Understanding the Mechanisms of Positive Microbial Interactions That Benefit Lactic Acid Bacteria Co-cultures

Boza is a traditional low-alcohol fermented beverage from the Balkan Peninsula, frequently explored as a functional food product. The product is rich in Lactic Acid Bacteria (LAB) and some of them can produce bacteriocins. In this study, a sample of Boza from Belogratchik, Bulgaria, was analyzed for the presence of bacteriocinogenic LAB, and after analyses by RAPD-PCR, three representative isolates were characterized by genomic analyses, using whole genome sequencing. Isolates identified as Pediococcus pentosaceus ST75BZ and Pediococcus pentosaceus ST87BZ contained operons encoding for bacteriocins pediocin PA-1 and penocin A, while isolate identified as P. acidilactici ST31BZ contained only the operon for pediocin PA-1 and a CRISPR/Cas system for protection against bacteriophage infection. The antimicrobial activity of bacteriocins produced by the three isolates was inhibited by treatment of the cell-free supernatants with proteolytic enzymes. The produced bacteriocins inhibited the growth of Listeria monocytogenes, Enterococcus spp. and some Lactobacillus spp., among other tested species. The levels of bacteriocin production varied from 3200 AU/ml to 12800 AU/ml recorded against L. monocytogenes 104, 637 and 711, measured at 24 h of incubation at 37oC. All bacteriocins remained active after incubation at pH 2.0 to 10.0. The activity mode of the studied bacteriocins was bactericidal, as determined against L. monocytogenes 104, 637 and 711. In addition, bactericidal activity was demonstrated using a cell leakage β-galactosidase assay, indicating a pore formation mechanism as a mode of action. The present study highlights the importance of combining metagenomic analyses and traditional microbiological approaches as way of characterizing microbial interactions in fermented foods.

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The microbial challenge of winemaking: yeast-bacteria compatibility

FEMS Yeast Research ◽

10.1093/femsyr/foz040 ◽

2019 ◽

Vol 19 (4) ◽

Cited By ~ 7

Author(s):

Louise Bartle ◽

Krista Sumby ◽

Joanna Sundstrom ◽

Vladimir Jiranek

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Microbial Interactions ◽

Physical Parameters ◽

Predicting Success ◽

Physical Interactions ◽

The Impact ◽

Microbial Challenge

ABSTRACTThe diversity and complexity of wine environments present challenges for predicting success of fermentation. In particular, compatibility between yeast and lactic acid bacteria is affected by chemical and physical parameters that are strain and cultivar specific. This review focuses on the impact of compound production by microbes and physical interactions between microbes that ultimately influence how yeast and bacteria may work together during fermentation. This review also highlights the importance of understanding microbial interactions for yeast-bacteria compatibility in the wine context.

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Visualization of Gene Reciprocity among Lactic Acid Bacteria in Yogurt by RNase H-Assisted Rolling Circle Amplification-Fluorescence In Situ Hybridization

Microorganisms ◽

10.3390/microorganisms9061208 ◽

2021 ◽

Vol 9 (6) ◽

pp. 1208

Author(s):

Kyohei Horio ◽

Hirokazu Takahashi ◽

Toshiro Kobori ◽

Kenshi Watanabe ◽

Tsunehiro Aki ◽

...

Keyword(s):

Gene Expression ◽

Lactic Acid ◽

In Situ Hybridization ◽

Lactic Acid Bacteria ◽

Fluorescence In Situ Hybridization ◽

Rolling Circle Amplification ◽

Rnase H ◽

Microbial Interactions ◽

Rolling Circle

Recently, we developed an in situ mRNA detection method termed RNase H-assisted rolling circle amplification-fluorescence in situ hybridization (RHa-RCA-FISH), which can detect even short mRNA in a bacterial cell. However, because this FISH method is sensitive to the sample condition, it is necessary to find a suitable cell permeabilization and collection protocol. Here, we demonstrate its further applicability for detecting intrinsic mRNA expression using lactic acid bacteria (LAB) as a model consortium. Our results show that this method can visualize functional gene expression in LAB cells and can be used for monitoring the temporal transition of gene expression. In addition, we also confirmed that data obtained from bulk analyses such as RNA-seq or microarray do not always correspond to gene expression in individual cells. RHa-RCA-FISH will be a powerful tool to compensate for insufficient data from metatranscriptome analyses while clarifying the carriers of function in microbial consortia. By extending this technique to capture spatiotemporal microbial gene expression at the single-cell level, it will be able to characterize microbial interactions in phytoplankton–bacteria interactions.

