Prevalence, Genetic Diversity, Antimicrobial Resistance, and Toxigenic Profile of Vibrio vulnificus Isolated from Aquatic Environments in Taiwan

Vibrio vulnificus is a gram-negative, opportunistic human pathogen associated with life-threatening wound infections and is commonly found in warm coastal marine water environments, globally. In this study, two fishing harbors and three tributaries of the river basin were analyzed for the prevalence of V. vulnificus in the water bodies and shellfish that are under the pressure of external pollutions. The average detection rate of V. vulnificus in the river basins and fishing harbors was 8.3% and 4.2%, respectively, in all seasons. A total of nine strains of V. vulnificus were isolated in pure cultures from 160 samples belonging to river basins and fishing harbors to analyze the antibiotic susceptibility, virulence gene profiles, and enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) fingerprinting. All isolates were susceptible to 10 tested antibiotics. The genotypic characterization revealed that 11.1% (n = 1/9) strain was nonvirulent, whereas 88.9% (n = 8/9) isolates were virulent strains, which possessed the four most prevalent toxin genes such as vcgC (88.9%), 16S B (88.9%), vvhA (88.9%), and manIIA (88.9%), followed by nanA (77.8%), CPS1 (66.7), and PRXII (44.4%). Additionally, ERIC-PCR fingerprinting grouped these nine isolates into two main clusters, among which the river basin isolates showed genetically diverse profiles, suggesting multiple sources of V. vulnificus. Ultimately, this study highlighted the virulent strains of V. vulnificus in the coastal aquatic environments of Taiwan, harboring a potential risk of infection to human health through water-borne transmission.

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Real-Time PCR Analysis of Vibrio vulnificus from Oysters

Applied and Environmental Microbiology ◽

10.1128/aem.69.12.7137-7144.2003 ◽

2003 ◽

Vol 69 (12) ◽

pp. 7137-7144 ◽

Cited By ~ 99

Author(s):

Mark S. Campbell ◽

Anita C. Wright

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Vibrio Vulnificus ◽

Pcr Analysis ◽

Viability Assay ◽

Nonculturable Cells ◽

Pure Cultures ◽

Plate Counts ◽

Opportunistic Human Pathogen ◽

Species Specific

ABSTRACT Vibrio vulnificus is an opportunistic human pathogen commonly found in estuarine environments. Infections are associated with raw oyster consumption and can produce rapidly fatal septicemia in susceptible individuals. Standard enumeration of this organism in shellfish or seawater is laborious and inaccurate; therefore, more efficient assays are needed. An oligonucleotide probe derived from the cytolysin gene, vvhA, was previously used for colony hybridizations to enumerate V. vulnificus. However, this method requires overnight growth, and vibrios may lack culturability under certain conditions. In the present study, we targeted the same locus for development of a TaqMan real-time PCR assay. Probe specificity was confirmed by amplification of 28 V. vulnificus templates and by the lack of a PCR product with 22 non-V. vulnificus strains. Detection of V. vulnificus in pure cultures was observed over a 6-log-unit linear range of concentration (102 to 108 CFU ml−1), with a lower limit of 72 fg of genomic DNA μl of PCR mixture−1 or the equivalent of six cells. Similar sensitivity was observed in DNA extracted from mixtures of V. vulnificus and V. parahaemolyticus cells. Real-time PCR enumeration of artificially inoculated oyster homogenates correlated well with colony hybridization counts (r 2 = 0.97). Numbers of indigenous V. vulnificus cells in oysters by real-time PCR showed no significant differences from numbers from plate counts with probe (t test; P = 0.43). Viable but nonculturable cells were also enumerated by real-time PCR and confirmed by the BacLight viability assay. These data indicate that real-time PCR can provide sensitive species-specific detection and enumeration of V. vulnificus in seafood.

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Assessing Water Balance Closure Using Multiple Data Assimilation and Remote Sensing-Based Datasets for Canada

Journal of Hydrometeorology ◽

10.1175/jhm-d-20-0131.1 ◽

2021 ◽

Author(s):

Jefferson S. Wong ◽

Xuebin Zhang ◽

Shervan Gharari ◽

Rajesh R. Shrestha ◽

Howard S. Wheater ◽

...

