Drought Stress Study on Nicotiana tabacum L., “Baladi”, an In Vitro Experimental Model

Crops drought tolerance is a trait of outmost importance for agriculture especially today when climate change is affecting more the production for food and feed. The scope of this article is to evaluate in vitro drought stress response of Nicotiana tabacum L., “Baladi”. The experiment was set up for four successive stages starting with in vitro seedling development, hypocotyl cultivation, three generations of micropropagation, pre-acclimatization and acclimatization. The effect of abscisic acid (ABA) and/or polyethylene-glycol 6000 (PEG) on tobacco hypocotyl caulogenesis and micropropagation were investigated. Superoxide-dismutases (SODs) and peroxidases (POXs) are more active and different isoforms patterns have been identified compared to the control for cualogenesis. A decrease of internodes length and a higher shoots multiplication rate were observed. However, under PEG treatment plantlets expressed hyperhydration and ceased rooting. Pre-treatments effects study of ABA and/or PEG were finalized in acclimatization phase for 18 tobacco clones. A summary of our results revealed that ABA and/or PEG induce among others a higher oxidative stress compared to the control in the first stage that is not maintained for all clones until acclimatization. Certain clones expressed a lower SOD activity compared to the control during acclimatization but maintaining higher POX activity.

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Studies on the Changes in the Volume and Proliferation Rate of Cells during Embryogenesis of in vitro cultured Pollen Grains of Nicotiana tabacum L.

Zeitschrift für Pflanzenphysiologie ◽

10.1016/s0044-328x(80)80043-2 ◽

1980 ◽

Vol 96 (3) ◽

pp. 261-267 ◽

Cited By ~ 14

Author(s):

Jun Imamura ◽

Hiroshi Harada

Keyword(s):

Nicotiana Tabacum ◽

Pollen Grains ◽

Proliferation Rate ◽

Nicotiana Tabacum L

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Antifungal Prenylated Diphenyl Ethers from Arthrinium arundinis, an Endophytic Fungus Isolated from the Leaves of Tobacco (Nicotiana tabacum L.)

Molecules ◽

10.3390/molecules23123179 ◽

2018 ◽

Vol 23 (12) ◽

pp. 3179 ◽

Cited By ~ 4

Author(s):

Peng Zhang ◽

Xin Li ◽

Xiao-Long Yuan ◽

Yong-Mei Du ◽

Bin-Gui Wang ◽

...

Keyword(s):

Nicotiana Tabacum ◽

Cell Line ◽

Endophytic Fungus ◽

In Vitro Cytotoxicity ◽

Ionization Mass ◽

Monocytic Cell ◽

Monocytic Cell Line ◽

Nicotiana Tabacum L ◽

Diphenyl Ethers

An endophytic fungus Arthrinium arundinis TE-3 was isolated and purified from the fresh leaves of cultivated tobacco (Nicotiana tabacum L.). Chemical investigation on this fungal strain afforded three new prenylated diphenyl ethers (1−3) as well as three known analogues (4−6). Structure elucidation of the isolated compounds was carried out by analysis of 1D and 2D nuclear magnetic resonance (NMR) and high-resolution electrospray ionization mass spectroscopy (HRESIMS) spectra, as well as by comparison of those data with literature data. The absolute configuration of the stereogenic center at C-8 in 1 was assigned by comparison of the experimental and calculated ECD spectra. Compounds 1 and 2 showed selective antifungal activity against Mucor hiemalis with minimum inhibitory concentration (MIC) values of 8 and 4 μg/mL, respectively. Compounds 5 and 6 exhibited inhibitory activity against Alteraria alternata with an MIC value of 8 μg/mL. In the cytotoxic assay, 2, 5, and 6 displayed moderate in vitro cytotoxicity against the human monocytic cell line (THP-1 cell line), with IC50 values of 40.2, 28.3, and 25.9 μM, respectively. This study indicated that endophytic fungi possess great potential for exploring new bioactive secondary metabolites.

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Effect of arginine, putrescine and spermidine on the polyamine, proline and chlorophyll content of tobacco (Nicotiana tabacum L.)

Acta Agraria Debreceniensis ◽

10.34101/actaagrar/2/9572 ◽

2021 ◽

pp. 39-43

Author(s):

Ákos Mendel ◽

László Kovács ◽

Erzsébet Kiss

Keyword(s):

Nicotiana Tabacum ◽

Salt Tolerance ◽

Lower Class ◽

Arginine Decarboxylase ◽

Polyamine Metabolism ◽

Spermine Synthase ◽

Nicotiana Tabacum L ◽

Exogenous Addition ◽

Precursor Molecules

Polyamines, such as spermidine (Spd) spermine (Spm) and their direct precursor, the diamine putrescine (Put) are vital and essential aliphatic amines which are also present in plants. Although ethylene and polyamines are also involved in fruit ripening, the genes coding them must also take part in other biosynthetic pathways. In the ethylene and polyamines play an important role in development of salt stress tolerance, and in responses for biotic and abiotic stresses. Exogenous application of all three main polyamines (Put, Spd, Spm) increase salt tolerance of plants, but, accordingly to previous experiments, spermidine has the main effect on the enhancement of salt tolerance. Nicotiana tabacum L. plants were grown in vitro on MS medium, the treatments were as follows: arginine (150 mg l-1), putrescine (10 mg l-1), spermidine (10 mg l-1). Proline, chlorophyll a, b and polyamine contents were measured. The obtained results show that the arginine decarboxylase and the spermidine synthase genes involved in polyamine metabolism, cannot be enhanced by exogenous addition of their precursor molecules. On the contrary, the spermine synthase gene has a positive effect to the lower-class forms of polyamines.

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In vitro double fertilization in Nicotiana tabacum (L.): polygamy compared with selected single pair somatic protoplast and chloroplast fusions

Sexual Plant Reproduction ◽

10.1007/s004970000044 ◽

2000 ◽

Vol 13 (2) ◽

pp. 113-117 ◽

Cited By ~ 12

Author(s):

Meng-Xiang Sun ◽

Alessandra Moscatelli ◽

Hong-Yuan Yang ◽

M. Cresti

Keyword(s):

Nicotiana Tabacum ◽

Single Pair ◽

Double Fertilization ◽

Nicotiana Tabacum L

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Growth and Division of Single Cells of Higher Plants in Vitro

The Journal of General Physiology ◽

10.1085/jgp.43.4.841 ◽

1960 ◽

Vol 43 (4) ◽

pp. 841-851 ◽

Cited By ~ 169

Author(s):

Ludwig Bergmann

Keyword(s):

Nicotiana Tabacum ◽

Higher Plants ◽

Single Cells ◽

Tissue Cultures ◽

Phaseolus Vulgaris L ◽

Isolated Cells ◽

Nicotiana Tabacum L ◽

Agar Layer ◽

Petri Dishes

The cultivation of single cells of Nicotiana tabacum L. var. "Samsun" and Phaseolus vulgaris L. var. "Early Golden Cluster" on a thin agar layer in Petri dishes is described. Under these conditions about 20 per cent of the cells divided repeatedly and established tissue clones which could be isolated and maintained as growing tissue cultures. It was possible also to follow the successive divisions of isolated cells and to observe their behavior during cytogenesis under the microscope.

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