scholarly journals Wheat Apoplast-Localized Lipid Transfer Protein TaLTP3 Enhances Defense Responses Against Puccinia triticina

2021 ◽  
Vol 12 ◽  
Author(s):  
Jiaojie Zhao ◽  
Weishuai Bi ◽  
Shuqing Zhao ◽  
Jun Su ◽  
Mengyu Li ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Lipid Transfer Protein ◽  
Puccinia Triticina ◽  
Defense Responses ◽  
Transgenic Wheat ◽  
Lipid Transfer ◽  
Transfer Protein ◽  
Pathogenesis Related ◽  
Rust Pathogen ◽  
Wheat Lines

Plant apoplast serves as the frontier battlefield of plant defense in response to different types of pathogens. Many pathogenesis-related (PR) proteins are accumulated in apoplastic space during the onset of plant–pathogen interaction, where they act to suppress pathogen infection. In this study, we found the expression of Triticum aestivum lipid transfer protein 3 (TaLTP3) gene was unregulated during incompatible interaction mediated by leaf rust resistance genes Lr39/41 at the early infection stage. Stable transgenic wheat lines overexpressing TaLTP3 exhibited enhanced resistance to leaf rust pathogen Puccinia triticina. Transcriptome analysis revealed that overexpression of TaLTP3 specifically activated the transcription of pathogenesis-related protein 1a (TaPR1a) and multiple plant hormone pathways, including salicylic acid (SA), jasmonic acid (JA), and auxin, in response to the infection of the model bacterial pathogen Pseudomonas syringae pv. tomato DC3000. Further investigation indicated that TaLTP3 physically associated with wheat TaPR1a protein in the apoplast. Transgenic wheat lines overexpressing TaLTP3 and TaPR1a showed higher accumulations of reactive oxygen species (ROS) during plant defense responses. All these findings suggested that TaLTP3 is involved in wheat resistance against leaf rust pathogen infection and forming a TaLTP3-TaPR1a complex in apoplast against this pathogen, which provides new insights into the functional roles of PR proteins.

A lipid transfer protein has antifungal and antioxidant activity and suppresses Fusarium head blight disease and DON accumulation in transgenic wheat

2020 ◽  
Author(s):  
John E McLaughlin ◽  
Noura Al Darwish ◽  
Jeffrey Garcia-Sanchez ◽  
Neerja Tyagi ◽  
Harold N Trick ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Fusarium Head Blight ◽  
Lipid Transfer Protein ◽  
Field Testing ◽  
Transgenic Wheat ◽  
Lesion Size ◽  
Lipid Transfer ◽  
Head Blight ◽  
Transfer Protein ◽  
Field Suppression

Trichothecene mycotoxins such as deoxynivalenol (DON), are virulence factors of Fusarium graminearum, which causes Fusarium head blight (FHB), one of the most important diseases of small grain cereals. We previously identified a non-specific lipid transfer protein (nsLTP) gene, AtLTP4.4, which was overexpressed in an activation tagged Arabidopsis line resistant to trichothecin (Tcin), a type B trichothecene in the same class as DON. Here we show that overexpression of AtLTP4.4 in transgenic wheat significantly reduced F. graminearum growth in Bobwhite and RB07 lines in the greenhouse and reduced fungal lesion size in detached leaf assays. Hydrogen peroxide accumulation was attenuated upon exposure of transgenic wheat plants to DON, indicating that AtLTP4.4 may confer resistance by inhibiting oxidative stress. Field testing indicated that disease severity was significantly reduced in two transgenic Bobwhite lines expressing AtLTP4.4. DON accumulation was significantly reduced in four different transgenic Bobwhite lines expressing AtLTP4.4 or a wheat nsLTP, TaLTP3, which was previously shown to have antioxidant activity. Recombinant AtLTP4.4 purified from Pichia pastoris exhibited potent antifungal activity against F. graminearum. These results demonstrate that overexpression of AtLTP4.4 in transgenic wheat suppresses DON accumulation in the field. Suppression of DON induced reactive oxygen species by AtLTP4.4 might be the mechanism by which fungal spread and mycotoxin accumulation are inhibited in the transgenic wheat plants.

