽  
Resident Species ◽  
Multiple Sources ◽  
Related Factors ◽  
Marine Habitats ◽  
Amplicon Length ◽  
Degradation Rates

Environmental DNA (eDNA) methods have been developed to detect organisms' distributions and abundance/biomass in various environments. eDNA degradation is critical for eDNA evaluation, but, the dynamics and mechanisms of eDNA degradation are largely unknown, especially when considering different eDNA sources, e.g., cell-derived and fragmental DNA. In this study, we conducted the degradation experiments (Saito and Doi 2020a) and a meta-analysis (Saito and Doi 2020b). Firstly, we experimentally evaluated the degradation rates of eDNA derived from multiple sources, including fragmental DNA (the DNA of internal positive control, IPC), free cells from Oncorhynchus kisutch, and the resident species (Saito and Doi 2020a). We conducted the experiments with pond and seawater to evaluate the differences between freshwater and marine habitats. Our results showed that eDNA derived from the both cells and fragmental DNA decreased exponentially in the both sea and pond samples. The degradation of eDNA from the resident species showed similar behavior to the cell-derived eDNA. As a meta-analysis, we complied the degradation rates of eDNA in laboratory experiment and field studies from 28 studies (Saito and Doi 2020b). We also collected the related factors, including water sources, water temperature, DNA regions, and PCR amplicon lengths of the measured DNA. Our results suggested that water temperature and amplicon length were significantly related to the degradation rate of eDNA. From the simulation based on the 95% quantile model, we predicted the maximum degradation rate of eDNA in various combinations of water temperature and PCR amplicon length.

Genetic diversity and genetic structure of the rare and endangered species, Primula ranunculoides

2014 ◽  
Vol 21 (5) ◽  
pp. 601-609
Author(s):  
Wang Deyun ◽  
Peng Jie ◽  
Chen Yajing ◽  
Lü Guosheng ◽  
Zhang Xiaoping ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Genetic Structure ◽  
Endangered Species ◽  
Rare And Endangered Species ◽  
Rare And Endangered

Geoecological analysis of the Russian Federation regions representation in the state program “Environmental Protection”

2020 ◽  
Vol 957 (3) ◽  
pp. 47-53
Author(s):  
E.A. Kravets
Keyword(s):  
Endangered Species ◽  
Environmental Protection ◽  
Russian Federation ◽  
The State ◽  
Volga River ◽  
Unequal Distribution ◽  
State Program ◽  
Rare And Endangered Species ◽  
Rare And Endangered ◽  
The Russian Federation

The author offers mapping and geoecological analysis of the Russian Federation regions presence in the state program “Environmental Protection”. The unequal distribution of the program’s targets and activities in different regions is revealed. A considerable number of relevant environmental problems for several mentioned regions have not been reflected in the program. It is important to increase the area of specially protected natural areas for a significant number of subjects of the Russian Federation. The status “part of the territory occupied by specially protected natural territories of Federal value in the total area of the subject of the Russian Federation” is recommended to be assigned all regions of Russia. Identification and elimination of objects of accumulated environmental damage that threat to the Volga river is relevant, at least for all the regions in which the Volga flows. Not all regions with a high level of air pollution and/or large masses of air pollutants have the indicator “reduction of total emissions for the reporting year”. It is necessary to increase the Program of measures for the protection of rare and endangered species of plants and to expand the list of regions in which it is planned to protect rare and endangered species of animals.

scholarly journals Research on a Biodiversity Conservation Value Assessment Method Based on Habitat Suitability of Species: A Case Study in Gansu Province, China

2021 ◽  
Vol 13 (6) ◽  
pp. 3007
Author(s):  
Xiaojiong Zhao ◽  
Jian Wang ◽  
Junde Su ◽  
Wei Sun ◽  
Haoxian Meng
Keyword(s):  
Ecosystem Services ◽  
Endangered Species ◽  
Biodiversity Conservation ◽  
Gansu Province ◽  
Distribution Area ◽  
Suitable Habitat ◽  
Conservation Value ◽  
Value Assessment ◽  
Rare And Endangered Species ◽  
Rare And Endangered

Quantitative assessment and evaluation of ecological parameters and biodiversity conservation are prime concerns for long-term conservation of rare and endangered species and their associated habitats in any ecological region. In this study, Gansu Province, a biodiversity hotspot, was chosen as the research area. We predicted the distribution patterns of suitable habitats for rare and endangered species. The replacement cost method was adopted to calculate the conservation value of rare and endangered species. The suitable habitat distribution area of rare and endangered wild animals reached 351,607.76 km2 (without overlapping area), while that of plants reached 72,988.12 km2 (without overlapping area). The conservation value of rare and endangered wildlife is US $1670.00 million. The high-value areas are mostly concentrated in the south and north of Gansu Province. The conservation value of rare and endangered wild plants is US $56,920.00 million. The high-value areas are mostly concentrated south of Gansu Province. The conservation value is US $58,590.00 million a year, and its distribution trend is gradually decreasing from northeast to southwest, with the highest in the forest area south of Gansu Province, followed by the Qilian Mountain area in the north. These results are of great significance for future improvement of the evaluation index system of ecosystem services and the development of ecosystem services and management strategies.

scholarly journals [First occurrence of Sea Lamprey, Petromyzon marinus Linnaeus 1758 (Agnatha, Petromyzontiformes, Petromyzontidae) in the River Mignone (Northern Lazio).]

2021 ◽  
Vol 8 (1) ◽  
pp. 29-34
Author(s):  
Vincenzo Ferri ◽  
Paolo Crescia ◽  
Christiana Soccini ◽  
Alessio Olini ◽  
Stefano Celletti
Keyword(s):  
Endangered Species ◽  
Petromyzon Marinus ◽  
Sea Lamprey ◽  
Environmental Context ◽  
Upstream Migration ◽  
Rare And Endangered Species ◽  
Fish Ladder ◽  
First Occurrence ◽  
Rare And Endangered

[The spring presence of two individuals of the Sea lamprey, Petromyzon marinus, in the River Mignone near Tarquinia (Northern Lazio) could highlight a new Italian reproductive site of this rare and endangered species. This exceptional possibility could certainly be favored by the good quality of both the waters of the Mignone, and the environmental context of the record, but would require the urgent equipment of the barrier of Le Mole with a fish ladder in order to allow the sea lamprey’s upstream migration towards the areas of the upper course, even more suitable for their reproduction].   [Article in Italian]

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