scholarly journals Biomaterials for Neural Tissue Engineering

2021 ◽  
Vol 3 ◽  
Author(s):  
Laura Rodríguez Doblado ◽  
Cristina Martínez-Ramos ◽  
Manuel Monleón Pradas
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Peripheral Nerve Injury ◽  
Neural Tissue ◽  
Natural Polymers ◽  
Nerve Injuries ◽  
Neural Tissue Engineering ◽  
Axonal Regrowth ◽  
Cord Injury ◽  
Synthetic And Natural Polymers

The therapy of neural nerve injuries that involve the disruption of axonal pathways or axonal tracts has taken a new dimension with the development of tissue engineering techniques. When peripheral nerve injury (PNI), spinal cord injury (SCI), traumatic brain injury (TBI), or neurodegenerative disease occur, the intricate architecture undergoes alterations leading to growth inhibition and loss of guidance through large distance. To improve the limitations of purely cell-based therapies, the neural tissue engineering philosophy has emerged. Efforts are being made to produce an ideal scaffold based on synthetic and natural polymers that match the exact biological and mechanical properties of the tissue. Furthermore, through combining several components (biomaterials, cells, molecules), axonal regrowth is facilitated to obtain a functional recovery of the neural nerve diseases. The main objective of this review is to investigate the recent approaches and applications of neural tissue engineering approaches.

scholarly journals Tissue Engineering Strategies for Peripheral Nerve Regeneration

2021 ◽  
Vol 12 ◽  
Author(s):  
Yin Li ◽  
Zhenjiang Ma ◽  
Ya Ren ◽  
Dezhi Lu ◽  
Tao Li ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Peripheral Nerve ◽  
Nerve Regeneration ◽  
Peripheral Nerve Injury ◽  
Peripheral Nerve Regeneration ◽  
Neural Tissue ◽  
Nerve Tissue ◽  
Neural Tissue Engineering ◽  
Clinical Problems

A peripheral nerve injury (PNI) has severe and profound effects on the life of a patient. The therapeutic approach remains one of the most challenging clinical problems. In recent years, many constructive nerve regeneration schemes are proposed at home and abroad. Nerve tissue engineering plays an important role. It develops an ideal nerve substitute called artificial nerve. Given the complexity of nerve regeneration, this review summarizes the pathophysiology and tissue-engineered repairing strategies of the PNI. Moreover, we discussed the scaffolds and seed cells for neural tissue engineering. Furthermore, we have emphasized the role of 3D printing in tissue engineering.

Spinal Cord Neuronal Cell Properties Respond Differentially to the Stiffness of DNA Crosslinked Hydrogels

2008 ◽  
Author(s):  
Frank X. Jiang ◽  
Penelope Georges ◽  
Uday Chippada ◽  
Lulu Li ◽  
Bernard Yurke ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Spinal Cord ◽  
Central Nervous System ◽  
Neuronal Cell ◽  
Neural Tissue ◽  
Cell Types ◽  
Extracellular Matrices ◽  
Neural Tissue Engineering ◽  
Cord Injury ◽  
Cns Neurons

Mechanical cues arising from extracellular matrices greatly affect cellular properties, and hence, are of significance in designing biomaterials. Similar to many other cell types, including fibroblasts and hepatocytes, central nervous system (CNS) neurons have been found to exhibit distinct responses to the stiffness of the substrates they reside on [1]. There is an increasing awareness that mechanical properties also play a key role in successful utilization of scaffolds for those tissues whose major functions are not load-bearing, such as the spinal cord. In light of this, there is a growing interest in incorporating mechanical cues in biomaterial design for neural tissue engineering applications, including spinal cord injury.

scholarly journals Engineering Biocompatible Hydrogel Fibers With Tunable Mechanical Properties For Neural Tissue Engineering

2021 ◽  
Author(s):  
Roya Samanipour ◽  
Hamed Tahmooressi ◽  
Hojatollah Rezaei Nejad ◽  
Kabilan Sakthivel ◽  
Adel Yavarinasab ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Neuroblastoma Cell ◽  
Neural Tissue ◽  
Cellular Growth ◽  
Great Promise ◽  
Human Neuroblastoma Cell ◽  
Neural Tissue Engineering ◽  
Human Neuroblastoma ◽  
Tunable Mechanical Properties

