scholarly journals Effect of Dried Distillers Grains With Solubles and Red Osier Dogwood Extract on Fermentation Pattern and Microbial Profiles of a High-Grain Diet in an Artificial Rumen System

2021 ◽  
Vol 8 ◽  
Author(s):  
Walaa Mohamed Sayed Gomaa ◽  
Atef Mohamed Saleem ◽  
Tao Ran ◽  
Long Jin ◽  
Mohamed Samir ◽  
...  

The objective of this study was to evaluate the effect of dried distillers grains with solubles (DDGS) and red-osier dogwood (ROD) extract on in vitro fermentation characteristics, nutrient disappearance, and microbial profiles using the rumen simulation technique. The experiment was a completely randomized design with a 2 × 2 factorial arrangement of treatments and four replicates per treatment. A basal diet [10% barley silage, 87% dry-rolled barley grain, and 3% vitamin and mineral supplement, dry matter (DM) basis] and a DDGS diet (as per basal diet with 25% of wheat DDGS replacing an equal portion of barley grain) were supplemented with ROD extract at 0 and 1% (DM basis), respectively. The experimental period was 17 d, consisting 10 days of adaptation and 7 days of data and sample collection. The substitution of wheat DDGS for barley grain did not affect gas production; disappearances of DM, organic matter, and crude protein; total volatile fatty acid (VFA) production; and microbial protein production. However, replacing barley grain with wheat DDGS increased (P = 0.01) fermenter pH and molar proportion of branched-chain VFA, switched (P = 0.06) the fermentation pattern to higher acetate production due to increased (P = 0.01) disappearance of neutral detergent fiber (NDF), and decreased (P = 0.08) methane (CH4) production. In the basal barley diet, the ROD extract increased the acetate to propionate (A:P) ratio (P = 0.08) and reduced the disappearance of starch (P = 0.06) with no effect on any other variables. No effects of ROD in the DDGS diet were observed. The number of operational taxonomic unit (OTUs) and the Shannon diversity index of the microbial community had little variation among treatments. Taxonomic analysis revealed no effect of adding the ROD extract on the relative abundance of bacteria at the phylum level with either the basal diet or DDGS diet, while at the genus level, the microbial community was affected by the addition of both DDGS and the ROD extract. Prevotella and Fibrobacter were the most abundant genera in the basal diet; however, Treponema became the most abundant genus with the addition of the ROD extract. These results indicated that the substitution of wheat DDGS for barley grain may mitigate enteric CH4 emissions. The trend of reduced starch fermentability and increased NDF disappearance with the addition of ROD extract suggests a reduced risk of rumen acidosis and an improvement in the utilization of fiber for cattle-fed high-grain diet.

2010 ◽  
Vol 90 (2) ◽  
pp. 259-269 ◽  
Author(s):  
L J Walter ◽  
J L Aalhus ◽  
W M Robertson ◽  
T A McAllister ◽  
D J Gibb ◽  
...  

A study was conducted on crossbred steers (n = 275; 376 ± 24 kg) to evaluate performance and carcass quality of cattle fed wheat or corn dried distillers’ grains with solubles (DDGS). The control ration contained 86.6% rolled barley grain, 5.7% supplement and 7.7% barley silage (DM basis). The four treatments included replacement of barley grain at 20 or 40% of the diet (DM basis) with wheat or corn DDGS. Steers were slaughtered at a common end weight of 645 kg with 100 steers randomly (n = 20 per treatment) selected for determination of the retail yield of sub-primal boneless boxed beef (SPBBB). Data were analyzed as a completely randomized design using pen as the experimental unit. Feeding increasing levels of wheat DDGS led to a quadratic increase in dry matter intake (DMI) (P < 0.01), whereas increasing levels of corn DDGS led to a quadratic decrease in DMI (P = 0.01). Average daily gain was not influenced (P = 0.13) by feeding wheat or corn DDGS, but cattle fed corn DDGS exhibited a quadratic increase (P = 0.01) in gain:feed. As a result, a quadratic increase (P < 0.01) in calculated NEg of the diet was observed as corn DDGS levels increased. A linear decrease (P = 0.04) in days on feed (169, 166 and 154 d) was noted when increasing levels of wheat DDGS (0, 20 and 40%) were fed. Dressing percentage increased in a linear fashion with wheat DDGS (P < 0.01) inclusion level and in a quadratic fashion (P = 0.01) as corn DDGS inclusion level increased although other carcass traits were not affected (P > 0.10) by treatment. The results indicate that replacement of barley grain with corn or wheat DDGS up to 40% of the diet (DM) can lead to superior performance (improved gain:feed or reduced days on feed, respectively) with no detrimental effect on quality grade or carcass SPBBB yield.Key words: Dried distillers’ grains with solubles, corn, wheat, feedlot performance, carcass quality, steers


