scholarly journals In Vitro Propagation Strategies of Medicinally Important Berry Crop, Lingonberry (Vaccinium vitis-idaea L.)

Agronomy ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 744
Author(s):  
Samir C. Debnath ◽  
Usha Arigundam
Keyword(s):  
In Vitro Propagation ◽  
Genetic Characteristics ◽  
Current Review ◽  
Small Fruit ◽  
Antioxidant Metabolites ◽  
Health Promoting ◽  
Micropropagated Plants ◽  
Conventional Methods ◽  
Seed Propagation

Lingonberry (Vaccinium vitis-idaea L.) is a health-promoting small fruit crop rich in antioxidant metabolites that helps to reduce the incidence of degenerative diseases. Being heterozygous, lingonberries cannot preserve genetic characteristics through seed propagation. Conventional vegetative propagation, although it produces true-to-type plants, is not economically viable. In vitro propagation can multiply plants much faster than conventional methods. A liquid cultures system under a bioreactor micropropagation system is of significant importance to increase the multiplication rates of in vitro-produced shoots. Enhanced vegetative growth and variation in biochemical constituents are observed in micropropagated plants. Clonal fidelity, although it may be a serious problem for commercial micropropagation, can be verified efficiently by molecular markers. The current review provides detailed and updated information on lingonberry micropropagation along with conventional methods and their effects on morphological, molecular and biochemical characteristics in micropropagated plants, filling the gap in literature.

scholarly journals Micropropagation, seed propagation and germplasm bank of Mandevilla velutina (Mart.) Woodson

2007 ◽  
Vol 64 (3) ◽  
pp. 263-268 ◽  
Author(s):  
Ronaldo Biondo ◽  
Ana Valéria Souza ◽  
Bianca Waléria Bertoni ◽  
Andreimar Martins Soares ◽  
Suzelei Castro França ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Plant Species ◽  
In Vitro Propagation ◽  
Nodal Segments ◽  
Medicinal Plant Species ◽  
Soil Conditions ◽  
Ms Medium ◽  
Germplasm Bank ◽  
Seed Propagation

Mandevilla velutina (Mart.) Woodson (Apocynaceae) is a medicinal plant species with antivenom properties, native from Brazilian Savanna regions (Cerrado), which due to overexploitation and habitat deforestation is in danger of extinction. As an initiative for conserving this endangered but economically important plant species, a micropropagation protocol was developed and genotypes were stored in the Germplasm Bank "Cerrado In vitro". For the in vitro propagation of M. velutina, nodal segments were inoculated on Murashige and Skoog (MS) medium supplemented with different concentrations of BA, Zeatin, 2ip, DTT and TDZ. Best multiplication ratio was achieved when to the medium 0.44 µM BA, ranging 1: 6.7, were added. Plantlets cultured on MS/2 medium supplemented with 26.85 µM NAA rooted successfully (50.5%). Although rooted and un-rooted plantlets acclimatized to soil conditions, great losses were observed within un-rooted plantlets, while the rooted presented 100 % survival. It was possible to maintain 43% of the M. velutina germplasm under healthy conditions for six months, with no subcultures, using the MS medium supplemented with 2% sucrose, 13.8 mM spermidine, 2% sorbitol and 2% dextrose.

scholarly journals In vitro Propagation of Plectranthus bourneae Gamble? An Endemic Red Listed Plant

2016 ◽  
Vol 25 (2) ◽  
pp. 273-284 ◽  
Author(s):  
R Thaniarasu ◽  
T Senthil Kumar ◽  
MV Rao
Keyword(s):  
Tamil Nadu ◽  
In Vitro Propagation ◽  
Natural Populations ◽  
Shoot Multiplication ◽  
Multiple Shoot ◽  
Effective Concentration ◽  
Shoot Length ◽  
Micropropagated Plants ◽  
Plectranthus Bourneae

