scholarly journals New Method for Isolation of DNA from River Sediment Microbes of Sungai Kelantan, Malaysia

2017 ◽  
Vol 5 (2) ◽  
pp. 109-112
Author(s):  
Noratikah Kamal Baharin ◽  
Noorkhalilie Che Abd Aziz ◽  
Faisal Mohamad ◽  
Suganthi Appalasamy
Keyword(s):  
Genomic Dna ◽  
River Sediment ◽  
River Sediments ◽  
High Yield ◽  
Laboratory Equipment ◽  
Yield And Quality ◽  
Dna Extraction Method ◽  
Microbial Dna ◽  
Sediment Microbes

Extraction of pure genomic DNA from microbes isolated from river sediments often hindered byco-isolated carryovers from the reagents used which reduces the yield and quality of DNA.Existing methods were lengthy, expensive and does not result in high yield of genomic DNA inshort time. This study describes a rapid and inexpensive DNA extraction method that involvedminimal purification step without phenol or other carcinogenic reagents. The microbial DNAextraction steps also does not require the use of special laboratory equipment. This method usesCetyltrimethylammonium bromide (CTAB) followed by ethanol based purification steps andwithin one hour the extraction of microbial DNA could be completed. The extracted genomicDNA from microbes isolated from river sediment of Sungai Kelantan, Malaysia using the methoddescribed in this study is of quality suitable for other downstream researches such asmetagenomics sequencing.

High Microsatellite and SNP Genotyping Success Rates Established in a Large Number of Genomic DNA Samples Extracted From Mouth Swabs and Genotypes

2006 ◽  
Vol 9 (4) ◽  
pp. 501-506 ◽  
Author(s):  
Josine L. Min ◽  
Nico Lakenberg ◽  
Margreet Bakker-Verweij ◽  
Eka Suchiman ◽  
Dorret I. Boomsma ◽  
...  
Keyword(s):  
Success Rate ◽  
Genomic Dna ◽  
Snp Genotyping ◽  
High Yield ◽  
Success Rates ◽  
Nucleotide Polymorphism ◽  
High Quality ◽  
Single Nucleotide ◽  
Dna Yield ◽  
Yield And Quality

AbstractIn this article, we present the genomic DNA yield and the microsatellite and single nucleotide polymorphism (SNP) genotyping success rates of genomic DNA extracted from a large number of mouth swab samples. In total, the median yield and quality was determined in 714 individuals and the success rates in 378,480 genotypings of 915 individuals. The median yield of genomic DNA per mouth swab was 4.1 μg (range 0.1–42.2 μg) and was not reduced when mouth swabs were stored for at least 21 months prior to extraction. A maximum of 20 mouth swabs is collected per participant. Mouth swab samples showed in, respectively, 89% for 390 microsatellites and 99% for 24 SNPs a genotyping success rate higher than 75%. A very low success rate of genotyping (0%–10%) was obtained for 3.2% of the 915 mouth swab samples using microsatellite markers. Only 0.005% of the mouth swab samples showed a geno-typing success rate lower than 75% (range 58%–71%) using SNPs. Our results show that mouth swabs can be easily collected, stored by our conditions for months prior to DNA extraction and result in high yield and high-quality DNA appropriate for genotyping with high success rate including whole genome searches using microsatellites or SNPs.

scholarly journals Stomatal Conductance and Chlorophyll Characteristics and Their Relationship with Yield of Some Cocoa Clones Under Tectona grandis, Leucaena sp., and Cassia surattensis.

2015 ◽  
Vol 31 (2) ◽  
pp. 99-108
Author(s):  
Fakhrusy Zakariyya ◽  
Adi Prawoto
Keyword(s):  
Stomatal Conductance ◽  
Chlorophyll Content ◽  
Tectona Grandis ◽  
High Yield ◽  
Climate Type ◽  
Pod Yield ◽  
Yield And Quality ◽  
Diffusive Resistance ◽  
Cocoa Production

