scholarly journals Best Practices for Technical Reproducibility Assessment of Multiplex Immunofluorescence

2021 ◽  
Vol 8 ◽  
Author(s):  
Caddie Laberiano-Fernández ◽  
Sharia Hernández-Ruiz ◽  
Frank Rojas ◽  
Edwin Roger Parra
Keyword(s):  
Best Practices ◽  
Signal Amplification ◽  
Data Generation ◽  
Technical Requirements ◽  
Analysis Strategies ◽  
Technical Reproducibility ◽  
High Flexibility ◽  
Correct Data ◽  
Cancer Studies ◽  
Single Slide

Multiplex immunofluorescence (mIF) tyramide signal amplification is a new and useful tool for the study of cancer that combines the staining of multiple markers in a single slide. Several technical requirements are important to performing high-quality staining and analysis and to obtaining high internal and external reproducibility of the results. This review manuscript aimed to describe the mIF panel workflow and discuss the challenges and solutions for ensuring that mIF panels have the highest reproducibility possible. Although this platform has shown high flexibility in cancer studies, it presents several challenges in pre-analytic, analytic, and post-analytic evaluation, as well as with external comparisons. Adequate antibody selection, antibody optimization and validation, panel design, staining optimization and validation, analysis strategies, and correct data generation are important for reproducibility and to minimize or identify possible issues during the mIF staining process that sometimes are not completely under our control, such as the tissue fixation process, storage, and cutting procedures.

scholarly journals Procedural Requirements and Recommendations for Multiplex Immunofluorescence Tyramide Signal Amplification Assays to Support Translational Oncology Studies

Cancers ◽  
2020 ◽  
Vol 12 (2) ◽  
pp. 255 ◽  
Author(s):  
Edwin Roger Parra ◽  
Mei Jiang ◽  
Luisa Solis ◽  
Barbara Mino ◽  
Caddie Laberiano ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Cancer Biology ◽  
Tyramide Signal Amplification ◽  
Technical Requirements ◽  
Oncology Research ◽  
Translational Oncology ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Single Slide ◽  
Formalin Fixed

In the development of a multiplex immunofluorescence (IF) platform and the optimization and validation of new multiplex IF panels using a tyramide signal amplification system, several technical requirements are important for high-quality staining, analysis, and results. The aim of this review is to discuss the basic requirements for performing multiplex IF tyramide signal amplification (TSA) in formalin-fixed, paraffin-embedded cancer tissues to support translational oncology research. Our laboratory has stained approximately 4000 formalin-fixed, paraffin-embedded tumor samples using the multiplex IF TSA system for immune profiling of several labeled biomarkers in a single slide to elucidate cancer biology at a protein level and identify therapeutic targets and biomarkers. By analyzing several proteins in thousands of cells on a single slide, this technique provides a systems-level view of various processes in various tumor tissues. Although this technology shows high flexibility in cancer studies, it presents several challenges when applied to study different histology cancers. Our experience shows that adequate antibody validation, staining optimization, analysis strategies, and data generation are important steps for generating quality results. Tissue management, fixation procedures, storage, and cutting can also affect the results of the assay and must be standardized. Overall, this method is reliable for supporting translational research given a precise, step-by-step approach.

scholarly journals Isabl Platform, a digital biobank for processing multimodal patient data

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Juan S. Medina-Martínez ◽  
Juan E. Arango-Ossa ◽  
Max F. Levine ◽  
Yangyu Zhou ◽  
Gunes Gundem ◽  
...  
Keyword(s):  
Best Practices ◽  
High Throughput ◽  
Web Application ◽  
Continuous Data ◽  
Data Provenance ◽  
Command Line ◽  
Data Generation ◽  
Data Utilization ◽  
Meta Analyses ◽  
Patient Centric

