Impacts of pH on the Fitness and Immune System of Pacific White Shrimp

ABSTRACT This study was aimed to evaluate the dietary ambon lumut banana Musa cavendishii var. dwarf Paxton stem extract on the immune responses, growth, and survival rate of Pacific white shrimp against white spot disease. Ambon banana steam extract was obtained by maceration method using ethanol. The shrimps fed by pellet containing ambon banana stem extracts with different dosages for 29 days, i.e designated as 0.1 (A); 0.3 (B), and 0.5 (C) g/kg, the diet treatment without ambon banana stem extract without challenged test (K-), and diet treatment without ambon banana stem extract with challenged test (K+). Each treatment consisted of three replications. Feeding was conducted for 29 days of maintenance (four times a day). The results showed that the immune responses (average total hemocyte count: 45.15×106 cells/mL, phenoloxidase activity 1.03±0.08 OD, respiratory burst 0.95±0.04 OD, phagocytic activity 94.33±1.53%, growth (specific growth rate: 7.79±0.06%/day, feed ratio conversion was 52±0.01), and survival of treatment C (survival rate 100%) were higher compared with the treatment K+ (total hemocyte count: 3.83×106 cells/mL, phenoloxidase activity 0.04±0.01 OD, respiratory burst 0.18±0.06 OD, phagocytic activity 5.67±0.58%, specific growth rate: 2.61±0.08%/day, feed conversion ratio 2.11±0.02, survival rate: 50%). Therefore, banana stem extract at a dose of 0.5 g/kg everyday diet can be used to improve growth and nonspecific immune system against white spot disease on Pacific white shrimp. Keywords: Pacific white shrimp, white spot disease, immune response, Ambon banana stem extract ABSTRAK Penelitian ini bertujuan untuk menguji dosis optimal ekstrak batang pisang ambon melalui pakan dalam meningkatkan respon imun, pertumbuhan dan kelangsungan hidup udang putih terhadap penyakit white spot. Ekstrak batang pisang ambon lumut Musa cavendishii var. dwarf Paxton menggunakan metode maserasi dengan menggunakan pelarut etanol. Pakan udang yang mengandung ekstrak batang pisang ambon pada dosis yang berbeda selama 29 hari 0,1 (A); 0,3 (B), dan 0,5 (C) g/kg, pakan tanpa pemberian ekstrak batang pisang ambon dan tidak diuji tantang (K-), pakan tanpa pemberian ekstrak batang pisang ambon dan diuji tantang (K+). Masing-masing perlakuan terdiri atas tiga ulangan. Frekuensi pemberian pakan empat kali sehari selama 29 hari pemeliharaan. Hasil penelitian menunjukkan bahwa perlakuan C respons imun (rata-rata jumlah total hemosit: 45,15×106 sel/mL, aktivitas phenoloxydase 1,03±0,08 OD, respiratory burst 0,95±0,04 OD, aktivitas fagositik 94,33±1,53%, pertumbuhan (laju pertumbuhan spesifik: 7,79±0,06%/hari, rasio konversi pakan 0,52±0,01), dan kelangsungan hidup (survival rate 100%) lebih tinggi dibandingkan dengan perlakuan K+ (jumlah total hemosit: 3.83×106 sel/mL, aktivitas phenoloxydase 0,04±0,01 OD, respiratory burst 0,18±0,06 OD, aktivitas fagositik 5,67± 0,58%, laju pertumbuhan spesifik: 2,61±0,08%/hari, rasio konversi pakan 2,11±0,02, nilai kelangsungan hidup: 50%). Jadi, ekstrak batang pisang pada dosis 0,5 g/kg setiap hari pada pakan dapat digunakan untuk meningkatkan pertumbuhan dan sistem kekebalan tubuh nonspesifik terhadap penyakit bintik putih pada udang putih. Kata kunci: udang putih, white spot, respons imun, ekstrak batang pisang ambon

Download Full-text

Effects of spray-dried animal plasma on growth performance, survival, feed utilization, immune responses, and resistance to Vibrio parahaemolyticus infection of Pacific white shrimp (Litopenaeus vannamei)

PLoS ONE ◽

10.1371/journal.pone.0257792 ◽

2021 ◽

Vol 16 (9) ◽

pp. e0257792

Author(s):

Niti Chuchird ◽

Tirawat Rairat ◽

Arunothai Keetanon ◽

Putsucha Phansawat ◽

Chi-Chung Chou ◽

...

