Environmental Impact of Sulfate-Reducing Bacteria, Their Role in Intestinal Bowel Diseases, and Possible Control by Bacteriophages

Sulfate-reducing bacteria (SRB) represent a group of prokaryotic microorganisms that are widely spread in the anoxic environment (seabed, riverbed and lakebed sediments, mud, intestinal tract of humans and animals, metal surfaces). SRB species also have an impact on processes occurring in the intestinal tract of humans and animals, including the connections between their presence and inflammatory bowel disease (IBD). Since these SRB can develop antimicrobial resistance toward the drugs, including antibiotics and antimicrobial agents, bacteriophages could represent an additional potential effective treatment. The main objectives of the review were as follows: (a) to review SRB (both from intestinal and environmental sources) regarding their role in intestinal diseases as well as their influence in environmental processes; and (b) to review, according to literature data, the influence of bacteriophages on SRB and their possible applications. Since SRB can have a significant adverse influence on industry as well as on humans and animals health, phage treatment of SRB can be seen as a possible effective method of SRB inhibition. However, there are relatively few studies concerning the influence of phages on SRB strains. Siphoviridae and Myoviridae families represent the main sulfide-producing bacteria phages. The most recent studies induced, by UV light, bacteriophages from Desulfovibrio vulgaris NCIMB 8303 and Desulfovibrio desulfuricans ATCC 13541. Notwithstanding costly and medically significant negative impacts of phages on SRB, they have been the subject of relatively few studies. The current search for alternatives to chemical biocides and antibiotics has led to the renewed interest in phages as antibacterial biocontrol and therapeutic agents, including their use against SRB. Hence, phages might represent a promising treatment against SRB in the future.

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Experimental Investigation on the Active Range of Sulfate-Reducing Bacteria for Geological Disposal

MRS Proceedings ◽

10.1557/proc-353-173 ◽

1994 ◽

Vol 353 ◽

Cited By ~ 3

Author(s):

S. Fukunaga ◽

H. Yoshikawa ◽

K. Fujiki ◽

H. Asano

Keyword(s):

Experimental Investigation ◽

Environmental Conditions ◽

Desulfovibrio Desulfuricans ◽

Sulfate Reducing Bacteria ◽

Geological Disposal ◽

Sulfate Reducing ◽

Buffer Material ◽

Ph Range ◽

High Level ◽

Reducing Bacteria

AbstractThe active range ofDesulfovibrio desulfuricans. a species of sulfate-reducing bacteria, was examined in terms of pH and Eh using a fermenter at controlled pH and Eh. Such research is important because sulfate-reducing bacteria (SRB) are thought to exist underground at depths equal to those of supposed repositories for high-level radioactive wastes and to be capable of inducing corrosion of the metals used in containment vessels.SRB activity was estimated at 35°C, with lactate as an electron donor, at a pH range from 7 to 11 and Eh range from 0 to -380 mV. Activity increased as pH approached neutral and Eh declined. The upper pH limit for activity was between 9.9 and 10.3, at Eh of -360 to -384 mV. The upper Eh limit for activity was between -68 and -3 mV, at pH 7.1. These results show that SRB can be made active at higher pH by decreasing Eh, and that the higher pH levels of 8 to 10 produced by use of the buffer material bentonite does not suppress SRB completely.A chart was obtained showing the active range ofDesulfovibrio desulfuricansin terms of pH and Eh. Such charts can be used to estimate the viability of SRB and other microorganisms when the environmental conditions of a repository are specified.