2014 ◽  
Vol 77 (11) ◽  
pp. 1968-1975 ◽  
Author(s):  
ROBIN C. ANDERSON ◽  
ROGER B. HARVEY ◽  
TRYON A. WICKERSHAM ◽  
JIM C. MacDONALD ◽  
CHRISTIAN H. PONCE ◽  
...  

Campylobacter bacteria are foodborne pathogens that can colonize the gut of food animals. Limited in their ability to ferment sugars, Campylobacter can derive energy for growth via amino acid catabolism. The objectives of the present studies were to test whether supplemental distillers grains containing high amounts of rumen-undegradable intake protein or supplemental lasalocid may, by promoting amino acid flow to the lower bovine gut, increase intestinal carriage of Campylobacter. In study one, 10 steers (5 per treatment) were adapted to diets formulated to achieve 0 or 30% dried distillers grains. After an initial 14-day adaptation to the basal diet, control and treated steers were fed their respective diets for 23 days, after which time they were fed supplemental lasalocid for an additional 8 days, followed by a 5-day withdrawal. In study two, 24 steers preacclimated to a basal diet were adapted via 3-day periodic increases to dietary treatments formulated to achieve 0, 30, or 60% wet corn distillers grains with solubles. Analysis of Campylobacter bacteria cultured from duodenal and fecal samples in study one and from fecal samples in study two revealed no effect of dried distillers grains or wet corn distillers grains with solubles on the prevalence or concentrations of duodenal or fecal Campylobacter. The results from study one indicated that colonized steers, regardless of treatment, harbored higher Campylobacter concentrations when transitioned to the basal diet than when coming off pasture. Campylobacter carriage was unaffected by lasalocid. These results provide no evidence that feeding distillers grains high in rumen-undegradable intake protein or supplemental lasalocid contributes to increased intestinal carriage of Campylobacter in fed cattle.


2020 ◽  
Vol 36 (2) ◽  
pp. 172-182
Author(s):  
W.B. Smith ◽  
M.D. Miller ◽  
W.L. Crossland ◽  
T.R. Callaway ◽  
L.O. Tedeschi ◽  
...  

2014 ◽  
Vol 94 (2) ◽  
pp. 343-347 ◽  
Author(s):  
M. L. He ◽  
L. Xu ◽  
W. Z. Yang ◽  
D. Gibb ◽  
T. A. McAllister

He, M. L., Xu, L., Yang, W. Z., Gibb, D. and McAllister, T. A. 2014. Effect of low-oil corn dried distillers’ grains with solubles on growth performance, carcass traits and beef fatty acid profile of feedlot cattle. Can. J. Anim. Sci. 94: 343–347. The objective of this study was to investigate the effects of dietary inclusion of low-oil corn dried distillers’ grains with solubles (LO-DDGS) on growth, carcass traits and beef fatty acids profiles of finishing feedlot cattle. One hundred and eighty British crossbred steers (450±28.5 kg; six pens/treatment) were offered barley grain-barley silage as the control diet with LO-DDGS replacing barley grain at 200 and 300 g kg−1 dry matter basis in treatment diets. Compared with control, LO-DDGS at 200 g kg−1 did not affect growth performance or carcass traits, whereas at 300 g kg−1 it decreased (P<0.05) gain:feed, but increased (P<0.05) levels of desirable fatty acids in beef. LO-DDGS can replace 200 g kg−1 barley grain in finishing feedlot diets without undesirable impacts on growth performance or carcass traits.


2010 ◽  
Vol 90 (1) ◽  
pp. 87-98 ◽  
Author(s):  
L E McKeown ◽  
A V Chaves ◽  
M Oba ◽  
M E.R. Dugan ◽  
E Okine ◽  
...  