Keyword(s):

Immune Responses ◽

Growth Performance ◽

Litopenaeus Vannamei ◽

Vibrio Parahaemolyticus ◽

Positive Control ◽

Feed Utilization ◽

Pacific White Shrimp ◽

White Shrimp ◽

Total Hemocyte Count ◽

Spray Dried

Spray-dried animal plasma (SDP) in feed for several animal species provides health benefits, but research about use of SDP in shrimp feed is very limited. The objectives of the present study were to investigate the effects of dietary SDP on growth performance, feed utilization, immune responses, and prevention of Vibrio parahaemolyticus infection in Pacific white shrimp (Litopenaeus vannamei). In Experiment 1, the post-larvae were divided into five groups (four tank/group and 80 shrimp/tank) and fed four times daily diets with porcine SDP at 0, 1.5, 3, 4.5, and 6% of the diet for 45 days. In Experiment 2, the surviving shrimp from Experiment 1 were redistributed into six groups: four SDP groups as in Experiment 1 plus the positive and negative controls (four tank/group and 30 shrimp/tank). They were then challenged with V. parahaemolyticus by immersion at 105 colony-forming units (CFU)/mL and were fed with the same diets for another 4 days. In Experiment 1, shrimp fed 4.5% or 6% SDP diets had significantly higher body weight, survival rate, and improved feed conversion ratio. The immune parameters (total hemocyte count and phagocytic, phenoloxidase, and superoxide dismutase activities) of the shrimp fed 3–6% SDP diets also showed significant enhancement compared to the control. In Experiment 2, the survival rates of the 3–6% SDP groups were significantly higher than the positive control at day 4 after the immersion challenge. Likewise, the histopathological study revealed milder signs of bacterial infection in the hepatopancreas of the 3–6% SDP groups compared to the challenged positive control and 1.5% SDP groups. In conclusion, shrimp fed diets with SDP, especially at 4.5–6% of the diet, showed significant improvement in overall health conditions and better resistance to V. parahaemolyticus infection.

Download Full-text

Molecular effects of mannanase-hydrolyzed coprameal to intestinal immunity in the Pacific white shrimp (Litopenaeus vannamei)

10.1101/2021.02.16.431529 ◽

2021 ◽

Author(s):

Wanilada Rungrassamee ◽

Sopacha Arayamethakorn ◽

Nitsara Karoonuthaisiri ◽

Shih-Chu Chen ◽

Eric Chang ◽

...

Keyword(s):

Immune System ◽

Litopenaeus Vannamei ◽

Control Diet ◽

Pacific White Shrimp ◽

White Shrimp ◽

Control Group ◽

Intestinal Immunity ◽

Toll Like Receptor ◽

Transcript Levels ◽

Significant Difference

AbstractTo mitigate disease outbreak, an alternative approach through enhancing shrimp immunity was explored. Mannan oligosaccharides (MOS) have been previously reported to enhance shrimp immune system. Here, coprameal samples were digested with mannanase to yield MOS, namely, mannanase-hydrolyzed coprameal (MCM) and feasibility of MCM as shrimp immunostimulant in grow-out ponds was determined. Pacific white shrimp (Litopenaeus vannamei) were fed with the commercial diet containing 1% MCM as the MCM-supplemented group and compared to the non-MCM control diet. There was no significant difference in survival rates between the MCM-supplemented and the control groups throughout the 4-month-period of the trial (p > 0.05). Gene expression analysis in shrimp intestines revealed that the transcript levels of antimicrobial peptides (anti-lipopolysaccharide factor isoform 1 (alf1), penaeidin (pen3a) and crustin (crus)) and lysozymes (lyz) were not significantly different in the MCM-supplemented group. Meanwhile, C-type lectin and toll-like receptor transcript levels, whose gene products play roles as pattern recognition proteins, were significantly higher in a group fed with MCM for 2- and 4-month periods than those of the control group (p < 0.05). The increased transcript levels of C-type lectin and toll-like receptor provide evidence for potential implementation of MCM as feed supplement to modulate shrimp immune system.

