Determination of Prolactin in Canine Saliva: Is it Possible to Use a Commercial ELISA kit?

Prolactin has been reported to be a remarkable index of stress response, both acute and chronic, in several species. The use of biological matrixes other than blood is receiving increasing interest in the study of hormones, due to the lower invasiveness in collection. This research aimed to investigate the possibility of using a commercial ELISA (enzyme-linked immunosorbent assay) kit for measuring canine prolactin in blood for the quantification of canine prolactin in saliva. Study 1 consisted of a validation protocol, using saliva samples collected from lactating and non-lactating dogs. Study 2 was conducted to investigate a possible correlation between prolactin concentration in saliva and plasma in sheltered dogs by using the same kit. Prolactin values were reliably read only when they came from blood samples, not from saliva, but tended to be low in most of the cases. Study 1 showed that saliva had a matrix effect. In study 2, saliva prolactin levels were low and in 42.9% of cases, not readable. No correlation between prolactin values in plasma and saliva was found (ρ=0.482; p=0.274). These findings suggested that the determination of prolactin in dog saliva through an ELISA kit created for measuring prolactin in dog blood was unreliable.

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EVALUATION OF A RECENTLY DEVELOPPED ENZYME LINKED IMMUNOSORBENT ASSAY FOR THE DETERMINATION OF ANTI-PHOSPHOLIPASE A2 RECEPTOR ANTIBODIES

10.26226/morressier.56e174d9d462b8028d88aca7 ◽

2016 ◽

Author(s):

Anne-Sophie Messiaen

Keyword(s):

Phospholipase A2 ◽

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay ◽

Phospholipase A2 Receptor ◽

Receptor Antibodies

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Biotin-avidin Mediated Competitive Enzyme-linked Immunosorbent Assay for Determination of Ketamine

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY (CHINESE VERSION) ◽

10.3724/sp.j.1096.2010.00117 ◽

2010 ◽

Vol 38 (1) ◽

pp. 117-120

Author(s):

Wen-Di ZHANG ◽

Ping SU ◽

Yi YANG ◽

Zhen-Quan GUO

Keyword(s):

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay

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Can Osteopontin Be Considered a Biomarker for Endometriosis?

Revista de Chimie ◽

10.37358/rc.17.9.5840 ◽

2017 ◽

Vol 68 (9) ◽

pp. 2132-2134

Author(s):

Daniela Roxana Albu (Matasariu) ◽

Elena Mihalceanu ◽

Alina Pangal ◽

Carmen Vulpoi ◽

Mircea Onofriescu ◽

...

Keyword(s):

Serum Level ◽

Immunosorbent Assay ◽

Pelvic Pain ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Multifactorial Disease ◽

Elisa Kit ◽

Healthy Women ◽

Progesterone Treatment ◽

Large Dispersion

Endometriosis is a multifactorial disease that is manifested by infertility and pelvic pain. The purpose of the study was to evaluate the effect of progesterone treatment on the serum level of osteopontin, a multipotent cytokine, in patients with endometriosis. The study was prospective and we evaluated osteopontin levels that were measured in the serum of 40 patients with endometriosis and 12 healthy women using a standardized Enzyme-Linked Immunosorbent Assay (ELISA) kit. Osteopontin seric levels were lower in endometriosis patients and increased after progesterone treatment. Because of the large dispersion of data even in the control group, we find the association between osteopontin and endometriosis questionable.

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SENSITIVE AND SPECIFIC ENZYME-LINKED IMMUNOSORBENT ASSAY FOR UROKINASE-TYPE PLASMINOGEN ACTIVATOR IN HUMAN PLASMA

10.1055/s-0038-1644425 ◽

1987 ◽

Cited By ~ 1

Author(s):

J Grøndahl-HANSEN ◽

N Agerlin ◽

L S Nielsen ◽

K Danø

Keyword(s):

Plasminogen Activator ◽

Immunosorbent Assay ◽

Enzyme Linked Immunosorbent Assay ◽

Mean Values ◽

Amino Terminal ◽

Type Plasminogen Activator ◽

Healthy Donors ◽

Urokinase Type Plasminogen Activator ◽

The Mean

An enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of human urokinase-type plasminogen activator (u-PA) in plasma and serum. Microtiter plates were coated with a monoclonal antibody and incubated with standard or sample. Bound u-PA was quantitated with polyclonal antibodies conjugated with biotin, followed by avidin-peroxidase. The assay was 10-fold as sensitive as other previously reported ELISAs, the detection limit being approximately 1 pg of u-PA in a volume of 100 μl with a linear dose-response up to 15 pg of u-PA. The assay detected active u-PA and its inactive proenzyme form equally well and the recovery of both forms was higher than 90% in plasma. A variety of structurally related proteins, including t-PA, were tested, but no reaction with proteins other than u-PA and its amino-terminal degradation product were observed. The intra-assay and inter-assay coefficients of variation for determination of u-PA in plasma were 7.6% and 8.4%, respectively. The assay was equally applicable to serum. The values obtained with plasma and serum were similar, and the results were not affected by small variations in the preparation of the samples. The ELISA was used to measure the concentration of u-PA in plasma from 34 healthy donors. The mean values for u-PA in plasma from healthy donors was 1.1 ng/ml ± 0.3 ng/ml (SD) (range 0.6 - 1.5 ng/ml). No significant differences were found between men and women and no correlation between u-PA concentration and age could be demonstrated.The mean u-PA concentration in plasma from healthy donors obtained in this study is substantially lower than that reported by others. This might be due to different methods of determination of the protein content of the standard preparations or to differences in the specificity of the assays.

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