Chemical Composition and Protective Effect of Young Barley (Hordeum vulgare L.) Dietary Supplements Extracts on UV-Treated Human Skin Fibroblasts in In Vitro Studies

Young barley seems to be a promising material for use as nutricosmetic due to the presence of many biologically active compounds. The aim of this study was to evaluate the effect of Hordeum vulgare L. extracts on human skin fibroblasts exposed to ultraviolet radiation B (UVB) radiation. Analysis of the chemical composition showed a predominance of 9,12,15-octadecatrienoic acid. The quality assessment showed that young barley preparations have high total polyphenolic content (TPC) and favourable total antioxidant status (TAS). They also contain antioxidant elements such as zinc, copper, and selenium. Furthermore, the analyzed products were found to be safe in terms of toxic elements (lead, cadmium and mercury) and lack of cytotoxic effect of young barley extracts on cells. In vitro bioactivity assays showed that young barley extract increased the survival rate and accelerated the migration of fibroblasts in research models with UVB radiation. The application of both extracts caused an increase in DNA biosynthesis, and in the number of cells arrested in S phase. Moreover, an inhibitory effect of the tested extracts on the expression of matrix metalloproteinase 2 (MMP-2) and matrix metalloproteinase 9 (MMP-9) was observed. The results indicate that young barley extracts, due to protective as well as restorative effect, could potentially be used in the production of nutricosmetics and skin care products.

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Determination of in vitro photoprotection on human skin fibroblasts and keratinocytes after UVA and UVB radiation by aqueous and ethanolic extracts from Stellaria media herb

Planta Medica ◽

10.1055/s-0035-1565774 ◽

2015 ◽

Vol 81 (16) ◽

Author(s):

A Parzonko ◽

M Lenart ◽

A Bazylko

Keyword(s):

Human Skin ◽

Skin Fibroblasts ◽

Uvb Radiation ◽

Human Skin Fibroblasts ◽

Stellaria Media ◽

Ethanolic Extracts

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The Effect of Practolol, In Vitro Generated Metabolites of Practolol and Chemical Analogues on the Rate of Growth of Human Skin Fibroblasts

Alternatives to Laboratory Animals ◽

10.1177/026119298401200205 ◽

1984 ◽

Vol 12 (2) ◽

pp. 89-97

Author(s):

Graham R. Elliott ◽

H.E. Amos ◽

James W. Bridges

Keyword(s):

Human Skin ◽

Psoriasis Patient ◽

Skin Fibroblasts ◽

Human Skin Fibroblasts ◽

Cell Type ◽

Normal Human Skin ◽

Rate Of Growth ◽

Structural Analogues ◽

Normal Human

The rate of growth of normal human skin fibroblasts was inhibited in a dose related, reversible, fashion by practolol (N-4-(2-hydroxy)-3 (1-methyl)-aminopropoxyphenylacetamine) (ID50 1.35 ± 0.14 x 10-3M), propranolol (1-(isopropylamino)-3(1-naphthyl-oxy)-2-propranolol) (ID50 0.145 ± 0.02 x 10-3M) and paracetamol (N-(4-hydroxyphenyl) acetamide) (ID50 0.85 ± 0.2 x 10-3M). Skin fibroblasts isolated from a psoriasis patient were more sensitive towards practolol (ID50 0.48 ± 0.14 x 10-3M) and propranolol (ID50 0.032 ± 0.002 x 10-3M), but less sensitive towards paracetamol (ID50 1.3 ± 0.07 x 10-3M). In vitro generated metabolites of practolol, using normal or Arochlor 1254-pretreated hamster liver preparations, and structural analogues of practolol had no effect upon the growth of either cell type.

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4-Methylumbelliferone induces the expression of membrane type 1-matrix metalloproteinase in cultured human skin fibroblasts

Biochemical and Biophysical Research Communications ◽

10.1016/s0006-291x(02)02516-0 ◽

2002 ◽

Vol 298 (5) ◽

pp. 646-650 ◽

Cited By ~ 8

Author(s):

Toshiya Nakamura ◽

Takashi Ishikawa ◽

Naoki Nanashima ◽

Tomisato Miura ◽

Hiroyuki Nozaka ◽

...

