scholarly journals Resistance to Three Distinct Begomovirus Species in the Agronomical Superior Tropical Pumpkin Line AVPU1426 Developed at the World Vegetable Center

Agronomy ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1256
Author(s):  
Narinder P. S. Dhillon ◽  
Supornpun Srimat ◽  
Suwannee Laenoi ◽  
Anjana Bhunchoth ◽  
Bencharong Phuangrat ◽  
...  
Keyword(s):  
Recessive Gene ◽  
Field Tests ◽  
New Delhi ◽  
Carotene Content ◽  
F1 Hybrid ◽  
The World ◽  
Pedigree Selection ◽  
Yellow Flesh ◽  
Tomato Leaf Curl ◽  
Leaf Curl

The Squash Leaf Curl China Virus (SLCCNV) and Tomato Leaf Curl New Delhi Virus (ToLCNDV) are species of Begomovirus (whitefly-vectored Geminiviridae) and cause serious damage to the cucurbit crops of the genus Cucurbita in the areas of South and Southeast Asia, across Asia, the Middle East and the Mediterranean, respectively. Cucurbita moschata’s inbred line AVPU1426, developed at the World Vegetable Center (WorldVeg) from a Bangladeshi landrace through pedigree selection, was observed to be resistant to both begomoviruses in field tests conducted at the WorldVeg Research and Training Station, Kasetsart University, Kamphaeng Saen, Thailand, which is a hotspot for these viruses. When AVPU1426 was tested for reaction to inoculation by viruliferous whiteflies with Squash Leaf Curl Philippines Virus (Taiwan strain) (SLCPV-TW) in the screen net-house at WorldVeg headquarters in Taiwan, it showed good resistance, though SLCPV-TW DNA-A could be detected in all inoculated plants, indicating that it did not show immunity to this virus. The objective of this study was to validate the resistance to SLCCNV and ToLCNDV in AVPU1426 by using whitefly-mediated inoculations to determine the mode of inheritance of the resistance. The results showed that AVPU1426 was resistant to SLCCNV and ToLCNDV. Upon crossing AVPU1426 with a susceptible check variety, Waltham Butternut, the resistance to both begomoviruses was observed to be conferred by a single recessive gene. This open-pollinated pumpkin line AVPU1426 bears flat round, mottled green immature fruits with yellow flesh in mature fruit. The fruit yield of AVPU1426 (20.74 t/ha−1) was comparable to ‘Rajah’ (18.61 t/ha−1), a recently released commercial F1 hybrid of East-West Seed (EWS). The fruit of AVPU1426 were estimated to have a good average β-carotene content (1.57 mg/100 g fresh weight). This line is a good source to breed pumpkins resistant to the three begomoviruses.

scholarly journals Resistance Against Melon Chlorotic Mosaic Virus and Tomato Leaf Curl New Delhi Virus in Melon

Plant Disease ◽  
2019 ◽  
Vol 103 (11) ◽  
pp. 2913-2919 ◽  
Author(s):  
Gustavo Romay ◽  
Michel Pitrat ◽  
Herve Lecoq ◽  
Catherine Wipf-Scheibel ◽  
Pauline Millot ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Virus Resistance ◽  
Dominant Gene ◽  
Virus Detection ◽  
Recessive Gene ◽  
Tomato Leaf ◽  
Enzyme Linked Immunosorbent Assay ◽  
New Delhi ◽  
Tomato Leaf Curl ◽  
Leaf Curl

