Identification of genomic Regions/genes for high iron and zinc content and cross transferability of SSR markers in mungbean (Vigna radiata L.)

2017 ◽  
Vol 40 (04) ◽  
Author(s):  
Vijayata Singh ◽  
R. K. Yadav ◽  
N. R. Yadav ◽  
Rajesh Yadav ◽  
R. S. Malik ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Zinc Content ◽  
Transgressive Segregation ◽  
Chromosome 4 ◽  
Marker Assisted Breeding ◽  
High Iron ◽  
Zn Content ◽  
Iron And Zinc ◽  
Micronutrient Malnutrition ◽  
Genomic Regions

Among the legumes, mungbean has highest digestive protein but low micronutrient content like iron and zinc. Biofortification of mungbean has been undertaken to reduce micronutrient malnutrition. The objectives of this study were to identify QTLs for seed Fe and Zn content in F6 recombinant inbred line (RIL) population (ML776 x Sattya). A large genetic variation and transgressive segregation in RILs were observed for Fe and Zn content. Linkage map was developed which spanned 2919.7cM distance. 17 QTLs (2 for iron and 15 for zinc content) were mapped on four linkage groups; LG 4, LG 6, LG 7 and LG 11 in mungbean. The genomic regions qZn-4-3 and qFe-4-1 on chromosome 4 between PVBR82-BM210 markers; qZn-11-2 and qFe-11-1 on chromosome11 between BM141-BM184 markers, were co-located on the same chromosomal regions for Zn or Fe concentration, which probably were closely linked to each other, or were the same pleiotropic QTLs. The SSR markers associated with QTLs for both high iron and zinc content would also be useful in marker assisted breeding for biofortification in mungbean.

scholarly journals Promotor Anchored - RAPD Analysis of Foxtail Millet (Setaria italica L.) Accessions Selected For High Iron and Zinc Content

2021 ◽  
pp. 36-48
Author(s):  
Dhiraj Gangtire ◽  
Nakul D. Magar ◽  
Vaibhav Khelurkar ◽  
Mangesh P. Moharil ◽  
P. V. Jadhav ◽  
...  
Keyword(s):  
Polymorphic Information Content ◽  
Foxtail Millet ◽  
Zinc Content ◽  
Biochemical Properties ◽  
Setaria Italica ◽  
High Genetic Diversity ◽  
High Iron ◽  
Average Value ◽  
Zn Content ◽  
Iron And Zinc

Foxtail millet (Setaria italica L.) is nutri-cereal crop having it is rich in β-carotene, vitamin B-complex and micronutrients like minerals. In the present research, we have studied biochemical properties and molecular profiling to identify the core set of foxtail millet (Setaria italic L.) accessions for high Iron (Fe) and Zinc (Zn) content. Total seventy-nine accessions and selected mutants variety PS4 of foxtail millet were used. The biochemical investigation revealed that accessions M2-106, IC120407, M3-61/HB-13, and IC120255 consist of high iron and zinc content. The genetic variability among the genotypes was revealed by 28 Promoter Anchored Amplified Polymorphic (PAAP-RAPD) primers of which OPE9+GC1, OPE9+CA1, UBC001+CA1, UBC001+TA1, UBC693+G1 showed 100% polymorphism, whereas UBC693+GC1 and OPE7+G1 showed 88% and 80% polymorphism, respectively, with an average of 45.95 % polymorphism. Total alleles per locus were 3.31, whereas, the average number of monomorphic and polymorphic alleles were 1.72 and 1.56, respectively. The extent of polymorphic information content (PIC) of PAAP-RAPD loci ranged from 0.5 to 0.87 with an average value of 0.41. For PAAP-RAPD, the maximum PIC value was observed in marker UBC693+GC1 (0.87 %) and the minimum were OPE5+CA1 and OPE9+CA1 (0.5 %). Molecular characterizations result showing highest similarity (0.932) between accessions Shrilakshmi and Prasad, whereas, the lowest similarity coefficient was observed between IC120255 and M3-75/AM-1 (0.697) with PS4.UPGMA dendrogram grouped the foxtail millet accessions in five clusters which marked high diversity in M3-61/HB-13 and M3-75/AM-1. It implies that PAAP-RAPD markers are significantly screened in the foxtail millet accessions and have enumerated high genetic diversity.

scholarly journals Diversity of Iron and Zinc Content in Bananas from East and Central Africa

HortScience ◽  
2016 ◽  
Vol 51 (4) ◽  
pp. 320-324
Author(s):  
Michael Pillay ◽  
Robert Fungo
Keyword(s):  
Central Africa ◽  
Germplasm Collection ◽  
Zinc Content ◽  
Atomic Absorption Spectrophotometry ◽  
Absorption Spectrophotometry ◽  
Fiber Protein ◽  
Fe Content ◽  
Zn Content ◽  
Wide Variability ◽  
Iron And Zinc

Bananas and plantains (Musa sp.) are major staple foods in many developing countries of the world. Although bananas are rich in carbohydrate, fiber, protein, fat, and vitamins A, C, and B6 they are largely deficient of iron (Fe), iodine, and zinc (Zn). A small increase in the micronutrient content of bananas could play a major role in combating disorders that are due to deficiency of mineral micronutrients such as Fe and Zn. The objective of this study was to determine the Fe and Zn content of 47 banana genotypes from a germplasm collection in Uganda using atomic absorption spectrophotometry. The Fe and Zn content showed wide variability and highly significant differences (P < 0.001) within and among the different banana categories selected for this study. The highest average Fe content (1.42 mg/100 g) was found in ‘Saba’ (ABB) while the least Fe content (0.06 mg/100 g) was found in ‘Kikundi’ (AAA). The highest average Zn content (1.21 mg/100 g) among the analyzed accessions was found in ‘Kivuvu’ (ABB) while Zn was not detectable in both ‘Kabucuragye’ (AAA) and ‘Grand Naine’ (AAA). Considering these figures, there is a greater than 20-fold variation in the Fe and Zn levels of the banana genotypes used this study suggesting that genetic improvement of genotypes for enhanced micronutrient levels may be achieved by breeding.

scholarly journals Mapping Quantitative Trait Loci Controlling High Iron and Zinc Content in Self and Open Pollinated Grains of Pearl Millet [Pennisetum glaucum (L.) R. Br.]

