scholarly journals Effects of In Ovo Supplementation with Nanonutrition (L-Arginine Conjugated with Ag NPs) on Muscle Growth, Immune Response and Heat Shock Proteins at Different Chicken Embryonic Development Stages

Animals ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 564 ◽  
Author(s):  
Sivakumar Allur Subramaniyan ◽  
Darae Kang ◽  
Sharif Hasan Siddiqui ◽  
Jinryong Park ◽  
Weishun Tian ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Broiler Chickens ◽  
Muscle Development ◽  
Muscle Growth ◽  
Hatching Rate ◽  
Ag Nps ◽  
In Ovo ◽  
Shock Proteins ◽  
Serum Glutamate

The aim of the study was to analyze the in ovo injection of inorganic and organic synthesized silver nanoparticles (Ag NPs) using Brassica oleracea L. var. capitate F. rubra (BOL) conjugation with L-Arginine (L-Arg) on the immune, muscle growth, survivability and hatchability of broiler chickens. The conjugation of L-Arg (100 μg) with 1000 µg of Ag NPs synthesized by (BOL)-extract and L-Arg (100 μg) conjugated with 100 µg of Ag NPs inorganic synthesized were injected into fertile eggs at 8 d, 14 d and 18 d of incubation. Survival and hatching rate were significantly improved in the dose of L-Arg (100 μg) with 1000 µg (BOL-Ag NPs) and L-Arg (100 μg) with 100 µg (C-Ag NPs) on 14 d injection whereas it was decreased on 8 d or 18 d injection. Moreover, the protein expression of muscle development markers such as myogenin and myoD were significantly uprelated in 14 d of incubation whereas the heat shock proteins (HSPs), such as HSP-60 and HSP-70, were significantly upregulated in 18 d incubation. In addition, the liver function marker of serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) were significantly decreased and the immunoglobulin (IgM) levels were increased in a 14 d incubation period in serum at the same concentration.

scholarly journals Effects of in ovo Supplementation with Nano-nutrition (L-Arginine Conjugated with Ag NPs) on Muscle Growth, Immune Response and Heat Shock Proteins at Different Chicken Embryonic Development Stages

2020 ◽  
Author(s):  
Sivakumar Subramaniyan ◽  
Kang Darae ◽  
Sharif Hasan Siddiqui ◽  
Jin Ryong Park ◽  
Weishun Tian ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Muscle Development ◽  
Muscle Growth ◽  
Nano Particles ◽  
Ag Nps ◽  
In Ovo ◽  
In Ovo Injection ◽  
Shock Proteins ◽  
Serum Glutamate

The aim of the study was to analyze the in ovo injection of chemically and biologically synthesized silver nano-particles (Ag NPs) using Brassica oleracea L. var capitate f. Rubra, (BOL) conjugation with L-Arginine (L-Arg) on the immune, muscle growth, survivability and hatchability of the broiler chickens. L-Arg (100 μg) conjugated with 1000 µg of Ag NPs synthesized by (BOL)-extract and L-Arg (100 μg) conjugated with 100 µg of Ag NPs chemically synthesized were injected into fertile eggs at 8d, 14d and 18d of incubation. Living embryo and hatched chicks were calculated. Survivability and hatchability were not affected by the injected dose of L-Arg (100 μg) with 1000 µg (BOL-Ag NPs) and L-Arg (100 μg) with 100 µg (C-Ag NPs) but it significantly improved when the eggs were injected on day 14 of incubation compared with those injected on days 8 or 18. Moreover, the protein expression of muscle development markers such as myogenin and myoD were significantly up-related in 14 d of incubation whereas the heat shock proteins (HSPs) such as HSP-60 and HSP-70 were significantly up-regulated in 18 d incubation. In addition, the in ovo injection on 18 d significantly increased the serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) as well the immunoglobulin (IgM) levels were increased in 14d incubation period in serum at the same concentration.

scholarly journals Nano-Nutrition to Chicken at Different Embryonic Development Stages: Using Conjugated L-Arginine with Ag NPs to Improve Muscle Growth and Immune Related Proteins

2019 ◽  
Author(s):  
Sivakumar Subramaniyan ◽  
Kang Da Rae ◽  
Sharif Hasan Siddiqui ◽  
Jin Ryong Park ◽  
Weishun Tian ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Muscle Development ◽  
Muscle Growth ◽  
Nano Particles ◽  
Ag Nps ◽  
In Ovo ◽  
In Ovo Injection ◽  
Shock Proteins ◽  
Related Proteins ◽  
Serum Glutamate

