Inhibition of Advanced Glycation End Products in Yogurt by Lotus Seedpod Oligomeric Procyanidin

The basic ingredients of yogurt include lactose and protein. Yogurt undergoes the Maillard reaction easily, producing many advanced glycation end products (AGEs) that cause some chronic diseases. Lotus seedpod oligomeric procyanidin (LSOPC) have demonstrated a strong inhibitory effect on AGE formation in simulated models; however, the inhibition of procyanidin on AGE formation and the subsequent effects on yogurt quality remains unknown. Our study demonstrated that LSOPC had a good inhibitory effect on the formation of fluorescent AGEs and Nε-carboxymethyl lysine (P < 0.05). The inhibitory capacity on AGEs and antioxidant activity of yogurt were positively correlated with the concentration of LSOPC. The effect of LSOPC on the physicochemical properties of yogurt was also evaluated. Bound water content, viscosity, and flavor of yogurt were significantly increased after LSOPC addition (P < 0.05). Therefore, LSOPC may lead to significant benefits for controlling AGE formation and improving the quality of yogurt.

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Advanced glycation end-products associate with podocytopathy in type II diabetic patients

10.21203/rs.3.rs-192615/v1 ◽

2021 ◽

Author(s):

Rajkishor Nishad ◽

Tahaseen V Syed ◽

Manga Motrapu ◽

Rajesh Kavvuri ◽

Kiranmayi Kodali ◽

...

Keyword(s):

Kidney Disease ◽

Kidney Function ◽

Advanced Glycation End Products ◽

Diabetic Patients ◽

Type Ii ◽

Advanced Glycation ◽

Mesenchymal Transition ◽

Glycation End Products ◽

End Products ◽

Carboxymethyl Lysine

Abstract Background The prevalence of diabetes reaches epidemic proportions, affecting the incidence of diabetic nephropathy (DN) and associated end-stage kidney disease (ESKD). Diabetes is the leading cause of ESKD since 30–40% of diabetic patients develop DN. Albuminuria and eGFR have been considered a surrogate outcome of chronic kidney disease, and the search for a biomarker that predicts progression to diabetic kidney disease is intense.Methods We analyzed the association of serum advanced glycation end-products (AGEs) index (AGI) with impaired kidney function in uncontrolled diabetic patients (type II, n = 130) with albuminuria ranging from (150 to 450 mg/day). The kidney biopsy specimens were also examined for the association of AGEs, particularly carboxymethyl lysine (CML) with kidney function. Further, we also assessed the effect of carboxymethyl lysine on glomerular injury and podocytopathy in experimental animals.Results We observed a strong correlation between AGI and impaired kidney function in miroalbuminuric patients with hyperglycemia. A significant association between CML levels and impaired kidney function was noticed. Administration of CML in mice showed heavy proteinuria and glomerular abnormalities. Reduced podocyte number observed in mice administered with CML could be attributed to the epithelial-mesenchymal transition (EMT) of podocytes. Conclusion Serum AGEs could be independently related to the podocyte injury vis-a-vis the risk of DN progression to ESKD in patients with microalbuminuria. AGEs or CML could be considered a prognostic marker to assess microalbuminuria progression to ESKD in diabetic patients.

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In vitro inhibitory effect of selected parts of Moringa oleifera against the formation of major types of advanced glycation end-products

Acta Horticulturae ◽

10.17660/actahortic.2021.1306.16 ◽

2021 ◽

pp. 135-142

Author(s):

O.I. Adeniran ◽

M.A. Mogale

Keyword(s):

Advanced Glycation End Products ◽

Moringa Oleifera ◽

Inhibitory Effect ◽

Advanced Glycation ◽

Glycation End Products ◽

End Products

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Inhibitory Activities ofStauntonia hexaphyllaLeaf Constituents on Rat Lens Aldose Reductase and Formation of Advanced Glycation End Products and Antioxidant

BioMed Research International ◽

10.1155/2017/4273257 ◽

2017 ◽

Vol 2017 ◽

pp. 1-8 ◽

Cited By ~ 13

Author(s):

Seung Hwan Hwang ◽

Shin Hwa Kwon ◽

Set Byeol Kim ◽

Soon Sung Lim

Keyword(s):

