scholarly journals CircAgtpbp1 Acts as a Molecular Sponge of miR-543-5p to Regulate the Secretion of GH in Rat Pituitary Cells

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 558
Author(s):  
ZeWen Yu ◽  
WenZhi Ren ◽  
Tian Wang ◽  
WeiDi Zhang ◽  
ChangJiang Wang ◽  
...  
Keyword(s):  
Pituitary Gland ◽  
Inhibitory Effect ◽  
Pituitary Cells ◽  
Sequencing Analysis ◽  
Hormone Regulation ◽  
Gh Secretion ◽  
Qrt Pcr ◽  
Rat Pituitary ◽  
Rat Pituitary Cells

CircRNAs have been identified to be expressed differently and stably in numerous species and tissues, but their functions in growth hormone (GH) secretion are still largely unknown. In summary, we have revealed a circRNA-miRNA-mRNA network that may play a biological role in the rat pituitary gland. First, we verified the chromosome location information of circAgtpbp1 according to sequencing analysis. The circAgtpbp1 characteristics were authenticated through PCR, qRT–PCR, treating with RNase and fluorescent in situ hybridization (FISH). Second, we detected the expression pattern of circAgtpbp1 in the rat anterior pituitary by qRT–PCR. We also designed circAgtpbp1 siRNA and constructed overexpression plasmid to evaluate the effect of circAgtpbp1 function on GH secretion by qRT–PCR, ELISA and Western blot. CircAgtpbp1 is a stable, truly circular molecule. We found that circAgtpbp1 interacted with miR-543-5p and can regulate GH secretion in pituitary cells through a circAgtpbp1-miR-543-5p-GH axis. Overall, the evidence generated by our study suggests that circAgtpbp1 can act as a sponge of miR-543-5p to reduce the inhibitory effect of miR-543-5p on Gh1 and further promote GH secretion. These findings expand our existing knowledge on the mechanisms of hormone regulation in the pituitary gland.

Inhibition by extracellular Na+ replacement of GRF-induced GH secretion from rat pituitary cells

1988 ◽  
Vol 254 (4) ◽  
pp. E476-E481 ◽  
Author(s):  
M. Kato ◽  
M. A. Hattori ◽  
M. Suzuki
Keyword(s):  
Anterior Pituitary ◽  
Channel Blocker ◽  
Na Channel ◽  
Pituitary Cells ◽  
Ionic Mechanism ◽  
Camp Production ◽  
Normal Medium ◽  
Gh Secretion ◽  
Rat Pituitary ◽  
Rat Pituitary Cells

To further clarify the ionic mechanism of the action of growth hormone (GH)-releasing factor (hGRF) on GH secretion, the involvement of extracellular Na+ was studied in perifused dispersed rat anterior pituitary cells. Replacing extracellular Na+ with mannitol or tris(hydroxymethyl)aminomethane (Tris+) suppressed hGRF- and dibutyryl adenosine 3',5'-cyclic monophosphate (DBcAMP)-induced GH secretion. The peak responses to a 2-min application of 1 nM hGRF were 165.0 +/- 5.6 ng/ml (normal medium, mean +/- SE), 21.2 +/- 1.4 ng/ml (Na+-free, mannitol medium), and 18.0 +/- 1.7 ng/ml (Na+-free, Tris+ medium). GH secretion induced by DBcAMP was also suppressed by Na+ replacement to less than 50% of that in normal medium. However, either 15 or 30 mM KCl-stimulated GH secretion was not markedly affected by replacement of Na+ with either compound. Tetrodotoxin, a voltage-sensitive Na+ channel blocker, had no effect on either hGRF- or excess K+-induced GH secretion. cAMP production by hGRF was not greatly affected by replacing extracellular Na+. Thus extracellular Na+ plays an important role in hGRF-induced GH secretion, especially in the process after cAMP production. The involvement of cAMP-sensitive Na+ channels in hGRF-stimulated GH secretion is discussed.