An optimum physiological condition will support high yield and quality of cocoa production. The research was aimed to study the effects of stomatal conductance and chlorophyll content related to cocoa production under three shade regimes.This research was conducted in Kaliwining Experimental Station, elevation of 45 m above sea level with D climate type based on Schmidt & Fergusson. Cocoa trees which were planted in 1994 at a spacing of 3 X 3 m were used in the study planted by using split plot design. The shade tree species were teak (Tectona grandis), krete (Cassiasurattensis), and lamtoro (Leucaena sp.) as the main plots, and cocoa clones of Sulawesi 01,Sulawesi 02, KKM 22 and KW 165 as sub plots. This study showed that there was interaction between cocoa clone and shade species for stomatal conductance where stomatal diffusive resistance of KKM 22 was the best under Leucaena sp.and Cassiasurattensis with the values of 1.38 and 1.34 s.cm -1, respectively. The highest chlorophyll content, stomatal index and transpiration values was under Leucaena sp. shade. There was positive correlation between chlorophyll content and transpiration with pod yield of cocoa. The highest yield and the lowest bean count wereobtainedon Sulawesi 01 clone under Leucaenasp. shade.Keywords: stomatal conductance, transpiration, diffusive resistance, shades trees, clones,pod yield

scholarly journals EFFECT OF BIOFERTILIZER AND COMPOST ON NITRATE STATUS, YIELD AND QUALITY OF POTATO TUBER UNDER NEWLY RECLAIMED SANDY SOIL

AGROFOR ◽  
2018 ◽  
Vol 3 (1) ◽  
Author(s):  
S.F. EL-SAYED ◽  
A.A. GAHRIB ◽  
Rasha R. EID
Keyword(s):  
Sandy Soil ◽  
Control Treatment ◽  
High Yield ◽  
Potato Tubers ◽  
Treatment Control ◽  
Mineral Fertilization ◽  
Marketable Yield ◽  
Yield And Quality ◽  
Compost Treatment

This investigation was carried out during the two summer seasons of 2015 and2016 in sandy soil on potato culitvar "Sante" to study the effect of using 100%compost (15 t/fed.) and 50% compost + nitrogen fixing bacteria (Azotobacter, andPseudomonas alone or together) on potato yield and quality as compared to theconventional mineral fertilization (120-75-150 kg/fed. NPK + 5 toncompost/fed.(control)). No significant differences in tubers yield/fed. were detectedbetween mineral fertilization (control) and using 100% compost (15t/fed).However, control treatment significantly produced a high yield per feddan,more than using 50% compost + any biofertilizer treatment.Using composttreatment at 15 t/fed.execeed all biofertilizer treatments in marketable yield in bothseasons, but without significant differences as compared with mineral fertilization(control).No significant differences in tuber dray matter and content of starch intuber were found between using compost treatment at 15 ton/fed. and mineralfertilization treatment (control)in both seasons. Nevertheless, application of 50%compost+ 4 applications of Azotobacter and Pseudomonas had the highest tuberconcentrations of starch and nitrogen with significant differences as compared withthe mineral fertilization.Using50% compost + 4 applications of Azotobacter orPseudomonas or both (Azotobacter + Pseudomonas )and application of 100%compost caused producing potato tubers with the lowest concentration of nitratewith significant differences as compared with the mineral fertilization. Nosignificant differences were detected between mineral and organic fertilizersconcerning P and K concentrations in tubers.

scholarly journals Determination of the Effect of Humic Acid on Growth and Development Parameters of Parsley (Petroselinum sativum Hoffm.) Grown in Boron Soil

2018 ◽  
Vol 47 (1) ◽  
pp. 183-193 ◽  
Author(s):  
Tuncay TURSUN ◽  
Sener AKINCI ◽  
Esin BOZKURT
Keyword(s):  
Humic Acid ◽  
Growth Parameters ◽  
Dry Weight ◽  
High Yield ◽  
Yield And Quality ◽  
Icp Ms ◽  
Growth Analyses ◽  
Nutrient Solutions

Boron is an important micronutrient, required for all plant growth, and critical for high yield and quality of crops. The aim of the present research was to determine the effects of boron on pot-grown parsley (Petroselinum sativum Hoffm.). The experimental design consisted of four treatments using Hoagland-Arnon (1950) nutrient solutions with two different boron concentrations (B1 - 15 ppm and B2 - 150 ppm), each with and without 10 ml humic acid addition (HB1 and HB2), and controls with full strength Hoagland-Arnon solutions. Growth analyses of the parsley revealed that 15 ppm boron application caused an increase in root length leaf fresh and dry weight root fresh and dry weight and leaf area compared to control values. 150 ppm B (B2) concentration decreased all growth parameters compared to controls. The two humic acid treatments (HB1 and HB2) did not increase any of those growth parameters either in controls (C) or in the two boron (B1 and B2) concentrations. Analysis by (ICP-MS) revealed that B content in the leaves increased gradually in B1 and B2, as well as in both humic treatments where in HB2 it increased to 99.38% compared to B1. In the leaves, Mn, Zn and Fe contents behaved the same as B, increasing in all treatments, with the amounts in HB2 being significantly greater than in C, B1 and B2 leaves.

