scholarly journals Antifungal Activities of Ageratum conyzoides L. Extract against Rice Pathogens Pyricularia oryzae Cavara and Rhizoctonia solani Kühn

Agriculture ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 1169
Author(s):  
Cuong C. Nguyen ◽  
Thanh Q. C. Nguyen ◽  
Kenji Kanaori ◽  
Tran Duy Binh ◽  
Xuyen H. T. Dao ◽  
...  
Keyword(s):  
Rhizoctonia Solani ◽  
Pyricularia Oryzae ◽  
Blast Disease ◽  
Ageratum Conyzoides ◽  
Antifungal Activities ◽  
Sheath Blight Disease ◽  
Trial Testing ◽  
Rice Pathogens ◽  
Blight Disease

Blast disease and sheath blight disease caused by infection with Pyricularia oryzae and Rhizoctonia solani, respectively, are serious fungal diseases in paddy fields. Although synthetic fungicides have been used to control these diseases, the development of ecologically friendly alternatives is required because fungicides can cause health problems and environmental pollution. Natural herbs possessing antifungal activities are among the candidates as alternatives. Ageratum conyzoides is known to contain antifungal compounds, such as precocene II and polymethoxyflavones. Here, we report the antifungal activities of five compounds isolated after ethanol extraction from Ageratum conyzoides against Pyricularia oryzae and Rhizoctonia solani in vitro. Further, we demonstrated the protective effect of the extract on rice from Pyricularia oryzae infection by field trial testing in a shaded net-house.

scholarly journals Uji Efektivitas Rizobakteria Dalam Menghambat Perkembangan Penyakit Hawar Pelepah Daun (Rhizoctonia solani Kuhn.) Pada Padi Secara In Vitro

2020 ◽  
Vol 16 (1) ◽  
pp. 44
Author(s):  
Hanisa Desy Ariani ◽  
Noor Aidawati ◽  
Dewi Arika Adriani
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Rhizoctonia Solani ◽  
Plant Protection ◽  
Block Design ◽  
Sheath Blight ◽  
In Vitro Test ◽  
Sheath Blight Disease ◽  
Randomized Block Design ◽  
Blight Disease

One of the causes of the declining productivity of rice is sheath blight disease caused by the mold Rhizoctonia solani Kuhn. Control of sheath blight disease that is often done by the farmers is by using chemical pesticides (fungicides), which caused environmental problems. One way to reduce the use of pesticides is to biological control by using antagonist bacteria. This study aimed at in vitro test of rhizobacteria in preventing the development of sheath blight disease in rice plants. This research was conducted in the Phytopathology laboratory of Plant Protection Department of Faculty Agriculture, University of Lambung Mangkurat Banjarbaru from March to May 2018. The experiment used a randomized block design with three groups consisting of eight types of rhizobacteria isolates: (r1) Pseudomonas aeruginosa (Barito Kuala), (r2) Bacillus megaterium (Hulu Sungai Tengah), (r3) Azotobacter sp. (Barito Kuala), (r4) Pseudomonas sp. (Hulu Sungai Selatan), (r5) Flavobacterium sp. (Tanah Laut), (r6) Bacillus bodius (Barito Kuala), (r7) Pseudomonas aeruginosa (Hulu Sungai Selatan), (r8) Necercia sp. (Tanah Laut). The results showed that all rhizobacteria have the ability to inhibit the development of R. solani with different percentages of inhibitions. Pseudomonas aeruginosa (Barito Kuala) was the most effective rhizobacteria in inhibiting the development of R. solani.

scholarly journals Biological Control of Sheath Blight Disease of Rice by using Antagonistic Bacteria

1970 ◽  
Vol 45 (3) ◽  
pp. 225-232 ◽  
Author(s):  
MA Bashar ◽  
MA Hossain ◽  
MM Rahman ◽  
MN Uddin ◽  
MN Begum
Keyword(s):  
Biological Control ◽  
Rhizoctonia Solani ◽  
Inhibition Zone ◽  
Sheath Blight ◽  
Bacterial Suspension ◽  
Antagonistic Bacteria ◽  
Causal Organism ◽  
Sheath Blight Disease ◽  
Blight Disease