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Biochemical Studies on Sulfate-reducing BacteriaXII. Some Properties of Flavodoxin from Desulfovibrio vulgaris

The Journal of Biochemistry ◽

10.1093/oxfordjournals.jbchem.a130092 ◽

1973 ◽

Keyword(s):

Sulfate Reducing Bacteria ◽

Desulfovibrio Vulgaris ◽

Sulfate Reducing ◽

Biochemical Studies ◽

Reducing Bacteria

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Microbial Corrosion of API 5L X-70 Carbon Steel by ATCC 7757 and Consortium of Sulfate-Reducing Bacteria

Journal of Chemistry ◽

10.1155/2014/130345 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 11

Author(s):

Arman Abdullah ◽

Nordin Yahaya ◽

Norhazilan Md Noor ◽

Rosilawati Mohd Rasol

Keyword(s):

Weight Loss ◽

Carbon Steel ◽

Corrosion Rate ◽

Sulfate Reducing Bacteria ◽

Desulfovibrio Vulgaris ◽

Corrosion Morphology ◽

Sulfate Reducing ◽

Weight Loss Method ◽

Loss Method ◽

Reducing Bacteria

Various cases of accidents involving microbiology influenced corrosion (MIC) were reported by the oil and gas industry. Sulfate reducing bacteria (SRB) have always been linked to MIC mechanisms as one of the major causes of localized corrosion problems. In this study, SRB colonies were isolated from the soil in suspected areas near the natural gas transmission pipeline in Malaysia. The effects of ATCC 7757 and consortium of isolated SRB upon corrosion on API 5L X-70 carbon steel coupon were investigated using a weight loss method, an open circuit potential method (OCP), and a potentiodynamic polarization curves method in anaerobic conditions. Scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS) were then used to determine the corrosion morphology in verifying the SRB activity and corrosion products formation. Results from the study show that the corrosion rate (CR) of weight loss method for the isolated SRB is recorded as 0.2017 mm/yr compared to 0.2530 mm/yr for ATCC 7757. The Tafel plot recorded the corrosion rate of 0.3290 mm/yr for Sg. Ular SRB and 0.2500 mm/yr forDesulfovibrio vulgaris. The results showed that the consortia of isolated SRB were of comparable effects and features with the single ATCC 7757 strain.

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A comparison of the electrophoretic properties of the ATP-sulfurylases, APS-reductases, and sulfite reductases from cultures of dissimilatory sulfate-reducing bacteria

Canadian Journal of Microbiology ◽

10.1139/m73-061 ◽

1973 ◽

Vol 19 (3) ◽

pp. 375-380 ◽

Cited By ~ 10

Author(s):

G. W. Skyring ◽

P. A. Trudinger

Keyword(s):

Desulfovibrio Desulfuricans ◽

Sulfate Reducing Bacteria ◽

Disc Electrophoresis ◽

Sulfite Reductase ◽

Electrophoretic Variation ◽

Sulfate Reducing ◽

Aps Reductase ◽

Reducing Bacteria ◽

Atp Sulfurylases

ATP-sulfurylases, APS-reductases, and sulfite reductases (SO3−2 → S−2) have been detected by gel disc electrophoresis in 13 cultures of dissimilatory sulfate-reducing bacteria and their electrophoretic properties have been compared. With respect to these three enzymes only, the results were indicative of some interspecies and intergenus homologies. In the Desulfovibrio strains (except Desulfovibrio desulfuricans 8301 which does not contain desulfoviridin), the major sulfite reductase was electrophoretically coincident with desulfoviridin and, in the Desulfotomaculum strains, with a brown protein. Some distinct patterns of electrophoretically distinguishable forms of APS-reductase were found. Considerable electrophoretic variation was found among the ATP-sulfurylases.

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Contribution of Sulfate-Reducing Bacteria to the Electricity Generation in Microbial Fuel Cells

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.1008-1009.285 ◽

2014 ◽

Vol 1008-1009 ◽

pp. 285-289 ◽

Cited By ~ 2

Author(s):

Chong Yang Gao ◽

Ai Jie Wang ◽

Yang Guo Zhao

Keyword(s):

Fuel Cells ◽

Power Density ◽

Microbial Fuel Cells ◽

Electricity Generation ◽

Desulfovibrio Desulfuricans ◽

Sulfate Reducing Bacteria ◽

Microbial Community Analysis ◽

Electricity Production ◽

Sulfate Reducing ◽

Reducing Bacteria

Double-chambered microbial fuel cells (MFCs) were used to investigate the effect of sulfate and sulfate-reducing bacteria (SRB) on electricity generation by molybdate inhibition coupled with PCR-DGGE technique. Results showed that low influent sulfate (< 1470 mg/L) improved power density and voltage, while higher sulfate blocked the MFC efficiency. Molybdate inhibited the activity of SRB and consequently decreased MFC voltage and power density which confirmed some SRB were involved in the electricity generation. Microbial community analysis indicated thatDesulfovibrio desulfuricanscontributed to the electricity production and stability of MFC.