Phytoestrogen-containing diets offer benefits for mouse embryology but lead to fewer offspring being produced

2020 ◽  
Vol 54 (6) ◽  
pp. 536-545
Author(s):  
Jussi Helppi ◽  
Ronald Naumann ◽  
Oliver Zierau
Keyword(s):  
Transgenic Mice ◽  
Diet Group ◽  
High Yield ◽  
Standard Diet ◽  
Potential Health ◽  
Protein Sources ◽  
Yield And Quality ◽  
Experimental Mouse ◽  
Standard Diet Group

One of the most commonly used protein sources in rodent diets is soy, which is naturally rich in phytoestrogens. Although phytoestrogens have shown potential health benefits in humans, they may also have the ability to disrupt reproduction. Consequently, there has been a tendency to try to exclude them from rodent diets. In the current study, we investigated whether phytoestrogen content in the mouse diet could affect reproduction in mice used as embryo donors. Donor mice (C57BL/6JOlaHsd) were maintained with three different diets: high phytoestrogen (ca. 400 mg/kg genistein), low phytoestrogen (ca. 10 mg/kg genistein) and standard breeding diet (ca. 120 mg/kg genistein). Mice fed a high phytoestrogen diet had a high yield of plugs, embryos, and injectable embryos, as well as producing good quality embryos. Results from donor mice fed a low phytoestrogen diet were consistently but only slightly inferior, whereas mice fed a standard diet performed the poorest. Interestingly, the largest number of born and weaned offspring were observed when recipient females received embryos from the standard diet group. Sperm yield and quality of stud males did not differ between the groups. We surmize that for experimental endpoints requiring fertilized embryos it may be more beneficial to feed mice a diet containing phytoestrogen, but if the goal is to produce transgenic mice, a diet high in phytoestrogen may be inadvisable. In conclusion, care should be taken when selecting a diet for experimental mouse colonies as phytoestrogen could influence the study outcome.

scholarly journals Transcriptome and genome sequencing elucidates the molecular basis for the high yield and good quality of the hybrid rice variety Chuanyou6203

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Juansheng Ren ◽  
Fan Zhang ◽  
Fangyuan Gao ◽  
Lihua Zeng ◽  
Xianjun Lu ◽  
...  
Keyword(s):  
Molecular Mechanism ◽  
Hybrid Rice ◽  
Rice Variety ◽  
Utilization Efficiency ◽  
High Yield ◽  
Mrna Editing ◽  
High Quality ◽  
Flag Leaves ◽  
Yield And Quality

AbstractThe yield heterosis of rice is sought by farmers and strong contributes to food safety, but the quality of hybrid rice may be reduced. Therefore, developing new varieties with both high yield and good quality is a heavily researched topic in hybrid rice breeding. However, the molecular mechanism governing yield heterosis and high rice quality has not been elucidated to date. In this study, a comparative transcriptomics and genomic analysis was performed on a hybrid rice variety, Chuanyou6203 (CY6203), and its parents to investigate the molecular mechanism and gene regulation network governing the formation of yield and quality stages. A total of 66,319 SNPs and InDels between CH3203 and C106B were detected in the 5′-UTR, exon, intronic, and 3′-UTR regions according to the reference genome annotation, which involved 7473 genes. A total of 436, 70, 551, 993, and 1216 common DEGs between CY6203 and both of its parents were identified at the same stage in panicles and flag leaves. Of the common DEGs, the numbers of upregulated DEGs between CY6203 and CH3203 were all greater than those of upregulated DEGs between CY6203 and C106B in panicles and flag leaves at the booting, flowering, and middle filling stages. Approximately 40.61% of mRNA editing ratios were between 0.4 and 0.6, and 1.68% of mRNA editing events (editing ratio ≥ 0.8) in CY6203 favored one of its parents at three stages or a particular stage, suggesting that the hypothetical heterosis mechanism of CY6203 might involve dominance or epistasis. Also 15,934 DEGs were classified into 19 distinct modules that were classified into three groups by the weighted gene coexpression network analysis. Through transcriptome analysis of panicles and flag leaves in the yield and quality stages, the DEGs in the green-yellow module primarily contributed to the increase in the source of CY6203 due to an in increase in photosynthetic efficiency and nitrogen utilization efficiency, and a small number of DEGs related to the grain number added spikelet number per panicle amplified its sink. The balanced expression of the major high-quality alleles of C106B and CH3203 in CY6203 contributed to the outstanding quality of CY6203. Our transcriptome and genome analyses offer a new data set that may help to elucidate the molecular mechanism governing the yield heterosis and high quality of a hybrid rice variety.

