scholarly journals m5C RNA Methylation Primarily Affects the ErbB and PI3K–Akt Signaling Pathways in Gastrointestinal Cancer

2020 ◽  
Vol 7 ◽  
Author(s):  
Shixin Xiang ◽  
Yongshun Ma ◽  
Jing Shen ◽  
Yueshui Zhao ◽  
Xu Wu ◽  
...  

5-Methylcytosine (m5C) is a kind of methylation modification that occurs in both DNA and RNA and is present in the highly abundant tRNA and rRNA. It has an important impact on various human diseases including cancer. The function of m5C is modulated by regulatory proteins, including methyltransferases (writers) and special binding proteins (readers). This study aims at comprehensive study of the m5C RNA methylation-related genes and the main pathways under m5C RNA methylation in gastrointestinal (GI) cancer. Our result showed that the expression of m5C writers and reader was mostly up-regulated in GI cancer. The NSUN2 gene has the highest proportion of mutations found in GI cancer. Importantly, in liver cancer, higher expression of almost all m5C regulators was significantly associated with lower patient survival rate. In addition, the expression level of m5C-related genes is significantly different at various pathological stages. Finally, we have found through bioinformatics analysis that m5C regulatory proteins are closely related to the ErbB/PI3K–Akt signaling pathway and GSK3B was an important target for m5C regulators. Besides, the compound termed streptozotocin may be a key candidate drug targeting on GSK3B for molecular targeted therapy in GI cancer.

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Tulika Das ◽  
Surasree Pal ◽  
Agneyo Ganguly

Abstract RecQ helicases are a highly conserved class of DNA helicases that play crucial role in almost all DNA metabolic processes including replication, repair and recombination. They are able to unwind a wide variety of complex intermediate DNA structures that may result from cellular DNA transactions and hence assist in maintaining genome integrity. Interestingly, a huge number of recent reports suggest that many of the RecQ family helicases are directly or indirectly involved in regulating transcription and gene expression. On one hand, they can remove complex structures like R-loops, G-quadruplexes or RNA:DNA hybrids formed at the intersection of transcription and replication. On the other hand, emerging evidence suggests that they can also regulate transcription by directly interacting with RNA polymerase or recruiting other protein factors that may regulate transcription. This review summarizes the up to date knowledge on the involvement of three human RecQ family proteins BLM, WRN and RECQL5 in transcription regulation and management of transcription associated stress.


2014 ◽  
Vol 83 (1) ◽  
pp. 585-614 ◽  
Author(s):  
Li Shen ◽  
Chun-Xiao Song ◽  
Chuan He ◽  
Yi Zhang

2020 ◽  
Author(s):  
Xing Meng ◽  
Bruce Tsurutani ◽  
Anthony Mannucci

<p>We present a comprehensive study of all 39 superstorms (minimum Dst ≤ −250 nT) occurring from 1957 to present including analyzing their main phase developments, seasonal and solar cycle dependences, as well as their solar and interplanetary causes. We find that 87% of the superstorms have a multistep main phase development or are built upon preceding geomagnetic activities, and 90% of the superstorms occurred either near solar maximum or during the declining phase.  For the superstorm association with solar activities, 54% of the superstorms were associated with X‐class solar flares, 36% were associated with M‐class flares, and 5% with C‐class flares. All solar flares related to superstorms occurred in active regions, indicating the importance of active regions to superstorms. Most flares were located in the central meridian or slightly west of it as expected. For the interplanetary conditions leading to the development of the superstorm main phase, 95% of the 19 superstorms with available solar wind data are solely caused or partially caused by the sheath anti-sunward of an interplanetary coronal mass ejection (ICME), indicating the importance of the sheath structure in driving superstorms. For eight superstorms that have identifiable interplanetary shocks preceding the ICMEs, the shock normal angles were almost all quasi‐perpendicular. Larger shock normal angles statistically corresponded to greater superstorm intensities.</p>