Download Full-text

Dietary ambon lumut banana stem extract Musa cavendishii var. dwarf Paxton as an immunostimulant for white spot disease prevention in Pacific white shrimp Litopenaeus vannamei

Jurnal Akuakultur Indonesia ◽

10.19027/jai.16.2.164-173 ◽

2017 ◽

Vol 16 (2) ◽

pp. 164

Author(s):

Afriani Ramadhan ◽

Sri Nuryati ◽

Nur Bambang Priyoutomo ◽

Alimuddin Alimuddin

Keyword(s):

Survival Rate ◽

Respiratory Burst ◽

Pacific White Shrimp ◽

White Spot ◽

White Shrimp ◽

White Spot Disease ◽

Spot Disease ◽

Total Hemocyte Count ◽

Stem Extract ◽

Banana Stem

ABSTRACT This study was aimed to evaluate the dietary ambon lumut banana Musa cavendishii var. dwarf Paxton stem extract on the immune responses, growth, and survival rate of Pacific white shrimp against white spot disease. Ambon banana steam extract was obtained by maceration method using ethanol. The shrimps fed by pellet containing ambon banana stem extracts with different dosages for 29 days, i.e designated as 0.1 (A); 0.3 (B), and 0.5 (C) g/kg, the diet treatment without ambon banana stem extract without challenged test (K-), and diet treatment without ambon banana stem extract with challenged test (K+). Each treatment consisted of three replications. Feeding was conducted for 29 days of maintenance (four times a day). The results showed that the immune responses (average total hemocyte count: 45.15×106 cells/mL, phenoloxidase activity 1.03±0.08 OD, respiratory burst 0.95±0.04 OD, phagocytic activity 94.33±1.53%, growth (specific growth rate: 7.79±0.06%/day, feed ratio conversion was 52±0.01), and survival of treatment C (survival rate 100%) were higher compared with the treatment K+ (total hemocyte count: 3.83×106 cells/mL, phenoloxidase activity 0.04±0.01 OD, respiratory burst 0.18±0.06 OD, phagocytic activity 5.67±0.58%, specific growth rate: 2.61±0.08%/day, feed conversion ratio 2.11±0.02, survival rate: 50%). Therefore, banana stem extract at a dose of 0.5 g/kg everyday diet can be used to improve growth and nonspecific immune system against white spot disease on Pacific white shrimp. Keywords: Pacific white shrimp, white spot disease, immune response, Ambon banana stem extract ABSTRAK Penelitian ini bertujuan untuk menguji dosis optimal ekstrak batang pisang ambon melalui pakan dalam meningkatkan respon imun, pertumbuhan dan kelangsungan hidup udang putih terhadap penyakit white spot. Ekstrak batang pisang ambon lumut Musa cavendishii var. dwarf Paxton menggunakan metode maserasi dengan menggunakan pelarut etanol. Pakan udang yang mengandung ekstrak batang pisang ambon pada dosis yang berbeda selama 29 hari 0,1 (A); 0,3 (B), dan 0,5 (C) g/kg, pakan tanpa pemberian ekstrak batang pisang ambon dan tidak diuji tantang (K-), pakan tanpa pemberian ekstrak batang pisang ambon dan diuji tantang (K+). Masing-masing perlakuan terdiri atas tiga ulangan. Frekuensi pemberian pakan empat kali sehari selama 29 hari pemeliharaan. Hasil penelitian menunjukkan bahwa perlakuan C respons imun (rata-rata jumlah total hemosit: 45,15×106 sel/mL, aktivitas phenoloxydase 1,03±0,08 OD, respiratory burst 0,95±0,04 OD, aktivitas fagositik 94,33±1,53%, pertumbuhan (laju pertumbuhan spesifik: 7,79±0,06%/hari, rasio konversi pakan 0,52±0,01), dan kelangsungan hidup (survival rate 100%) lebih tinggi dibandingkan dengan perlakuan K+ (jumlah total hemosit: 3.83×106 sel/mL, aktivitas phenoloxydase 0,04±0,01 OD, respiratory burst 0,18±0,06 OD, aktivitas fagositik 5,67± 0,58%, laju pertumbuhan spesifik: 2,61±0,08%/hari, rasio konversi pakan 2,11±0,02, nilai kelangsungan hidup: 50%). Jadi, ekstrak batang pisang pada dosis 0,5 g/kg setiap hari pada pakan dapat digunakan untuk meningkatkan pertumbuhan dan sistem kekebalan tubuh nonspesifik terhadap penyakit bintik putih pada udang putih. Kata kunci: udang putih, white spot, respons imun, ekstrak batang pisang ambon

