The Terpene Synthase Gene Family of Carrot (Daucus carota L.): Identification of QTLs and Candidate Genes Associated with Terpenoid Volatile Compounds

The behavior of people who less consume vegetables and fruit can have a negative impact on health. The lack of appetite for vegetable consumption by the society made the researchers interested in making a combination of vegetables, namely carrots with local foods, namely Baroncong.This research aims to determine the acceptability of local baroncong snacks with the addition of carrots.This research is a pre-experimental study. The panelists from this study consisted of 30 Nutrition Department Students from Health Polytechnic Ministry of Health Makassar. Assessment based on taste aspects, color of aroma and texture of baroncong products with the addition of carrots 50%, 75%, 100% with the criteria of very like, like, dislike and very dislike.The results showed that the acceptability of baroncong with the addition of carrots in terms of taste was a concentration of 75%, which was very much as much as 46.7% and liked as much as 50%, the color aspect was 50% concentration consisting of 36.7% likes and likes as much as 53.3%, the aroma aspectis a concentration of 50% which consists of very much as much as 33.3% and likes as much as 63.3%, in terms of aspects of texture are concentrations of 50% and 75%, each of which consists of as much as 23.3% and likes as much as 63, 3%. Based on these data, Baroncong with the addition of carrots received is a concentration of 75%.

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ESTUDO DA CINÉTICA DE SECAGEM EM CENOURAS (DAUCUS CAROTA L.)

10.31692/2526-7701.iiicointerpdvagro.2018.00123 ◽

2018 ◽

Author(s):

Araújo Thaís Jaciane ◽

Santos Newton Carlos ◽

Barros Sâmela Leal ◽

Melo Mylena Olga Pessoa ◽

Nascimento Amanda Priscila Silva

Keyword(s):

Daucus Carota ◽

Daucus Carota L

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Anthocyanin accumulation and PAL activity in a suspension culture of Daucus carota L.

Planta ◽

10.1007/bf00384567 ◽

1980 ◽

Vol 149 (3) ◽

pp. 283-287 ◽

Cited By ~ 48

Author(s):

Wolfgang No� ◽

Christian Langebartels ◽

Hanns Ulrich Seitz

Keyword(s):

Suspension Culture ◽

Daucus Carota ◽

Anthocyanin Accumulation ◽

Daucus Carota L ◽

Pal Activity

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Total β-carotene of β-carotene carrot powder (Daucus Carota L.) encapsulation result

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/443/1/012063 ◽

2020 ◽

Vol 443 ◽

pp. 012063

Author(s):

M Rifqi ◽

I S Setiasih ◽

Y Cahayana

Keyword(s):

Daucus Carota ◽

Daucus Carota L ◽

Β Carotene

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Daucus carota L. Mediated Bioreduction of Prochiral Ketones

ChemInform ◽

10.1002/chin.200642224 ◽

2006 ◽

Vol 37 (42) ◽

Author(s):

Nicolas Blanchard ◽

Pierre van de Weghe

Keyword(s):

Daucus Carota ◽

Daucus Carota L ◽

Prochiral Ketones

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Effect of tillage, biochar, poultry manure and NPK 15-15-15 fertilizer, and their mixture on soil properties, growth and carrot (Daucus carota L.) yield under tropical conditions

Heliyon ◽

10.1016/j.heliyon.2021.e07391 ◽

2021 ◽

pp. e07391

Author(s):

Taiwo Michael Agbede

Keyword(s):

Soil Properties ◽

Daucus Carota ◽

Poultry Manure ◽

Daucus Carota L ◽

Tropical Conditions

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Genome-Wide Analysis of Terpene Synthase Gene Family in Mentha longifolia and Catalytic Activity Analysis of a Single Terpene Synthase

Genes ◽

10.3390/genes12040518 ◽

2021 ◽

Vol 12 (4) ◽

pp. 518

Author(s):

Zequn Chen ◽

Xiwu Qi ◽

Xu Yu ◽

Ying Zheng ◽

Zhiqi Liu ◽

...

Keyword(s):

Catalytic Activity ◽

Essential Oils ◽

Gene Family ◽

Genome Sequence ◽

Sequence Assembly ◽

Terpene Synthase ◽

Genome Sequence Assembly ◽

Mentha Longifolia ◽

Specific Expansion ◽

Species Specific

Terpenoids are a wide variety of natural products and terpene synthase (TPS) plays a key role in the biosynthesis of terpenoids. Mentha plants are rich in essential oils, whose main components are terpenoids, and their biosynthetic pathways have been basically elucidated. However, there is a lack of systematic identification and study of TPS in Mentha plants. In this work, we genome-widely identified and analyzed the TPS gene family in Mentha longifolia, a model plant for functional genomic research in the genus Mentha. A total of 63 TPS genes were identified in the M. longifolia genome sequence assembly, which could be divided into six subfamilies. The TPS-b subfamily had the largest number of genes, which might be related to the abundant monoterpenoids in Mentha plants. The TPS-e subfamily had 18 members and showed a significant species-specific expansion compared with other sequenced Lamiaceae plant species. The 63 TPS genes could be mapped to nine scaffolds of the M. longifolia genome sequence assembly and the distribution of these genes is uneven. Tandem duplicates and fragment duplicates contributed greatly to the increase in the number of TPS genes in M. longifolia. The conserved motifs (RR(X)8W, NSE/DTE, RXR, and DDXXD) were analyzed in M. longifolia TPSs, and significant differentiation was found between different subfamilies. Adaptive evolution analysis showed that M. longifolia TPSs were subjected to purifying selection after the species-specific expansion, and some amino acid residues under positive selection were identified. Furthermore, we also cloned and analyzed the catalytic activity of a single terpene synthase, MlongTPS29, which belongs to the TPS-b subfamily. MlongTPS29 could encode a limonene synthase and catalyze the biosynthesis of limonene, an important precursor of essential oils from the genus Mentha. This study provides useful information for the biosynthesis of terpenoids in the genus Mentha.

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