scholarly journals Searching for Appropriate Storage Conditions for Short-Term Wet Preservation of Porphyridium purpureum

2020 ◽  
Vol 10 (23) ◽  
pp. 8315
Author(s):  
Joran Verspreet ◽  
Lise Soetemans ◽  
Leen Bastiaens
Keyword(s):  
Organic Matter ◽  
Storage Conditions ◽  
Threshold Values ◽  
Gel Structure ◽  
Short Term ◽  
Porphyridium Purpureum ◽  
First Case ◽  
Carbohydrate Degradation ◽  
Viscosity Increase ◽  
Weak Gel

It is often impossible in practice to process micro-algae immediately after their cultivation and harvest. This study, therefore, aimed to identify appropriate storage conditions for the wet preservation of Porphyridium purpureum. Algae were stored either as a concentrate or as a dilute culture at 4 °C, 8 °C, or 20 °C for 14 days and their quality was monitored. Concentrate storage tended to result in higher microbial numbers than dilute culture storage and clearly led to higher concentrations of malodorous organic acids. Butyric and isovaleric acid concentrations were about two orders of magnitude larger than their odor threshold values after 14 days of concentrate storage at 20 °C. Average B-phycoeryhrin (B-PE) levels were slightly higher after concentrate storage (2.5 ± 0.2 g B-PE/100 g organic matter) than after dilute culture storage (2.2 ± 0.5 g B-PE/100 g organic matter), probably due to respiration losses of other organic compounds in the first case. Significant amounts of organic matter got lost during concentrate storage (4–35%) as a result of carbohydrate degradation. The main restriction of concentrate storage was the rapid viscosity increase and formation of a weak gel structure complicating the later processing of the concentrates. These findings are highly relevant for P. purpureum cultivators and processors who have to store Porphyridium suspensions, even on a term of one day or less.

scholarly journals Short-Term Prediction of COVID-19 Cases Using Machine Learning Models

2021 ◽  
Vol 11 (9) ◽  
pp. 4266
Author(s):  
Md. Shahriare Satu ◽  
Koushik Chandra Howlader ◽  
Mufti Mahmud ◽  
M. Shamim Kaiser ◽  
Sheikh Mohammad Shariful Islam ◽  
...  
Keyword(s):  
Machine Learning ◽  
Web Application ◽  
Learning Models ◽  
Short Term ◽  
Infected People ◽  
Infected Case ◽  
First Case ◽  
Novel Coronavirus ◽  
Short Term Prediction ◽  
Machine Learning Models

The first case in Bangladesh of the novel coronavirus disease (COVID-19) was reported on 8 March 2020, with the number of confirmed cases rapidly rising to over 175,000 by July 2020. In the absence of effective treatment, an essential tool of health policy is the modeling and forecasting of the progress of the pandemic. We, therefore, developed a cloud-based machine learning short-term forecasting model for Bangladesh, in which several regression-based machine learning models were applied to infected case data to estimate the number of COVID-19-infected people over the following seven days. This approach can accurately forecast the number of infected cases daily by training the prior 25 days sample data recorded on our web application. The outcomes of these efforts could aid the development and assessment of prevention strategies and identify factors that most affect the spread of COVID-19 infection in Bangladesh.

scholarly journals Impact of Thermotherapy and Short-Term Storage on Quercus robur L. Acorn Mycobiota and Germination

Forests ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 528
Author(s):  
Jelena Kranjec Orlović ◽  
Damir Drvodelić ◽  
Marko Vukelić ◽  
Matea Rukavina ◽  
Danko Diminić ◽  
...  
Keyword(s):  
Natural Regeneration ◽  
Fungal Diversity ◽  
Quercus Robur ◽  
Negative Impact ◽  
Storage Conditions ◽  
Short Term ◽  
Isolation Frequency ◽  
Short Term Storage ◽  
Penicillium Spp ◽  
Term Storage

