Resveratrol Alleviates the KCl Salinity Stress of Malus hupehensis Rhed

Applying large amounts of potash fertilizer in apple orchards for high apple quality and yield aggravates KCl stress. As a phytoalexin, resveratrol (Res) participates in plant resistance to biotic stress. However, its role in relation to KCl stress has never been reported. Herein we investigated the role of Res in KCl stress response of Malus hupehensis Rehd., a widely used apple rootstock in China which is sensitive to KCl stress. KCl-stressed apple seedlings showed significant wilting phenotype and decline in photosynthetic rate, and the application of 100 μmol Res alleviated KCl stress and maintained photosynthetic capacity. Exogenous Res can strengthen the activities of peroxidase and catalase, thus eliminating reactive oxygen species production induced by KCl stress. Moreover, exogenous Res can decrease the electrolyte leakage by accumulating proline for osmotic balance under KCl stress. Furthermore, exogenous Res application can affect K+/Na+ homeostasis in cytoplasm by enhancing K+ efflux outside the cells, inhibiting Na+ efflux and K+ absorption, and compartmentalizing K+ into vacuoles through regulating the expression of K+ and Na+ transporter genes. These findings provide a theoretical basis for the application of exogenous Res to relieve the KCl stress of apples.

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Differential sensitivity of human monocytes and macrophages to ANP: a role of intracellular pH on reactive oxygen species production through the phospholipase involvement

Journal of Leukocyte Biology ◽

10.1189/jlb.0702377 ◽

2003 ◽

Vol 73 (4) ◽

pp. 502-510 ◽

Cited By ~ 26

Author(s):

P. M. Baldini ◽

P. De Vito ◽

A. Martino ◽

M. Fraziano ◽

C. Grimaldi ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Intracellular Ph ◽

Reactive Oxygen Species Production ◽

Differential Sensitivity ◽

Oxygen Species ◽

Human Monocytes ◽

Monocytes And Macrophages ◽

Species Production ◽

Human Monocytes And Macrophages

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PINK1 deficiency impairs osteoblast differentiation through aberrant mitochondrial homeostasis

Stem Cell Research & Therapy ◽

10.1186/s13287-021-02656-4 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

So-Young Lee ◽

Hyun-Ju An ◽

Jin Man Kim ◽

Min-Ji Sung ◽

Do Kyung Kim ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Quality Control ◽

Reactive Oxygen Species Production ◽

Reactive Oxygen ◽

Oxygen Species ◽

Mitochondrial Quality Control ◽

Mitochondrial Homeostasis ◽

Ovariectomized Mice ◽

Species Production

Abstract Background PTEN-induced kinase 1 (PINK1) is a serine/threonine-protein kinase in mitochondria that is critical for mitochondrial quality control. PINK1 triggers mitophagy, a selective autophagy of mitochondria, and is involved in mitochondrial regeneration. Although increments of mitochondrial biogenesis and activity are known to be crucial during differentiation, data regarding the specific role of PINK1 in osteogenic maturation and bone remodeling are limited. Methods We adopted an ovariectomy model in female wildtype and Pink1−/− mice. Ovariectomized mice were analyzed using micro-CT, H&E staining, Masson’s trichrome staining. RT-PCR, western blot, immunofluorescence, alkaline phosphatase, and alizarin red staining were performed to assess the expression of PINK1 and osteogenic markers in silencing of PINK1 MC3T3-E1 cells. Clinical relevance of PINK1 expression levels was determined via qRT-PCR analysis in normal and osteoporosis patients. Results A significant decrease in bone mass and collagen deposition was observed in the femurs of Pink1−/− mice after ovariectomy. Ex vivo, differentiation of osteoblasts was inhibited upon Pink1 downregulation, accompanied by impaired mitochondrial homeostasis, increased mitochondrial reactive oxygen species production, and defects in mitochondrial calcium handling. Furthermore, PINK1 expression was reduced in bones from patients with osteoporosis, which supports the practical role of PINK1 in human bone disease. Conclusions In this study, we demonstrated that activation of PINK1 is a requisite in osteoblasts during differentiation, which is related to mitochondrial quality control and low reactive oxygen species production. Enhancing PINK1 activity might be a possible treatment target in bone diseases as it can promote a healthy pool of functional mitochondria in osteoblasts.

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Resveratrol Alleviates the KCl Salinity Stress of Malus hupenensis Rhed. Seedlings by Regulating K+/Na+ Homeostasis, Osmotic Adjustment, and Reactive Oxygen Species Scavenging

10.21203/rs.3.rs-148384/v1 ◽

2021 ◽

Author(s):

Tingting Li ◽

Yuqi Li ◽

Zhijuan Sun ◽

Xiangli Xi ◽

Guangli Sha ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Osmotic Stress ◽

Salinity Stress ◽

Osmotic Adjustment ◽

Reactive Oxygen ◽

Oxygen Species ◽

Apple Rootstock ◽

Physiological Mechanisms ◽

Species Production ◽

Apple Quality

Abstract Background: Applying large amounts of potash fertilizer in apple orchards for high apple quality and yield aggravates KCl stress. As a phytoalexin, resveratrol (Res) participates in plant resistance to biotic stress. However, its role in relation to KCl stress have never been reported. Results: Malus hupenensis is a widely used apple rootstock in China and is sensitive to KCl stress. In this study, 100 µmol Res was exogenously applied to alleviate KCl stress in M. hupenensis seedlings. The seedlings treated with Res had higher chlorophyll content and photosynthetic index than those without Res treatment. Moreover, the molecular and physiological mechanisms of Res in ion toxicity, osmotic stress, and oxidative damage induced by KCl stress were also investigated. First, exogenous Res affects K+/Na+ homeostasis in cytoplasm by enhancing K+ efflux outside the cells, inhibiting Na+ efflux and K+ absorption, and compartmentalizing K+ into vacuoles. Second, this compound could respond to osmotic stress by regulating the accumulation of proline. Lastly, this polyphenol functions as an antioxidant that strengthens the activities of POD and CAT thus eliminates the reactive oxygen species production induced by KCl stress.Conclusions: Taken together, these results reveal that resveratrol alleviates the KCl salinity stress of M. hupenensis seedlings by regulating K+/Na+ homeostasis, osmotic adjustment, and reactive oxygen species scavenging.

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