The aim of the study was to analyze the in ovo injection of chemically and biologically synthesized silver nano-particles (Ag NPs) using Brassica oleracea L. var capitate f. Rubra, (BOL) conjugation with L-Arginine (L-Arg) on the immune, muscle growth, survivability and hatchability of the broiler chickens. L-Arg (100 μg) with 1000 µg (BOL-Ag NPs) and L-Arg (100 μg) with 100 µg (C-Ag NPs) were injected into fertile eggs at 8, 14 and 18 of incubation. Survival and hatched chicks were calculated. Survivability and hatchability were unaffected by the injected dose of L-Arg (100 μg) with 1000 µg (BOL-Ag NPs) and L-Arg (100 μg) with 100 µg (C-Ag NPs) but it significantly improved when the eggs were injected on day 14 of incubation compared with those injected on days 8 or 18. Moreover, the protein expression of muscle development markers such as myogenin and myoD were significantly up-related in 14 d incubation whereas the heat shock proteins (HSPs) such as HSP-60 and HSP-70 were significantly up-regulated in 18 d incubation. In addition, the in ovo injection on 18 d significantly increased the serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT) as well the immunoglobulin (IgM) levels were increased in 14-day incubation period in serum at the same concentration. Collectively, these results indicate that injecting L-Arg (100 μg) with 1000 µg of BOL-Ag NPs or L-Arg (100 μg) with 100 µg of C-Ag NPs to eggs at day 14 after incubation could improve their hatchability, survival rate, and muscle growth marker.

scholarly journals Effect of In Ovo Injection of L-Arginine in Different Chicken Embryonic Development Stages on Post-Hatchability, Immune Response, and Myo-D and Myogenin Proteins

Animals ◽  
2019 ◽  
Vol 9 (6) ◽  
pp. 357 ◽  
Author(s):  
Sivakumar Allur Subramaniyan ◽  
Da Rae Kang ◽  
Jin Ryong Park ◽  
Sharif Hasan Siddiqui ◽  
Palanisamy Ravichandiran ◽  
...  
Keyword(s):  
Immune Response ◽  
Body Weight ◽  
Developmental Stages ◽  
Muscle Growth ◽  
In Ovo ◽  
Serum Glutamate Pyruvate Transaminase ◽  
In Ovo Injection ◽  
Shock Proteins ◽  
Related Proteins ◽  
Serum Glutamate

The aim of this study was to evaluate the effect of in ovo injection with different ratios of L-arginine (L-Arg) into Ross broiler eggs at three different embryonic developmental stages (eighth day (d), 14th day, and 18th day) on the survival, hatchability, and body weight (BW) of one-day-old hatched chicks. Additionally, we have analyzed the levels of serum glutamate oxaloacetate transaminase (SGOT) and serum glutamate pyruvate transaminase (SGPT), the protein expression of heat shock proteins (HSPs), and we have also determined micronuclei (MN) and nuclear abnormality (NA). In addition, the genotoxic effect was observed in peripheral blood cells such as the presence of micronuclei and nuclear abnormalities in the experimental groups. The results showed that survival and hatching rates as well as body weight were increased on the 14th day of incubation compared to the eighth and 18th day of incubation at lower concentrations of L-Arg. Moreover, the levels of SGOT and SGPT were also significantly (p < 0.05) increased on the 14th day of incubation at the same concentration (100 μg/μL/egg) of injection. In addition, immunoglobulin (IgM) levels were increased on the 14th day of incubation compared to other days. The protein expressions of HSP-47, HSP-60, and HSP-70 in the liver were significantly down-regulated, whereas the expression of myogenin and myoblast determination protein (MyoD) were significantly up-regulated on the 14th day after incubation when treated with all different doses such as 100 μg, 1000 μg, and 2500 μg/μL/egg, namely 3T1, 3T2, and 3T3, respectively. However, the treatment with low doses of L-Arg down-regulated the expression levels of those proteins on the 14th day of incubation. Histopathology of the liver by hematoxylin and eosin (H&E) staining showed that the majority of liver damage, specifically intracytoplasmic vacuoles, were observed in the 3T1, 3T2, and 3T3 groups. The minimum dose of 100 μg/mL/egg on the 14th day of incubation significantly prevented intracytoplasmic vacuole damages. These results demonstrate that in ovo administration of L-Arg at (100 μg/μL/egg) may be an effective method to increase chick BW, hatch rate, muscle growth-related proteins, and promote the immune response through increasing IgM on the 14th day of the incubation period.

scholarly journals Contribution of small heat shock proteins to muscle development and function

FEBS Letters ◽  
2014 ◽  
Vol 588 (4) ◽  
pp. 517-530 ◽  
Author(s):  
Magda Dubińska-Magiera ◽  
Jadwiga Jabłońska ◽  
Jolanta Saczko ◽  
Julita Kulbacka ◽  
Teresa Jagla ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Proteins ◽  
Muscle Development ◽  
Small Heat Shock Proteins ◽  
Shock Proteins ◽  
And Function

scholarly journals Effect of in ovo Injection of L-Arginine in Different Chicken Embryonic Development Stages on Post-hatch Growth Performance, Immune Response and Muscle Development Proteins