When natural regeneration of Quercus robur stands is hampered by an insufficient acorn yield, human assisted sowing of acorns collected in non-affected stands and stored for some period of time is performed. To inhibit the development of fungi and acorn deterioration during storage, thermotherapy is usually applied by submerging acorns for 2.5 h in water heated to 41 °C. This research aimed to test the effect of four thermotherapy treatments of different durations and/or applied temperatures as well as short-term storage at −1 °C or 3 °C on acorn internal mycobiota and germination. Fungal presence in cotyledons was analyzed in 450 acorns by isolation of mycelia on artificial media, followed by a DNA-based identification. Germination of 2000 acorns was monitored in an open field trial. Thermotherapy significantly decreased fungal diversity, while storage at 3 °C increased the isolation frequency of several fungi, mainly Penicillium spp. The most frequently isolated fungi did not show a negative impact on acorn germination after short-term storage. The study confirmed the efficiency of thermotherapy in the eradication of a part of acorn internal mycobiota, but also its effect on the proliferation of fast-colonizing fungi during storage. However, the latter showed to be more stimulated by storage conditions, specifically by storage at 3 °C.

Calcaneal Reconstruction With Free Deep Circumflex Iliac Artery Osseocutaneous Flap Following Aggressive Benign Bone Tumor Resection

2021 ◽  
pp. 107110072110252
Author(s):  
Mohamad Aizat Rosli ◽  
Wan Faisham Wan Ismail ◽  
Wan Azman Wan Sulaiman ◽  
Nor Azman Mat Zin ◽  
Syurahbil Abdul Halim ◽  
...  
Keyword(s):  
Iliac Artery ◽  
Donor Site ◽  
Tumor Resection ◽  
Case Series ◽  
Benign Tumors ◽  
Reconstruction Method ◽  
Short Term ◽  
Deep Circumflex Iliac Artery ◽  
First Case ◽  
Donor Site Pain

Background: Advances in limb-salvage techniques have made total calcanectomy and primary reconstruction possible in managing calcaneal aggressive benign tumors and selected cases of intraosseous malignant tumors. However, there is still no consensus on the operative approach, oncologic margin, and the best reconstruction method to date. These 2 cases describe our experience in calcaneal reconstruction with the free deep circumflex iliac artery (DCIA) osseocutaneous flap in benign aggressive calcaneal tumors. Methods: We reported 2 consecutive male and female patients, with an average age of 25 years (age 19 and 31, respectively), who underwent total calcanectomy and primary calcaneal reconstruction with the free DCIA osseocutaneous flaps for calcaneal chondroblastoma and giant cell tumor. A marginal resection of the entire calcaneus through the subtalar and calcaneocuboid joints (intra-articular approach) was performed in the first case and a wide local resection leaving 1 cm normal calcaneal bone margin anterosuperiorly (intraosseous approach) was performed in the second case. Results: The follow-up period averaged 48 months. Negative oncologic margins were achieved in both cases. The first case was complicated with venous thrombosis; however, the graft remained viable after emergency reexploration. Normal foot function was restored with good solid osseous union and bony hypertrophy observed. Both patients achieved good short-term functional and aesthetic outcomes with no donor site pain or disability. No local recurrence was reported either. Conclusion: Primary calcaneal reconstruction with the free DCIA osseocutaneous flap can lead to good short-term functional and aesthetic outcomes. Level of Evidence: Level IV, case series.

scholarly journals Short-Term Decomposition and Nutrient-Supplying Ability of Sewage Sludge Digestate, Digestate Compost, and Vermicompost on Acidic Sandy and Calcareous Loamy Soils

Agronomy ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 2249
Author(s):  
Nikolett Uzinger ◽  
Orsolya Szécsy ◽  
Nóra Szűcs-Vásárhelyi ◽  
István Padra ◽  
Dániel Benjámin Sándor ◽  
...  
Keyword(s):  
Organic Matter ◽  
Sewage Sludge ◽  
Organic Carbon ◽  
Carbon Content ◽  
Plant Biomass ◽  
Test Plant ◽  
Organic Carbon Content ◽  
Short Term ◽  
Mineral N ◽  
Average Residual

Organic waste and the compost and vermicompost derived from it may have different agronomic values, but little work is available on this aspect of sewage sludge. A 75-day pot experiment with perennial ryegrass (Lolium perenne) as the test plant aimed to investigate the fertiliser value and organic matter replenishment capacity of digested sewage sludge (DS) and the compost (COM) and vermicompost (VC) made from it, applied in 1% and 3% doses on acidic sand and calcareous loam. The NPK content and availability, changes in organic carbon content and plant biomass, and the efficiency of the amendments as nitrogen fertilisers were investigated. The final average residual carbon content for DS, COM, and VC was 35 ± 34, 85 ± 46, and 55 ± 46%, respectively. The organic carbon mineralisation rate depended on the soil type. The additives induced significant N mineralisation in both soils: the average increment in mineral N content was 1.7 times the total added N on acidic sand and 4.2 times it on calcareous loam for the 1% dose. The agronomic efficiency of COM and VC as fertilisers was lower than that of DS. In the short term, DS proved to be the best fertiliser, while COM was the best for organic matter replenishment.