The objective of this research was to determine the effects of increasing proportions of triticale-based dried distillers’ grains with solubles (TDDGS) in the diet on nutrient digestibility, growth and carcass traits of lambs. The control diet contained 72.5% barley grain, 10.0% beet pulp, 9.0% sunflower hulls, 3.0% alfalfa meal and 5.5% mixed supplement, which contained molasses, calcium carbonate, minerals and vitamins. Treatment diets contained TDDGS in place of barley grain at 20, 40 or 60% of diet dry matter (DM). In a growth study, 60 weaned lambs stratified by live weight (26.6 ± 3.6 kg) were fed one of the four diets to slaughter weight. Additionally, a digestibility study using these diets in a replicated 4 × 4 Latin square design was conducted using 12 ram lambs. Increasing dietary allocation of TDDGS in the diet did not affect (P > 0.12) DM intake, average daily gain or feed efficiency (feed:gain). At slaughter, cold carcass weight and grade rule scores responded quadratically (P = 0.04) to increasing TDDGS in the diets. Feeding TDDGS did not affect other carcass traits (P > 0.22). Total saturated fatty acids and monounsaturated fatty acids in subcutaneous fat were unaffected by TDDGS, whereas polyunsaturated fatty acids increased linearly (P = 0.03). Concentration of t10, c12-conjugated linoleic acid (CLA) in carcass fat increased linearly (P = 0.02) with increasing TDDGS, but there was no effect (P ≥ 0.20) on concentrations of c9, t11-CLA or t11-18:1. In the digestibility study, DM intake increased linearly (P < 0.01) with increasing TDDGS in the diet, whereas digestibility of DM, crude protein and fat responded quadratically (P < 0.01). Starch digestibility decreased and ADF digestibility increased linearly (P < 0.01), but there was no treatment effect on NDF digestibility (P > 0.29). Total nitrogen, total phosphorus and soluble phosphorus excretion increased (P < 0.01) with increasing TDDGS. In conclusion, TDDGS can be fed in place of barley grain at levels up to 60% in diets for lambs without adverse affects on growth performance or carcass traits, but this practice will lead to an increase in N and P excretion into the environment.Key words: Lamb, digestibility, triticale distillers grains, performance, carcass characteristics


2008 ◽  
Vol 88 (4) ◽  
pp. 677-684 ◽  
Author(s):  
R. M. Beliveau ◽  
J. J. McKinnon

A trial was conducted to evaluate graded levels of wheat-based dried distillers’ grains with solubles (DDGS) on feedlot performance and carcass characteristics of growing and finishing cattle. Two hundred weaned calves (290 ± 17 kg) were randomly assigned to 1 of 20 pens and fed one of five DDGS treatments. The barley grain-based control diet (0% DDGS) was formulated to 12% crude protein (CP) and 1.52 and 0.93 Mcal kg-1 net energy maintenance (NEm) and net energy gain (NEg) respectively, during the backgrounding period and 1.90 and 1.26 Mcal kg-1 NEm and NEg respectively, during finishing. Wheat-based DDGS replaced (DM basis) barley grain at levels of 8, 16, 24 and 32% during backgrounding and 6, 12, 18 and 23% during finishing. Over the 85-d backgrounding period, dry matter intake (DMI) (P = 0.02), average daily gain (ADG) (P = 0.04), and ultrasound (US) l. dorsi gain (P = 0.02) exhibited a cubic response to DDGS inclusion level with theoretical minima at 6.9, 8.1 and 6.9% DDGS, respectively, and theoretical maxima responses at 27.2, 30.8 and 23.9% DDGS, respectively. Feed efficiency exhibited a quadratic response (P = 0.02) to DDGS inclusion level with a theoretical poorest response at 13.1% DDGS. No effect (P > 0.05) of DDGS inclusion level was noted on ADG, DMI or feed efficiency (kg gain:kg feed; FE) during the finishing period. Similarly, carcass traits were not influenced (P > 0.05) by DDGS inclusion level. The results of this study indicate that wheat-based DDGS can serve as an effective replacement for barley grain in cattle diets supplying both energy and protein and that for finishing cattle, wheat-based DDGS has an energy value at least equal to that of barley grain when fed at levels up to 23% of the diet DM. Key words: Wheat-based dried distillers' grains with solubles, growing and finishing cattle, performance and carcass quality


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