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Response of Desulfovibrio desulfuricans colonies to oxygen stress

Canadian Journal of Microbiology ◽

10.1139/m90-070 ◽

1990 ◽

Vol 36 (6) ◽

pp. 400-408 ◽

Cited By ~ 9

Author(s):

Judy D. Wall ◽

Barbara J. Rapp-Giles ◽

Merton F. Brown ◽

Jerry A. White

Keyword(s):

Electron Microscopy ◽

Morphological Changes ◽

Metal Sulfide ◽

Desulfovibrio Desulfuricans ◽

Sulfate Reducing Bacteria ◽

Strictly Anaerobic ◽

Air Oxidation ◽

Oxygen Tolerance ◽

Sulfate Reducing ◽

Reducing Bacteria

Oxygen tolerance of the strictly anaerobic sulfate-reducing bacteria is well documented and poorly understood. This capacity for surviving brief exposures to oxygen must be a major factor in the diversity of environmental niches observed for these bacteria. We observed that viable cells of Desulfovibrio desulfuricans (ATCC 27774) could be found in colonies on the surface of solidified medium exposed to air for periods as long as 1 month. During exposure to air, the originally black colonies became greyish white, presumably as a result of the air oxidation of the metal sulfide deposits. A black, brittle deposit formed at the bottom of the colony and, simultaneously, the colony descended into a dimple that developed into a well in the agar. Eventually the colony reached the bottom of the Petri dish. These changes did not take place when the colonies were maintained in an anaerobic chamber. The morphological changes took place with all strains tested: three strains of D. desulfuricans and one strain of Desulfovibrio gigas and Desulfovibrio multispirans. Continued sulfate reduction appeared to be essential. Cyclic sulfate (thiosulfate or sulfite) reduction to sulfide and reoxidation of sulfide by the oxygen in air are proposed to maintain the viability of the bacteria by providing substrates for energy production and by reducing oxygen tension. Scanning and transmission electron microscopy of colony and cellular changes are shown. Key words: Desulfovibrio, sulfate-reducing bacteria, oxygen tolerance, sulfate cycling, scanning electron microscopy.

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Reduction of perchlorate ions by the sulfate-reducing bacteria Desulfotomaculum sp. and Desulfovibrio desulfuricans

Regulatory Mechanisms in Biosystems ◽

10.15421/022041 ◽

2020 ◽

Vol 11 (2) ◽

pp. 278-282

Author(s):

N. S. Verkholiak ◽

T. B. Peretyatko ◽

A. A. Halushka

Keyword(s):