Thirty-one melon accessions were screened for resistance to the begomoviruses Melon chlorotic mosaic virus (MeCMV) and Tomato leaf curl New Delhi virus (ToLCNDV). Five accessions presented nearly complete resistance to both viruses. Accession IC-274014, showing the highest level of resistance to both viruses, was crossed with the susceptible cultivar Védrantais. The F1, F2, F3/F4, and both backcross progenies were mechanically inoculated with MeCMV. Plants without symptoms or virus detection by enzyme-linked immunosorbent assay and/or PCR were considered as resistant. The segregations were compatible with two recessive and one dominant independent genes simultaneously required for resistance. Inheritance of resistance to ToLCNDV in the F2 was best explained by one recessive gene and two independent dominant genes simultaneously required. Some F3 and F4 families selected for resistance to MeCMV also were resistant to ToLCNDV, suggesting that common or tightly linked genes were involved in resistance to both viruses. We propose the names begomovirus resistance-1 and Begomovirus resistance-2 for these genes (symbols bgm-1 and Bgm-2). Resistance to MeCMV in IC-274014 was controlled by bgm-1, Bgm-2, and the recessive gene melon chlorotic mosaic virus resistance (mecmv); resistance to ToLCNDV was controlled by bgm-1, Bgm-2, and the dominant gene Tomato leaf curl New Delhi virus resistance (Tolcndv).

Potato apical leaf curl disease: current status and perspectives on a disease caused by tomato leaf curl New Delhi virus

2021 ◽  
Author(s):  
Ravinder Kumar ◽  
Rahul Kumar Tiwari ◽  
Arjunan Jeevalatha ◽  
Sundaresha Siddappa ◽  
Mohd. Abas Shah ◽  
...  
Keyword(s):  
Tomato Leaf ◽  
Current Status ◽  
New Delhi ◽  
Apical Leaf ◽  
Tomato Leaf Curl ◽  
Leaf Curl Disease ◽  
Leaf Curl

Tomato leaf curl New Delhi virus. [Distribution map].

2016 ◽  
Author(s):  
Keyword(s):  
Tamil Nadu ◽  
Uttar Pradesh ◽  
Tomato Leaf ◽  
New Delhi ◽  
Distribution Map ◽  
Tomato Leaf Curl ◽  
Virus Distribution ◽  
New Distribution ◽  
Distribution In Europe ◽  
Leaf Curl

Abstract A new distribution map is provided for Tomato leaf curl New Delhi virus. Geminiviridae: Begomovirus. Hosts: tomato (Solanum lycopersicum) and other Solanaceae such as aubergine (S. melongena), potato (S. tuberosum), Capsicum spp. and Cucurbitaceae. Information is given on the geographical distribution in Europe (Italy, Sicily, Spain, Mainland Spain), Asia (Bangladesh, India, Andhra Pradesh, Delhi, Gujarat, Haryana, Karnataka, Maharashtra, Punjab, Tamil Nadu, Uttar Pradesh, West Bengal, Indonesia, Java, Iran, Pakistan, Philippines, Sri Lanka, Taiwan and Thailand) and Africa (Tunisia).

Diagnostic methods of tomato leaf curl New Delhi virus in Russian Federation

2021 ◽  
Author(s):  
E. Lozovaya ◽  
Y. Prikhodko ◽  
T. Zhivaeva ◽  
E. Karimova ◽  
Y. Shneyder
Keyword(s):  
Russian Federation ◽  
Tomato Leaf ◽  
Diagnostic Methods ◽  
New Delhi ◽  
Tomato Leaf Curl ◽  
Leaf Curl

Emergence of tomato leaf curl New Delhi virus in Italy: estimation of incidence and genetic diversity

2019 ◽  
Vol 68 (3) ◽  
pp. 601-608 ◽  
Author(s):  
S. Panno ◽  
A. G. Caruso ◽  
E. Troiano ◽  
M. Luigi ◽  
A. Manglli ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Tomato Leaf ◽  
New Delhi ◽  
Tomato Leaf Curl ◽  
Leaf Curl

Diversity analysis of Tomato leaf curl New Delhi virus-[potato], causing apical leaf curl disease of potato in India

2017 ◽  
Vol 45 (1) ◽  
pp. 33-43 ◽  
Author(s):  
Arjunan Jeevalatha ◽  
Swarup Kumar Chakrabarti ◽  
Sanjeev Sharma ◽  
Vinay Sagar ◽  
Kamlesh Malik ◽  
...  
Keyword(s):  
Tomato Leaf ◽  
Diversity Analysis ◽  
New Delhi ◽  
Apical Leaf ◽  
Tomato Leaf Curl ◽  
Leaf Curl Disease ◽  
Leaf Curl

scholarly journals First report of tomato leaf curl New Delhi virus in zucchini crops in Greece