Abstract Background The widespread adoption of high throughput technologies has democratized data generation. However, data processing in accordance with best practices remains challenging and the data capital often becomes siloed. This presents an opportunity to consolidate data assets into digital biobanks—ecosystems of readily accessible, structured, and annotated datasets that can be dynamically queried and analysed. Results We present Isabl, a customizable plug-and-play platform for the processing of multimodal patient-centric data. Isabl's architecture consists of a relational database (Isabl DB), a command line client (Isabl CLI), a RESTful API (Isabl API) and a frontend web application (Isabl Web). Isabl supports automated deployment of user-validated pipelines across the entire data capital. A full audit trail is maintained to secure data provenance, governance and ensuring reproducibility of findings. Conclusions As a digital biobank, Isabl supports continuous data utilization and automated meta analyses at scale, and serves as a catalyst for research innovation, new discoveries, and clinical translation.

scholarly journals Implementation of a Unified Billing System in Housing and Communal Services

2020 ◽  
Vol 6 (6) ◽  
pp. 205-210
Author(s):  
O. Mamanazarov ◽  
D. Inoyatova
Keyword(s):  
Best Practices ◽  
Public Services ◽  
International Experience ◽  
Quality Of Services ◽  
Billing System ◽  
Technical Requirements ◽  
Program Support ◽  
Regional Authorities ◽  
Modern Russian

In this article, the main topics and goals of the work such as the study of billing systems of public services and the development of requirements, determining the needs in the system being created, analyzing the world’s best practices for combining all public services in one information field, determining ways to create a complex of unified billing system taking into account common technical requirements for billing systems, determining ways to develop and update the software to improve the quality of services to consumers in a single billing system are widely revealed. It is also worth noting that in this article, based on the study of modern Russian and international experience in implementing and improving a unified billing system in the industry of housing and communal services analyzed the existing barriers and the development of the industry, describes the main opportunities and promising areas of program support, organizational-economic and administrative measures to improve accessibility and improve housing and utilities and minimize utility costs of the population. Specific directions for Federal and international regional authorities to increase the investment attractiveness of innovative housing and communal services and the transition of this industry to an innovative vector of development are proposed.

scholarly journals Big Data in Biodiversity Science: A Framework for Engagement

Technologies ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 60
Author(s):  
Tendai Musvuugwa ◽  
Muxe Gladmond Dlomu ◽  
Adekunle Adebowale
Keyword(s):  
Big Data ◽  
Best Practices ◽  
Scientific Community ◽  
Data Driven ◽  
Data Generation ◽  
Global Trends ◽  
Major Threat ◽  
Biodiversity Science ◽  
Evidenced Based ◽  
Historical Developments

Despite best efforts, the loss of biodiversity has continued at a pace that constitutes a major threat to the efficient functioning of ecosystems. Curbing the loss of biodiversity and assessing its local and global trends requires a vast amount of datasets from a variety of sources. Although the means for generating, aggregating and analyzing big datasets to inform policies are now within the reach of the scientific community, the data-driven nature of a complex multidisciplinary field such as biodiversity science necessitates an overarching framework for engagement. In this review, we propose such a schematic based on the life cycle of data to interrogate the science. The framework considers data generation and collection, storage and curation, access and analysis and, finally, communication as distinct yet interdependent themes for engaging biodiversity science for the purpose of making evidenced-based decisions. We summarize historical developments in each theme, including the challenges and prospects, and offer some recommendations based on best practices.

A Survey of Speech-Language Pathologists' Approaches to Assessing Social Communication Disorders in Children

2020 ◽  
pp. 1-17
Author(s):  
Kristen Izaryk ◽  
Robin Edge ◽  
Dawn Lechwar
Keyword(s):  
Best Practices ◽  
Communication Disorders ◽  
Assessment Tools ◽  
Self Report ◽  
Speech Language Pathologists ◽  
Teacher Report ◽  
Formal Assessment ◽  
Language Sampling ◽  
Specific Assessment ◽  
Social Communication Disorders

