PCNA in Cervical Intraepithelial Neoplasia and Cervical Cancer: An Interaction Network Analysis of Differentially Expressed Genes

The investigation of differentially expressed genes (DEGs) and their interactome could provide valuable insights for the development of markers to optimize cervical intraepithelial neoplasia (CIN) screening and treatment. This study investigated patients with cervical disease to identify gene markers whose dysregulated expression and protein interaction interface were linked with CIN and cervical cancer (CC). Literature search of microarray datasets containing cervical epithelial samples was conducted in Gene Expression Omnibus and Pubmed/Medline from inception until March 2021. Retrieved DEGs were used to construct two protein-protein interaction (PPI) networks. Module DEGs that overlapped between CIN and CC samples, were ranked based on 11 topological algorithms. The highest-ranked hub gene was retrieved and its correlation with prognosis, tissue expression and tumor purity in patients with CC, was evaluated. Screening of the literature yielded 9 microarray datasets (GSE7803, GSE27678, GSE63514, GSE6791, GSE9750, GSE29570, GSE39001, GSE63678, GSE67522). Two PPI networks from CIN and CC samples were constructed and consisted of 1704 and 3748 DEGs along 21393 and 79828 interactions, respectively. Two gene clusters were retrieved in the CIN network and three in the CC network. Multi-algorithmic topological analysis revealed PCNA as the highest ranked hub gene between the two networks, both in terms of expression and interactions. Further analysis revealed that while PCNA was overexpressed in CC tissues, it was correlated with favorable prognosis (log-rank P=0.022, HR=0.58) and tumor purity (P=9.86 × 10-4, partial rho=0.197) in CC patients. This study identified that cervical PCNA exhibited multi-algorithmic topological significance among DEGs from CIN and CC samples. Overall, PCNA may serve as a potential gene marker of CIN progression. Experimental validation is necessary to examine its value in patients with cervical disease.

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The incidence of cervical intraepithelial neoplasia in antenatal women by screening: a study in a semi urban area in Telangana, India

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20172590 ◽

2017 ◽

Vol 6 (7) ◽

pp. 2887

Author(s):

Beera Neelima ◽

V. G. Vanamala

Keyword(s):

Cervical Cancer ◽

Cervical Intraepithelial Neoplasia ◽

Pap Smear ◽

Intraepithelial Neoplasia ◽

Low Grade ◽

Efficient Treatment ◽

Child Bearing ◽

Cervical Disease ◽

The Mean ◽

Epithelial Lesion

Background: Cervical intraepithelial neoplasia is a premalignant cervical disease which may lead to cervical cancer. The screening of the cervical cancer includes the screening of cervical cytology as well as testing for oncogenic human papilloma viruses (HPV) since most of the precursor lesions appear in the women of child bearing age. The purpose of the present study was to evaluate the incidence of cervical intraepithelial neoplasia in antenatal women by screening with papanicolaou smear.Methods: Macroscopic abnormalities such as cervicitis, polyps, erosions, nabothian cysts, hypertrophied cervix, growths and ulcers were noted. A cervical smear using Ayre’s spatula was taken from the ecto cervix and spread on to a labeled slide and immediately fixed.Results: Majority of the women were in the age group of 20-30 years in the study group. The overall mean age of the women in the study was around 25 years. The mean age at the time of menarche was around 14 years and at the time of marriage was approximately 22 years. On Pap smear, 55.2% of the 1000 cases were normal, with inflammation seen in 408 (40.8%) of the cases. 20 (2%) of the patients in our study had low grade squamous intra epithelial lesion, while 7 patients (0.2%) had high grade squamous intra epithelial lesion with two cases showing CIN II grading and 5 with CIN I.Conclusions: An early detection of the same can lead to a proper and efficient treatment.

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Effect of Ocimum on Cervical Intraepithelial Neoplasia (CIN) Pre Cervical Cancer

International Journal of Pharmaceutical Research ◽

10.31838/ijpr/2019.11.01.007 ◽

2019 ◽

Vol 11 (1) ◽

Keyword(s):

Cervical Cancer ◽

Cervical Intraepithelial Neoplasia ◽

Intraepithelial Neoplasia

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PECULIARITIES OF PAPILLOMAVIRUS GENOTYPING IN PATIENTS WITH CERVICAL INTRAEPITHELIAL NEOPLASIA

Ulyanovsk Medico-biological Journal ◽

10.34014/2227-1848-2020-3-104-111 ◽

2020 ◽

pp. 104-111

Author(s):

