scholarly journals Effects of Syo-seiryu-to and Its Constituent Crude Drugs on Phorbol Ester-Induced Up-Regulation of IL-33 and Histamine H1 Receptor mRNAs in Swiss 3T3 and HeLa Cells

Allergies ◽  
2021 ◽  
Vol 1 (3) ◽  
pp. 163-175
Author(s):  
Seiichi Nakano ◽  
Sayaka Yamamoto ◽  
Takako Esu ◽  
Shiho Naniwa ◽  
Yuki Konishi ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Nasal Congestion ◽  
Eosinophil Infiltration ◽  
High Concentration ◽  
H1 Receptor ◽  
Traditional Herbal Medicine ◽  
Signal Molecule ◽  
Histamine H1 Receptor ◽  
Crude Drugs

Syo-seiryu-to (SST) is a traditional herbal medicine that has been used clinically to treat allergic rhinitis (AR) in Japan. SST improves acute symptoms, such as sneezing and rhinorrhea, as well as chronic symptoms, such as nasal obstruction, in patients with AR. However, its therapeutic mechanisms remain unknown. We examined the effects of SST and eight constituent crude drugs on phorbol 12-myristate-13-acetate (PMA)-induced gene up-regulation of IL-33 and histamine H1 receptor (H1R), which are responsible for the pathogenesis of AR. We found that SST and its crude drugs, except for Pinellia tuber, significantly and dose-dependently suppressed PMA-induced both IL-33 and H1R mRNA up-regulation in vitro. The half-maximal inhibitory concentration values of the seven crude drugs to inhibit PMA-induced IL-33 mRNA up-regulation were correlated with those related to H1R mRNA up-regulation, suggesting that they act on a common signal molecule. These results suggest that SST improves nasal congestion that is induced by IL-33-related eosinophil infiltration and inhibits sneezing and rhinorrhea that are induced by H1R-mediated histamine signaling in the nasal mucosa of AR patients through its inhibition of a common molecule in the gene expression pathways of IL-33 and H1R. The results could explain the advantages of traditional herbal medicine, in which mixing various crude drugs not only acts on a common target to enhance its pharmacological action, similar to the effect of a high concentration of a single crude extract but also has the benefit of reducing the side effects of each crude drug.

scholarly journals Rikkunshito Ameliorates Cancer Cachexia Partly through Elevation of Glucarate in Plasma

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Katsuya Ohbuchi ◽  
Shin Nishiumi ◽  
Naoki Fujitsuka ◽  
Tomohisa Hattori ◽  
Masahiro Yamamoto ◽  
...  
Keyword(s):  
Weight Loss ◽  
Herbal Medicine ◽  
Rat Model ◽  
Cancer Cachexia ◽  
Host Cells ◽  
Metabolome Analysis ◽  
Traditional Herbal Medicine ◽  
Delayed Onset ◽  
Tumor Bearing

Cancer cachexia, which is characterized by decreased food intake, weight loss and systemic inflammation, increases patient’s morbidity and mortality. We previously showed that rikkunshito (RKT), a Japanese traditional herbal medicine (Kampo), ameliorated the symptoms of cancer cachexia through ghrelin signaling-dependent and independent pathways. To investigate other mechanisms of RKT action in cancer cachexia, we performed metabolome analysis of plasma in a rat model bearing the Yoshida AH-130 hepatoma. A total of 110 metabolites were detected in plasma and RKT treatment significantly altered levels of 23 of those metabolites in cachexia model rats. Among them, glucarate, which is known to have anticarcinogenic activity through detoxification of carcinogens via inhibition ofβ-glucuronidase, was increased in plasma following administration of RKT. In our AH-130 ascites-induced cachexia rat model, administration of glucarate delayed onset of weight loss, improved muscle atrophy, and reduced ascites content. Additionally, glucarate reduced levels of plasma interferon-γ(IFN-γ) in tumor-bearing rats and was also found to suppress LPS-induced IFN-γexpression in splenocytesin vitro. These results suggest that glucarate has anti-inflammatory activity via a direct effect on immune host cells and suggest that RKT may also ameliorate inflammation partly through the elevation of glucarate in plasma.

scholarly journals Traditional Herbal Medicine, Rikkunshito, Induces HSP60 and Enhances Cytoprotection of Small Intestinal Mucosal Cells as a Nontoxic Chaperone Inducer

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Kumiko Tamaki ◽  
Michiro Otaka ◽  
Tomoyoshi Shibuya ◽  
Naoto Sakamoto ◽  
Soh Yamamoto ◽  
...  
Keyword(s):  
Herbal Medicine ◽  
Immunoblot Analysis ◽  
Intestinal Cell ◽  
Traditional Herbal Medicine ◽  
Intestinal Cell Line ◽  
Mucosal Cells ◽  
Hsp60 Expression ◽  
Small Intestinal ◽  
Intestinal Ulcers

Increasing incidence of small intestinal ulcers associated with nonsteroidal anti-inflammatory drugs (NSAIDs) has become a topic with recent advances of endoscopic technology. However, the pathogenesis and therapy are not fully understood. The aim of this study is to examine the effect of Rikkunshito (TJ-43), a traditional herbal medicine, on expression of HSP60 and cytoprotective ability in small intestinal cell line (IEC-6). Effect of TJ-43 on HSP60 expression in IEC-6 cells was evaluated by immunoblot analysis. The effect of TJ-43 on cytoprotective abilities of IEC-6 cells against hydrogen peroxide or indomethacin was studied by MTT assay, LDH-release assay, caspase-8 activity, and TUNEL. HSP60 was significantly induced by TJ-43. Cell necrosis and apoptosis were significantly suppressed in IEC-6 cells pretreated by TJ-43 with overexpression of HSP60. Our results suggested that HSP60 induced by TJ-43 might play an important role in protecting small intestinal epithelial cells from apoptosis and necrosis in vitro.

Is there a difference in the affinity of histamine H1 receptor antagonists for CNS and peripheral receptors? An in vitro study

1993 ◽  
Vol 232 (2-3) ◽  
pp. 199-205 ◽  
Author(s):  
Anton M. Ter Laak ◽  
Gabriëlle M. Donné-Op den Kelder ◽  
Aalt Bast ◽  
Hendrik Timmerman
Keyword(s):  
In Vitro Study ◽  
Vitro Study ◽  
H1 Receptor ◽  
Receptor Antagonists ◽  
Histamine H1 Receptor
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