Studies on the Conditions Determining the Inhibitory Effect of Somatostatin on Adrenocorticotropin, Prolactin and Thyrotropin Release by Cultured Rat Pituitary Cells

1989 ◽  
Vol 50 (1) ◽  
pp. 44-50 ◽  
Author(s):  
Steven W. Lamberts ◽  
Joke Zuyderwijk ◽  
Fred den Holder ◽  
Peter van Koetsveld ◽  
Leo Hofland
Keyword(s):  
Inhibitory Effect ◽  
Pituitary Cells ◽  
Rat Pituitary ◽  
Rat Pituitary Cells

Dichotomic action of glucocorticoids on growth hormone secretion

1987 ◽  
Vol 116 (2) ◽  
pp. 165-171 ◽  
Author(s):  
Koji Nakagawa ◽  
Tatsuya Ishizuka ◽  
Takao Obara ◽  
Miyao Matsubara ◽  
Kazumasa Akikawa
Keyword(s):  
Hormone Secretion ◽  
Growth Hormone Secretion ◽  
Inhibitory Effect ◽  
Female Rats ◽  
Pituitary Cells ◽  
Gh Secretion ◽  
Normal Rat ◽  
Rat Pituitary Cells

Abstract. The mechanism of apparently discrepant actions of glucocorticoids (GC) on GH secretion, in vivo suppression and in vitro potentiation, was studied in rats. Dexamethasone (Dex), at the concentration of 50 nmol/l, Potentiated basal and GHRH-stimulated GH release from monolayer culture of normal rat pituitary cells in 48 h. On the other hand, in vivo administration of Dex, 165 μg daily for 3 days, consistently suppressed serum GH levels in female rats. In these rats, the hypothalamic content of immunoreactive (IR) SRIH was significantly increased, whereas that of IR-GHRH was significantly decreased in comparison with the untreated rats. Bioassayable GH-releasing activity was also lower in Dex-treated rats. These findings indicate that the suppressing effect of GC on GH release in vivo is, at least partially, due to the increase in hypothalamic SRIH release and probably also to the decrease in GHRH release, and these effects surpass the potentiating effect of GC on GH release at the pituitary level, resulting in a net inhibitory effect in vivo.

PACAP 38 regulates prolactin (PRL) and growth hormone (GH) secretion and gene expression in cultured rat pituitary cells

1992 ◽  
Vol 40 (2) ◽  
pp. 289
Author(s):  
L. Zheng ◽  
M. Kazemzadeh ◽  
B. Velkeniers ◽  
A. Vandermeers ◽  
J. Christophe ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Hormone ◽  
Pituitary Cells ◽  
Gh Secretion ◽  
Rat Pituitary ◽  
Rat Pituitary Cells

GROWTH IN MONOLAYER CULTURE OF RAT PITUITARY CELLS WHICH SYNTHETIZE AND RELEASE ACTH

1975 ◽  
Vol 79 (3) ◽  
pp. 421-430 ◽  
Author(s):  
R. E. Lang ◽  
I. Hilwig ◽  
K. H. Voigt ◽  
H. L. Fehm ◽  
E. F. Pfeiffer
Keyword(s):  
Pituitary Gland ◽  
Monolayer Culture ◽  
Pituitary Cells ◽  
Dependent Manner ◽  
Antibody Technique ◽  
Gland Cells ◽  
Rat Pituitary ◽  
Rat Pituitary Cells ◽  
Dose Dependent ◽  
Acth Release

ABSTRACT Cultures of rat pituitary gland cells were developed to study biosynthesis and release of ACTH. ACTH measurement was accomplished by radioimmunoassay. ACTH release was observed following stimulation with theophylline and cAMP in a dose-dependent manner. Biosynthesis was demonstrated by incorporation of 3H-phenylalanine into the hormone, employing a double antibody technique.

Inhibitory effect of the uterus on plasma and pituitary FSH in rats

1990 ◽  
Vol 124 (2) ◽  
pp. 183-189 ◽  
Author(s):  
J. C. Biro ◽  
P. Eneroth
Keyword(s):  
Molecular Weight ◽  
Chromatographic Analysis ◽  
Plasma Concentrations ◽  
Inhibitory Effect ◽  
Inhibitory Response ◽  
Pituitary Cells ◽  
Pregnant Rats ◽  
Rat Pituitary ◽  
Rat Pituitary Cells