Aldose Reductase ◽

Advanced Glycation End Products ◽

Inhibitory Activity ◽

Solvent System ◽

Inhibitory Effect ◽

Advanced Glycation ◽

Rat Lens Aldose Reductase ◽

Glycation End Products ◽

End Products ◽

Etoac Fraction

Stauntonia hexaphylla(Thunb.) Decne. (Lardizabalaceae) leaves (SHL) have been used traditionally as analgesics, sedatives, diuretics, and so on, in China. To date, no data have been reported on the inhibitory effect of SHL and its constituents on rat lens aldose reductase (RLAR) and advanced glycation end products (AGEs). Therefore, the inhibitory effect of compounds isolated from SHL extract on RLAR and AGEs was investigated to evaluate potential treatments of diabetic complications. The ethyl acetate (EtOAC) fraction of SHL extract showed strong inhibitory activity on RLAR and AGEs; therefore, EtOAc fraction (3.0 g) was subjected to Sephadex LH-20 column chromatography, for further fractionation, with 100% MeOH solvent system to investigate its effect on RLAR and AGEs. Phytochemical investigation of SHL led to the isolation of seven compounds. Among the isolated compounds, chlorogenic acid, calceolarioside B, luteolin-3′-O-β-D-glucopyranoside, quercetin-3-O-β-D-glucopyranoside, and luteolin-7-O-β-D-glucopyranoside exhibited significant inhibitory activity against RLAR with IC50in the range of 7.34–23.99 μM. In addition, 3-(3,4-dihydroxyphenyl) propionic acid, neochlorogenic acid, and luteolin-3′-O-β-D-glucopyranoside exhibited the most potent inhibitory activity against formation of AGEs, with an IC50value of 115.07–184.06 μM, compared to the positive control aminoguanidine (820.44 μM). Based on these findings, SHL dietary supplements could be considered for the prevention and/or treatment of diabetes complication.

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Accumulation of fructosyl-lysine and advanced glycation end products in the kidney, retina and peripheral nerve of streptozotocin-induced diabetic rats

Biochemical Society Transactions ◽

10.1042/bst0311423 ◽

2003 ◽

Vol 31 (6) ◽

pp. 1423-1425 ◽

Cited By ~ 93

Author(s):

N. Karachalias ◽

R. Babaei-Jadidi ◽

N. Ahmed ◽

P.J. Thornalley

Keyword(s):

Peripheral Neuropathy ◽

Sciatic Nerve ◽

Peripheral Nerve ◽

Plasma Protein ◽

Advanced Glycation End Products ◽

Diabetic Rats ◽

Advanced Glycation ◽

Glycation End Products ◽

End Products ◽

Carboxymethyl Lysine

The accumulation of AGEs (advanced glycation end products) in diabetes mellitus has been implicated in the biochemical dysfunction associated with the chronic development of microvascular complications of diabetes – nephropathy, retinopathy and peripheral neuropathy. We investigated the concentrations of fructosyl-lysine and AGE residues in protein extracts of renal glomeruli, retina, peripheral nerve and plasma protein of streptozotocin-induced diabetic rats and normal healthy controls. Glycation adducts were determined by LC with tandem MS detection. In diabetic rats, the fructosyl-lysine concentration was increased markedly in glomeruli, retina, sciatic nerve and plasma protein. The concentrations of N∊-carboxymethyl-lysine and N∊-carboxyethyl-lysine were increased in glomeruli, sciatic nerve and plasma protein, and N∊-carboxymethyl-lysine also in the retina. Hydroimidazolone AGEs derived from glyoxal, methylglyoxal and 3-deoxylglucosone were major AGEs quantitatively. They were increased in the retina, nerve, glomeruli and plasma protein. AGE accumulation in renal glomeruli, retina, peripheral nerve and plasma proteins is consistent with a role for AGEs in the development of nephropathy, retinopathy and peripheral neuropathy in diabetes. High-dose therapy with thiamine and Benfotiamine suppressed the accumulation of AGEs, and is a novel approach to preventing the development of diabetic complications.

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N ε-(Carboxyethyl)lysine, a product of the chemical modification of proteins by methylglyoxal, increases with age in human lens proteins

Biochemical Journal ◽

10.1042/bj3240565 ◽

1997 ◽

Vol 324 (2) ◽

pp. 565-570 ◽

Cited By ~ 393

Author(s):

Mahtab U. AHMED ◽

Elisabeth BRINKMANN FRYE ◽

Thorsten P. DEGENHARDT ◽

Suzanne R. THORPE ◽

John W. BAYNES

Keyword(s):