Sewage Treatment ◽

Desulfovibrio Desulfuricans ◽

Sulfate Reducing Bacteria ◽

Inhibitory Effect ◽

Electron Acceptors ◽

Wood Chips ◽

Sulfate Ions ◽

Sulfate Reducing ◽

Perchlorate Ion ◽

Reducing Bacteria

The usage of microorganisms to clean the environment from xenobiotics, in particular chlorine-containing ones, is a promising method of detoxifying the contaminated environment. Sulfate-reducing bacteria Desulfovibrio desulfuricans Ya-11, isolated from Yavoriv Lake, and Desulfotomaculum AR1, isolated from the Lviv sewage treatment system, are able to grow under conditions of environmental contamination by aromatic compounds and chlorine-containing substances. Due to their high redox potential, chlorate and perchlorate ions can be ideal electron acceptors for the metabolism of microorganisms. To test the growth of the tested microorganisms under the influence of perchlorate ions, bacteria were cultured in modified Postgate C medium with ClO4–. Biomass was determined turbidimetrically, the content of sulfate ions and hydrogen sulfide – photoelectrocolorimetrically, the content of perchlorate ions – permanganatometrically. The study of the ability of sulfate-reducing bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 to grow in a medium with perchlorate ions as electron acceptors showed the inhibitory effect of ClO4– on sulfate ion reduction by bacteria. Bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 are able to grow in environments with aromatic hydrocarbons, in particular toluene. The possibility of the growth of sulfate-reducing bacteria in the presence of toluene as an electron donor and perchlorate ions as an electron acceptor was investigated. The efficiency of perchlorate ion utilization by sulfate-reducing bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 was about 90 %. The effect of molybdenum on the reduction of perchlorate ions by Desulfotomaculum AR1 is shown in the paper. Immobilization of bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 was carried out in 3% agar and on wood chips. The ability of bacteria, immobilized on these media, to purify the aqueous medium from perchlorate ions was investigated. Reduction of perchlorate ions is more efficiently performed by cells of Desulfotomaculum AR1 and D. desulfuricans Ya-11 bacteria immobilized in agar than on wood chips. Sulfate-reducing bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 are able to use perchlorate ions as electron acceptors, purifying the polluted aquatic environment from these pollutants.

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A preliminary characterization of mercury uptake by the sulfate-reducing bacteria Desulfovibrio desulfuricans

10.25148/etd.fi14051132 ◽

2011 ◽

Author(s):

Amaury Pedro Betancourt

Keyword(s):

Desulfovibrio Desulfuricans ◽

Sulfate Reducing Bacteria ◽

Preliminary Characterization ◽

Sulfate Reducing ◽

Mercury Uptake ◽

Reducing Bacteria

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Kinetic properties of pyruvate ferredoxin oxidoreductase of intestinal sulfate-reducing bacteria Desulfovibrio piger Vib-7 and Desulfomicrobium sp. Rod-9

Polish Journal of Microbiology ◽

10.33073/pjm-2015-016 ◽

2015 ◽

Vol 64 (2) ◽

pp. 107-114 ◽

Cited By ~ 23

Author(s):

IVAN V. KUSHKEVYCH

Keyword(s):

Enzyme Reaction ◽

Sulfate Reducing Bacteria ◽

Kinetic Properties ◽

Bacterial Strains ◽

Ferredoxin Oxidoreductase ◽

Sulfate Reducing ◽

Bowel Diseases ◽

Inflammatory Bowel ◽

Pyruvate Ferredoxin Oxidoreductase ◽

Reducing Bacteria

Intestinal sulfate-reducing bacteria reduce sulfate ions to hydrogen sulfide causing inflammatory bowel diseases of humans and animals. The bacteria consume lactate as electron donor which is oxidized to acetate via pyruvate in process of the dissimilatory sulfate reduction. Pyruvate-ferredoxin oxidoreductase activity and the kinetic properties of the enzyme from intestinal sulfate-reducing bacteria Desulfovibrio piger and Desulfomicrobium sp. have never been well-characterized and have not been yet studied. In this paper we present for the first time the specific activity of pyruvate-ferredoxin oxidoreductase and the kinetic properties of the enzyme in cell-free extracts of both D. piger Vib-7 and Desulfomicrobium sp. Rod-9 intestinal bacterial strains. Microbiological, biochemical, biophysical and statistical methods were used in this work. The optimal temperature (+35°C) and pH 8.5 for enzyme reaction were determined. The spectral analysis of the puri- fied pyruvate-ferredoxin oxidoreductase from the cell-free extracts was demonstrated. Analysis of the kinetic properties of the studied enzyme was carried out. Initial (instantaneous) reaction velocity (V0), maximum amount of the product of reaction (Pmax), the reaction time (half saturation period) and maximum velocity of the pyruvate-ferredoxin oxidoreductase reaction (V ) were defined. Michaelis constants (Km) of the enzyme reaction were calculated for both intestinal bacterial strains. The studies of the kinetic enzyme properties in the intestinal sulfate-reducing bacteria strains in detail can be prospects for clarifying the etiological role of these bacteria in the development of inflammatory bowel diseases.

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