scholarly journals Mangrove Facies Drives Resistance and Resilience of Sediment Microbes Exposed to Anthropic Disturbance

2019 ◽  
Vol 9 ◽  
Author(s):  
Cécile Capdeville ◽  
Thomas Pommier ◽  
Jonathan Gervaix ◽  
François Fromard ◽  
Jean-Luc Rols ◽  
...  
Keyword(s):  
2017 ◽  
Vol 599-600 ◽  
pp. 1479-1484 ◽  
Author(s):  
Songlin Liu ◽  
Zhijian Jiang ◽  
Jingping Zhang ◽  
Yunchao Wu ◽  
Xiaoping Huang ◽  
...  

2019 ◽  
Author(s):  
Graham A. Colby ◽  
Matti O. Ruuskanen ◽  
Kyra A. St. Pierre ◽  
Vincent L. St. Louis ◽  
Alexandre J. Poulain ◽  
...  

AbstractTemperatures in the Arctic are expected to increase dramatically over the next century, yet little is known about how microbial communities and their underlying metabolic processes will be affected by these environmental changes in freshwater sedimentary systems. To address this knowledge gap, we analyzed sediments from Lake Hazen, NU Canada. Here, we exploit the spatial heterogeneity created by varying runoff regimes across the watershed of this uniquely large lake at these latitudes to test how a transition from low to high runoff, used as one proxy for climate change, affects the community structure and functional potential of dominant microbes. Based on metagenomic analyses of lake sediments along these spatial gradients, we show that increasing runoff leads to a decrease in taxonomic and functional diversity of sediment microbes. Our findings are likely to apply to other, smaller, glacierized watersheds typical of polar or high latitude / high altitudes ecosystems; we can predict that such changes will have far reaching consequences on these ecosystems by affecting nutrient biogeochemical cycling, the direction and magnitude of which are yet to be determined.


2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Neelam M. Nathani ◽  
Kaushambee J. Dave ◽  
Priyanka P. Vatsa ◽  
Mayur S. Mahajan ◽  
Parth Sharma ◽  
...  

AbstractProkaryoplankton genomes from the deep marine sediments are less explored compared to shallow shore sediments. The Gulfs of Kathiawar peninsula experience varied currents and inputs from different on-shore activities. Any perturbations would directly influence the microbiome and their normal homeostasis. Advancements in reconstructing genomes from metagenomes allows us to understand the role of individual unculturable microbes in ecological niches like the Gulf sediments. Here, we report 309 bacterial and archaeal genomes assembled from metagenomics data of deep sediments from sites in the Gulf of Khambhat and Gulf of Kutch as well as a sample from the Arabian Sea. Phylogenomics classified them into 5 archaeal and 18 bacterial phyla. The genomes will facilitate understanding of the physiology, adaptation and impact of on-shore anthropogenic activities on the deep sediment microbes.


2017 ◽  
Vol 5 (2) ◽  
pp. 109-112
Author(s):  
Noratikah Kamal Baharin ◽  
Noorkhalilie Che Abd Aziz ◽  
Faisal Mohamad ◽  
Suganthi Appalasamy

Extraction of pure genomic DNA from microbes isolated from river sediments often hindered byco-isolated carryovers from the reagents used which reduces the yield and quality of DNA.Existing methods were lengthy, expensive and does not result in high yield of genomic DNA inshort time. This study describes a rapid and inexpensive DNA extraction method that involvedminimal purification step without phenol or other carcinogenic reagents. The microbial DNAextraction steps also does not require the use of special laboratory equipment. This method usesCetyltrimethylammonium bromide (CTAB) followed by ethanol based purification steps andwithin one hour the extraction of microbial DNA could be completed. The extracted genomicDNA from microbes isolated from river sediment of Sungai Kelantan, Malaysia using the methoddescribed in this study is of quality suitable for other downstream researches such asmetagenomics sequencing.


Archaea ◽  
2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Yantian Ma ◽  
Fangpeng Liu ◽  
Zhaoyu Kong ◽  
Jianhua Yin ◽  
Wenbo Kou ◽  
...  

Archaea plays an important role in the global geobiochemical circulation of various environments. However, much less is known about the ecological role of archaea in freshwater lake sediments. Thus, investigating the structure and diversity of archaea community is vital to understand the metabolic processes in freshwater lake ecosystems. In this study, sediment physicochemical properties were combined with the results from 16S rRNA clone library-sequencing to examine the sediment archaea diversity and the environmental factors driving the sediment archaea community structures. Seven sites were chosen from Poyang Lake, including two sites from the main lake body and five sites from the inflow river estuaries. Our results revealed high diverse archaea community in the sediment of Poyang Lake, including Bathyarchaeota (45.5%), Euryarchaeota (43.1%), Woesearchaeota (3.6%), Pacearchaeota (1.7%), Thaumarchaeota (1.4%), suspended Lokiarchaeota (0.7%), Aigarchaeota (0.2%), and Unclassified Archaea (3.8%). The archaea community compositions differed among sites, and sediment property had considerable influence on archaea community structures and distribution, especially total organic carbon (TOC) and metal lead (Pb) (p<0.05). This study provides primary profile of sediment archaea distribution in freshwater lakes and helps to deepen our understanding of lake sediment microbes.


2003 ◽  
Vol 46 (1) ◽  
pp. 55-61 ◽  
Author(s):  
J. E. Johnson ◽  
R. T. Hill
Keyword(s):  
Deep Sea ◽  

Water ◽  
2022 ◽  
Vol 14 (2) ◽  
pp. 251
Author(s):  
Subhomita Ghosh Ghosh Roy ◽  
Charles F. Wimpee ◽  
S. Andrew McGuire ◽  
Timothy J. Ehlinger

Urbanization results in higher stormwater loadings of pollutants such as metals and nutrients into surface waters. This directly impacts organisms in aquatic ecosystems, including microbes. Sediment microbes are known for pollution reduction in the face of contamination, making bacterial communities an important area for bioindicator research. This study explores the pattern of bacterial responses to metal and nutrient pollution loading and seeks to evaluate whether bacterial indicators can be effective as a biomonitoring risk assessment tool for wetland ecosystems. Microcosms were built containing sediments collected from wetlands in the urbanizing Pike River watershed in southeastern Wisconsin, USA, with metals and nutrients added at 7 day intervals. Bacterial DNA was extracted from the microcosm sediments, and taxonomical profiles of bacterial communities were identified up to the genera level by sequencing 16S bacterial rRNA gene (V3–V4 region). Reduction of metals (example: 90% for Pb) and nutrients (example: 98% for NO3−) added in water were observed. The study found correlations between diversity indices of genera with metal and nutrient pollution as well as identified specific genera (including Fusibacter, Aeromonas, Arthrobacter, Bacillus, Bdellovibrio, and Chlorobium) as predictive bioindicators for ecological risk assessment for metal pollution.


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