scholarly journals Evaluation of Tuber Yield and Marketable Quality of Newly Developed Thirty-Two Potato Varieties Grown in Three Different Ecological Zones in South Korea

Agriculture ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 327 ◽  
Author(s):  
Jahirul Islam ◽  
Sun Phil Choi ◽  
Obyedul Kalam Azad ◽  
Ji Woong Kim ◽  
Young-Seok Lim
Keyword(s):  
South Korea ◽  
Tuber Yield ◽  
High Yield ◽  
Ecological Zones ◽  
Physiological Disorders ◽  
Yield And Quality ◽  
Chip Processing ◽  
Lower Infection ◽  
Low Altitude

The yield and quality of potato in South Korea vary with different environmental conditions and all induced varieties do not perform well in every location. There are many suggested reasons for this problem; they include soil topography, temperature, rainfall pattern, etc. This study focused on finding certain potato genotypes based on high yield and quality (marketable rate, uniformity, less physiological disorders, resistance to diseases), and suitability for processing in three agro-ecological regions in South Korea. Thirty-two potato genotypes were cultivated in three regions i.e., Chuncheon (low altitude), Yang-gu (middle altitude), and Pyeong-chang (alpine), along with three major cultivars (Atlantic, Shepody, and Superior) as control. All the potato lines were evaluated for three consecutive years. The results showed that a higher tuber yield was obtained from the genotypes Gangwon Valley, Valley 11, Valley 13, and Valley 92 in Chuncheon; while Valley 13, Valley 43, and Valley 92 in Yang-gu; and Valley 43, Gui Valley and Valley 92 in Pyeong-chang region with a high marketable quality, and lower infection rates and physiological disorders. The results also showed that higher chip lightness was manifested by the genotypes Juice Valley, Gangwon Valley, Rose Valley, Valley 43, and Valley 91 among the high yielding genotypes. Besides, Pyeong-chang (alpine), a high-altitude region with longer maturation time was found more suitable for potato cultivation, especially for higher tuber yield and higher quality products for potato chip processing.

Persistence of herbage treatment effects in barley and consequences for subsequent crop experimentation

1993 ◽  
Vol 120 (1) ◽  
pp. 25-32
Author(s):  
N. A. Fairey ◽  
L. P. Lefkovitch
Keyword(s):  
Hordeum Vulgare ◽  
Good Yield ◽  
Crop Management ◽  
Barley Grain ◽  
Residual Effects ◽  
High Yield ◽  
Hordeum Vulgare L ◽  
Yield And Quality ◽  
Subsequent Crop

SUMMARYA study, conducted in the Peace region of Canada, determined the effects of the stand and management treatments of four herbage experiments (1986–88) on the yield and quality of subsequent crops of barley (Hordeum vulgare L.) harvested in 1989 and 1990. Quantitative relationships indicated that the crop management treatments most conducive to good yield and quality of herbage were also the most beneficial for subsequent high yield and quality of the barley grain. Unless designs orthogonal to those previously used can be found, covariance adjustment procedures are recommended to minimize the residual effects of previous experiments.

scholarly journals The Role of Cultivation Systems in Quality Sugar Beet Production

2005 ◽  
pp. 134-138
Author(s):  
Kinga Diána Ungai ◽  
Zoltán Győri
Keyword(s):  
Sugar Beet ◽  
Production System ◽  
Plant Protection ◽  
Plant Production ◽  
High Yield ◽  
Yield And Quality ◽  
The Stability ◽  
Saccharose Content

Producing sugar beet, as it is a demanding field crop, has contributed to the raising standard of plant production. It has an outstanding place among the plants that are cultivated in the intensive plant production system. Rentability of sugar manufacture is determined by the stability of yield and the quality (saccharose content) of sugar beet. In this way, the fundamental interest both of the producers and the processing industry is high yield and quality, year by year. The yield and the quality of the sugar beet are mainly determined by the plant production system, so we studied the effect of fertilization, irrigation and plant protection.

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