Keyword(s):

Remote Sensing ◽

Data Assimilation ◽

Water Balance ◽

River Basin ◽

Land Surface ◽

Water Storage ◽

River Basins ◽

Multiple Sources ◽

Discharge Data ◽

Multiple Data

AbstractObtaining reliable water balance estimates remains a major challenge in Canada for large regions with scarce in situ measurements. Various remote sensing products can be used to complement observation-based datasets and provide an estimate of the water balance at river basin or regional scales. This study provides an assessment of the water balance using combinations of various remote sensing and data assimilation-based products and quantifies the non-closure errors for river basins across Canada, ranging from 90,900 to 1,679,100 km2, for the period from 2002 to 2015. A water balance equation combines the following to estimate the monthly water balance closure: multiple sources of data for each water budget component, including two precipitation products - the global product WATCH Forcing Data ERA-Interim (WFDEI), and the Canadian Precipitation Analysis (CaPA); two evapotranspiration products - MODIS, and Global Land-surface Evaporation: the Amsterdam Methodology (GLEAM); one source of water storage data - GRACE from three different centers; and observed discharge data from hydrometric stations (HYDAT). The non-closure error is attributed to the different data products using a constrained Kalman filter. Results show that the combination of CaPA, GLEAM, and the JPL mascon GRACE product tended to outperform other combinations across Canadian river basins. Overall, the error attributions of precipitation, evapotranspiration, water storage change, and runoff were 36.7, 33.2, 17.8, and 12.2 percent, which corresponded to 8.1, 7.9, 4.2, and 1.4 mm month-1, respectively. In particular, non-closure error from precipitation dominated in Western Canada, whereas that from evapotranspiration contributed most in the Mackenzie River basin.

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Identification of sibling species in the genus Eucyclops from water-bodies of Ukraine and Russia using crossbreeding studies

Ecology and Noospherology ◽

10.15421/031406 ◽

2014 ◽

Vol 25 (1-2) ◽

pp. 61-68 ◽

Cited By ~ 1

Author(s):

V. I. Monchenko ◽

L. P. Gaponova ◽

V. R. Alekseev

Keyword(s):

Cryptic Species ◽

River Basin ◽

Black Sea ◽

Species Complex ◽

River Basins ◽

Water Bodies ◽

Similar Species ◽

Basin Water ◽

Different Populations ◽

Baltic Sea Basin

Crossbreeding experiments were used to estimate cryptic species in water bodies of Ukraine and Russia because the most useful criterion in species independence is reproductive isolation. The problem of cryptic species in the genus Eucyclops was examined using interpopulation crosses of populations collected from Baltic Sea basin (pond of Strelka river basin) and Black Sea basin (water-reservoires of Dnieper, Dniester and Danube rivers basins). The results of reciprocal crosses in Eucyclops serrulatus-group are shown that E. serrulatus from different populations but from water bodies belonging to the same river basin crossed each others successfully. The interpopulation crosses of E. serrulatus populations collected from different river basins (Dnipro, Danube and Dniester river basins) were sterile. In this group of experiments we assigned evidence of sterility to four categories: 1) incomplete copulation or absence of copulation; 2) nonviable eggs; 3) absence of egg membranes or egg sacs 4) empty egg membranes. These crossbreeding studies suggest the presence of cryptic species in the E. serrulatus inhabiting ecologically different populations in many parts of its range. The same crossbreeding experiments were carries out between Eucyclops serrulatus and morphological similar species – Eucyclops macruroides from Baltic and Black Sea basins. The reciprocal crossings between these two species were sterile. Thus taxonomic heterogeneity among species of genus Eucyclops lower in E. macruroides than in E. serrulatus. The interpopulation crosses of E. macruroides populations collected from distant part of range were fertile. These crossbreeding studies suggest that E. macruroides species complex was evaluated as more stable than E. serrulatus species complex.

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