An efficient protocol of in vitro propagation of Plectranthus bourneae Gamble (Lamiaceae), a valuable medicinal important and endemic Red listed plant of Western Ghats, (Tamil Nadu, India) was standardized by improved shoot multiplication from axillary bud explant. An in vitro propagation system has been reconnoitered on MS with the effective concentration BA (0.7 mg/l) followed by a combination of BA (0.7 mg/l) and TDZ (1.0 mg/l) which promoted high number of shoots. The multiple shoot rate was enhanced further by adding AdS (50 mg/l). Beneficial shoot length was achieved when cultured on MS containing GA3 (0.5 mg/l). Rooting was increased on MS augmented with IBA (1.5 mg/l). Micropropagated plants were acclimatized and the survival rate was 80%. Acclimatized P. bourneae plants can be used as substitute alternative to natural populations. Using this protocol the propagated plants can be used for conservation strategies.Plant Tissue Cult. & Biotech. 25(2): 273-284, 2015 (December)

scholarly journals Epigenomic Insight of Lingonberry: Health Promoting Trait Under Micropropagation

2021 ◽  
Author(s):  
Arindam Sikdar ◽  
Umanath Sharma ◽  
Rajesh Barua ◽  
Abir U. Igamberdiev ◽  
Samir C. Debnath
Keyword(s):  
Tissue Culture ◽  
Secondary Metabolites ◽  
Cytosine Methylation ◽  
Phenolic Content ◽  
Secondary Compounds ◽  
Total Phenolic ◽  
Health Promoting ◽  
Micropropagated Plants

Abstract Epigenetic variation plays a role in developmental gene regulation and responses to the environment. An efficient interaction of zeatin induced cytosine methylation and secondary compounds has been displayed for the first time in tissue-culture shoots of lingonberry (Vaccinium vitis-idaea) in vitro, in vivo and its cutting-cultivar Erntedank. Through MSAP assay, we observed highest methylated sites in leaf regenerants (LC1) from all primer combinations (108 bands), with their highest variation in secondary metabolites. We measured that four tissue-culture plants showed higher methylation bands than cutting propagated donor plants (ED) which exhibited 79 bands of methylation, which is comparatively low. On the other hand, we observed the highest total phenolic content in node culture-derived greenhouse grown plants, NC3 but leaf culture-derived greenhouse grown plants, LC1 represented low phenolic content. Our study showed more methylation in micropropagated plants (NC1, NC2, NC3, LC1) than those derived from cutting propagated ED plants, where methylation was not present. On the contrary, we observed higher secondary metabolites in ED plants but comparatively less in micropropagated shoots (NC1, NC2) and plants (NC3, LC1). Our study displayed that higher methylation sites observed in micropropagated plants possessed less amount of secondary metabolites.

scholarly journals Field Performance and Genetic Stability of Micropropagated Gooseberry Plants (Ribes grossularia L.)

Agronomy ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 45
Author(s):  
Danuta Wójcik ◽  
Aleksandra Trzewik ◽  
Danuta Kucharska
Keyword(s):  
Genetic Stability ◽  
Fragment Length Polymorphism ◽  
Field Performance ◽  
Inter Simple Sequence Repeat ◽  
Planting Material ◽  
Small Fruit ◽  
Micropropagated Plants ◽  
Growth Vigor ◽  
Simple Sequence

Gooseberry (Ribes grossularia L.) is a small fruit crop producing valuable fruits, which is constantly gaining importance. In vitro propagation of this species can significantly support the production of virus-free planting material and accelerate the introduction of new cultivars to the market. The aim of presented study was to assess field performance and genetic stability of micropropagated plants (MPs) of four gooseberry cultivars, “Captivator”, “Hinnonmaki Rot”, “Invicta”, and “Resika”. The growth vigor and yield of MPs and plants propagated by standard methods from softwood cuttings (ST) were evaluated in a field experiment. Microscopic observations of the number and length of the stomata of MP and ST plants were carried out. Two DNA-based techniques, amplified fragment length polymorphism (AFLP) and inter simple sequence repeat (ISSR), were used to assess genetic stability of MP plants. For analysis of genetic stability of ST plants, the ISSR technique was applied. For three cultivars, Captivator, Hinnonmaki Rot, and Invicta, the plants’ growth vigor and fruit yield were greater in MP plants than in ST plants. In the case of Resika, most of these parameters were higher in ST plants. Microscopic observations of the stomata indicated a lack of differences in the length between MP and ST plants, while the stomata frequency on leaves of MP plants was higher than that of ST plants. The genetic variability of MP plants, assessed by AFLP, ranged from 0.35% for Hinnonmaki Rot to 2.12% for Resika. The results of ISSR analysis of MP plants showed variability from 0% in the case of Hinnonmaki Rot and Resika to 4% and 8.69% for Captivator and Invicta, respectively. No polymorphism was detected among ST plants of all analyzed gooseberry cultivars.