Purpose The purpose of this article is to explore and describe the approaches and specific assessment tools that speech-language pathologists are currently using to assess social communication disorders (SCDs) in children, in relation to current best practices. Method Ninety-four speech-language pathologists completed an online survey asking them to identify which of the following approaches they use to assess children with SCD: parent/teacher report, naturalistic observation, formal assessment, language sample analysis, interviews, semistructured tasks, and peer/self-report. Participants were also asked to identify specific assessment tools they use within each approach. Results Participants most commonly assess SCDs by combining interviews, naturalistic observation, language sampling, parent/teacher report, and formal assessment. Semistructured tasks and peer/self-report tools were less frequently utilized. Several established parent/teacher report and formal assessment tools were commonly identified for assessing SCDs. Most participants use an informal approach for interviews, language sampling, and naturalistic observations in their SCD assessment process. Conclusions Generally, participants follow best practices for assessing SCDs by combining several different approaches. Some considerations for future assessment are identified, including the use of established protocols in the place of informal approaches in order to make the assessment of SCDs more systematic. Future directions for research are discussed.

Practical Resources for Provision of Services to Culturally and Linguistically Diverse Users of AAC

2015 ◽  
Vol 22 (1) ◽  
pp. 25-39
Author(s):  
Elena Dukhovny ◽  
E. Betsy Kelly
Keyword(s):  
Best Practices ◽  
Augmentative And Alternative Communication ◽  
Linguistically Diverse ◽  
Intervention Strategies ◽  
Culturally And Linguistically Diverse ◽  
Limited Resources ◽  
Flexible Systems ◽  
Alternative Communication ◽  
Practical Applications ◽  
Provision Of Services

According to the 2010 U.S. Census, over 20% of Americans speak a language other than English in the home, with Spanish, Chinese, and French being the languages most commonly spoken, aside from English. However, few augmentative and alternative communication (AAC) systems offer multilingual support for individuals with limited functional speech. There has been much discussion in the AAC community about best practices in AAC system design and intervention strategies, but limited resources exist to help us provide robust, flexible systems for users who speak languages other than English. We must provide services that take into consideration the unique needs of culturally and linguistically diverse users of AAC and help them reach their full communication potential. This article outlines basic guidelines for best practices in AAC design and selection, and presents practical applications of these best practices to multilingual/multicultural clients.

Autism, AAC, and Proloquo2Go

2009 ◽  
Vol 18 (4) ◽  
pp. 137-145 ◽  
Author(s):  
Samuel Sennott ◽  
Adam Bowker
Keyword(s):  
Best Practices ◽  
Augmentative And Alternative Communication ◽  
Communication Technologies ◽  
Autism Spectrum ◽  
Alternative Communication ◽  
Visual Supports ◽  
Digital Device ◽  
Ipod Touch ◽  
Spectrum Disorders ◽  
Communication Needs

People with ASD often need to access AAC in situations where a tabletop digital device is not practical. Recent advancements have made more powerful, portable, and affordable communication technologies available to these individuals. Proloquo2Go is a new portable augmentative and alternative communication system that runs on an iPhone or iPod touch and can be used to meet the diverse needs of individuals with autism spectrum disorders (ASD) who are ambulatory and have difficulty using speech to meet their full daily communication needs. This article examines Proloquo2Go in light of the best practices in AAC for individuals with ASD such as symbols, visual supports, voice output, and inclusion.

The Developing Relationship Among Cognition, Amplification, and Aural Rehabilitation

2016 ◽  
Vol 1 (6) ◽  
pp. 47-54 ◽  
Author(s):  
Jeffrey J. DiGiovanni ◽  
Travis L. Riffle
Keyword(s):  
Working Memory ◽  
Best Practices ◽  
Cognitive Abilities ◽  
Hearing Aid ◽  
Hearing Aid Fitting ◽  
Aural Rehabilitation ◽  
Basic Concepts ◽  
And Function

The search for best practices in hearing aid fittings and aural rehabilitation has generally used the audiogram and function stemming from peripheral sensitivity. In recent years, however, we have learned that individuals respond differently to various hearing aid and aural rehabilitation techniques based on cognitive abilities. In this paper, we review basic concepts of working memory and the literature driving our knowledge in newer concepts of hearing aid fitting and aural rehabilitation.

Sign in / Sign up

Export Citation Format

Share Document