scholarly journals Aberrant Development of Enteric Glial Cells in the Colon of Hirschsprung's Disease

2021 ◽  
Vol 9 ◽  
Author(s):  
Tingting Zhou ◽  
Wei Liu ◽  
Xiaofang Yu ◽  
Zengcai Cao ◽  
Weijing Mu ◽  
...  
Keyword(s):  
Glial Fibrillary Acidic Protein ◽  
Glial Cells ◽  
Calcium Binding ◽  
Hirschsprung's Disease ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Acidic Protein ◽  
S100 Calcium Binding Protein ◽  
Enteric Glial Cells ◽  
Protein B

Objective: The aim of this study was to explore the development of enteric glial cells (EGCs) in different segments of Hirschsprung's disease (HSCR).Methods: Colonic specimens from 35 children with HSCR were selected to analyze the relative expression of glial fibrillary acidic protein and S100 calcium-binding protein B using Western blotting and real-time fluorescence quantitative PCR. Immunofluorescence and immunohistochemical staining were performed to determine the distribution of myenteric EGCs and neuronal cells in different segments of HSCR.Results: There was a trend of diminished protein and mRNA expression of glial fibrillary acidic protein and S100 calcium-binding protein B from the proximal, dilated, and transitional segments to the aganglionic segment (p < 0.05). Immunofluorescence and immunohistochemistry showed that the EGCs in the aganglionic, transitional, and dilated colonic muscles were morphologically abnormal, which was consistent with the dysplasia of myenteric neurons.Conclusion: Aberrant development of myenteric EGCs was observed in the colon of HSCR, which may affect the survival of enteric neurons.

scholarly journals Influence of Simulated Pre-Hospital Transport, Time to Analysis, and Storage Temperature on S100 Calcium-Binding Protein B Values

2020 ◽  
Vol 37 (17) ◽  
pp. 1864-1869
Author(s):  
Julie Linding Kjerulff ◽  
Sophie-Charlott Seidenfaden ◽  
Niels Juul ◽  
Mette Fogh Møller ◽  
Anna-Marie Bloch Munster ◽  
...  
Keyword(s):  
Calcium Binding ◽  
Storage Temperature ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
Transport Time ◽  
B Values ◽  
S100 Calcium Binding Protein ◽  
Hospital Transport ◽  
And Storage ◽  
Protein B

scholarly journals Serum neuron-specific enolase and S100 calcium binding protein B biomarker levels do not improve diagnosis of acute stroke

2012 ◽  
Vol 42 (3) ◽  
pp. 199-204 ◽  
Author(s):  
S González-García ◽  
A González-Quevedo ◽  
M Peña-Sánchez ◽  
C Menéndez-Saínz ◽  
R Fernández-Carriera ◽  
...  
Keyword(s):  
Acute Stroke ◽  
Calcium Binding ◽  
Binding Protein ◽  
Neuron Specific Enolase ◽  
Calcium Binding Protein ◽  
S100 Calcium Binding Protein ◽  
Serum Neuron Specific Enolase ◽  
Protein B

Effects of Foot-Strike Patterns on Biomarkers S100 Calcium-Binding Protein B/Neuron-Specific Enolase in Running—A Pilot Study

2020 ◽  
Vol 15 (6) ◽  
pp. 900-902
Author(s):  
Jan Schroeder ◽  
Franziska Erthel ◽  
Karsten Hollander
Keyword(s):  
Calcium Binding ◽  
Binding Protein ◽  
Neuron Specific Enolase ◽  
Calcium Binding Protein ◽  
Significant Interaction Effect ◽  
Impact Forces ◽  
S100 Calcium Binding Protein ◽  
Foot Strike ◽  
Recreational Runners ◽  
Protein B

