scholarly journals Nodulation by Sinorhizobium meliloti originated from a mining soil alleviates Cd toxicity and increases Cd-phytoextraction in Medicago sativa L.

2015 ◽  
Vol 6 ◽  
Author(s):  
Tahar Ghnaya ◽  
Majda Mnassri ◽  
Rim Ghabriche ◽  
Mariem Wali ◽  
Charlotte Poschenrieder ◽  
...  
Keyword(s):  
Medicago Sativa ◽  
Sinorhizobium Meliloti ◽  
Cd Toxicity ◽  
Mining Soil ◽  
Medicago Sativa L

Evaluation of Rhizobium inoculant formulations for alfalfa yield and N fixation

2007 ◽  
Vol 87 (2) ◽  
pp. 267-272 ◽  
Author(s):  
J. Diane Knight
Keyword(s):  
High Risk ◽  
Medicago Sativa ◽  
Sinorhizobium Meliloti ◽  
The Other ◽  
N Fixation ◽  
Biological N Fixation ◽  
Small Seed ◽  
Alfalfa Seed ◽  
Medicago Sativa L

Because of its small seed size, alfalfa (Medicago sativa L.) typically is seeded at a shallow depth, putting Rhizobium inoculated onto the seed coat at a high risk of desiccation. Granular inoculants may provide a superior delivery formulation for Rhizobium because the inoculant can be placed deeper in the soil than the seed, where it is protected from desiccation. Sinorhizobium meliloti cv. Beaver delivered as (1) pre-inoculated alfalfa seed from the inoculant manufacturer, (2) commercial peat-based, self-sticking inoculant applied on-site, and (3) granular inoculant placed with the seed or (4) banded below and to the side of the seed was evaluated against uninoculated alfalfa controls at three field sites in Saskatchewan. Overall, alfalfa inoculated with the granular formulation placed below and to the side of the alfalfa seed was among the highest biomass producer in the establishment year, although it did not exhibit superior nodulation or biological N fixation compared with the other treatments. Any advantage conferred by the granular inoculant in the establishment year did not persist through the first and second production years. Under the conditions of this study, the delivery formulation had no long-term affect on alfalfa productivity, nodulation and biological N fixation. Furthermore, none of the inoculant treatments were consistently superior to the uninoculated controls. Key words: Alfalfa, granular inoculant, peat inoculant, pre-inoculated seed, Medicago sativa L., Sinorhizobium meliloti

scholarly journals Effect of Salinity on Nodulation, Glutamine Synthetase and Glutamate Synthase Activity in Nodules of Alfalfa (Medicago sativa L.)

2015 ◽  
Vol 48 (4) ◽  
pp. 61-70
Author(s):  
O. Younesi ◽  
A. Moradi
Keyword(s):  
Glutamine Synthetase ◽  
Medicago Sativa ◽  
Sinorhizobium Meliloti ◽  
Glutamate Synthase ◽  
Dry Weight ◽  
Ammonium Assimilation ◽  
Solution Culture ◽  
Saline Stress ◽  
Medicago Sativa L ◽  
Growth Analyses

Abstract Bami cultivar of alfalfa (Medicago sativa) was inoculated with salt-tolerant Sinorhizobium meliloti in solution culture with different salt concentrations (0, 50, 75 and 100 mmoles 1-1NaCl) added immediately at the time of inoculation. The results indicated that S. meliloti formed an infective and effective symbiosis with alfalfa under saline and nonsaline conditions. Salinity significantly decreased shoot and root dry weight, nodule weight and mean nodule weight. Roots were more sensitive than shoots, and N2 fixation was more sensitive to salinity than was plant growth. Analyses of ammonium assimilating enzymes in the nodule showed that glutamine synthetase appeared to be more tolerant to salinity than glutamate synthase, and that it limits ammonium assimilation under saline stress.

Response of alfalfa (Medicago sativa L.) to single and mixed inoculation with phosphate-solubilizing bacteria and Sinorhizobium meliloti

2009 ◽  
Vol 46 (2) ◽  
pp. 185-190 ◽  
Author(s):  
Lorena Belén Guiñazú ◽  
Javier Alberto Andrés ◽  
María Florencia Del Papa ◽  
Mariano Pistorio ◽  
Susana Beatriz Rosas
Keyword(s):  
Medicago Sativa ◽  
Sinorhizobium Meliloti ◽  
Phosphate Solubilizing Bacteria ◽  
Medicago Sativa L ◽  
Phosphate Solubilizing

Populations of Sinorhizobium meliloti congregate in the 30 - 60 cm section of the soil profile in a stand of dryland lucerne (Medicago sativa): is this where lucerne fixes its nitrogen?