Author(s):  
Zhanna Matviishyna

Nowadays, cultural monuments deserve increasing attention. A comprehensive study of archeological complexes enables us to more accurately reproduce the conditions of formation and evolution of ancient society. In recent decades, archaeological sites have been investigated using archeology and paleogeography methods to reconstruct the character of landscape changes in the study area. Among such historical and archeological complexes is the famous city of Baturin, which holds a special place for deep and comprehensive study. Less than half a century later, having traveled from a small outpost on the northeastern border of the Commonwealth to the hetman's capital, Baturin died in the flames of the Northern War in the same short period of time. Tragedy of 1708 made Baturin not only a national shrine, but also a reference archaeological monument of the Cossack era, a cultural layer whose level of saturation and informativeness is unmatched. The inter-river basin of the Desna and the Seimas, which is geographically included in the Baturin Family, is one of the most important watersheds of the Dnieper North Left Bank, and the Seim River itself is a kind of natural border between the forest-steppe and forest zones. The territory where Baturin originated had a number of significant advantages. Baturin is located on the edge of the high terrace of the Diet. It is the only such elevated area of the left bank terrace of the Diet in its lower stream, which allowed to control virtually all of its basin. ancient times, which, combined with the wide floodplain of the Seimas with its opportunities for the development of livestock, fishing, hunting, created optimal conditions for economic activity of the population. During paleopedological research it was established that in the territory of the study in the catenary of soils with artifacts of the Bronze Age developed soils of meadow and forest-meadow genesis with well-formed humus horizons close to meadow-sod and alluvial-sod zones of temperate, but temperate climate. In the clearing of the shaft, the findings of the early Iron Age are confined to the surface of the humus horizon of the sod of slightly podzolic soils, formed in the conditions of meadow-steppe landscapes of temperate climate, less humid than the modern (background) soil. In the XVII-XVIII centuries. In the Polissya conditions, meadow-forest landscapes became widespread. In the soils on the high grass meadows there were conditions for the accumulation of organic matter, which made it possible to form well-defined humus horizons and the processes of sod genesis were quite pronounced. Turf-slightly podzolic ash-sandy sandy loamy and sandy soils of the XVII-XVIII centuries had well-defined humus horizons, and often were deeply humus, which provided for their agricultural use with ease of machining. The proximity of a fairly powerful river, as well as the spread of forests (as timber for business purposes), increased the possibility of comfortable living conditions in this area. In the XVIII-XX centuries. Opportunities for cultivation of soils with fertilizers have emerged and soils of clearing № 5,7,8 are characterized by high and deep (almost all over the profile) humus. They are an example of human influence on the cultivation of land and landscapes. In the floodplains, alluvial-marsh silt soils, enriched with the content of Fe and Mn hydroxides, often formed the basis for the development of iron-smelting production in the territories close to Baturin. In the low and high floodplains of the Diet, the formation of sediments was associated with alluvial processes (floods, alluvial sands) and aerial ones (sands, dunes). Among modern Baturin soils (background), typical sod-medium- and highly podzolic, as opposed to the predominance of slightly podzolic sod, aszed alluvial-meadow and alluvial-sod soils are identified. The terraces are characterized by the development of sod, sod-alluvial and sod-swamp soils (the latter on floodplains).


Author(s):  
J. Seshu ◽  
Trever C. Smith ◽  
Ying-Han Lin ◽  
S. L. Rajasekhar Karna ◽  
Christine L. Miller ◽  
...  