Download Full-text

Extract of seaweed Gracilaria verrucosa as immunostimulant to controlling white spot disease in Pacific white shrimp Litopenaeus vannamei

Jurnal Akuakultur Indonesia ◽

10.19027/jai.16.2.174-183 ◽

2017 ◽

Vol 16 (2) ◽

pp. 174 ◽

Cited By ~ 1

Author(s):

Aminatul Zahra ◽

Sukenda Sukenda ◽

Dinamella Wahjuningrum

Keyword(s):

Immune Response ◽

Respiratory Burst ◽

White Spot Syndrome Virus ◽

Pacific White Shrimp ◽

Gracilaria Verrucosa ◽

White Spot ◽

White Shrimp ◽

Percent Survival ◽

Total Hemocyte Count ◽

White Spot Syndrome

ABSTRACT This experiment was conducted to examine effect of Gracilaria verrucosa extract in diet with different dosages to enhance immune response and resistance against white spot syndrome virus (WSSV) in the Pacific white shrimp. The experiment consisted of six treatments in three replicates respectively, namely K- (without extract), K + (without extract + infected WSSV), A (2 g/kg of feed + infected WSSV), B (3 g/kg of feed + infected WSSV), C (4 g/kg of feed + infected WSSV), and D (5 g/kg of feed + infected WSSV). White shrimp with initial body weight of 6.07±0.10 g were reared in the (60×30×30) cm with density of 10 shrimps/aquarium. G. verrucosa was extracted with ethyl acetate. Pacific white shrimp had been fed medicated feed three times daily 3% at satiation for 14 days. At 15th days, white shrimp were challenged with WSSV at 0.1 mL/shrimp intramuscularly. The results showed that the immune response shrimp (total hemocyte count, phagocytic activity, respiratory burst, and phenoloxidase activity) fed medicated feed increased significantly compared to positive and negative controls. The best relative percent survival post-challenge test was at 4 g/kg dose of G. verrucosa, i.e 41.07±3.09%. Confirmation of WSSV using PCR showed that shrimps (A, B, C, D, and K+) were positively infected by WSSV. It was concluded that 4 g/kg dose of G. verrucosa gave the best result to enhance immune response and resistance to WSSV infection. Keywords: seaweed, Gracilaria verrucosa, immunostimulant, Pacific white shrimp, WSSV ABSTRAK Penelitian ini bertujuan untuk menguji pengaruh pemberian ekstrak Gracilaria verrucosa melalui pakan dengan dosis yang berbeda untuk meningkatkan imunitas dan resistensi udang vaname terhadap serangan white spot syndrome virus (WSSV). Penelitian ini terdiri atas enam perlakuan dan masing-masing tiga ulangan, yaitu K- (tanpa ekstrak), K+ (tanpa ekstrak + infeksi WSSV), A (2 g/kg pakan + infeksi WSSV), B (3 g/kg pakan + infeksi WSSV), C (4 g/kg pakan + infeksi WSSV), dan D (5 g/kg pakan + infeksi WSSV). Udang vaname dengan bobot 6,07±0,10 g dipelihara dalam akuarium dengan ukuran (60×30×30) cm dengan padat tebar 10 ekor/akuarium. Udang diberi pakan perlakuan secara at satiation sebanyak tiga kali sehari selama 14 hari. Pada hari ke-15 diuji tantang dengan WSSV pada dosis 0,1 mL/ekor secara intramuskular. Hasil penelitian menunjukkan bahwa respons imun udang (total hemosit, aktivitas fagositik, respiratory burst, dan aktivitas fenoloksidase) yang diberi pakan mengandung ekstrak G. verrucosa mengalami peningkatan signifikan dibanding perlakuan kontrol positif maupun negatif. Relative percent survival terbaik pasca uji tantang pada perlakuan C (4 g/kg), yaitu 41,07±3,09%. Konfirmasi WSSV dengan menggunakan PCR menunjukkan hasil bahwa udang (A, B, C, D, and K+) positif terinfeksi WSSV. Disimpulkan bahwa dosis ekstrak G. verrucosa 4 g/kg pakan memberikan hasil terbaik untuk meningkatkan respons imun pada udang vaname dan resistensi terhadap WSSV. Kata kunci: rumput laut, Gracilaria verrucosa, imunostimulan, udang vaname, WSSV

