Comparative Analysis of Differential drought Stress-induced Suppression of and Recovery in Carbon Dioxide Fixation: Stomatal and Non-stomatal Limitation in Nicotiana tabacum L.

1993 ◽  
Vol 142 (3) ◽  
pp. 296-306 ◽  
Author(s):  
Leon van Rensburg ◽  
Gert H.J. Krüger
Keyword(s):  
Carbon Dioxide ◽  
Comparative Analysis ◽  
Drought Stress ◽  
Nicotiana Tabacum ◽  
Carbon Dioxide Fixation ◽  
Stomatal Limitation ◽  
Nicotiana Tabacum L

scholarly journals Transcriptome Profiling, Biochemical and Physiological Analyses Provide New Insights towards Drought Tolerance in Nicotiana tabacum L.

Genes ◽  
2019 ◽  
Vol 10 (12) ◽  
pp. 1041 ◽  
Author(s):  
Rayyan Khan ◽  
Peilu Zhou ◽  
Xinghua Ma ◽  
Lei Zhou ◽  
Yuanhua Wu ◽  
...  
Keyword(s):  
Drought Stress ◽  
Nicotiana Tabacum ◽  
Leaf Anatomy ◽  
Crop Production ◽  
Molecular Mechanisms ◽  
Molecular Level ◽  
Transcriptome Profiling ◽  
Dry Weight ◽  
Antioxidant Defense System ◽  
Nicotiana Tabacum L

Drought stress is one of the main factors limiting crop production, which provokes a number of changes in plants at physiological, anatomical, biochemical and molecular level. To unravel the various mechanisms underpinning tobacco (Nicotiana tabacum L.) drought stress tolerance, we conducted a comprehensive physiological, anatomical, biochemical and transcriptome analyses of three tobacco cultivars (i.e., HongHuaDaJinYuan (H), NC55 (N) and Yun Yan-100 (Y)) seedlings that had been exposed to drought stress. As a result, H maintained higher growth in term of less reduction in plant fresh weight, dry weight and chlorophyll content as compared with N and Y. Anatomical studies unveiled that drought stress had little effect on H by maintaining proper leaf anatomy while there were significant changes in the leaf anatomy of N and Y. Similarly, H among the three varieties was the least affected variety under drought stress, with more proline content accumulation and a powerful antioxidant defense system, which mitigates the negative impacts of reactive oxygen species. The transcriptomic analysis showed that the differential genes expression between HongHuaDaJinYuan, NC55 and Yun Yan-100 were enriched in the functions of plant hormone signal transduction, starch and sucrose metabolism, and arginine and proline metabolism. Compared to N and Y, the differentially expressed genes of H displayed enhanced expression in the corresponding pathways under drought stress. Together, our findings offer insights that H was more tolerant than the other two varieties, as evidenced at physiological, biochemical, anatomical and molecular level. These findings can help us to enhance our understanding of the molecular mechanisms through the networks of various metabolic pathways mediating drought stress adaptation in tobacco.

Comparison of selenite and selenate in alleviation of drought stress in Nicotiana tabacum L.

Chemosphere ◽  
2022 ◽  
Vol 287 ◽  
pp. 132136
Author(s):  
Dan Han ◽  
Shuxin Tu ◽  
Zhihua Dai ◽  
Wuxing Huang ◽  
Wei Jia ◽  
...  
Keyword(s):  
Drought Stress ◽  
Nicotiana Tabacum ◽  
Nicotiana Tabacum L

scholarly journals Drought Stress Study on Nicotiana tabacum L., “Baladi”, an In Vitro Experimental Model

Agriculture ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 845
Author(s):  
Maria-Mihaela Antofie ◽  
Camelia Sava Sand
Keyword(s):  
Drought Stress ◽  
Nicotiana Tabacum ◽  
Multiplication Rate ◽  
Sod Activity ◽  
Nicotiana Tabacum L ◽  
Stress Study ◽  
Food And Feed ◽  
Peg Treatment ◽  
Set Up

Crops drought tolerance is a trait of outmost importance for agriculture especially today when climate change is affecting more the production for food and feed. The scope of this article is to evaluate in vitro drought stress response of Nicotiana tabacum L., “Baladi”. The experiment was set up for four successive stages starting with in vitro seedling development, hypocotyl cultivation, three generations of micropropagation, pre-acclimatization and acclimatization. The effect of abscisic acid (ABA) and/or polyethylene-glycol 6000 (PEG) on tobacco hypocotyl caulogenesis and micropropagation were investigated. Superoxide-dismutases (SODs) and peroxidases (POXs) are more active and different isoforms patterns have been identified compared to the control for cualogenesis. A decrease of internodes length and a higher shoots multiplication rate were observed. However, under PEG treatment plantlets expressed hyperhydration and ceased rooting. Pre-treatments effects study of ABA and/or PEG were finalized in acclimatization phase for 18 tobacco clones. A summary of our results revealed that ABA and/or PEG induce among others a higher oxidative stress compared to the control in the first stage that is not maintained for all clones until acclimatization. Certain clones expressed a lower SOD activity compared to the control during acclimatization but maintaining higher POX activity.