2016 ◽  
Vol 7 ◽  
Author(s):  
Sushil Kumar ◽  
Charles T. Hash ◽  
Nepolean Thirunavukkarasu ◽  
Govind Singh ◽  
Vengaldas Rajaram ◽  
...  
Keyword(s):  
Quantitative Trait Loci ◽  
Pearl Millet ◽  
Quantitative Trait ◽  
Pennisetum Glaucum ◽  
Zinc Content ◽  
High Iron ◽  
Trait Loci ◽  
Iron And Zinc

scholarly journals Assessment of Gene Action for Grain Micronutrient Content, Yield and Yield Contributing Traits in Rice (Oryza sativa L.)

2020 ◽  
pp. 56-63
Author(s):  
Ashutosh Kumar ◽  
Avinash Kumar ◽  
N. K. Singh ◽  
Rajesh Kumar ◽  
. Nilanjaya ◽  
...  
Keyword(s):  
Gene Action ◽  
Oryza Sativa L ◽  
Zinc Content ◽  
Dominance Model ◽  
Gene Effects ◽  
Gene Effect ◽  
Biotechnological Approach ◽  
Zn Content ◽  
H Gene ◽  
Iron And Zinc

Biofortification of food crops using conventional breeding or biotechnological approach is gaining momentum to alleviate micronutrient malnutrition.  Rice is a nice choice for biofortification of grain iron and zinc content as this is a cheap and chief staple food for millions of peoples world-wide particularly the poor. In present study, generation mean analysis was done to estimate the nature and magnitude of gene effects for grain iron and zinc content in rice cross Khusisoi-RI-Sareku × IR 91175-27-1-3-1-3.  Scaling test and Joint scaling test indicated the influence of epistasis on the expression of yield, its component traits and grain Fe and Zn content and inadequacy of additive-dominance model to explain the variation in different generations. Dominance [h] gene effect was of higher magnitude as compared to additive [d] gene effect for both grain iron and grain zinc content.  Additive × additive, additive × dominance and dominance × dominance component was significant for both grain Fe and Zn content, whereas dominance × dominance component was predominant for both grain Fe and Zn content. Dominance [h] gene effect and dominance × dominance interaction acted in opposite directions, indicating duplicate type of gene action controlling the expression of both grain Fe and grain Zn content which could be a bottleneck to exploit heterosis. Heterosis breeding and recombination breeding with postponement of selection till later generations, could be effective in improving both grain Fe and grain Zn content in rice.

scholarly journals Evaluation of biofortified spring wheat genotypes for yield and micronutrients

2018 ◽  
Vol 10 (1) ◽  
pp. 210-215 ◽  
Author(s):  
Ravish Chatrath ◽  
Vikas Gupta ◽  
Om Parkash ◽  
Gyanendra Pratap Singh
Keyword(s):  
Significant Variation ◽  
Significant Positive Correlation ◽  
Principal Component ◽  
Zinc Content ◽  
Wheat Genotypes ◽  
Fe Content ◽  
Days To Heading ◽  
Zn Content ◽  
Days To Maturity ◽  
Iron And Zinc

Advanced wheat genotypes were tested for agronomic as well as grain iron and zinc content traits. The analysis of variance indicated variation for all traits except iron (Fe) and zinc (Zn). The grain Fe content ranged from 39-58 mg/kg whereas grain Zn ranged from 32-47 mg/kg among the tested lines. A significant positive correlation (0.45) was observed between grain Fe and Zn content. There was no association between yield and grain Fe and Zn content indicating that improvement in these micronutrients will not have any undesirable affect on yield. The data was further analysed for principal component analysis and genotype by trait association. The first five principal components viz., PC1 (0.3149), PC2 (0.2198), PC3 (0.1461), PC4 (0.10) and PC5 (0.0923) accounted for 0.87 of the total variation. The major traits contributing to the PC1 are days to heading, days to maturity, grain iron content and yield. The cluster analysis revealed significant variation among the tested germplasm thus providing opportunities for increasing the micronutrient content along with yield through hybridization with high micronutrient content lines.

scholarly journals Deciphering Genomic Regions for High Grain Iron and Zinc Content Using Association Mapping in Pearl Millet

2017 ◽  
Vol 8 ◽  
Author(s):  
N. Anuradha ◽  
C. Tara Satyavathi ◽  
C. Bharadwaj ◽  
T. Nepolean ◽  
S. Mukesh Sankar ◽  
...  
Keyword(s):  
Association Mapping ◽  
Pearl Millet ◽  
Zinc Content ◽  
Iron And Zinc ◽  
Genomic Regions

scholarly journals ‘Hyangcheola’, A New Fragrant Rice Variety with High Iron and Zinc Content

2018 ◽  
Vol 50 (4) ◽  
pp. 472-477
Author(s):  
Eung-Gi Jeong ◽  
Eok-Keun Ahn ◽  
Yong-Jae Won ◽  
Jeong-Heui Lee ◽  
Sang-Bok Lee ◽  
...  
Keyword(s):  
Rice Variety ◽  
Zinc Content ◽  
High Iron ◽  
Fragrant Rice ◽  
Iron And Zinc
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