Urine Sediment Microbes as Part of Reproductive Status: Can PCR be Made More Portable?

2010 ◽  
Vol 83 (Suppl_1) ◽  
pp. 345-345
Author(s):  
Keith Ferguson ◽  
Kenneth L. Campbell
2020 ◽  
Vol 46 (4) ◽  
pp. 382-394
Author(s):  
Holli C. Eskelinen ◽  
Jill L. Richardson ◽  
Juliana K. Wendt

2020 ◽  
Vol 15 (3) ◽  
pp. 237-248
Author(s):  
Valeria Senigaglia ◽  
Lars Bejder

Marine wildlife tourism attractions often use food rewards to ensure close-up encounters with freeranging animals. In Bunbury, Western Australia, the Dolphin Discovery Centre (DDC) conducts a foodprovision program where bottlenose dolphins (N = 22; between 2000 and 2018) are offered food rewards to encourage their visitation at a beach in front of the DDC. We used historical records on individual beach visits by adult female dolphins collected by the DDC from 2000 to 2018 to develop generalized mixed effects models (GLMM) to test whether the frequency of beach visitation was influenced by their reproductive status (pregnant, lactating, nonreproductive) or climatic events (El Niño-Southern Oscillation phases) that could affect prey availability. We also quantified the behavioral budget of dolphins during food-provisioning sessions and documented intra- and interspecific aggressive behaviors using individual focal follows collected in 2017–2018. Provisioned females spend most of the time resting within the interaction area (66.3%) and aggressive interactions arise as a consequence of dominance behavior over food access. Visitation rates were most influenced by reproductive status with pregnant and lactating females visiting the provisioning area more frequently (z = 2.085, p = 0.037 and z = 2.437, p = 0.014, respectively). Females that frequently visit the provisioning area expose their dependent calves to regular human interactions at an early age when they are more susceptible to behavioral conditioning. Such experiences could cause the loss of awareness towards humans and promote maladaptive behaviors such as begging that increase risk of entanglement in fishing gear, boat strikes, and propeller injuries.


2021 ◽  
Vol 132 ◽  
pp. S275
Author(s):  
Alison Muir ◽  
Juliana Troiani ◽  
Alicia Mosley ◽  
Zhiqian Fan ◽  
Matthew Dulik ◽  
...  

2020 ◽  
Vol 58 (10) ◽  
pp. 1759-1767
Author(s):  
Mieke Steenbeke ◽  
Sander De Bruyne ◽  
Jerina Boelens ◽  
Matthijs Oyaert ◽  
Griet Glorieux ◽  
...  

AbstractObjectivesIn this study, the possibilities of Fourier-transformed infrared spectroscopy (FTIR) for analysis of urine sediments and for detection of bacteria causing urinary tract infections (UTIs) were investigated.MethodsDried urine specimens of control subjects and patients presenting with various nephrological and urological conditions were analysed using mid-infrared spectroscopy (4,000–400 cm−1). Urine samples from patients with a UTI were inoculated on a blood agar plate. After drying of the pure bacterial colonies, FTIR was applied and compared with the results obtained by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Chemometric data analysis was used to classify the different species.ResultsDue to the typical molecular assignments of lipids, proteins, nucleic acids and carbohydrates, FTIR was able to identify bacteria and showed promising results in the detection of proteins, lipids, white and red blood cells, as well as in the identification of crystals. Principal component analysis (PCA) allowed to differentiate between Gram-negative and Gram-positive species and soft independent modelling of class analogy (SIMCA) revealed promising classification ratios between the different pathogens.ConclusionsFTIR can be considered as a supplementary method for urine sediment examination and for detection of pathogenic bacteria in UTI.


2007 ◽  
Vol 384 (1-2) ◽  
pp. 28-34 ◽  
Author(s):  
Tzu-I Chien ◽  
Jau-Tsuen Kao ◽  
Hui-Lan Liu ◽  
Po-Chang Lin ◽  
Jhih-Sian Hong ◽  
...  
Keyword(s):  

1977 ◽  
Vol 70 (4) ◽  
pp. 588-590 ◽  
Author(s):  
F. S. Guillot ◽  
J. R. Coppedge ◽  
J. L. Goodenough ◽  
T. S. Adam ◽  
E. Ahrens
Keyword(s):  

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