Advanced Glycation End Products ◽

Human Lens ◽

Advanced Glycation ◽

Model Compounds ◽

Lens Proteins ◽

Glycation End Products ◽

End Products ◽

Lysine Residues ◽

Carboxymethyl Lysine ◽

Chemical Modification Of Proteins

Advanced glycation end-products and glycoxidation products, such as Nϵ-(carboxymethyl)lysine (CML) and pentosidine, accumulate in long-lived tissue proteins with age and are implicated in the aging of tissue proteins and in the development of pathology in diabetes, atherosclerosis and other diseases. In this paper we describe a new advanced glycation end-product, Nϵ-(carboxyethyl)lysine (CEL), which is formed during the reaction of methylglyoxal with lysine residues in model compounds and in the proteins RNase and collagen. CEL was also detected in human lens proteins at a concentration similar to that of CML, and increased with age in parallel with the concentration of CML. Although CEL was formed in highest yields during the reaction of methylglyoxal and triose phosphates with lysine and protein, it was also formed in reactions of pentoses, ascorbate and other sugars with lysine and RNase. We propose that levels of CML and CEL and their ratio to one another in tissue proteins and in urine will provide an index of glyoxal and methylglyoxal concentrations in tissues, alterations in glutathione homoeostasis and dicarbonyl metabolism in disease, and sources of advanced glycation end-products in tissue proteins in aging and disease.

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Inhibitory effect of leonurine on the formation of advanced glycation end products

Food & Function ◽

10.1039/c4fo00960f ◽

2015 ◽

Vol 6 (2) ◽

pp. 584-589 ◽

Cited By ~ 8

Author(s):

Lianqi Huang ◽

Xin Yang ◽

Anlin Peng ◽

Hui Wang ◽

Xiang Lei ◽

...

Keyword(s):

Advanced Glycation End Products ◽

Inhibitory Effect ◽

Advanced Glycation ◽

Glycation End Products ◽

End Products ◽

Carbonyl Species

Leonurine inhibits AGE formation through scavenging of the carbonyl species

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Inhibitory effect of polysaccharides from pumpkin on advanced glycation end-products formation and aldose reductase activity

Food Chemistry ◽

10.1016/j.foodchem.2011.07.064 ◽

2012 ◽

Vol 130 (4) ◽

pp. 821-825 ◽

Cited By ~ 26

Author(s):

Xuan Wang ◽

Liang-Shuan Zhang ◽

Lu-Lu Dong

Keyword(s):

Aldose Reductase ◽

Advanced Glycation End Products ◽

Reductase Activity ◽

Inhibitory Effect ◽

Advanced Glycation ◽

Glycation End Products ◽

End Products

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Leptin and advanced glycation end products receptor (RAGE) in tuberculosis patients

PLoS ONE ◽

10.1371/journal.pone.0254198 ◽

2021 ◽

Vol 16 (7) ◽

pp. e0254198

Author(s):

Tássia Kirchmann Lazzari ◽

Erika Cavalheiro ◽

Sandra Eugênia Coutinho ◽

Lívia Fontes da Silva ◽

Denise Rossato Silva

Keyword(s):

Weight Loss ◽

Advanced Glycation End Products ◽

Inverse Correlation ◽

Advanced Glycation ◽

Serum Leptin ◽

Soluble Rage ◽

Glycation End Products ◽

End Products ◽

Carboxymethyl Lysine ◽

Control Study

Introduction The pathogenesis of consumptive syndrome of tuberculosis (TB) is largely unknown. Leptin concentrations may be high because of the host’s inflammatory response, contributing to weight loss in patients with TB. The receptor for advanced glycation end products (RAGE) is also associated with weight loss in patients with TB and is related to enhanced mortality. The objective of this study was to evaluate the association between leptin and AGE/RAGE. Methods Case-control study. Leptin, AGE (carboxymethyl lysine, CML) and soluble RAGE (sRAGE) were measured from blood samples by ELISA. Results We included in the study 34 patients with TB and 34 controls. We found an inverse correlation between serum leptin levels and sRAGE, only in cases (r = -0.609, p < 0.0001). sRAGE levels were lower in patients with TB who died as compared with patients who survive (21.90 ± 4.24 pg/mL vs 66.14 ± 29.49 pg/mL; p = 0.045). Leptin levels were higher in patients with TB who died as compared with patients who survive (14.11 [7.48–14.11] ng/mL vs 3.08 [0.54–6.34] ng/mL; p = 0.028). Conclusions We identified lower sRAGE levels and higher leptin levels in patients with TB who died as compared with patients who survive. In addition, an inverse and significant correlation between serum leptin and sRAGE levels was demonstrated. Future studies, with a larger sample size and in different settings, including not only hospitalized patients, are needed to confirm these findings.

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