Minerals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 127
Author(s):  
Karel Breiter ◽  
Hans-Jürgen Förster

A comprehensive study of monazite–cheralite–huttonite solid solutions (s.s.) and xenotime from the highly evolved, strongly peraluminous P–F–Li-rich Podlesí granite stock in the Krušné Hory Mts., Czech Republic, indicates that, with the increasing degree of magmatic and high-T early post-magmatic evolution, the content of the cheralite component in monazite increases and the relative dominance of middle rare earth elements (MREE) in xenotime becomes larger. Considering the overall compositional signatures of these two accessory minerals in the late Variscan granites of the Erzgebirge/Krušné Hory Mts., three types of granites can be distinguished: (i) chemically less evolved F-poor S(I)- and A-type granites contain monazite with a smooth, mostly symmetric chondrite-normalized (CN) rare-earth elements (REE) pattern gradually declining from La to Gd; associated xenotime is Y-rich (˃0.8 apfu Y) with a flat MREE–HREE (heavy rare earth elements) pattern; (ii) fractionated A-type granites typically contain La-depleted monazite with Th accommodated as the huttonite component, combined with usually Y-poor (0.4–0.6 apfu Y) xenotime characterized by a smoothly inclining, Yb–Lu-dominant CN-REE pattern; (iii) fractionated peraluminous Li-mica granites host monazite with a flat, asymmetric (kinked at La and Nd) CN-LREE pattern, with associated xenotime distinctly MREE (Gd–Tb–Dy)-dominant. Monazite and xenotime account for the bulk of the REE budgets in all types of granite. In peraluminous S(I)-type granites, which do not bear thorite, almost all Th is accommodated in monazite–cheralite s.s. In contrast, Th budgets in A-type granites are accounted for by monazite–huttonite s.s. together with thorite. The largest portion of U is accommodated in uraninite, if present.


2021 ◽  
Vol 22 (22) ◽  
pp. 12193
Author(s):  
Lassi Koski ◽  
Cecilia Ronnevi ◽  
Elina Berntsson ◽  
Sebastian K. T. S. Wärmländer ◽  
Per M. Roos

Amyotrophic lateral sclerosis (ALS), Alzheimer’s disease, Parkinson’s disease and similar neurodegenerative disorders take their toll on patients, caregivers and society. A common denominator for these disorders is the accumulation of aggregated proteins in nerve cells, yet the triggers for these aggregation processes are currently unknown. In ALS, protein aggregation has been described for the SOD1, C9orf72, FUS and TDP-43 proteins. The latter is a nuclear protein normally binding to both DNA and RNA, contributing to gene expression and mRNA life cycle regulation. TDP-43 seems to have a specific role in ALS pathogenesis, and ubiquitinated and hyperphosphorylated cytoplasmic inclusions of aggregated TDP-43 are present in nerve cells in almost all sporadic ALS cases. ALS pathology appears to include metal imbalances, and environmental metal exposure is a known risk factor in ALS. However, studies on metal-to-TDP-43 interactions are scarce, even though this protein seems to have the capacity to bind to metals. This review discusses the possible role of metals in TDP-43 aggregation, with respect to ALS pathology.


2021 ◽  
Vol 4 (14) ◽  
pp. 13-22
Author(s):  
Zul Aizam Yassin ◽  
Mohammad Rusdi Ab Majid ◽  
Aznida Aziz ◽  
Nurhijrah Zakaria

Internet addiction among teenagers is an important issue discussed around the world. Almost all experts cite this issue as part of the main problems the country faces. While teenagers are the main group involved in this issue. This study uses quantitative methods as the main design. This survey study involved respondents consisting of 614 trainee teachers selected according to IPG zones throughout Malaysia. A descriptive statistical approach was used to obtain the level of internet addiction. While the inferential statistical method is used to see the relationship between the variables that have been set. The questionnaire instrument was adapted from An Internet Addiction Scale by Kimberly (1998) which was modified to coincide with the study conducted. The analysis was conducted using SPSS and analyzed descriptively to explain the findings of the study. The findings of this study as a whole found that internet addiction among students at IPG is at a moderate level where it is still under control and can be managed well without interfering with their daily lives. It is hoped that this study can provide good input to all parties to formulate actions for the well-being of society by forming a new generation that is concerned about the selection and use of information resources on the internet effectively.


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