The study was made to detect and identify antagonistic bacteria to control Rhizoctonia solani, a causal organism of sheath blight (ShB) disease of rice. Isolation of antagonistic bacteria was done from ShB infected rice leaf collected from the districts of Gazipur, Rajshahi, Bogra and Comilla. Fifty isolates of bacteria were isolated. These isolates were tested for antagonism against ShB pathogen of in PDA medium. Among the isolates of antagonistic bacteria (both fluorescent and non fluorescent), eleven produced more than 15 mm inhibition zone, were selected for this study. Remarkable inhibition zone producing ten isolates were selected to observe their antagonistic behaviour by soaking the sclerotia of Rhizoctonia solani and rice seedlings in different hours into bacterial suspension of 3.84 x 107 CFU/ml. Both the in vitro and in vivo rom showed that the sclerotial germination and sheath blight symptom expression were delayed 6-9 days. Soaking of both seedlings and sclerotia with the test bacteria was found most effective to control ShB (38% - 100%) than soaking of seedlings alone with bacterial suspension at different hours. However, BanShbFPS5 (2)B, BanShb738(3), BanShb738(2) and BanShb581(1), the four antagonistic bacterial isolates could be applied as biological agent to control sheath blight disease of rice and they could control sheath blight disease development and could delay the epidemics of the disease. Key words: Biological control; Sheath blight disease; Rhizoctonia solani; Antagonistic bacteria DOI: 10.3329/bjsir.v45i3.6529Bangladesh J. Sci. Ind. Res. 45(3), 225-232, 2010

scholarly journals In vitro and in vivo antifungal properties of silver nanoparticles against Rhizoctonia solani , a common agent of rice sheath blight disease

2016 ◽  
Vol 11 (3) ◽  
pp. 236-240 ◽  
Author(s):  
Meysam Soltani Nejad ◽  
Gholam Hosein Shahidi Bonjar ◽  
Mehrdad Khatami ◽  
Abbas Amini ◽  
Sonia Aghighi
Keyword(s):  
Silver Nanoparticles ◽  
Rhizoctonia Solani ◽  
Sheath Blight ◽  
Rice Sheath Blight ◽  
Sheath Blight Disease ◽  
Antifungal Properties ◽  
Blight Disease

scholarly journals Biological Control of Rice Sheath Blight Disease (Rhizoctonia solani) Using Bio-pesticides and Bio-control Agents

2021 ◽  
Vol 24 (1) ◽  
pp. 47-58
Author(s):  
MM Rashid ◽  
MR Bhuiyan ◽  
HA Dilzahan ◽  
MA Hamid ◽  
N Hasan ◽  
...  
Keyword(s):  
Disease Control ◽  
Rhizoctonia Solani ◽  
Trichoderma Harzianum ◽  
Sheath Blight ◽  
Dual Culture ◽  
Soil Application ◽  
Sheath Blight Disease ◽  
Mycelia Growth ◽  
Blight Disease

A series of experiments were conducted in Plant Pathology Laboratory at Bangladesh Rice Research Institute (BRRI), Gazipur to know the efficacy of four bio-pesticides Recharge, Microtech1, Agroplus and Chitin and two bio-control agents (Trichoderma harzianum and Bacillus subtilis) on the major rice disease sheath blight (ShB) caused by Rhizoctonia solani in in-vitro and field condition. One fungicide Nativo (Tebuconazole 50% + Trifloxystrobin 25%) and one disease control (without any treatments) were also included as treatment for comparison. Radial mycelia growth of R. solani was mostly inhibited by a Bangladesh Trichoderma harzianum (BT1), Recharge and chemical fungicide Nativo 75 G at 48 hours of dual contact of R. solani and tested bio-pesticides or bio-control agents in in-vitro. The other bio-pesticides along with B. subtilis didn’t show any inhibition effect on the mycelia growth of R. solani which was similar to control (only water) treatment. Fungal inhibition 87.5% and 86.3% of Rhizoctonia solani was obtained by Recharge and Trichoderma harzianum (BT1), respectively which was nearly similar to the chemical treatment Nativo (96.3%) in in-vitro dual culture method compared to control treatment. In net house condition, 74% sheath blight disease was inhibited by soil application of Recharge (0.3 g/m2 with 50 ml water) for two times at the time of transplanting and 30 days after transplanting (DAT), whereas similar soil application of B. subtilis (OD600=0.3) was not found effective to control the ShB disease (< 30% disease reduction). The highest ShB disease was reduced by the chemical control Nativo 75 G (94.2%) followed by BT1 (89%), bio-pesticide Recharge (70.8%), Microtech1 (37.4%) and Chitin (61.3%) compared with the disease control when the tested materials were sprayed for two times (3 days before and 4 days after inoculation). Bangladesh Rice J. 24 (1): 47-58, 2020

