scholarly journals Distinct Root Microbial Communities in Nature Farming Rice Harbor Bacterial Strains With Plant Growth-Promoting Traits

2021 ◽  
Vol 4 ◽  
Author(s):  
Grace Flavyeliz Sinong ◽  
Michiko Yasuda ◽  
Yoshiyuki Nara ◽  
Chol Gyu Lee ◽  
Khondoker Mohammad Golam Dastogeer ◽  
...  
Keyword(s):  
Plant Growth ◽  
Farming Systems ◽  
Farming System ◽  
Bacterial Strains ◽  
Chemical Fertilizers ◽  
Distinct Root ◽  
Rice Growth ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Root Microbiome

A nature farming system is an ecological farming practice that entails cultivating crops without using chemical fertilizers and pesticides. To understand the diversity and functions of root microbiomes associated with nature farming systems, we compared the root microbial community of rice under nature farming conditions with those under conventional farming conditions. High-throughput amplicon analysis demonstrated a higher abundance and greater diversity of the root microbiome under unfertilized nature farming conditions than under conventional conditions. The application of chemical fertilizers reduced the microbial diversity and abundance of some beneficial taxa important for plant growth and health. Subsequently, we isolated and identified 46 endo- and epiphytic bacteria from rice roots grown under nature farming conditions and examined their plant growth-promoting activity. Six potential isolates were selected for plant growth assessment in insoluble P- and K-containing media. Most of the isolates promoted rice growth, and Pseudomonas koreensis AEPR1 was able to enhance rice growth significantly in both insoluble P- and K-containing media. Our data indicated that nature farming systems create a distinct root microbiome that is comparatively more diverse and supports plant growth under low-input cultivation practices than under conventional practices. The potential isolates could be exploited as sources with potential applications in sustainable agriculture.

scholarly journals The use of bacterial indol-3-acetic acid (IAA) for reduce of chemical fertilizers doses

2017 ◽  
Vol 71 (3) ◽  
pp. 195-200 ◽  
Author(s):  
Snezana Djordjevic ◽  
Dragana Stanojevic ◽  
Milka Vidovic ◽  
Violeta Mandic ◽  
Ivana Trajkovic
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Pcr Amplification ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Chemical Fertilizers ◽  
Positive Effects ◽  
Plant Growth Promoting ◽  
Growth Promoting

The standard technology of seed processing uses mainly chemical products. Recent researches showed that toxic materials from chemical fertilizers can be harmful to humans, animals and the environment. Currently the attention of researches is shifting away from chemical fertlizers and toward alternative that consumers perceive to be natural, Plant Growth Promoting bacteria (PGP). PGP bacteria could be a way to reduce chemical fertilizer doses. This was the reason to test the ability of Bacillus megaterium, Azotobacter chroococcum to produce hormone auxin (IAA). Bacterial strains were identified by PCR amplification and sequencing of the 16S rRNA gene. Indole-3-acetic acid (IAA) was detected and quantified by MRM experiment. This study conducted that maize seed inoculation with IAA from species mentioned above showed positive effects. They had statistically significantly higher root and steam height compared to control seedlings. Bacterial strains tested in this study may be recommended as PGP (Plant Growth Promoting) bacteria, due to their positive effects and eventually can be used to reduce chemical fertilizers doses.

scholarly journals Study of the Modulating Interactions of Multitrait Rhizobacteria Using Zea Mays L. as the Host Plant

2020 ◽  
Vol 10 (2) ◽  
pp. 44-53
Author(s):  
Sana Shakeel ◽  
Ifrah Javaid ◽  
Ambreen Ahmed
Keyword(s):  
Plant Growth ◽  
Crop Production ◽  
Bacillus Pumilus ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Chemical Fertilizers ◽  
Streptomyces Lydicus ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Plant Growth Promoting Traits