Abstract Neural tissue engineering holds a great promise for the treatment of neurodegenerative diseases and peripheral nerve injuries. However, the anisotropic mechanical and electrical properties of the highly aligned neural cells have hindered the development of a faithful in vitro disease models. In this study, a core-shell microfluidic extrusion method is implemented to fabricate a cell-laden composite hydrogel fiber with tunable mechanical properties. The hybrid hydrogel was formed using a core of GelMA mixed with gelatin seeded with human neuroblastoma cell (SH-SY5Y) and an alginate shell. The composition of the core hydrogel was optimized to support cellular growth and differentiation, yet allow a feasible fabrication of cell-laden fibers. The engineered fibers were remarkably biocompatible and enabled the formation of highly aligned cellular morphology.

scholarly journals Physical and Mechanical Characterization of Fibrin-Based Bioprinted Constructs Containing Drug-Releasing Microspheres for Neural Tissue Engineering Applications

Processes ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 1205
Author(s):  
Ruchi Sharma ◽  
Rebecca Kirsch ◽  
Karolina Papera Valente ◽  
Milena Restan Perez ◽  
Stephanie Michelle Willerth
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Loss Modulus ◽  
Physical And Mechanical Properties ◽  
Neural Tissue ◽  
Neuronal Cultures ◽  
Neural Cells ◽  
Neural Tissue Engineering ◽  
Mechanical Reinforcement ◽  
Tissue Constructs

Three-dimensional bioprinting can fabricate precisely controlled 3D tissue constructs. This process uses bioinks—specially tailored materials that support the survival of incorporated cells—to produce tissue constructs. The properties of bioinks, such as stiffness and porosity, should mimic those found in desired tissues to support specialized cell types. Previous studies by our group validated soft substrates for neuronal cultures using neural cells derived from human-induced pluripotent stem cells (hiPSCs). It is important to confirm that these bioprinted tissues possess mechanical properties similar to native neural tissues. Here, we assessed the physical and mechanical properties of bioprinted constructs generated from our novel microsphere containing bioink. We measured the elastic moduli of bioprinted constructs with and without microspheres using a modified Hertz model. The storage and loss modulus, viscosity, and shear rates were also measured. Physical properties such as microstructure, porosity, swelling, and biodegradability were also analyzed. Our results showed that the elastic modulus of constructs with microspheres was 1032 ± 59.7 Pascal (Pa), and without microspheres was 728 ± 47.6 Pa. Mechanical strength and printability were significantly enhanced with the addition of microspheres. Thus, incorporating microspheres provides mechanical reinforcement, which indicates their suitability for future applications in neural tissue engineering.

scholarly journals Spidroin Silk Fibers with Bioactive Motifs of Extracellular Proteins for Neural Tissue Engineering

ACS Omega ◽  
2021 ◽  
Author(s):  
Veronica A. Revkova ◽  
Konstantin V. Sidoruk ◽  
Vladimir A. Kalsin ◽  
Pavel A. Melnikov ◽  
Mikhail A. Konoplyannikov ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Neural Tissue ◽  
Extracellular Proteins ◽  
Neural Tissue Engineering ◽  
Silk Fibers

scholarly journals Electrospun Nanofibrous Materials for Neural Tissue Engineering

Polymers ◽  
2011 ◽  
Vol 3 (1) ◽  
pp. 413-426 ◽  
Author(s):  
Yee-Shuan Lee ◽  
Treena Livingston Arinzeh
Keyword(s):  
Tissue Engineering ◽  
Neural Tissue ◽  
Neural Tissue Engineering

Directing Axonal Growth: A Review on the Fabrication of Fibrous Scaffolds That Promotes the Orientation of Axons

Gels ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 25
Author(s):  
Devindraan Sirkkunan ◽  
Belinda Pingguan-Murphy ◽  
Farina Muhamad
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Neuronal Cells ◽  
Neural Tissue ◽  
Axonal Growth ◽  
Structural Proteins ◽  
Neural Cells ◽  
Neural Tissue Engineering ◽  
Fibrous Scaffolds ◽  
Specialized Function

Tissues are commonly defined as groups of cells that have similar structure and uniformly perform a specialized function. A lesser-known fact is that the placement of these cells within these tissues plays an important role in executing its functions, especially for neuronal cells. Hence, the design of a functional neural scaffold has to mirror these cell organizations, which are brought about by the configuration of natural extracellular matrix (ECM) structural proteins. In this review, we will briefly discuss the various characteristics considered when making neural scaffolds. We will then focus on the cellular orientation and axonal alignment of neural cells within their ECM and elaborate on the mechanisms involved in this process. A better understanding of these mechanisms could shed more light onto the rationale of fabricating the scaffolds for this specific functionality. Finally, we will discuss the scaffolds used in neural tissue engineering (NTE) and the methods used to fabricate these well-defined constructs.

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