scholarly journals Deciphering Genomic Regions for High Grain Iron and Zinc Content Using Association Mapping in Pearl Millet

2017 ◽  
Vol 8 ◽  
Author(s):  
N. Anuradha ◽  
C. Tara Satyavathi ◽  
C. Bharadwaj ◽  
T. Nepolean ◽  
S. Mukesh Sankar ◽  
...  
2017 ◽  
Vol 77 (1) ◽  
pp. 65 ◽  
Author(s):  
N. Anuradha ◽  
C. Tara Satyavathi ◽  
M. C. Meena ◽  
S. Mukesh Sankar ◽  
C. Bharadwaj ◽  
...  

2012 ◽  
Vol 47 (8) ◽  
pp. 1660-1668 ◽  
Author(s):  
Fatoumata Hama ◽  
Christèle Icard-Vernière ◽  
Jean-Pierre Guyot ◽  
Isabelle Rochette ◽  
Bréhima Diawara ◽  
...  

2016 ◽  
Vol 7 ◽  
Author(s):  
Sushil Kumar ◽  
Charles T. Hash ◽  
Nepolean Thirunavukkarasu ◽  
Govind Singh ◽  
Vengaldas Rajaram ◽  
...  

2016 ◽  
Vol 15 (5) ◽  
pp. 438-452 ◽  
Author(s):  
H. D. Upadhyaya ◽  
K. N. Reddy ◽  
M. Irshad Ahmed ◽  
Senthil Ramachandran ◽  
Vinod Kumar ◽  
...  

AbstractThe world collection of pearl millet at ICRISAT genebank includes 19,696 landraces. Passport and characterization data of 2,929 accessions belonging to 89 named landraces originating in 15 countries of Africa was used to study the adoption pattern and genetic potential. Out of 89 named landraces under study, 71 were grown in one country, 11 in two countries, six in three countries and one in four countries. Latitude and prevailing climate at collection sites were found as the important determinants of cultivation pattern of landraces. A hierarchical cluster analysis using 12 agronomic traits resulted in five clusters. Cluster 1 for late flowering, short height in rainy season, high tillering and thin panicles; cluster 2 for early flowering; cluster 3 for stout panicles in both the seasons and larger seeds and cluster 5 for longer panicles in both seasons, were found as promising sources. IP 8957, IP 8958, IP 8964 of Iniadi landrace for short height, downy mildew and rust resistance and high seed iron and zinc contents; IP 17521 of Gnali (106.9 ppm) and IP 11523 of Idiyouwe (106.5 ppm) for high seed iron content; IP 17518 of Gnali (79.1 ppm) and IP 11535 of Iniadi (78.4 ppm) for high seed zinc content were the important sources. All accessions of Raa for high seed protein content (>15%) and those of Enele for drought tolerance, were found to be promising sources. Further evaluation of promising sources identified in this study is needed for enhanced utilization of germplasm in pearl millet improvement.


2017 ◽  
Vol 40 (04) ◽  
Author(s):  
Vijayata Singh ◽  
R. K. Yadav ◽  
N. R. Yadav ◽  
Rajesh Yadav ◽  
R. S. Malik ◽  
...  

Among the legumes, mungbean has highest digestive protein but low micronutrient content like iron and zinc. Biofortification of mungbean has been undertaken to reduce micronutrient malnutrition. The objectives of this study were to identify QTLs for seed Fe and Zn content in F6 recombinant inbred line (RIL) population (ML776 x Sattya). A large genetic variation and transgressive segregation in RILs were observed for Fe and Zn content. Linkage map was developed which spanned 2919.7cM distance. 17 QTLs (2 for iron and 15 for zinc content) were mapped on four linkage groups; LG 4, LG 6, LG 7 and LG 11 in mungbean. The genomic regions qZn-4-3 and qFe-4-1 on chromosome 4 between PVBR82-BM210 markers; qZn-11-2 and qFe-11-1 on chromosome11 between BM141-BM184 markers, were co-located on the same chromosomal regions for Zn or Fe concentration, which probably were closely linked to each other, or were the same pleiotropic QTLs. The SSR markers associated with QTLs for both high iron and zinc content would also be useful in marker assisted breeding for biofortification in mungbean.


2007 ◽  
Vol 126 (2) ◽  
pp. 182-185 ◽  
Author(s):  
G. Velu ◽  
K. N. Rai ◽  
V. Muralidharan ◽  
V. N. Kulkarni ◽  
T. Longvah ◽  
...  

Genes ◽  
2018 ◽  
Vol 9 (5) ◽  
pp. 248 ◽  
Author(s):  
Sushil Kumar ◽  
Charles Hash ◽  
Thirunavukkarasu Nepolean ◽  
Mahesh Mahendrakar ◽  
Chellapilla Satyavathi ◽  
...  

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Peter Anabire Asungre ◽  
Richard Akromah ◽  
Alexander Wireko Kena ◽  
Prakash Gangashetty

Twenty-two open-pollinated varieties (OPVs) of pearl millet (Pennisetum glaucum) genotypes were tested in two locations for three seasons in Ghana to estimate the magnitude of genetic variability, heritability, and stability for grain yield and related traits and grain micronutrients among the varieties. General analysis of variance within and across locations and years revealed very highly significant variability ( p < 0.01 ) among the genotypes. The additive main effects and multiplicative interaction (AMMI) analyses revealed significant genotype × environment interaction (GEI) that influenced the relative ranking of genotypes across the environments. Genotypic variance ( σ 2 g ) contributed a greater proportion of the phenotypic variance (σ2p) for plant height (530.31) and grain Fe content (34.72). Broad-sense heritability ( h b s 2 ) varied widely from 24.82% for grain yield to 77.53% in days to flower. Phenotypic coefficient of variation (PCV) was higher than genotypic coefficient of variation (GCV) for all traits, indicating strong play of environment on trait expressions. 11 out of the 22 OPVs were stable for grain yield and micronutrients across environments for the three-year period and included GB 8735 and ICMV 221 Wbr and SOSAT-C88.


2021 ◽  
Author(s):  
C. Tara Satyavathi ◽  
Rukam S. Tomar ◽  
Supriya Ambawat ◽  
Jasminkumar Kheni ◽  
Shital M. Padhiyar ◽  
...  

Abstract Pearl millet is an important staple food crop of poor people which is rich in micronutrients like iron and zinc and amenable for focused breeding for these micronutrients along with high yield. Transcriptome sequencing using ION S5 Next Generation Sequencer generated 43.5 million sequence reads resulting in 83,721 transcripts with N50 of 597 bp and 84.35% of transcripts matched with the pearl millet genome assembly. The genotypes having high Fe and Zn showed differential gene expression during different stages. Of which, 155 were up-regulated and 251were down-regulated while during flowering stage and milking stage 349 and 378 transcripts were differentially expressed, respectively. Gene annotation and GO term showed the presence of transcripts involved in metabolic activities associated with uptake and transport of iron and zinc. In the present study, the 83,721 transcripts were also examined for identification of SSRs. A total of 4,327 SSRs were identified with dominance of tri-nucleotide SSRs in comparison to di-nucleotide SSRs. These EST-SSRs can be used in molecular breeding, genetic diversity analysis and determination of heterozygosity of the allelic loci. Information generated will help in gaining insights into iron and zinc metabolism and develop genotypes with high yield, grain iron and zinc content.


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