scholarly journals Efficient in vitro propagation of Artemisia nilagirica var. nilagirica (Indian wormwood) and assessment of genetic fidelity of micropropagated plants

2016 ◽  
Vol 22 (4) ◽  
pp. 595-603 ◽  
Author(s):  
Smita Shinde ◽  
Joseph Kadanthottu Sebastian ◽  
Jyothi Ramesh Jain ◽  
Manohar Shirugumbi Hanamanthagouda ◽  
Hosakatte Niranjana Murthy
Keyword(s):  
In Vitro Propagation ◽  
Genetic Fidelity ◽  
Artemisia Nilagirica ◽  
Micropropagated Plants

scholarly journals In Vitro Propagation and Variation of Antioxidant Properties in Micropropagated Vaccinium Berry Plants—A Review

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 788 ◽  
Author(s):  
Samir C. Debnath ◽  
Juran C. Goyali
Keyword(s):  
Antioxidant Activity ◽  
In Vitro Propagation ◽  
Ex Vivo ◽  
Antioxidant Properties ◽  
Morphological Differentiation ◽  
Berry Crops ◽  
Total Antioxidant ◽  
Antioxidant Metabolites ◽  
Propagation Methods

The berry crops in genus Vacciniun L. are the richest sources of antioxidant metabolites which have high potential to reduce the incidence of several degenerative diseases. In vitro propagation or micropropagation has been attractive to researchers for its incredible potential for mass production of a selected genotype in a short time, all year round. Propagation techniques affect the antioxidant activity in fruits and leaves. Total antioxidant activity was higher in the fruit of in vitro propagated plants compare to the plants grown ex vivo. This review provides critical information for better understanding the micropropagation and conventional propagation methods, and their effects on antioxidant properties and morphological differentiation in Vaccinium species, and fills an existing gap in the literature.

scholarly journals Micropropagation, Genetic Fidelity and Phenolic Compound Production of Rheum rhabarbarum L.

Plants ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 656
Author(s):  
Doina Clapa ◽  
Orsolya Borsai ◽  
Monica Hârța ◽  
Victoriţa Bonta ◽  
Katalin Szabo ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Antioxidant Properties ◽  
Genetic Fidelity ◽  
Bioactive Compound ◽  
Mother Plant ◽  
Medicinal Species ◽  
Planting Material ◽  
Micropropagated Plants ◽  
Polyphenolic Profile

An efficient micropropagation protocol for Rheum rhabarbarum L. was developed in this study. The in vitro rhubarb plants obtained in the multiplication stage (proliferation rate: 5.0 ± 0.5) were rooted in vitro (96% rooting percentage) and acclimatized ex vitro in floating perlite, with 90% acclimatization percentage. To assess the genetic fidelity between the mother plant and in vitro propagated plants, sequence-related amplified polymorphism (SRAP) markers were used. All banding profiles from the micropropagated plants were monomorphic and similar to those of the mother plant indicating 100% similarity. Regarding the polyphenolic profile, gallic, protocatechuic, p-hydroxybenzoic, vanillic, chlorogenic, caffeic, syringic, p-coumaric and ferulic acid were present in different amounts (2.3–2690.3 μg g−1 dry plant), according to the extracted matrix. Aglicons and glycosides of different classes of flavonoids were also identified. The rhizome extracts (both from in vitro and field grown plants) contained resveratrol, a stilbene compound with high antioxidant properties, ranging between 229.4 to 371.7 μg g−1 plant. Our results suggest that in vitro propagation of Rheum rhabarbarum L. represents a reliable alternative to obtain a large number of true-to-type planting material with high bioactive compound content of this valuable nutritional and medicinal species.

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