2005 ◽  
Vol 45 (3) ◽  
pp. 225 ◽  
Author(s):  
C. M. Evans ◽  
N. A. Fettell ◽  
J. Brockwell
Keyword(s):  
Medicago Sativa ◽  
Soil Profile ◽  
Sinorhizobium Meliloti ◽  
New South ◽  
Red Clay ◽  
Plant Infection ◽  
South Wales ◽  
Below Ground ◽  
Medicago Sativa L ◽  
Very High

Lucerne (Medicago sativa L.) cv. Hunterfield was inoculated with Sinorhizobium meliloti and sown in a red clay loam at Condobolin, New South Wales. The soil had been limed at 6 different rates 8 years previously. In 2001, when the stands were 3 years old, the populations of S. meliloti were enumerated, with a plant-infection test, in 4 sections of the soil profile (i.e. 0–15 cm, 15–30 cm, 30–60 cm and >60 cm). The numbers of rhizobia in the soil were very high (120 000 rhizobia/g) in the 30–60 cm section. In sharp contrast, much lower numbers (0–15 cm, 75 rhizobia/g; 15–30 cm, 190 rhizobia/g; >60 cm, 287 rhizobia/g) were detected in the other sections of profile. Liming had no effect on size of S. meliloti population. It was concluded that, under the conditions of the experiment, it was reasonable to assume that lucerne nodulated most abundantly between 30–60 cm below ground. This zone may represent the location of maximum nitrogen fixation. Some implications of the conclusions are discussed.

scholarly journals Effects of different strains of Sinorhizobium meliloti on alfalfa (Medicago sativa L.) biomass yield

2007 ◽  
Vol 23 (5-6-1) ◽  
pp. 601-607 ◽  
Author(s):  
D. Delic ◽  
O. Stajkovic ◽  
B. Milicic ◽  
Dj. Kuzmanovic ◽  
N. Rasuluc ◽  
...  
Keyword(s):  
Medicago Sativa ◽  
Crude Protein ◽  
Dry Matter ◽  
Sinorhizobium Meliloti ◽  
Mineral Fertilization ◽  
The Third ◽  
Medicago Sativa L ◽  
Second Year ◽  
Different Strains ◽  
Physical And Chemical

The effects of highly effective strains of Sinorhizobium meliloti on biomass and crude protein yields of alfalfa (Medicago sativa L.) were investigated in a three-year field experiment focusing on the advantage of alfalfa inoculation with N microbiological fertilizer over N mineral fertilization on chernozem soil. Strains 236 and 234 were found in this experiment to have significant effect on the yields of dry matter and proteins, by comparison to untreated control plants. In the third year of utilization, dry matter yield of alfalfa plants inoculated with strains 236 and 234 increased around 31% in the first cutting and 53% and 65% in the second one, compared to control plants. Strain 236 was found to increase the yield of crude proteins by 29.7% (second cutting) and 39.6% (third cutting) in the second year, and 27.5% (first cutting) and 50.3% (second cutting) in the third year of utilization, while strain 234 increased significantly the content of proteins in the third cutting of the second year (27%) and in the third year of utilization (51% in the first and 53% in the second cutting). The results indicate that effective strains of S. meliloti may be applied as N microbiological fertilizers on soils with optimal physical and chemical characteristics and autochthonous populations that may not have satisfactory effectiveness in nitrogen fixation.

Estimación de biomasa aérea de forrajes de invierno bajo riego a través de un dron

2019 ◽  
Vol 12 (4) ◽  
Author(s):  
Francisco Gavi Reyes ◽  
César Botello-Aguillón ◽  
Leonardo Tijerina-Chávez ◽  
Arturo Galvis-Spíndola ◽  
Rodrigo Roblero-Hidalgo
Keyword(s):  
Medicago Sativa ◽  
Avena Sativa ◽  
Medicago Sativa L

E Objetivo: Desarrollar un procedimiento para estimar biomasa con imágenes digitales captadas desde un dron y modelación 3D (ID-Dron-3D) aplicable en alfalfa (Medicago sativa L.) y avena forrajera (Avena sativa L.). Diseño/metodología/aproximación: Con una cámara digital acoplada al dron se obtuvieron imágenes antes de la cosecha de los cultivos, que fueron procesadas con software para luego estimar volumen de biomasa. En cada cultivo se midió altura de la planta y área cosechada, volumen aparente y real de biomasa, y peso de biomasa fresca y seca. Resultados: Con base en el análisis de regresión se obtuvieron modelos lineales a una p<0.05 para predecir: biomasa fresca en avena (R2=0.70) y alfalfa (R2 =0.47); y biomasa seca en avena (R2=0.78) y en alfalfa (R2=0.31) mediante ID-Dron-3D. Limitaciones del estudio/implicaciones: Considerando las R2 de los modelos obtenidos, los resultados en la avena forrajera fueron mejores, respecto a los detectados en alfalfa, lo cual se puede deber a la mayor variabilidad de la cobertura vegetal, ya que, en algunas unidades de muestreo, las plantas de alfalfa no cubrían completamente el suelo. Hallazgos/conclusiones: El rendimiento de biomasa fresca y seca de ambos cultivos se correlacionó significativamente con su respectivo volumen aparente estimado con imágenes digitales tomadas desde un dron y su procesamiento 3D (ID-Dron-3D).

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