N.A. Shmakova ◽

G.N. Chistyakova ◽

I.N. Kononova ◽

I.I. Remizova

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Viral Load ◽

Cervical Intraepithelial Neoplasia ◽

Intraepithelial Neoplasia ◽

Squamous Intraepithelial Lesions ◽

Hpv 16 ◽

The World ◽

Intraepithelial Lesions ◽

Hpv 18

Recently, there has been a steady growth of cervical cancer all over the world, especially in Russia. Patients with cervical cancer have become much younger. At the same time, the human papillomavirus is not only the main factor in the neoplastic process, but it is also one of the most common sexually transmitted infections in the world. The aim of the paper is to assess the prevalence and characteristics of human papillomavirus genotypes in patients with cervical intraepithelial neoplasia. Materials and Methods. During the periodic screening we examined 213 women of a reproductive age with HPV infection. All patients underwent liquid-based cytology and human papillomavirus genotyping by polymerase chain reaction. Results. We revealed that the prevalence of cervical intraepithelial neoplasia among women with papillomavirus infection was 80.3 % (n=171). According to human papillomavirus genotyping, HPV 16 (38 %) and HPV 33 (32 %) prevailed. We also observed positive high correlation between high-grade squamous intraepithelial lesions (HSIL) and HPV 18 (r=+0.759, p=0.001), a negative mean correlation between HPV 45 and low-grade squamous intraepithelial lesions (LSIL) (r=-0.643, p=0.002). A cohort of patients with severe intraepithelial cervical lesions demonstrated high viral load rates. Conclusion. According to the results obtained, we established the dominance of HPV 16 and HPV 33 genotypes in cervical intraepithelial neoplasia. There were significant differences between HSIL and LSIL patients with HPV 18 and HPV 45. There was also a correlation between an increase in the viral load with the severity of the pathological process. Keywords: human papillomavirus, intraepithelial cervical neoplasms, cervical cancer. В последние годы в мире, особенно в России, наблюдается неуклонный рост и «омолаживание» рака шейки матки. При этом вирус папилломы человека является не только основным фактором прогрессирования неопластического процесса, но и одной из наиболее распространенных инфекций, предаваемых половым путем, в мире. Цель. Оценить распространенность и характеристику генотипов папилломавирусной инфекции у пациенток с цервикальными интраэпителиальными неоплазиями. Материалы и методы. Проведено обследование 213 пациенток репродуктивного возраста с ВПЧ-инфекцией, пришедших на профилактический осмотр. Всем женщинам было выполнено цитологическое исследование жидкостным методом и генотипирование вируса папилломы человека методом полимеразной цепной реакции. Результаты. Распространенность цервикальных интраэпителиальных неоплазий среди женщин с папилломавирусной инфекцией составила 80,3 % (171 пациентка). Согласно данным генотипирования вируса папилломы человека превалировал 16-й (38 %) и 33-й типы (32 %). Выявлена положительная высокая корреляционная связь между цервикальными неоплазиями высокой степени онкогенного риска (HSIL) и 18-м типом ВПЧ-инфекции (r=+0,759 при р=0,001), отрицательная средняя корреляционная связь 45-го типа ВПЧ с низкой степенью онкогенного риска (LSIL) (r=-0,643 при р=0,002). Продемонстрированы высокие показатели вирусной нагрузки в когорте пациенток с тяжелыми внутриэпителиальными цервикальными поражениями. Выводы. По результатам полученных данных установлено доминирование 16-го и 33-го генотипов ВПЧ при цервикальных интраэпителиальных неоплазиях с наличием значимых различий между пациентами с HSIL и LSIL в отношении 18-го и 45-го типов, а также связь роста уровня вирусной нагрузки с увеличением степени тяжести патологического процесса. Ключевые слова: вирус папилломы человека, интраэпителиальные новообразования шейки матки, рак шейки матки.

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Long noncoding RNA HOTAIR as a biomarker for the detection of Cervical Cancer and Cervical Intraepithelial Neoplasia

Indian Journal of Gynecologic Oncology ◽

10.1007/s40944-021-00552-6 ◽

2021 ◽

Vol 19 (4) ◽

Author(s):

Maryame Lamsisi ◽

Guorong Li ◽

Mustapha Benhessou ◽

Mohammed El Mzibri ◽

Amal Bouziyane ◽

...

Keyword(s):

Cervical Cancer ◽

Cervical Intraepithelial Neoplasia ◽

Long Noncoding Rna ◽

Noncoding Rna ◽

Intraepithelial Neoplasia

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CYP1A1 gene polymorphism as a risk factor for cervical intraepithelial neoplasia and invasive cervical cancer

Gynecologic Oncology ◽

10.1016/j.ygyno.2005.11.018 ◽

2006 ◽

Vol 101 (3) ◽

pp. 503-506 ◽

Cited By ~ 14

Author(s):

Cagatay Taskiran ◽

Dilek Aktas ◽

Nilufer Yigit-Celik ◽

Mehmet Alikasifoglu ◽

Kunter Yuce ◽

...

