scholarly journals Schizochytrium limacinum Supplementation in a Low Fish-Meal Diet Improved Immune Response and Intestinal Health of Juvenile Penaeus monodon

2020 ◽  
Vol 11 ◽  
Author(s):  
Shiwei Xie ◽  
Dan Wei ◽  
Beiping Tan ◽  
Yongjian Liu ◽  
Lixia Tian ◽  
...  
Keyword(s):  
Immune Response ◽  
Fish Meal ◽  
Penaeus Monodon ◽  
Intestinal Health ◽  
Schizochytrium Limacinum

Effects of fish origin probiotics on growth performance, immune response and intestinal health of shrimp ( Litopenaeus vannamei ) fed diets with fish meal partially replaced by soybean meal

2020 ◽  
Vol 26 (4) ◽  
pp. 1255-1265 ◽  
Author(s):  
Jiao‐Jin Zhang ◽  
Hong‐Ling Yang ◽  
Yang‐Yang Yan ◽  
Chun‐Xiao Zhang ◽  
Ji‐dan Ye ◽  
...  
Keyword(s):  
Immune Response ◽  
Growth Performance ◽  
Soybean Meal ◽  
Litopenaeus Vannamei ◽  
Fish Meal ◽  
Intestinal Health

scholarly journals Transversal gene expression panel to evaluate intestinal health in broiler chickens in different challenging conditions

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
L. Criado-Mesas ◽  
N. Abdelli ◽  
A. Noce ◽  
M. Farré ◽  
J. F. Pérez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Immune Response ◽  
Barrier Function ◽  
Nutrient Transport ◽  
Gene Expression Pattern ◽  
Vaccine Dose ◽  
Special Focus ◽  
Intestinal Health ◽  
Barrier Failure ◽  
Expression Of Genes

AbstractThere is a high interest on gut health in poultry with special focus on consequences of the intestinal diseases, such as coccidiosis and C. perfringens-induced necrotic enteritis (NE). We developed a custom gene expression panel, which could provide a snapshot of gene expression variation under challenging conditions. Ileum gene expression studies were performed through high throughput reverse transcription quantitative real-time polymerase chain reaction. A deep review on the bibliography was done and genes related to intestinal health were selected for barrier function, immune response, oxidation, digestive hormones, nutrient transport, and metabolism. The panel was firstly tested by using a nutritional/Clostridium perfringens model of intestinal barrier failure (induced using commercial reused litter and wheat-based diets without exogenous supplementation of enzymes) and the consistency of results was evaluated by another experiment under a coccidiosis challenge (orally gavaged with a commercial coccidiosis vaccine, 90× vaccine dose). Growth traits and intestinal morphological analysis were performed to check the gut barrier failure occurrence. Results of ileum gene expression showed a higher expression in genes involved in barrier function and nutrient transport in chickens raised in healthy conditions, while genes involved in immune response presented higher expression in C.perfringens-challenged birds. On the other hand, the Eimeria challenge also altered the expression of genes related to barrier function and metabolism, and increased the expression of genes related to immune response and oxidative stress. The panel developed in the current study gives us an overview of genes and pathways involved in broiler response to pathogen challenge. It also allows us to deep into the study of differences in gene expression pattern and magnitude of responses under either a coccidial vaccine or a NE.

scholarly journals ANALYSIS OF IMMUNE RESPONSES ON TRANSGENIC TIGER SHRIMP (Penaeus monodon) AGAINST PATHOGENIC BACTERIUM Vibrio harveyi

2014 ◽  
Vol 9 (1) ◽  
pp. 23
Author(s):  
Andi Parenrengi ◽  
Bunga Rante Tampangallo ◽  
Andi Tenriulo
Keyword(s):  
Immune Response ◽  
Immune Responses ◽  
Vibrio Harveyi ◽  
Gene Transfection ◽  
Penaeus Monodon ◽  
Pathogenic Bacterium ◽  
Shrimp Culture ◽  
Tiger Shrimp ◽  
Randomized Design ◽  
Study Results

Vibriosis is one of main diseases of the black tiger shrimp Penaeus monodon infected by pathogenic bioluminous bacterium Vibrio harveyi that can cause mass mortalities in shrimp culture. The bacteria can also trigger the disease white spot syndrome virus (WSSV). An effort to produce shrimp disease-resistant strains has been done through transgenesis technology with antiviral gene transfection. By this technology, it is expected an increase in the immune response of shrimp in a variety of diseasecausing pathogens. This study aimed to determine the immune responses (total haemocytes, haemocyte differentiation, and phenoloxydase activity) of transgenic tiger shrimp against pathogenic bacterium V. harveyi. Research using completely randomized design, which consists of two treatments and three replications. Test animals being used were transgenic and non-transgenic shrimp with size, weight 3.93±1.25 g and a total length of 7.59±0.87 cm. Treatments being tested were the injection of bacterium V. harveyi (density of 5x106 cfu/mL) of 0.1 mL/individual on transgenic (A) and non-transgenic shrimp (B). Immune response parameters such as total haemocytes, haemocyte differentiation, and phenoloxydase activity were observed on day 1, 3, and 6 days after challenging. Data were analyzed using t-test by SPSS software. The results showed that the total haemocyte of transgenic shrimp was not significantly different (P>0.05) from non-transgenic shrimp, but haemocyte differentiation and phenoloxydase activity were significantly different (P<0.05) especially on sixth days after being exposed to the bioluminescent bacteria. The study results implied that transgenic shrimp has a better immune response compared than non-transgenic shrimp.