Background: Rhizosphere is a soil region closest to roots of the plants inhabiting different types of microorganisms including rhizobacteria. Chemical fertilizers which are conventionally used for increasing crop production are dangerous in terms of minimizing the nutritional value of crops and may also be hazardous for biological agents. Therefore, the use of Plant Growth Promoting Rhizobacteria (PGPR) are favorable for improved crop production over chemical fertilizers. Objectives: The current study highlights the growth promoting traits of bacterial isolates through isolation of rhizospheric bacterial strains from different plants. Methodology: In this study, ten rhizospheric bacterial isolates were used, which were morpho-physiologically characterized and then tested for plant growth-promoting traits i.e., HCN production, ammonification and auxin production. Most of the bacterial strains gave positive results for these plant growth-promoting traits. To study the beneficial effects of these bacteria on plants, plant-microbial interaction assay was conducted using Zea mays. Results: Results revealed that these bacteria enhanced the growth as compared to control plants. Bacterial isolates Streptomyces lydicus (Cn6), Staphylococcus aureus (Cn7) and Bacillus pumilus (PP3) showed strong ammonia producing effects. The isolates Bacillus subtilis (Cn2), PP2 and PP5 exhibited strong potential of HCN production whereas only Streptomyces lydicus (Cn6) and Bacillus pumilus (PP3) were observed to be auxin producers. A maximum increase in fresh weight of the plants was observed in treatment with PP2 showing 94.36% increase over controls. Cn1 showed an increase (26.12%) in shoot length while Cn5 revealed a prominent increase (64.95%) in root length compared to the control plant. The isolates Cn5 and Cn4 showed improvement in the total chlorophyll content of the treated plants with a percentage increase of 100% and 99.82%, respectively compared to the control. Conclusion: In conclusion, these PGPR may be further used in agriculture research for growth improvement.

scholarly journals Response of Upland Rice (Oryza sativa L.) Inoculated with Non-Native Plant Growth-Promoting Bacteria

Agronomy ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 903
Author(s):  
Michel Ouyabe ◽  
Kenji Irie ◽  
Naoto Tanaka ◽  
Hidehiko Kikuno ◽  
Babil Pachakkil ◽  
...  
Keyword(s):  
Plant Growth ◽  
Upland Rice ◽  
Growth Parameters ◽  
Growth Chamber ◽  
Plant Growth Promoting Bacteria ◽  
Native Plant ◽  
Bacterial Strains ◽  
Rice Growth ◽  
Plant Growth Promoting ◽  
Growth Promoting

A deep-rooting upland rice variety (Kinandang Patong) was evaluated for its growth response to bio-fertilization at early stages. Five non-native plant growth-promoting bacteria previously isolated from yams (Dioscorea spp.) were inoculated to upland rice under growth chamber and greenhouse conditions. Effects of the inoculation varied depending on bacterial strains and growing conditions. Growth of 14-day rice seedlings was improved by all tested bacterial strains. Under growth chamber, the strain S-333 increased plant length, shoot dry weight and nitrogen content as compared to the control, but total dry weight, nitrogen uptake, leaf chlorophyll content and number of tillers were higher with N fertilizer application. Under greenhouse conditions, most rice growth parameters were improved by inoculation with the strain S-7. The correlations between the bacterial plant-growth-promoting traits and rice growth parameters under growth chamber conditions were all negative for phosphate solubilization indexes. Our results suggest that bacterial inoculation can replace half (S-343 and S-611) of or the full (S-7) rate of chemical N fertilizer required, depending on bacterial strains and growing environments, although δ15N value in control plants was lower than in inoculated plants under growth chamber conditions, suggesting that the bacteria improve rice growth through mechanisms other than biological nitrogen fixation.

scholarly journals Role of Two Plant Growth-Promoting Bacteria in Remediating Cadmium-Contaminated Soil Combined with Miscanthus floridulus (Lab.)

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 912
Author(s):  
Shuming Liu ◽  
Hongmei Liu ◽  
Rui Chen ◽  
Yong Ma ◽  
Bo Yang ◽  
...  
Keyword(s):  
Plant Growth ◽  
Contaminated Soils ◽  
Translocation Factor ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Pgp Traits ◽  
Cd Tolerance ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Cd Translocation