ABSTRACT Plasma concentrations of FSH and LH were measured in ovariectomized, ovohysterectomized, hysterectomized and sham-operated adult, non-pregnant rats at 3, 14, 21 and 28 days after operation. From day 21 after the operation onwards, there were higher concentrations of FSH in plasma in ovohysterectomized than in ovariectomized animals. The concentration of LH was not influenced by hysterectomy. The inhibitory response of FSH and LH to a single dose of oestradiol was not altered by any of the operations. By 2 weeks after surgery, pituitary FSH content had increased in ovohysterectomized animals compared with ovariectomized ones, but this difference was eliminated when ovohysterectomized animals were treated with crude uterine extract. Pituitary contents of LH and prolactin were not influenced by hysterectomy or by treatment with uterine extract, thus indicating the specificity of an inhibitory effect of the uterus on FSH levels. Treatment of hysterectomized and intact animals with uterine extract resulted in a reduction in the weight of the ovaries of 23–38% (P<0·05), indirectly showing the presence of an FSH-inhibiting substance in the extract. Fractionated uterine extract inhibited FSH synthesis by rat pituitary cells in vitro, but had no effect on LH synthesis. Chromatographic analysis indicated that the FSH-inhibiting substance in the uterus has a molecular weight of 10 000–20 000. Journal of Endocrinology (1990) 124, 183–189

scholarly journals Is GHRH Receptor Essential to GHRP-2-Induced GH Secretion in Primary Cultured Rat Pituitary Cells?

Endocrinology ◽  
2002 ◽  
Vol 143 (5) ◽  
pp. 1964-1967 ◽  
Author(s):  
Sang-Gun Roh ◽  
Chen Chen ◽  
Ki-Choon Choi ◽  
Yogendra Shrestha ◽  
Shin-Ichi Sasaki
Keyword(s):  
Pituitary Cells ◽  
Gh Secretion ◽  
Ghrh Receptor ◽  
Rat Pituitary ◽  
Rat Pituitary Cells

Plasma clearance, tissue uptake and expression of pituitary peptide 23/pancreatitis-associated protein in the rat

1995 ◽  
Vol 145 (3) ◽  
pp. 461-469 ◽  
Author(s):  
C Chakraborty ◽  
S Sharma ◽  
N Katsumata ◽  
L J Murphy ◽  
I C Schroedter ◽  
...  
Keyword(s):  
Male Rats ◽  
Pituitary Cells ◽  
Tissue Uptake ◽  
Recombinant Peptide ◽  
Tissue Extracts ◽  
Rat Pituitary ◽  
Rat Pituitary Cells ◽  
Plasma Half Life ◽  
Pancreatitis Associated Protein

Abstract The secretion of peptide 23 by rat pituitary cells is stimulated by growth hormone-releasing hormone and inhibited by somatostatin. Recent cloning of the cognate cDNA for peptide 23 revealed that it is identical to pancreatitis-associated protein (PAP). In the present study, the clearance and tissue uptake of recombinant peptide 23/PAP in normal adult male rats was assessed. The plasma half-life of recombinant peptide 23/PAP was 4·8 ±1·4 (s.d.) min. Maximal accumulation of radiolabelled peptide 23/PAP was observed in the kidney, stomach, small intestine and pancreas whereas negligible uptake was seen in the liver, lung or heart. Peptide 23/PAP was detected in a variety of tissue extracts using a radioimmunoassay. Extracts of ileum contained the highest concentrations of peptide 23/PAP. In situ hybridization analysis showed that peptide 23/PAP mRNA was highly expressed in the columnar epithelial cells of ileum, jejunum and duodenum. These observations demonstrate that peptide 23/PAP, a protein previously thought to be of pituitary origin, is widely expressed in the gastrointestinal tract and that it is rapidly removed from the circulation by the kidney and by tissues which express peptide 23/PAP. Journal of Endocrinology (1995) 145, 461–469

scholarly journals GH secretion by cultured rat pituitary cells under radiotherapy

1981 ◽  
Vol 15 (12) ◽  
pp. 1570-1570
Author(s):  
Z Hochberg ◽  
A Kuten ◽  
P Hertz ◽  
A Benderli
Keyword(s):  
Pituitary Cells ◽  
Gh Secretion ◽  
Rat Pituitary ◽  
Rat Pituitary Cells
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