scholarly journals Construction of a genetic linkage map of cigar tobacco (Nicotiana tabacum L.) based on SSR markers and comparative studies

2018 ◽  
Vol 54 (No. 3) ◽  
pp. 115-122 ◽  
Author(s):  
Tong Zhijun ◽  
Xiao Bingguang ◽  
Chen Xuejun ◽  
Fang Dunhuang ◽  
Zhang Yihan ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Nicotiana Tabacum ◽  
Linkage Map ◽  
Ssr Markers ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Average Distance ◽  
Backcross Population ◽  
Breeding Programs ◽  
Nicotiana Tabacum L

Genetic linkage maps representing the tobacco genome have been an important tool for breeding programs because of the elucidation of polygenic traits. We constructed a genetic linkage map of cigar tobacco (Nicotiana tabacum L.) based on an inter-type backcross population of 213 individuals and performed a comparative analysis with other published maps of dark tobacco and flue-cured tobacco. The map consisted of 562 SSR loci distributed on 24 tentative linkage groups and spanned a total length of 1341.18 cM with an average distance of 2.39 cM between adjacent markers. The comparative analysis revealed a Spearman correlation index of 0.93 for marker order conservation with the previously published maps constructed for different tobacco types. Approximately 91% of the SSR markers common to other inter-type maps were located in the same positions as in previous maps. The three maps exhibit good synteny in terms of the shared markers, which suggests that there might be no translocation variations between the genomes of the cigar, dark and flue-cured tobaccos. These results indicate the feasibility of generating a unique genetic map of preferred traits in cigar tobacco and that such mapping may be helpful for breeding programs because plants derived from different inter-type populations can be rapidly scanned using the markers associated with useful cigar traits

Lamellar lipoprotein of Nicotiana chloroplasts: in vivo incorporation of carbon dioxide, acetate, and leucine

1969 ◽  
Vol 47 (5) ◽  
pp. 667-673 ◽  
Author(s):  
Richard S. Bishop ◽  
Mary J. Perry ◽  
Richard W. Schreiber
Keyword(s):  
Carbon Dioxide ◽  
Amino Acids ◽  
Nicotiana Tabacum ◽  
Gel Electrophoresis ◽  
Continuous Flow ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Acetate Incorporation ◽  
Nicotiana Tabacum L

The use of carbon dioxide, acetate, and leucine in the synthesis of lamellar lipoprotein was investigated in chloroplasts of Nicotiana tabacum L. seedlings. These 14C-labelled substrates were fed to sterile seedlings and lamellae were isolated from seedling chloroplasts. The major lamellar lipoprotein was extracted by continuous-flow polyacrylamide gel electrophoresis and the lipoprotein was separated into protein and lipid fractions.All of the substrates were incorporated into both protein and lipid. Carbon dioxide and acetate incorporation was more strongly light dependent than leucine. Light–dark and protein–lipid incorporation ratios were established for each substrate. Leucine label was found in many lamellar amino acids.

Assessing the drought-resistance adaptive advantage of some anatomical and physiological features in Nicotiana tabacum

1994 ◽  
Vol 72 (10) ◽  
pp. 1445-1454 ◽  
Author(s):  
L. van Rensburg ◽  
G. H. J. Kruger ◽  
H. Krüger
Keyword(s):  
Drought Stress ◽  
Nicotiana Tabacum ◽  
Energy Dissipation ◽  
Drought Tolerance ◽  
Rate Constant ◽  
Drought Tolerant ◽  
Nicotiana Tabacum L ◽  
Carotenoid Composition ◽  
Leaf Movements ◽  
Nonradiative Energy Dissipation

As part of an extensive research programme that attempts to explain and quantify the known difference in drought tolerance of four Nicotiana tabacum L. cultivars, some aspects that may contribute towards the avoidance and tolerance of drought stress were investigated to evaluate overall resistance. The results presented emphasize the adaptive significance of effective leaf movements in determining drought tolerance, by reducing or preventing damage to the photosynthetic system caused by photoinhibition and direct heat damage. Leaf movement seemed to be achieved with greater efficiency by the drought-tolerant cultivars GS46 and Elsoma. Drought stress specifically influenced the carotenoid composition. A strong quantitative correlation existed between the formation of zeaxanthin in the xanthophyll cycle and the type of fluorescence quenching, which is indicative of nonradiative energy dissipation. The latter occurred to a lesser extent in the drought-tolerant cultivars GS46 and Elsoma. This phenomenon also indicated that the rate constant for nonradiative energy dissipation in tobacco remains relatively small in relation to the rate constant for fluorescence during drought stress. Furthermore, irrespective of their drought tolerance, it would seem that tobacco plants have a capability towards starch overproduction, though this was less pronounced in the drought-sensitive cultivars TL33 and CDL28. Owing to anatomical differences, resistance to water flow varied between the respective cultivars, as did the percentage intercellular spaces, both of which correlated positively with the slower decrease in water use efficiency and faster recovery upon rehydration of the drought-tolerant cultivars. Key words: carotenoid composition, chlorophyll a fluorescence, Nicotiana tabacum L., mesophyll surface area, leaf movements.

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