Keyword(s):

Matrix Metalloproteinase ◽

Human Skin ◽

Skin Fibroblasts ◽

Human Skin Fibroblasts ◽

Membrane Type

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Expression of Basement Membrane Zone Genes Coding for Type IV Procollagen and Laminin by Human Skin Fibroblasts in Vitro: Elevated α1(IV) Collagen mRNA Levels in Lipoid Proteinosis

Journal of Investigative Dermatology ◽

10.1111/1523-1747.ep12560934 ◽

1988 ◽

Vol 90 (5) ◽

pp. 734-738 ◽

Cited By ~ 42

Author(s):

David R. Olsen ◽

Mon-Li. Chu ◽

Jouni. Uitto

Keyword(s):

Basement Membrane ◽

Human Skin ◽

Basement Membrane Zone ◽

Skin Fibroblasts ◽

Mrna Levels ◽

Human Skin Fibroblasts ◽

Collagen Mrna ◽

Type Iv ◽

Lipoid Proteinosis

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In vitro differentiation characteristics of human skin fibroblasts: correlations with radiotherapy-induced breast fibrosis in patients

International Journal of Radiation Biology ◽

10.1080/095530000138880 ◽

2000 ◽

Vol 76 (2) ◽

pp. 231-240 ◽

Cited By ~ 13

Author(s):

N. S. Russell, P. C. Lara, A. Grummels

Keyword(s):

Human Skin ◽

Skin Fibroblasts ◽

Human Skin Fibroblasts ◽

In Vitro Differentiation

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Basal Level of Autophagy Is Increased in Aging Human Skin Fibroblasts In Vitro, but Not in Old Skin

PLoS ONE ◽

10.1371/journal.pone.0126546 ◽

2015 ◽

Vol 10 (5) ◽

pp. e0126546 ◽

Cited By ~ 21

Author(s):

Dino Demirovic ◽

Carine Nizard ◽

Suresh I. S. Rattan

Keyword(s):

Human Skin ◽

Skin Fibroblasts ◽

Human Skin Fibroblasts

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Root extractive from Daphne genkwa benefits in wound healing of anal fistula through up-regulation of collagen genes in human skin fibroblasts

Bioscience Reports ◽

10.1042/bsr20170182 ◽

2017 ◽

Vol 37 (2) ◽

Cited By ~ 5

Author(s):

Dong Yang ◽

Jun-hua Xu ◽

Ren-jie Shi

Keyword(s):

Wound Healing ◽

Human Skin ◽

Anal Fistula ◽

Healing Time ◽

Skin Fibroblasts ◽

Human Skin Fibroblasts ◽

Protein Levels ◽

Daphne Genkwa

Wound healing is the main problem in the therapy of anal fistula (AF). Daphne genkwa root has been traditionally used as an agent to soak sutures in operation of AF patients, but its function in wound healing remains largely unclear. The aim of the present study was to illuminate mechanisms of D. genkwa root treatment on AF. In the present study, 60 AF patients after surgery were randomly divided into two groups, external applied with or without the D. genkwa extractive. Wound healing times were compared and granulation tissues were collected. In vitro, we constructed damaged human skin fibroblasts (HSFs) with the treatment of TNF-α (10 μg/ml). Cell Count Kit-8 (CCK-8) and flow cytometry analysis were used to determine the effects of D. genkwa root extractive on cell viability, cell cycle and apoptosis of damaged HSFs. Furthermore, protein levels of TGF-β, COL1A1, COL3A1, Timp-1, matrix metalloproteinase (MMP)-3 (MMP-3) and MEK/ERK signalling pathways were investigated both in vivo and in vitro. Results showed that D. genkwa root extractive greatly shortens the wound healing time in AF patients. In granulation tissues and HSFs, treatment with the extractive significantly elevated the expressions of COL1A1, COL3A1, Timp-1, c-fos and Cyclin D1, while reduced the expression of MMP-3. Further detection presented that MEK/ERK signalling was activated after the stimulation of extractive in HSFs. Our study demonstrated that extractive from D. genkwa root could effectively improve wound healing in patients with AF via the up-regulation of fibroblast proliferation and expressions of COL1A1 and COL3A1.

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