2019 ◽  
Vol 101 (3) ◽  
pp. 799-799 ◽  
Author(s):  
Chrysoula G. Orfanidou ◽  
Ioanna Malandraki ◽  
Despoina Beris ◽  
Oxana Kektsidou ◽  
Nikon Vassilakos ◽  
...  
Keyword(s):  
Tomato Leaf ◽  
New Delhi ◽  
First Report ◽  
Tomato Leaf Curl ◽  
Leaf Curl

scholarly journals In silico evaluation of molecular virus–virus interactions taking place between Cotton leaf curl Kokhran virus- Burewala strain and Tomato leaf curl New Delhi virus

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12018
Author(s):  
Nida Fatima Ali ◽  
Rehan Zafar Paracha ◽  
Muhammad Tahir
Keyword(s):  
Protein Structures ◽  
Protein Docking ◽  
Tomato Leaf ◽  
Replication Initiation ◽  
Electrostatic Energy ◽  
Cotton Leaf ◽  
New Delhi ◽  
Virus Interactions ◽  
Tomato Leaf Curl ◽  
Leaf Curl

Background Cotton leaf curl disease (CLCuD) is a disease of cotton caused by begomoviruses, leading to a drastic loss in the annual yield of the crop. Pakistan has suffered two epidemics of this disease leading to the loss of billions in annual exports. The speculation that a third epidemic of CLCuD may result as consequence of the frequent occurrence of Tomato leaf curl New Delhi virus (ToLCNDV) and Cotton leaf curl Kokhran Virus-Burewala Strain (CLCuKoV-Bu) in CLCuD infected samples, demand that the interactions taking between the two viruses be properly evaluated. This study is designed to assess virus-virus interactions at the molecular level and determine the type of co-infection taking place. Methods Based on the amino acid sequences of the gene products of both CLCuKoV-Bu and ToLCNDV, protein structures were generated using different software, i.e., MODELLER, I-TASSER, QUARKS, LOMETS and RAPTORX. A consensus model for each protein was selected after model quality assessment using ERRAT, QMEANDisCo, PROCHECK Z-Score and Ramachandran plot analysis. The active and passive residues in the protein structures were identified using the CPORT server. Protein–Protein Docking was done using the HADDOCK webserver, and 169 Protein–Protein Interaction (PPIs) were performed between the proteins of the two viruses. The docked complexes were submitted to the PRODIGY server to identify the interacting residues between the complexes. The strongest interactions were determined based on the HADDOCK Score, Desolvation energy, Van der Waals Energy, Restraint Violation Energy, Electrostatic Energy, Buried Surface Area and Restraint Violation Energy, Binding Affinity and Dissociation constant (Kd). A total of 50 ns Molecular Dynamic simulations were performed on complexes that exhibited the strongest affinity in order to validate the stability of the complexes, and to remove any steric hindrances that may exist within the structures. Results Our results indicate significant interactions taking place between the proteins of the two viruses. Out of all the interactions, the strongest were observed between the Replication Initiation protein (Rep) of CLCuKoV-Bu with the Movement protein (MP), Nuclear Shuttle Protein (NSP) of ToLCNDV (DNA-B), while the weakest were seen between the Replication Enhancer protein (REn) of CLCuKoV-Bu with the REn protein of ToLCNDV. The residues identified to be taking a part in interaction belonged to domains having a pivotal role in the viral life cycle and pathogenicity. It maybe deduced that the two viruses exhibit antagonistic behavior towards each other, and the type of infection may be categorised as a type of Super Infection Exclusion (SIE) or homologous interference. However, further experimentation, in the form of transient expression analysis, is needed to confirm the nature of these interactions and increase our understanding of the direct interactions taking place between two viruses.

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