Download Full-text

Extract of seaweed Gracilaria verrucosa as immunostimulant to controlling white spot disease in Pacific white shrimp Litopenaeus vannamei

Jurnal Akuakultur Indonesia ◽

10.19027/jai.16.2.185-194 ◽

2017 ◽

Vol 16 (2) ◽

pp. 185

Author(s):

Aminatul Zahra ◽

Sukenda Sukenda ◽

Dinamella Wahjuningrum

Keyword(s):

Immune Response ◽

Respiratory Burst ◽

White Spot Syndrome Virus ◽

Pacific White Shrimp ◽

Gracilaria Verrucosa ◽

White Spot ◽

White Shrimp ◽

Percent Survival ◽

Total Hemocyte Count ◽

White Spot Syndrome

ABSTRACT This experiment was conducted to examine effect of Gracilaria verrucosa extract in diet with different dosages to enhance immune response and resistance against white spot syndrome virus (WSSV) in the Pacific white shrimp. The experiment consisted of six treatments in three replicates respectively, namely K- (without extract), K + (without extract + infected WSSV), A (2 g/kg of feed + infected WSSV), B (3 g/kg of feed + infected WSSV), C (4 g/kg of feed + infected WSSV), and D (5 g/kg of feed + infected WSSV). White shrimp with initial body weight of 6.07±0.10 g were reared in the (60×30×30) cm with density of 10 shrimps/aquarium. G. verrucosa was extracted with ethyl acetate. Pacific white shrimp had been fed medicated feed three times daily 3% at satiation for 14 days. At 15th days, white shrimp were challenged with WSSV at 0.1 mL/shrimp intramuscularly. The results showed that the immune response shrimp (total hemocyte count, phagocytic activity, respiratory burst, and phenoloxidase activity) fed medicated feed increased significantly compared to positive and negative controls. The best relative percent survival post-challenge test was at 4 g/kg dose of G. verrucosa, i.e 41.07±3.09%. Confirmation of WSSV using PCR showed that shrimps (A, B, C, D, and K+) were positively infected by WSSV. It was concluded that 4 g/kg dose of G. verrucosa gave the best result to enhance immune response and resistance to WSSV infection. Keywords: seaweed, Gracilaria verrucosa, immunostimulant, Pacific white shrimp, WSSV ABSTRAK Penelitian ini bertujuan untuk menguji pengaruh pemberian ekstrak Gracilaria verrucosa melalui pakan dengan dosis yang berbeda untuk meningkatkan imunitas dan resistensi udang vaname terhadap serangan white spot syndrome virus (WSSV). Penelitian ini terdiri atas enam perlakuan dan masing-masing tiga ulangan, yaitu K- (tanpa ekstrak), K+ (tanpa ekstrak + infeksi WSSV), A (2 g/kg pakan + infeksi WSSV), B (3 g/kg pakan + infeksi WSSV), C (4 g/kg pakan + infeksi WSSV), dan D (5 g/kg pakan + infeksi WSSV). Udang vaname dengan bobot 6,07±0,10 g dipelihara dalam akuarium dengan ukuran (60×30×30) cm dengan padat tebar 10 ekor/akuarium. Udang diberi pakan perlakuan secara at satiation sebanyak tiga kali sehari selama 14 hari. Pada hari ke-15 diuji tantang dengan WSSV pada dosis 0,1 mL/ekor secara intramuskular. Hasil penelitian menunjukkan bahwa respons imun udang (total hemosit, aktivitas fagositik, respiratory burst, dan aktivitas fenoloksidase) yang diberi pakan mengandung ekstrak G. verrucosa mengalami peningkatan signifikan dibanding perlakuan kontrol positif maupun negatif. Relative percent survival terbaik pasca uji tantang pada perlakuan C (4 g/kg), yaitu 41,07±3,09%. Konfirmasi WSSV dengan menggunakan PCR menunjukkan hasil bahwa udang (A, B, C, D, and K+) positif terinfeksi WSSV. Disimpulkan bahwa dosis ekstrak G. verrucosa 4 g/kg pakan memberikan hasil terbaik untuk meningkatkan respons imun pada udang vaname dan resistensi terhadap WSSV. Kata kunci: rumput laut, Gracilaria verrucosa, imunostimulan, udang vaname, WSSV

Download Full-text