A Spore‐Forming Probiotic Supplement Improves the Intestinal Immune Response and Protects the Intestinal Health During Recurrent Clostridioides difficile Colonization in Mice

2020 ◽  
Vol 44 (8) ◽  
pp. 1428-1438
Author(s):  
Fatemeh Ramezani Kapourchali ◽  
Bryan Glueck ◽  
Yingchun Han ◽  
David Shapiro ◽  
Clifton G. Fulmer ◽  
...  
Keyword(s):  
Immune Response ◽  
Intestinal Health ◽  
Clostridioides Difficile

Tilapia piscidin 4 (TP4) enhances immune response, antioxidant activity, intestinal health and protection against Streptococcus iniae infection in Nile tilapia

Aquaculture ◽  
2019 ◽  
Vol 513 ◽  
pp. 734451 ◽  
Author(s):  
Eman Zahran ◽  
Engy Risha ◽  
Samia Elbahnaswy ◽  
Hebatallah Ahmed Mahgoub ◽  
Amany Abd El-Moaty
Keyword(s):  
Immune Response ◽  
Antioxidant Activity ◽  
Nile Tilapia ◽  
Streptococcus Iniae ◽  
Intestinal Health

A probiotic Bacillus strain containing amorphous poly-beta-hydroxybutyrate (PHB) stimulates the innate immune response of Penaeus monodon postlarvae

2017 ◽  
Vol 68 ◽  
pp. 202-210 ◽  
Author(s):  
Joseph Leopoldo Q. Laranja ◽  
Edgar C. Amar ◽  
Gladys L. Ludevese-Pascual ◽  
Yufeng Niu ◽  
Mary Joy Geaga ◽  
...  
Keyword(s):  
Immune Response ◽  
Innate Immune Response ◽  
Penaeus Monodon ◽  
Innate Immune ◽  
Bacillus Strain ◽  
Beta Hydroxybutyrate

scholarly journals RESPONS IMUN UDANG WINDU Penaeus monodon TERHADAP VAKSIN dsRNA VP-24 PADA DOSIS BERBEDA

2018 ◽  
Vol 13 (1) ◽  
pp. 77
Author(s):  
Sri Redjeki Hesti Mulyaningrum ◽  
Andi Parenrengi ◽  
Bunga Rante Tampangallo ◽  
Ike Trismawanti
Keyword(s):  
Immune Response ◽  
Survival Rate ◽  
Saline Solution ◽  
Penaeus Monodon ◽  
Challenge Test ◽  
Injection Method ◽  
Tiger Shrimp ◽  
Total Hemocyte Count ◽  
Hemocyte Count

Peningkatan produksi udang windu Penaeus monodon terus diupayakan, salah satunya dengan peningkatan respons imun udang terhadap infeksi penyakit WSSV. Penelitian ini bertujuan untuk mengetahui respons imun udang terhadap pemberian vaksin dsRNA VP-24 pada berbagai dosis. Konstruksi vaksin dsRNA VP-24 dilakukan menggunakan Megascript kit dengan DNA genom VP-24 sebagai template. Vaksinasi dilakukan dengan metode injeksi pada udang windu yang berukuran rata-rata 15,88 ± 3,50 g; dosis vaksin yang diujikan adalah 0,02 µg; 0,2 µg; 2 µg; dan sebagai kontrol adalah udang yang tidak diberi vaksin. Penelitian terdiri atas empat perlakuan dosis vaksin dengan masing-masing dua ulangan dan dipelihara selama lima hari. Uji tantang dilakukan selama enam hari dengan menginjeksi virus WSSV dalam saline solution (1:3 v/v). Pengamatan terhadap sintasan udang windu dilakukan setiap hari, sedangkan penghitungan total hemocyte (THC) dan ProPO diamati pada hari I, III, dan VI setelah diinfeksi WSSV. Pada akhir pengujian dilakukan pengambilan jaringan hepatopankreas untuk analisis histopatologi. Analisis data dilakukan secara statistik dengan analisis ragam (ANOVA). Hasil yang diperoleh memperlihatkan bahwa injeksi vaksin dsRNA VP-24 dengan dosis 0,2 µg memiliki pengaruh yang signifikan terhadap sintasan dan respons imun udang windu (P<0,05). Vaksin dsRNA VP-24 dengan dosis 0,2 µg mampu memberikan sintasan udang windu P. monodon sebesar 65% dan meningkatkan respons imun udang dengan THC (1.550 x 10t sel/mL) dan ProPO (0,042 Abs).One of the efforts to increase the production of tiger shrimp Penaeus monodon is increasing the immune response against WSSV disease. This study aims to evaluate shrimp immune response to dsRNA VP-24 vaccination at various doses. The construction of dsRNA VP-24 vaccine was performed using Megascript kit with the VP-24 DNA genome as a template. The vaccination was done by injection method on shrimp sized 15.88 ± 3.50 g. The tested vaccine doses (treatments) were 0.02 ¼g; 0.2 ¼g; 2 ¼g; and unvaccinated shrimp as the control. The study consisted of four treatments of vaccine doses with two replicates for each treatment. The challenge test was performed by injecting the WSSV virus in saline solution (1:3 v/v). The observation on shrimp survival rate was done daily, while the total hemocyte count (THC) and ProPO observation were performed on the 1st day, 3rd day, and 6th day after WSSV infection. At the end of the experiment, samplings of hepatopancreas for analysis were performed. Data were statistically analyzed using ANOVA. The present study indicated that the injection of 0.2 ¼ g dsRNA VP-24 vaccine had a significant effect on the survival rate and immune response of shrimp (P<0.05). The dose of 0.2 ¼g dsRNA VP-24 had resulted in 65% of survival rate and increased immune response of P. monodon with THC (1,550 x 10t cell/mL) and ProPO (0.042 Abs).

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