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Growth dynamics of lactic acid bacteria and dairy microscopic fungus Geotrichum candidum during their co-cultivation in milk

Food Science and Technology International ◽

10.1177/1082013220976485 ◽

2020 ◽

pp. 108201322097648

Author(s):

Petra Šipošová ◽

Martina Koňuchová ◽

Ľubomír Valík ◽

Alžbeta Medveďová

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Growth Dynamics ◽

Geotrichum Candidum ◽

Maximum Density ◽

Microbial Interactions ◽

Detailed Knowledge ◽

Good Growth ◽

Microscopic Fungus ◽

Average Maximum

Production of high-quality and microbiologically safe fermented dairy products requires controlled growth and microbial interactions between lactic acid bacteria and microscopic fungi. For this purpose, detailed knowledge of their growth characteristics is needed. Therefore, the objective of this study was to analyse the growth dynamics of lactic acid bacteria of commercial DVS® FRESCO® 1000NG culture and dairy isolate of microscopic fungus Geotrichum candidum during their co-cultivation in milk. The growth dynamics of microorganisms was studied in dependence on their initial counts at 12, 15, 18, 21 and 30 °C. Growth parameters were calculated by two primary predictive models, model of Baranyi and Roberts and Huang’s model. Both models showed good ability to describe the growth dynamics of studied microorganisms, as it was confirmed by low values of RMSE index. Both microbial cultures, Fresco culture and Geotrichum candidum, showed good growth ability in milk since they reached the average maximum density of 9.50 ± 0.13 log CFU/mL and 5.85 ± 0.69 log CFU/mL ( n = 45), in order. Maximum density of studied microorganisms was not affected by their initial counts or incubation temperature. On the other hand, effect of mutual ratio of microbial initial counts and increasing temperature had a significant impact on growth dynamics.

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Microbiome: Structures from Lactic Acid Bacteria

PSI Structural Genomics Knowledgebase ◽

10.1038/sbkb.2012.159 ◽

2013 ◽

Author(s):

Arianne Heinrichs

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria

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Lactic acid bacteria and yeasts in kefir grains and kefir made from them

Journal of Industrial Microbiology & Biotechnology ◽

10.1038/sj.jim.7000186 ◽

2002 ◽

Vol 28 (1) ◽

pp. 1-6 ◽

Cited By ~ 7

Author(s):

E Simova ◽

D Beshkova ◽

A Angelov ◽

Ts Hristozova ◽

G Frengova ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Kefir Grains

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Method of separation of major off-odor component and change of components of Gastrodia elata Blume fermented by lactic acid bacteria

10.1055/s-0037-1608346 ◽

2017 ◽

Author(s):

S Young Eun ◽

L In Sok ◽

S Eun Ju ◽

C Min Kyung ◽

H Hyu Ah ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Gastrodia Elata ◽

Gastrodia Elata Blume

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Development of biotechnology for gluten-free sourdough bakery products with a new microbial composition

Khleboproducty ◽

10.32462/0235-2508-2020-29-12-59-63 ◽

2020 ◽

Vol 29 (12) ◽

pp. 59-63

Author(s):

O.I. Parakhina ◽

◽

M.N. Lokachuk ◽

L.I. Kuznetsova ◽

E.N. Pavlovskaya ◽

...

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Starter Culture ◽

Comparative Assessment ◽

Starter Cultures ◽

Bakery Products ◽

Gluten Free ◽

Microbial Composition ◽

Fermentative Activity ◽

Theoretical Foundations

The research was carried out within the framework of the theme of state assignment № 0593–2019–0008 «To develop theoretical foundations for creating composite mixtures for bakery products using physical methods of exposure that ensure homogeneity, stability of mixtures and bioavailability of nutrients, to optimize diets population of Russia». The data on the species belonging of new strains of lactic acid bacteria and yeast isolated from samples of good quality gluten-free starter cultures are presented. A comparative assessment of the antagonistic and acid-forming activity of strains of lactic acid bacteria and the fermentative activity of yeast was carried out. The composition of microbial compositions from selected strains of LAB and yeast was developed. The influence of the starter culture on the new microbial composition on the physicochemical, organoleptic indicators of the bread quality and resistance to mold and ropy-disease was investigated.

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