scholarly journals Rust effector PNPi interacting with wheat TaPR1a attenuates plant defense response

2020 ◽  
Vol 2 (1) ◽  
Author(s):  
Weishuai Bi ◽  
Shuqing Zhao ◽  
Jiaojie Zhao ◽  
Jun Su ◽  
Xiumei Yu ◽  
...  
Keyword(s):  
Plant Defense ◽  
Pseudomonas Syringae ◽  
Systemic Acquired Resistance ◽  
Puccinia Striiformis ◽  
Acquired Resistance ◽  
Puccinia Triticina ◽  
Defense Responses ◽  
Pathogenesis Related ◽  
Exogenous Expression ◽  
Wheat Lines

Abstract NPR1 is a key regulator of systemic acquired resistance (SAR) in plant species. In our previous study, we identified a conserved fungal effector PNPi from Puccinia striiformis f. sp. tritici (Pst) that can suppress acquired resistance in local leaf by directly targeting the wheat NPR1 protein. In this investigation, we identified and validated a novel protein interaction between PNPi and wheat pathogenesis-related TaPR1a in the apoplastic space. TaPR1a-overexpressing wheat lines exhibited enhanced resistance to both Pst and Puccinia triticina (Pt). We further determined that exogenous expression of PNPi RNA in transgenic wheat lines reduced the degree of acquired resistance to Magnaporthe oryzae isolate P131 in the region adjacent to Pseudomonas syringae pv. tomato DC3000 infection area. Additionally, when PNPi was overexpressed, the expression levels of two plant defense responsive genes were suppressed upon P. syringae DC3000 infection in the local infiltration region. These findings established the mechanism of a single rust effector that can suppress multiple defense responses in wheat plants by targeting different components.

scholarly journals Expression of apoplast-targeted plant defensin MtDef4.2 confers resistance to leaf rust pathogen Puccinia triticina but does not affect mycorrhizal symbiosis in transgenic wheat

2016 ◽  
Vol 26 (1) ◽  
pp. 37-49 ◽  
Author(s):  
Jagdeep Kaur ◽  
John Fellers ◽  
Alok Adholeya ◽  
Siva L. S. Velivelli ◽  
Kaoutar El-Mounadi ◽  
...  
Keyword(s):  
Leaf Rust ◽  
Puccinia Triticina ◽  
Transgenic Wheat ◽  
Mycorrhizal Symbiosis ◽  
Plant Defensin ◽  
Rust Pathogen

scholarly journals Case series of seven cases of urticaria, angioedema, and anaphylaxis (LTP syndrome) due to foods (nuts, lentils, and citrus foods) related to tree pollen (Prosopis juliflora and Holoptelea integrifolia) sensitization

2021 ◽  
pp. 319-323
Author(s):  
P C Kathuria ◽  
Manisha Rai
Keyword(s):  
Lipid Transfer Protein ◽  
Case Series ◽  
Prosopis Juliflora ◽  
Cross Reactivity ◽  
Lipid Transfer ◽  
Allergenic Protein ◽  
Reactive Protein ◽  
Transfer Protein ◽  
Pathogenesis Related ◽  
Primary Sensitization

Lipid transfer protein syndrome (LTPS) results from a primary sensitization to LTP belonging to the PR-14 family of pathogenesis-related PR-proteins which are plant food allergens, stable against heat and digestive enzymes, and can induce systemic reactions of urticaria, angioedema, and anaphylaxis. Here, we report a case series of seven patients with nsLTP syndrome. Our six cases of LTPS were significantly sensitized to Prosopis juliflora and Holoptelea integrifolia and one case was sensitized to H. integrifolia only. In our seven cases, we hypothesized that symptoms of hypersensitivity reaction to foods were due to cross-reactivity by IgE reactive protein of ns-LTP as a major allergenic protein in foods.

Sign in / Sign up

Export Citation Format

Share Document