Miscanthus spp. are energy plants and excellent candidates for phytoremediation approaches of metal(loid)s-contaminated soils, especially when combined with plant growth-promoting bacteria. Forty-one bacterial strains were isolated from the rhizosphere soils and roots tissue of five dominant plants (Artemisia argyi Levl., Gladiolus gandavensis Vaniot Houtt, Boehmeria nivea L., Veronica didyma Tenore, and Miscanthus floridulus Lab.) colonizing a cadmium (Cd)-contaminated mining area (Huayuan, Hunan, China). We subsequently tested their plant growth-promoting (PGP) traits (e.g., production of indole-3-acetic acid, siderophore, and 1-aminocyclopropane-1-carboxylate deaminase) and Cd tolerance. Among bacteria, two strains, Klebsiella michiganensis TS8 and Lelliottia jeotgali MR2, presented higher Cd tolerance and showed the best results regarding in vitro growth-promoting traits. In the subsequent pot experiments using soil spiked with 10 mg Cd·kg−1, we investigated the effects of TS8 and MR2 strains on soil Cd phytoremediation when combined with M. floridulus (Lab.). After sixty days of planting M. floridulus (Lab.), we found that TS8 increased plant height by 39.9%, dry weight of leaves by 99.1%, and the total Cd in the rhizosphere soil was reduced by 49.2%. Although MR2 had no significant effects on the efficiency of phytoremediation, it significantly enhanced the Cd translocation from the root to the aboveground tissues (translocation factor > 1). The combination of K. michiganensis TS8 and M. floridulus (Lab.) may be an effective method to remediate Cd-contaminated soils, while the inoculation of L. jeotgali MR2 may be used to enhance the phytoextraction potential of M. floridulus.

Interactive effect of biochar and plant growth-promoting bacterial endophytes on ameliorating salinity stress in maize

2015 ◽  
Vol 42 (8) ◽  
pp. 770 ◽  
Author(s):  
Saqib Saleem Akhtar ◽  
Mathias Neumann Andersen ◽  
Muhammad Naveed ◽  
Zahir Ahmad Zahir ◽  
Fulai Liu
Keyword(s):  
Plant Growth ◽  
Salinity Stress ◽  
Crop Production ◽  
Interactive Effect ◽  
Nutrient Balance ◽  
Bacterial Strains ◽  
Negative Effects ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Salt Affected Soils

The objective of this work was to study the interactive effect of biochar and plant growth-promoting endophytic bacteria containing 1-aminocyclopropane-1-carboxylate deaminase and exopolysaccharide activity on mitigating salinity stress in maize (Zea mays L.). The plants were grown in a greenhouse under controlled conditions, and were subjected to separate or combined treatments of biochar (0% and 5%, w/w) and two endophytic bacterial strains (Burkholderia phytofirmans (PsJN) and Enterobacter sp. (FD17)) and salinity stress. The results indicated that salinity significantly decreased the growth of maize, whereas both biochar and inoculation mitigated the negative effects of salinity on maize performance either by decreasing the xylem Na+ concentration ([Na+]xylem) uptake or by maintaining nutrient balance within the plant, especially when the two treatments were applied in combination. Moreover, in biochar-amended saline soil, strain FD17 performed significantly better than did PsJN in reducing [Na+]xylem. Our results suggested that inoculation of plants with endophytic baterial strains along with biochar amendment could be an effective approach for sustaining crop production in salt-affected soils.

scholarly journals The Effects of Plant Growth-Promoting Bacteria with Biostimulant Features on the Growth of a Local Onion Cultivar and a Commercial Zucchini Variety

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 888
Author(s):  
Giorgia Novello ◽  
Patrizia Cesaro ◽  
Elisa Bona ◽  
Nadia Massa ◽  
Fabio Gosetti ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Global Market ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Mineral Concentration ◽  
Positive Effects ◽  
Plant Growth Promoting ◽  
Onion Cultivar ◽  
Growth Promoting

The reduction of chemical inputs due to fertilizer and pesticide applications is a target shared both by farmers and consumers in order to minimize the side effects for human and environmental health. Among the possible strategies, the use of biostimulants has become increasingly important as demonstrated by the fast growth of their global market and by the increased rate of registration of new products. In this work, we assessed the effects of five bacterial strains (Pseudomonas fluorescens Pf4, P. putida S1Pf1, P. protegens Pf7, P. migulae 8R6, and Pseudomonas sp. 5Vm1K), which were chosen according to their previously reported plant growth promotion traits and their positive effects on fruit/seed nutrient contents, on a local onion cultivar and on zucchini. The possible variations induced by the inoculation with the bacterial strains on the onion nutritional components were also evaluated. Inoculation resulted in significant growth stimulation and improvement of the mineral concentration of the onion bulb, induced particularly by 5Vm1K and S1Pf1, and in different effects on the flowering of the zucchini plants according to the bacterial strain. The present study provides new information regarding the activity of the five plant growth-promoting bacteria (PGPB) strains on onion and zucchini, two plant species rarely considered by the scientific literature despite their economic relevance.

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