Proteomic Characterization of Dry Blood Spots of Healthy Women During Simulation the Microgravity Effects Using Dry Immersion

ABSTRACTDeep characterization, even by next-generation sequencing, of the vaginal microbiota in healthy women or posttreatment bacterial vaginosis patients is limited by the dominance of lactobacilli. To improve detection, we offer two approaches: quantitative PCR (qPCR) using phylogenetic branch-inclusive primers and sequencing of broad-spectrum amplicons generated with oligomers that block amplification of lactobacilli.

Download Full-text

Steroid profiling by UHPLC-MS/MS in dried blood spots collected from healthy women with and without testosterone gel administration

Journal of Pharmaceutical and Biomedical Analysis ◽

10.1016/j.jpba.2021.114280 ◽

2021 ◽

pp. 114280

Author(s):

Olivier Salamin ◽

Raul Nicoli ◽

Cheng Xu ◽

Julien Boccard ◽

Serge Rudaz ◽

...

Keyword(s):

Dried Blood Spots ◽

Blood Spots ◽

Healthy Women ◽

Steroid Profiling ◽

Testosterone Gel

Download Full-text

Characterization of profiles of salivary progesterone concentrations during the luteal phase of fertile and subfertile women

Journal of Endocrinology ◽

10.1677/joe.0.1040441 ◽

1985 ◽

Vol 104 (3) ◽

pp. 441-446 ◽

Cited By ~ 14

Author(s):

R. F. Walker ◽

D. W. Wilson ◽

P. L. Truran ◽

G. F. Read ◽

G. Richards ◽

...

Keyword(s):

Statistical Analysis ◽

Menstrual Cycle ◽

Computer Program ◽

Luteal Phase ◽

Cumulative Sum ◽

Healthy Women ◽

History Of

ABSTRACT Salivary progesterone concentrations were measured in daily samples collected between 08.00 and 09.00 h throughout the menstrual cycle of women with a history of fertility. The luteal-phase salivary progesterone profiles in these normally menstruating, healthy women were characterized using a computer program based on a cumulative sum procedure. This method of statistical analysis led to the development of a 'progesterone boundary diagram', the inner and outer domains of which distinguished between the profiles of salivary progesterone considered compatible with fertility, and those observed in subfertile women attending an infertility clinic. J. Endocr. (1985) 104, 441–446

Download Full-text

Application of Dried Blood Spots and Serum Samples for the Determination of Vitamin D Metabolites in the Group of Healthy Women and with Hashimoto’s Thyroiditis

Chromatographia ◽

10.1007/s10337-021-04047-6 ◽

2021 ◽

Author(s):

R. Rola ◽

E. Trusewicz ◽

T. Bieńkowski ◽

S. Studzińska

Keyword(s):

Vitamin D ◽

Hashimoto’S Thyroiditis ◽

Good Alternative ◽

Dried Blood Spots ◽

Hashimoto's Thyroiditis ◽

Vitamin D Metabolites ◽

Serum Samples ◽

Blood Spots ◽

Healthy Women

Abstract Purpose The relationship between Hashimoto's thyroiditis and vitamin D concentration was already presented in many studies. The aim of this study was to analyze the differences in the concentration of vitamin D metabolites between healthy women and women with Hashimoto's thyroiditis (HT). Methods The quantitative analysis of five vitamin D metabolites was carried out using liquid chromatography coupled with tandem mass spectrometry. The analyzed materials were serum and dried blood spots (DBS). The results obtained for the two materials were also compared. Results No statistically significant differences were found in the mean concentration of the 25(OH)D3, 25(OH)D2, 24,25(OH)2D3, 1,25(OH)2D3 metabolites between the test and the control groups. However, a strong correlation was found between the 25(OH)D3 and 24,25(OH)2D3 metabolites. Conclusion The study showed that healthy women and women with Hashimoto's disease had similar concentration of vitamin D metabolites. Research also proved that DBS is a good alternative to serum. The differences in 25(OH)D concentration were not statistically significant (17.0 and 15.5 ng mL−1 for serum and DBS, respectively). DBS can be successfully used in research on a large group of people, since the process of material collection, as well as sample preparation, is fast and simple. It is also easy to transport and store, and requires small volume of blood.

Download Full-text