Purpose: Foot-strike patterns (FSP) in running are related to certain impact kinetics during ground contact. A rear-foot strike (RFS) has been associated with higher impact forces, whereas a forefoot strike (FFS) is assumed to minimize these impact forces. This study investigated the effects of running with RFS or FFS on impact-sensitive neuronal biomarkers S100 calcium-binding protein B (S100B), neuron-specific enolase (NSE), and lactate dehydrogenase (LDH). Methods: In this randomized crossover study, 18 healthy recreational runners (14 male, mean [SD] age 28.2 [4.7] y, body mass index 22.8 [1.9]) ran 2 separate 5-mile runs with RFS or FFS, respectively. Blood samples were taken before (pre), 30 min after (post 0), and 24 h after (post 24) the run. S100B, NSE, and LDH concentrations were determined and analyzed (3 × 2 data model). Results: S100B, NSE, and LDH concentrations increased from pre to post 0 and decreased within 24 h back to the baseline level, demonstrating a significant time effect (all P < .01, all ). No main effect for FSP or any significant interaction effect indicating FSP-specific differences between FFS or RFS was found (all P > .05, all ), except for LDH (P < .001, ). Conclusions: NSE, S100B, and LDH concentration increased in the short term after running and returned to normal in 24 h. FSP with varying impact forces does not influence the increase of these biomarkers. LDH findings favor peripheral origins, but mixed effects are possible. Thus, further research is needed to estimate the risk of mild traumatic encephalopathy.

845 Serum S100 Calcium-binding Protein B (S100B) Is Elevated in Severe Burn

2020 ◽  
Vol 41 (Supplement_1) ◽  
pp. S261-S262
Author(s):  
Jusong Song ◽  
Xiaofu Wang ◽  
Ryan M Huebinger ◽  
Ravi S Radhakrishnan ◽  
Steven E Wolf
Keyword(s):  
Myelin Basic Protein ◽  
Schwann Cells ◽  
Calcium Binding ◽  
Basic Protein ◽  
Binding Protein ◽  
Total Body Surface Area ◽  
Calcium Binding Protein ◽  
Severe Burn ◽  
S100 Calcium Binding Protein ◽  
Protein B

Abstract Introduction Severe burn causes a dramatic metabolic response associated with multi-organ damage. Mononeuropathy is also associated to muscle wasting after injury. Schwann cells are the glial cells that support neurons in the nervous system. S100 calcium-binding protein B (S100B) and Myelin basic protein (MBP) are biomarkers of Schwann cells. We hypothesize that severe burn affects these biomarkers, and is related to burn induced nerve impairment. The purpose of the study was to investigate biomarkers related to neural damage in response to severe burn. Methods Thirty-five Sprague Dawley rats received 40% total body surface area scald burn. Serum was collected at 6 hours, 1, 3, 7 and 14 days after injury. Six underwent sham burn as controls. Serum was analyzed for S100B and myelin basic protein using ELISA. One way ANOVA was applied for statistical analysis. Results The baseline of S100B level was 45.01±3.58 pg/ml in the sham group. S100B significantly increased to 99.42±9.13 pg/ml with no significant change at other time points. MBP serum level is 4.12± 0.13 ng/ml at baseline. MBP level did not change significantly in burn compared to sham. Conclusions In summary, S100B serum increased markedly after burn and returned to baseline thereafter. Burn did not change MBP levels. The findings could lead to further efforts in elucidating changes in neural function after burn. Applicability of Research to Practice The current study implies a novel biomarker candidate in response to severe burn related to nerve damage.

S100 calcium-binding protein B in older patients with depression treated with electroconvulsive therapy

2019 ◽  
Vol 110 ◽  
pp. 104414
Author(s):  
Angela Carlier ◽  
Kimberly Boers ◽  
Robert Veerhuis ◽  
Filip Bouckaert ◽  
Pascal Sienaert ◽  
...  
Keyword(s):  
Electroconvulsive Therapy ◽  
Calcium Binding ◽  
Older Patients ◽  
Binding Protein ◽  
Calcium Binding Protein ◽  
S100 Calcium Binding Protein ◽  
Protein B
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