2019 ◽  
Author(s):  
Sivakumar Allur Subramaniyan ◽  
Da Rae Kang ◽  
Jin Ryong Park ◽  
Sharif Hasan siddiqui ◽  
Palanisamy Ravichandiran ◽  
...  
Keyword(s):  
Immune Response ◽  
Body Weight ◽  
Muscle Development ◽  
Developmental Stages ◽  
Muscle Growth ◽  
In Ovo ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
In Ovo Injection ◽  
Shock Proteins ◽  
Related Proteins

The objective of this study was to evaluate the effect of in ovo injection of L-arginine (L-Arg) into Ross broiler eggs at different embryonic developmental stages on their survival, hatchability, and body weight (BW). Additionally, we have analyzed the levels of serum glutamic-oxaloacetic transaminase (SGOT) and serum glutamic-pyruvic transaminase (SGPT), protein expression of heat shock proteins (HSPs), also we have the determined micronuclei (MN) and nuclear abnormality (NA). Results showed that survival and hatching rates as well as body weight were increased on the 14th day incubation compared to 8th and 18th day incubation at lower concentration of L-Arg. Moreover, the levels of SGOT and SGPT were also significantly (P &lt; 0.05) increased at 14th day incubation at the same concentration (100&mu;g/&mu;l/egg) of injection. In addition, IgM levels were increased on the 14th day incubation compared to other days. The protein expressions of HSP-47, HSP-60, and HSP-70 in the liver were significantly down-regulated whereas the expression of myogenin and MyoD were significantly up-regulated on the 14th day after incubation in treated with all different doses such as 100&mu;g, 1000&mu;g and 2500&mu;g/&mu;l/egg namely 3T1, 3T2 and 3T3 respectively. However, the treatment with low dose of L-Arg down-regulated expression levels of those proteins on the 14th day incubation. Histopathology of liver by hematoxylin and eosin (H&amp;E) straining showed that the majority of liver damage, specifically intracytoplasmic vacuoles, were observed in 3T1, 3T2, and 3T3. The minimum dose of 100 &mu;g/ml/egg on the 14th day of incubation significantly prevented intracytoplasmic vacuole damages. These results demonstrate that in ovo administration of L-Arg at (100&mu;g/&mu;l/egg) may be an effective method to increase chick BW, hatch rate, increasing muscle growth related proteins and promote the immune response through increasing IgM on the 14th day of incubation period.

scholarly journals Effect of in ovo Injection of L-Arginine in Different Chicken Embryonic Development Stages on Post-Hatchability, Immune Response and Muscle Development Proteins

2019 ◽  
Author(s):  
Sivakumar Subramaniyan ◽  
Da Rae Kang ◽  
Jin Ryong Park ◽  
Sharif Hasan siddiqui ◽  
Palanisamy Ravichandiran ◽  
...  
Keyword(s):  
Immune Response ◽  
Body Weight ◽  
Muscle Development ◽  
Developmental Stages ◽  
Muscle Growth ◽  
In Ovo ◽  
Serum Glutamic Oxaloacetic Transaminase ◽  
Hematoxylin And Eosin ◽  
Shock Proteins ◽  
Related Proteins

The objective of this study was to evaluate the effect of in ovo injection of L-arginine (L-Arg) into Ross broiler eggs at different embryonic developmental stages on their survival, hatchability, and body weight (BW). Additionally, we have analyzed the levels of serum glutamic-oxaloacetic transaminase (SGOT) and serum glutamic-pyruvic transaminase (SGPT), protein expression of heat shock proteins (HSPs), also we have the determined micronuclei (MN) and nuclear abnormality (NA). Results showed that survival and hatching rates as well as body weight were increased on the 14th day incubation compared to 8th and 18th day incubation at lower concentration of L-Arg. Moreover, the levels of SGOT and SGPT were also significantly (P &lt; 0.05) increased at 14th day incubation at the same concentration (100&mu;g/&mu;l/egg) of injection. In addition, IgM levels were increased on the 14th day incubation compared to other days. The protein expressions of HSP-47, HSP-60, and HSP-70 in the liver were significantly down-regulated whereas the expression of myogenin and MyoD were significantly up-regulated on the 14th day after incubation in treated with all different doses such as 100&mu;g, 1000&mu;g and 2500&mu;g/&mu;l/egg namely 3T1, 3T2 and 3T3 respectively. However, the treatment with low dose of L-Arg down-regulated expression levels of those proteins on the 14th day incubation. Histopathology of liver by hematoxylin and eosin (H&amp;E) straining showed that the majority of liver damage, specifically intracytoplasmic vacuoles, were observed in 3T1, 3T2, and 3T3. The minimum dose of 100 &mu;g/ml/egg on the 14th day of incubation significantly prevented intracytoplasmic vacuole damages. These results demonstrate that in ovo administration of L-Arg at (100&mu;g/&mu;l/egg) may be an effective method to increase chick BW, hatch rate, increasing muscle growth related proteins and promote the immune response through increasing IgM on the 14th day of incubation period.

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