scholarly journals BIOLOGICAL CONTROL OF BANDED LEAF AND SHEATH BLIGHT DISEASE (Rhizoctonia solani KUHN) IN CORN WITH FORMULATED Bacillus subtilis BR23

2016 ◽  
Vol 7 (1) ◽  
pp. 1 ◽  
Author(s):  
Amran Muis ◽  
Arcadio J. Quimio
Keyword(s):  
Bacillus Subtilis ◽  
Seed Germination ◽  
Rhizoctonia Solani ◽  
Seed Treatment ◽  
The Philippines ◽  
Sheath Blight ◽  
Resistant Variety ◽  
Sodium Hypochlorite Solution ◽  
Sheath Blight Disease ◽  
Blight Disease

Rhizoctonia solani Kuhn. causing banded leaf and sheath blight diseases is one of the important fungi of corn world wide. The fungus is commonly controlled by using fungicide because no resistant variety available. The objective of the study was to develop a seed treatment formulation of the selected Bacillus subtilis to control R. solani in corn. The study was conducted in the Department of Plant Pathology, College of Agriculture, University of the Philippines Los Bañòs, College, Laguna from May 2004 to August 2005, using sweet corn var. IPB Supersweet as test plant. Corn seeds were surface sterilized for 10 minutes in 1% sodium hypochlorite solution and 5% ethanol, washed thrice with sterile distilled water and air-dried. The seeds were coated with formulated B. subtilis BR23 and used for several experiments, such as evaluation for their germination and growth in the laboratory, effectively on R. solani in the baked and nonbaked field soil under greenhouse condition, and in the microplots artificially infested with R. solani. The treatment was compared with other standard seed treatment of synthetic fungicides such as captan (10 g per kg seeds) and metalaxyl (10 g per kg seeds). The experiments were designed in a completely random design with three replications. Parameters observed were seed germination, plant height, disease scores, and plant yield. Laboratory formulated B. subtilis BR23 used as seed treatment had no detrimental effects on seed germination and seedling vigor. In microplots artificially infested with a selected highly virulent R. solani, seed treatment with the same formulation increased grain yield by 27% compared to that of the control captan seed treatment with 14.4%. The studies showed the potential of B. subtilis BR23 for commercialization as a seed treatment for the control of banded leaf and sheath blight disease (R. solani) in corn.

Antifungal Activities of Some Indole Derivatives

2010 ◽  
Vol 65 (7-8) ◽  
pp. 437-439 ◽  
Author(s):  
Hui Xu ◽  
Qin Wang ◽  
Wen-Bin Yang
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Graminearum ◽  
Broad Spectrum ◽  
Alternaria Alternata ◽  
Phytopathogenic Fungi ◽  
Pyricularia Oryzae ◽  
Indole Derivatives ◽  
Alternaria Brassicae ◽  
Antifungal Activities

Nine indole derivatives were evaluated in vitro against Fusarium graminearum, Alternaria alternata, Helminthosporium sorokinianum, Pyricularia oryzae, Fusarium oxysporum f. sp. vasinfectum, Fusarium oxysporum f. sp. cucumarinum, and Alternaria brassicae. Most of the compounds were found to possess antifungal activities. Especially compounds 2, 5, 8, and 9 exhibited broad-spectrum antifungal activities against the above-mentioned seven phytopathogenic fungi, and showed more potent activities than hymexazole, a commercial agricultural fungicide.

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