Keyword(s):

Cervical Cancer ◽

Risk Factor ◽

Gene Polymorphism ◽

Cervical Intraepithelial Neoplasia ◽

Invasive Cervical Cancer ◽

Intraepithelial Neoplasia ◽

Cyp1a1 Gene

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Thermal Coagulation Device for Treating Cervical Dysplasia

Surgical Innovation ◽

10.1177/1553350618819521 ◽

2018 ◽

Vol 26 (2) ◽

pp. 149-152 ◽

Cited By ~ 1

Author(s):

Ashley Langell ◽

Timothy Pickett ◽

Catherine Mangum ◽

Jennwood Chen ◽

John Langell

Keyword(s):

Cervical Cancer ◽

Cervical Intraepithelial Neoplasia ◽

Resource Constraints ◽

Low Cost ◽

Stakeholder Analysis ◽

Cervical Dysplasia ◽

Intraepithelial Neoplasia ◽

Cervical Cancer Prevention ◽

User Centered Design ◽

Thermal Coagulation

Background. Cervical cancer remains a leading cause of cancer-related deaths worldwide despite being a highly preventable disease. Nine out of every 10 deaths due to cervical cancer occur in developing regions with limited access to medical care and unique resource constraints. To address cervical cancer prevention within the confines of these unique limitations, our team of students and faculty advisors at the University of Utah’s Center for Medical Innovation developed a low-cost, portable technology that utilizes thermal coagulation, a form of heat ablation, to treat cervical intraepithelial neoplasia. Methods. A multidisciplinary team of students worked with clinical and industry advisors to develop a globally applicable treatment for cervical intraepithelial neoplasia through a systematic process of problem validation, stakeholder analysis, user-centered design, business plan development, and regulatory clearance. Results. Our efforts resulted in the development of a functional, self-contained, battery-operated prototype within 72 days, followed by Food and Drug Administration clearance of a finalized device within 18 months. Conclusion. Interdisciplinary university programs that leverage the capabilities of academic-industry partnerships can accelerate the development and commercialization of affordable medical technologies to solve critical global health issues.

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Human papillomavirus prevalence among women with cervical intraepithelial neoplasia III and invasive cervical cancer from Goiânia, Brazil

Memórias do Instituto Oswaldo Cruz ◽

10.1590/s0074-02762003000200003 ◽

2003 ◽

Vol 98 (2) ◽

pp. 181-184 ◽

Cited By ~ 26

Author(s):

SH Rabelo-Santos ◽

L Zeferino ◽

LL Villa ◽

JP Sobrinho ◽

RG Amaral ◽

...

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Cervical Intraepithelial Neoplasia ◽

Invasive Cervical Cancer ◽

Intraepithelial Neoplasia

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MTHFR/p53 Polymorphisms as Genetic Factors for Cervical Intraepithelial Neoplasia and Cervical Cancer in HPV-infected Mexican Women

The International Journal of Biological Markers ◽

10.5301/jbm.5000070 ◽

2014 ◽

Vol 29 (2) ◽

pp. 142-149 ◽

Cited By ~ 4

Author(s):

Lizbeth González-Herrera ◽

Patricia Rodríguez-Morales ◽

María del Refugio González-Losa ◽

Gerardo Pérez-Mendoza ◽

Jaqueline Canul-Canché ◽

...

Keyword(s):

Risk Factors ◽

Cervical Cancer ◽

Cervical Intraepithelial Neoplasia ◽

Genetic Risk ◽

Intraepithelial Neoplasia ◽

Genetic Risk Factors ◽

Mthfr Polymorphism ◽

Hpv Test ◽

P53 Polymorphism ◽

P53 Polymorphisms

We performed a case-control association study to evaluate the association between common polymorphisms in MTHFR (C677T and A1298C) and the Arg72Pro polymorphism in the p53 gene and the risk for cervical intraepithelial neoplasia (CIN) or invasive cervical cancer (ICC) in Mexican HPV-infected women. We included 131 women with diagnosis of CIN grade I-II and 78 with CIN III or ICC; as controls we also included 274 women with normal Pap smear and negative HPV test. Genotyping for MTHFR and p53 polymorphisms was performed by PCR-RFPLs. HPV was tested by Hybrid Capture II. Odds ratios and 95% confidence intervals were estimated. Genotype frequencies for the 3 studied polymorphisms were distributed according to the Hardy-Weinberg equilibrium. The A1298C-MTHFR polymorphism showed significant differences for the heterozygous AC genotype and the C allele, whereas the AA genotype and A allele resulted to be genetic risk factors for CIN or ICC (p<0.03). The Arg72Pro-p53 polymorphism showed for the genotypes Arg/Pro and Pro/Pro, and for the Pro allele, a significant association only to the risk for CIN (p<0.03). The MTHFR/p53 interaction showed that the genotype combinations AA/ArgArg and AA/ArgPro were associated, respectively, to the risk of ICC and CIN (p<0.05). This study suggests that the A1298C-MTHFR polymorphism contributes to the genetic risk for both CIN and ICC, whereas the Arg72Pro-p53 polymorphism only contributes to the risk for CIN. The MTHFR/p53 genetic combinations AA/ArgArg and AA/ArgPro are associated genetic risk factors for ICC and CIN in Mexican HPV-infected women.

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