Protective Effects of Myricetin on Benzo[a]pyrene-Induced 8-Hydroxy-2′-Deoxyguanosine and BPDE-DNA Adduct

Benzo[a]pyrene (B[a]P), a group 1 carcinogen, induces mutagenic DNA adducts. Myricetin is present in many natural foods with diverse biological activities, such as anti-oxidative and anti-cancer activities. The aim of this study was to investigate the protective effects of myricetin against B[a]P-induced toxicity. Treatment of B[a]P induced cytotoxicity on HepG2 cells, whereas co-treatment of myricetin with B[a]P reduced the formation of the B[a]P-7,8-dihydrodiol-9,10-epoxide (BPDE)-DNA adduct, which recovered cell viability. Furthermore, we found a protective effect of myricetin against B[a]P-induced genotoxicity in rats, via myricetin-induced inhibition of 8-hydroxy-2′-deoxyguanosine (8-OHdG) and BPDE-DNA adduct formation in the liver, kidney, colon, and stomach tissue. This inhibition was more prominent in the liver than in other tissues. Correspondingly, myricetin regulated the phase I and II enzymes that inhibit B[a]P metabolism and B[a]P metabolites conjugated with DNA by reducing and inducing CYP1A1 and glutathione S-transferase (GST) expression, respectively. Taken together, this showed that myricetin attenuated B[a]P-induced genotoxicity via regulation of phase I and II enzymes. Our results suggest that myricetin is anti-genotoxic, and prevents oxidative DNA damage and BPDE-DNA adduct formation via regulation of phase I and II enzymes.

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Oral administration of naturally occurring coumarins leads to altered phase I and II enzyme activities and reduced DNA adduct formation by polycyclic aromatic hydrocarbons in various tissues of SENCAR mice

Carcinogenesis ◽

10.1093/carcin/22.1.73 ◽

2001 ◽

Vol 22 (1) ◽

pp. 73-82 ◽

Cited By ~ 51

Author(s):

H. E. Kleiner

Keyword(s):

Polycyclic Aromatic Hydrocarbons ◽

Oral Administration ◽

Phase I ◽

Aromatic Hydrocarbons ◽

Enzyme Activities ◽

Adduct Formation ◽

Dna Adduct ◽

Naturally Occurring ◽

Polycyclic Aromatic ◽

Phase I And Ii

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Alteration in Phase I and II Enzyme Activities and Polycyclic Aromatic Hydrocarbons-DNA Adduct Formation by Plant Phenolics in Mouse Epidermis

Nutrition and Cancer ◽

10.1207/s15327914nc4801_10 ◽

2004 ◽

Vol 48 (1) ◽

pp. 70-77 ◽

Cited By ~ 38

Author(s):

Hanna Szaefer ◽

Michal Cichocki ◽

Damian Brauze ◽

Wanda Baer-Dubowska

Keyword(s):

Polycyclic Aromatic Hydrocarbons ◽

Phase I ◽

Aromatic Hydrocarbons ◽

Enzyme Activities ◽

Adduct Formation ◽

Dna Adduct ◽

Plant Phenolics ◽

Mouse Epidermis ◽

Polycyclic Aromatic ◽

Phase I And Ii

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Anti-Cancer and Protective Effects of Royal Jelly for Therapy-Induced Toxicities in Malignancies

International Journal of Molecular Sciences ◽

10.3390/ijms19103270 ◽

2018 ◽

Vol 19 (10) ◽

pp. 3270 ◽

Cited By ~ 7

Author(s):

Yasuyoshi Miyata ◽

Hideki Sakai

Keyword(s):

Molecular Mechanisms ◽

Royal Jelly ◽

Biological Activities ◽

Treatment Strategies ◽

Protective Effects ◽

Beneficial Effects ◽

Glandular Secretion ◽

Anti Cancer ◽

The Future ◽

Queen Honeybee

Royal jelly (RJ) is a glandular secretion produced by worker honeybees and is a special food for the queen honeybee. It results in a significant prolongation of the lifespan of the queen honeybee compared with the worker honeybees through anti-inflammatory, anti-oxidant and anti-microbial activities. Consequently, RJ is used as cosmetic and dietary supplement throughout the world. In addition, in vitro studies and animal experiments have demonstrated that RJ inhibits cell proliferation and stimulates apoptosis in various types of malignant cells and affects the production of various chemokines, anti-oxidants and growth factors and the expression of cancer-related molecules in patients with malignancies, especially in patients treated with anti-cancer agents. Therefore, RJ is thought to exert anti-cancer effects on tumor growth and exhibit protective functions against drug-induced toxicities. RJ has also been demonstrated to be useful for suppression of adverse events, the maintenance of the quality of life during treatment and the improvement of prognosis in animal models and patients with malignancies. To understand the mechanisms of the beneficial effects of RJ, knowledge of the changes induced at the molecular level by RJ with respect to cell survival, inflammation, oxidative stress and other cancer-related factors is essential. In addition, the effects of combination therapies of RJ and other anti-cancer agents or natural compounds are important to determine the future direction of RJ-based treatment strategies. Therefore, in this review, we have covered the following five issues: (1) the anti-cancer effects of RJ and its main component, 10-hydroxy-2-decenoic acid; (2) the protective effects of RJ against anti-cancer agent-induced toxicities; (3) the molecular mechanisms of such beneficial effects of RJ; (4) the safety and toxicity of RJ; and (5) the future directions of RJ-based treatment strategies, with a discussion on the limitations of the study of the biological activities of RJ.

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