scholarly journals Superior Antioxidant Capacity of Berberis iliensis—HPLC-Q-TOF-MS Based Phytochemical Studies and Spectrophotometric Determinations

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 504 ◽  
Author(s):  
Saniia Abdykerimova ◽  
Zuriyadda Sakipova ◽  
Sylwia Nakonieczna ◽  
Wojciech Koch ◽  
Anna Biernasiuk ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
High Performance ◽  
Glucaric Acid ◽  
Gram Positive Bacteria ◽  
Antioxidant Agent ◽  
Ethanol Ethanol ◽  
Phytochemical Studies ◽  
Acid Esters ◽  
Tof Ms

The aim of the present study was to determine the composition, antiradical and antimicrobial activity of fruits, leaves and roots of an underestimated species of barberry—Berberis iliensis—growing in Kazakhstan. Particular attention was paid to the determination of the composition of its extracts by high-performance liquid chromatography coupled with mass spectrometry (HPLC-ESI-Q-TOF-MS) analysis. As a result of the chromatographic and spectrometric study 33 secondary metabolites from the groups of phenolic acids and their esters, flavonoids, alkaloids and organic acids were identified and 15 of them—quantified. The isomers of caffeoyl-glucaric acid, caffeic acid derivatives, isoquercetin, berberine and jatrorrhizine were the most abundant components of the tested extracts. The antiradical activity tests were performed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Folin-Ciocalteu assays on four types of extracts (water, ethanol, ethanol-water 7:3 v/v, ethanol-water 1:1 v/v) from the three organs of the plant. The highest antiradical potential (IC50 = 80 ± 6.36 µg/mL) and phenolic content (440 ± 17.1 mg gallic acid equivalents/L) was calculated for ethanol- water (1:1 v/v) extracts from the leaves and could be influenced by the abundant presence of simple phenolic acids, flavonoids and glucaric acid esters. Among reference microorganisms, M. luteus, S. epidermidis, some S. aureus and B. cereus belonging to Gram-positive bacteria and yeasts from Candida species were the most sensitive to roots extract that was found the most active among the studied samples. The results of the study classify Berberis iliensis as a strong antioxidant agent and as a plant with an antimicrobial potential.

scholarly journals Determination of Triterpenoids and Phenolic Acids from Sanguisorba officinalis L. by HPLC-ELSD and Its Application

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4505
Author(s):  
Jiahui Sun ◽  
Chunli Gan ◽  
Jing Huang ◽  
Zhenyue Wang ◽  
Chengcui Wu ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
High Performance ◽  
Gradient Elution ◽  
Light Scattering Detection ◽  
Evaporative Light Scattering ◽  
Acetic Acid Water ◽  
Sanguisorba Officinalis ◽  
Functional Components ◽  
Gradient Elution Mode

A novel analytical method involving high-performance liquid chromatography with evaporative light scattering detection (HPLC-ELSD) was developed for simultaneous determination of 11 phenolic acids and 12 triterpenes in Sanguisorba officinalis L. Chromatographic separation was conducted with gradient elution mode by using a DiamonsilTM C18 column (250 mm × 4.6 mm, 5 μm) with the mobile phase of 0.1% acetic acid water (A) and methanol (B). The drift tube temperature of ELSD was set at 70 °C and the nitrogen cumulative flow rate was 1.6 L/min. The method was fully validated to be linear over a wide concentration range (R2 ≥ 0.9991). The precisions (RSD) were less than 3.0% and the recoveries were between 97.7% and 101.4% for all compounds. The results indicated that this method is accurate and effective for the determination of 23 functional components in Sanguisorba officinalis L. and could also be successfully applied to study the influence of processing method on those functional components in Sanguisorba officinalis L.

scholarly journals Diversity of Eleutherococcus genus in respect of biologically active compounds accumulation

2014 ◽  
Vol 60 (3) ◽  
pp. 34-43 ◽  
Author(s):  
Katarzyna Bączek
Keyword(s):  
High Performance Liquid Chromatography ◽  
Phenolic Acids ◽  
High Performance ◽  
Raw Materials ◽  
Stem Bark ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Active Compounds ◽  
Eleutheroside B

Summary Eight species of Eleutherococcus genus grown at Rogów Arboretum collection were compared in respect of biologically active compounds accumulation, i.e. eleutheroside B and E, phenolic acids and sterols. For the determination of content of these compounds in underground organs and stem bark high performance liquid chromatography was applied. The highest content of the sum of eleutherosides B and E was observed in underground organs and stem bark of E. leucorrhizus (322.0 and 300.8 mg × 100 g-1, respectively) and E. nodiflorus (218.9 and 363.5 mg × 100g-1, respectively). In the raw materials from E. senticosus, the content of these compounds was significantly lower (177.4 and 159.3 mg × 100 g-1, respectively). E divaricatus and E. setchuenensis were characterized by the lowest accumulation of these compounds in underground organs whereas E. divaricatus, E. sessiliflorus and E. giraldii - in stem bark. Four phenolic acids were identified in the investigated species, namely: chlorogenic, rosmarinic, ferulic and caffeic acids. The main phenolic acid in the analyzed species was chlorogenic acid. The content of this compound in underground organs varied from 102.1 (E. henryi) to 958.7 mg × 100 g-1 (E. leucorrhizus) and in stem bark from 26.7 (E. giraldii) to 542.5 mg × 100 g-1 (E. setchuenensis). The content of identified sterol compounds (sitosterol 3-O-β-D-glucoside /eleutheroside A / campesterol, stigmasterol) was relatively low, but higher in underground organs in comparison with stem bark.

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