scholarly journals Calcium-Dependent Protein Kinase GhCDPK28 Was Dentified and Involved in Verticillium Wilt Resistance in Cotton

2021 ◽  
Vol 12 ◽  
Author(s):  
Yajie Wu ◽  
Lei Zhang ◽  
Jinglong Zhou ◽  
Xiaojian Zhang ◽  
Zili Feng ◽  
...  
Keyword(s):  
Verticillium Wilt ◽  
Defense Responses ◽  
Economic Losses ◽  
Cis Acting ◽  
Calcium Dependent ◽  
Resistant Varieties ◽  
Gossypium Raimondii ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinases ◽  
Calcium Dependent Protein Kinase

Verticillium dahliae is a soil-borne fungus that causes vascular wilt through the roots of plants. Verticillium wilt caused by V. dahliae is one of the main diseases in cotton producing areas of the world, resulting in huge economic losses. Breeding resistant varieties is the most economical and effective method to control Verticillium wilt. Calcium-dependent protein kinases (CDPKs) play a pivotal role in plant innate immunity, including regulation of oxidative burst, gene expression as well as hormone signal transduction. However, the function of cotton CDPKs in response to V. dahliae stress remains unexplored. In this study, 96, 44 and 57 CDPKs were identified from Gossypium hirsutum, Gossypium raimondii and Gossypium arboretum, respectively. Phylogenetic analysis showed that these CDPKs could be divided into four branches. All GhCDPKs of the same clade are generally similar in gene structure and conserved domain arrangement. Cis-acting elements related to hormones, stress response, cell cycle and development were predicted in the promoter region. The expression of GhCDPKs could be regulated by various stresses. Gh_D11G188500.1 and Gh_A11G186100.1 was up-regulated under Vd0738 and Vd991 stress. Further phosphoproteomics analysis showed that Gh_A11G186100.1 (named as GhCDPK28-6) was phosphorylated under the stress of V. dahliae. Knockdown of GhCDPK28-6 expression, the content of reactive oxygen species was increased, a series of defense responses were enhanced, and the sensitivity of cotton to V. dahliae was reduced. Moreover, overexpression of GhCDPK28-6 in Arabidopsis thaliana weakened the resistance of plants to this pathogen. Subcellular localization revealed that GhCDPK28-6 was localized in the cell membrane. We also found that GhPBL9 and GhRPL12C may interact with GhCDPK28-6. These results indicate that GhCDPK28-6 is a potential molecular target for improving resistance to Verticillium wilt in cotton. This lays a foundation for breeding disease-resistant varieties.

scholarly journals Structure and functions of plant calcium-dependent protein kinases.

2007 ◽  
Vol 54 (2) ◽  
pp. 219-233 ◽  
Author(s):  
Maria Klimecka ◽  
Grazyna Muszyńska
Keyword(s):  
Protein Kinases ◽  
Calcium Ions ◽  
Second Messengers ◽  
Calcium Content ◽  
Defense Responses ◽  
Calcium Dependent ◽  
Cytoskeleton Organization ◽  
Calcium Sensors ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinases

Calcium ions as second messengers play an essential role in many important cellular processes. In plants, transient changes in calcium content in the cytosol (calcium signatures) have been observed during growth, development and under stress conditions. Such diverse functions require many different calcium sensors. One of the largest and most differentiated group of calcium sensors are protein kinases, among them calcium-dependent protein kinases (CDPKs) which were identified only in plants and protists. CDPKs have a regulatory domain which is able to bind calcium ions. For regulation of CDPKs activities not only calcium ions but also specific phospholipids and autophosphorylation are responsible. CDPKs have many different substrates, which reflects the diversity of their functions. Potential protein substrates of CDPK are involved in carbon and nitrogen metabolism, phospholipid synthesis, defense responses, ion and water transport, cytoskeleton organization, transcription and hormone responses. Presently, participation of CDPKs in stress signal transduction pathways (e.g., cold, drought, high salinity, wounding) is intensively studied in many laboratories. An intriguing, but still not fully clarified problem is the cross-talk via CDPKs among different signaling pathways that enables signal integration at different levels and ensure appropriate downstream responses.

scholarly journals The calcium-dependent protein kinase 1 from Toxoplasma gondii as target for structure-based drug design