Galeazzi-equivalent Fractures: Report of Two Cases and Literature Review

2017 ◽  
Vol 22 (02) ◽  
pp. 232-235
Author(s):  
Seigo Suganuma ◽  
Kaoru Tada ◽  
Daiki Yamamoto ◽  
Hiroyuki Tsuchiya
Keyword(s):  
Emergency Surgery ◽  
Case Reports ◽  
Growth Arrest ◽  
Short Term ◽  
Rare Injury ◽  
First Case ◽  
Long Term Follow Up ◽  
Epiphyseal Injury

The Galeazzi-equivalent fracture is a rare injury that occurs in children. The most important issue is the distal ulnar epiphyseal injury. Although there have been some case reports, most of them performed only short term follow-up. This article describes two cases of this fracture with long term follow-up until epiphyseal closure. First case is a 12-year-old girl who sustained a Galeazziequivalent fracture of her right forearm and underwent emergency surgery. At follow-up of 5 years and 10 months postsurgery, radiographs show ulnar growth arrest of one mm and she has mild pain. Second case is a 15-year-old boy who sustained an open Galeazzi-equivalent fracture of his left forearm and underwent emergency surgery. At follow-up of 3 years and 3 months postsurgery, radiographs show no growth arrest of the distal ulna. He has no residual complaint. Long term follow-up is absolutely necessary to monitor ulnar growth.

Dilemmas of an Expert Witness

2018 ◽  
Author(s):  
Stuart Kirsch
Keyword(s):  
Open Access ◽  
Land Tenure ◽  
Expert Witness ◽  
Land Rights ◽  
The Political ◽  
Ethnographic Research ◽  
Negative Consequences ◽  
Short Term ◽  
First Case ◽  
Indigenous Land

This chapter presents two affidavits submitted to the Inter-American Court. The first case was concerned with the negative consequences of Suriname’s refusal to recognize indigenous land rights, including the establishment of a nature reserve that become a de facto open-access zone on indigenous land. The second addressed problems associated with indigenous land tenure in Guyana under the Amerindian Act of 2006. Comparing the two cases allows the chapter to make several observations about the dynamics of short-term ethnographic research conducted for expert-witness reports. This includes the need to make affidavits legible to the three overlapping frames of the legal system, the communities seeking recognition of their rights, and anthropology. The chapter also considers the narrative choices in these affidavits, the political dilemmas of being an expert witness, and the compromises of short-term ethnography.

Should Case Materials Precede or Follow Lectures?

2004 ◽  
Vol 19 (3) ◽  
pp. 305-319 ◽  
Author(s):  
Fred Phillips ◽  
Ganesh Vaidyanathan
Keyword(s):  
Case Analysis ◽  
Fundamental Question ◽  
Controlled Experiment ◽  
Short Term ◽  
First Case ◽  
Based Instruction ◽  
Practical Implications

This paper examines a fundamental question faced by all accounting educators who use accounting case analyses alongside lecture-based instruction: Does it matter whether cases precede or follow a lecture? Results from a controlled experiment indicate that students' case analysis performance is initially enhanced when a lecture precedes a case, because the lecture equips students with knowledge to apply to the case and it constrains the number of irrelevant ideas that students apply to the case. However, there is a drawback to positioning a lecture before the first case—it constrains the number of relevant ideas that students generate themselves to apply to the case. In addition, the short-term benefits of positioning a lecture before case analyses disappear when students analyze a second case. Specifically, we find that performance on a second case is significantly better when students have previously learned in an environment that places the first case before the lecture. Our evidence suggests that these case-before-lecture students are more likely to engage their pre-lecture knowledge and the knowledge obtained from the lecture when analyzing the second case. Practical implications for using cases with lectures are discussed.

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