Parasitology ◽  
2017 ◽  
Vol 145 (2) ◽  
pp. 210-218 ◽  
Author(s):  
EMILY M. CARDEW ◽  
CHRISTOPHE L. M. J. VERLINDE ◽  
EHMKE POHL
Keyword(s):  
Toxoplasma Gondii ◽  
Complex Life Cycle ◽  
Dependent Protein Kinase ◽  
Future Directions ◽  
Structure Based Drug Design ◽  
Calcium Dependent ◽  
Causative Agents ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinases ◽  
Calcium Dependent Protein Kinase

SummaryThe apicomplexan protozoan parasites include the causative agents of animal and human diseases ranging from malaria (Plasmodium spp.) to toxoplasmosis (Toxoplasma gondii). The complex life cycle of T. gondii is regulated by a unique family of calcium-dependent protein kinases (CDPKs) that have become the target of intensive efforts to develop new therapeutics. In this review, we will summarize structure-based strategies, recent successes and future directions in the pursuit of specific and selective inhibitors of T. gondii CDPK1.

scholarly journals Multisite phosphorylation of 14-3-3 proteins by calcium-dependent protein kinases

2014 ◽  
Vol 459 (1) ◽  
pp. 15-25 ◽  
Author(s):  
Kirby N. Swatek ◽  
Rashaun S. Wilson ◽  
Nagib Ahsan ◽  
Rebecca L. Tritz ◽  
Jay J. Thelen
Keyword(s):  
Protein Kinase ◽  
Protein Kinases ◽  
Functional Role ◽  
Dependent Protein Kinase ◽  
Calcium Dependent ◽  
Multisite Phosphorylation ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinases ◽  
Calcium Dependent Protein Kinase

This paper describes calcium-dependent protein kinase (CPK) preference for 14-3-3 substrates, novel 14-3-3 phosphorylation and CPK autophosphorylation sites, and a functional role for 14-3-3 phosphorylation.

Calcium-Dependent Protein Kinases (CDPK) in Abiotic Stress Tolerance

2018 ◽  
Vol 34 (2) ◽  
pp. 259-265 ◽  
Author(s):  
Hemant B Kardile ◽  
◽  
Vikrant ◽  
Nirmal Kant Sharma ◽  
Ankita Sharma ◽  
...  
Keyword(s):  
Abiotic Stress ◽  
Stress Tolerance ◽  
Protein Kinases ◽  
Abiotic Stress Tolerance ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinases

scholarly journals A Novel Calcium-Dependent Protein Kinase 1 Inhibitor Potently Prevents Toxoplasma gondii Transmission to Foetuses in Mouse

Molecules ◽  
2021 ◽  
Vol 26 (14) ◽  
pp. 4203
Author(s):  
Héloïse Débare ◽  
Nathalie Moiré ◽  
Firmin Baron ◽  
Louis Lantier ◽  
Bruno Héraut ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Toxoplasma Gondii ◽  
Intraperitoneal Administration ◽  
Congenital Toxoplasmosis ◽  
Parasite Burden ◽  
Oral Route ◽  
Dependent Protein Kinase ◽  
Calcium Dependent ◽  
Dependent Protein ◽  
Calcium Dependent Protein Kinase

Treatments currently used to prevent congenital toxoplasmosis are non-specific of Toxoplasma gondii and have grievous side effects. To develop a more specific and less toxic drug, we have designed SP230, an imidazo[1,2-b]pyridazine salt targeting the Toxoplasma gondii calcium-dependent protein kinase 1 (TgCDPK1) and active against acute toxoplasmosis in mice. Efficiency of SP230 to inhibit foetal transmission of the parasite was evaluated in a mouse model of congenital toxoplasmosis. Swiss mice were infected at mid-pregnancy with tachyzoites or cysts of the ME49 strain of T. gondii by intraperitoneal and oral route, respectively, and treated with SP230 at 50 mg/kg for 5 days by the same routes. Parasite burden in organs of dams and in foetuses was measured by quantitative PCR. Intraperitoneal administration of SP230 drastically reduced the number of parasites (more than 97% of reduction) in the brain and lungs of dams, and led to a reduction of 66% of parasite burden in foetuses. Oral administration of SP230 was particularly efficient with 97% of reduction of parasite burdens in foetuses. SP230 did not impact number and weight of offspring in our conditions. This inhibitor of TgCDPK1 is a promising candidate for the development of alternative therapeutics to treat infected pregnant women.

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