scholarly journals Fluorescence Lifetime Phasor Analysis and Raman Spectroscopy of Pigmented Organic Binders and Coatings Used in Artworks

2021 ◽  
Vol 12 (1) ◽  
pp. 179
Author(s):  
Alice Dal Fovo ◽  
Sara Mattana ◽  
Antonina Chaban ◽  
Diego Quintero Balbas ◽  
João Luis Lagarto ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Fluorescence Lifetime ◽  
Fluorescence Analysis ◽  
Data Interpretation ◽  
Chemical Information ◽  
Natural Resin ◽  
Materials Used ◽  
Dispersing Medium ◽  
Phasor Analysis

Fluorescence analysis of materials used as binders and coatings in artworks is often hampered by numerous factors, leading to uncertainties in data interpretation. Fluorescence lifetime (FL) measurements enable improvement of the specificity with respect to steady-state measurements by resolving the decay dynamics of the fluorophore emissions. In this work, layers of natural resin, oil, and wax—in pure form, pigmented, in mixtures, and spread on different substrates—were analyzed using a compact, portable, fiber-based FL instrument. FL data were processed via the phasor method and integrated with Raman spectroscopy to obtain complementary chemical information on the different substances. It was observed that the τ-phase of the mixtures is affected by both the pigments and the dispersing medium, and that the presence of the metal substrate contributes to changes in the FL of mixtures. The results obtained with our portable FL system combined with Raman spectroscopy pave the way for a systematic study of a larger number of materials for future in situ applications on works of art.

scholarly journals In situ Raman mapping of art objects

2016 ◽  
Vol 374 (2082) ◽  
pp. 20160039 ◽  
Author(s):  
D. Lauwers ◽  
Ph. Brondeel ◽  
L. Moens ◽  
P. Vandenabeele
Keyword(s):  
Raman Spectroscopy ◽  
Spatial Distribution ◽  
Chemical Information ◽  
Raman Mapping ◽  
In Situ Raman ◽  
Art Objects ◽  
Mapping System ◽  
Non Destructive ◽  
Raman Image

Raman spectroscopy has grown to be one of the techniques of interest for the investigation of art objects. The approach has several advantageous properties, and the non-destructive character of the technique allowed it to be used for in situ investigations. However, compared with laboratory approaches, it would be useful to take advantage of the small spectral footprint of the technique, and use Raman spectroscopy to study the spatial distribution of different compounds. In this work, an in situ Raman mapping system is developed to be able to relate chemical information with its spatial distribution. Challenges for the development are discussed, including the need for stable positioning and proper data treatment. To avoid focusing problems, nineteenth century porcelain cards are used to test the system. This work focuses mainly on the post-processing of the large dataset which consists of four steps: (i) importing the data into the software; (ii) visualization of the dataset; (iii) extraction of the variables; and (iv) creation of a Raman image. It is shown that despite the challenging task of the development of the full in situ Raman mapping system, the first steps are very promising. This article is part of the themed issue ‘Raman spectroscopy in art and archaeology’.

scholarly journals Raman Imaging and Fluorescence Lifetime Imaging Microscopy for Diagnosis of Cancer State and Metabolic Monitoring

Cancers ◽  
2021 ◽  
Vol 13 (22) ◽  
pp. 5682
Author(s):  
Lucas Becker ◽  
Nicole Janssen ◽  
Shannon L. Layland ◽  
Thomas E. Mürdter ◽  
Anne T. Nies ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Fluorescence Lifetime ◽  
Drug Efficacy ◽  
Raman Microspectroscopy ◽  
Fluorescence Lifetime Imaging Microscopy ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Non Invasive

Hurdles for effective tumor therapy are delayed detection and limited effectiveness of systemic drug therapies by patient-specific multidrug resistance. Non-invasive bioimaging tools such as fluorescence lifetime imaging microscopy (FLIM) and Raman-microspectroscopy have evolved over the last decade, providing the potential to be translated into clinics for early-stage disease detection, in vitro drug screening, and drug efficacy studies in personalized medicine. Accessing tissue- and cell-specific spectral signatures, Raman microspectroscopy has emerged as a diagnostic tool to identify precancerous lesions, cancer stages, or cell malignancy. In vivo Raman measurements have been enabled by recent technological advances in Raman endoscopy and signal-enhancing setups such as coherent anti-stokes Raman spectroscopy or surface-enhanced Raman spectroscopy. FLIM enables in situ investigations of metabolic processes such as glycolysis, oxidative stress, or mitochondrial activity by using the autofluorescence of co-enzymes NADH and FAD, which are associated with intrinsic proteins as a direct measure of tumor metabolism, cell death stages and drug efficacy. The combination of non-invasive and molecular-sensitive in situ techniques and advanced 3D tumor models such as patient-derived organoids or microtumors allows the recapitulation of tumor physiology and metabolism in vitro and facilitates the screening for patient-individualized drug treatment options.

Electrochemical Tip-Enhanced Raman Spectroscopy: An In Situ Nanospectroscopy for Electrochemistry

2021 ◽  
Vol 72 (1) ◽  
Author(s):  
Sheng-Chao Huang ◽  
Yi-Fan Bao ◽  
Si-Si Wu ◽  
Teng-Xiang Huang ◽  
Matthew M. Sartin ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Surface Science ◽  
Spatial Resolution ◽  
Rational Design ◽  
Annual Review ◽  
Chemical Information ◽  
Publication Date ◽  
Molecular Fingerprint ◽  
Tip Enhanced Raman Spectroscopy

Revealing the intrinsic relationships between the structure, properties, and performance of the electrochemical interface is a long-term goal in the electrochemistry and surface science communities because they could facilitate the rational design of electrochemical devices. Achieving this goal requires in situ characterization techniques that provide rich chemical information and high spatial resolution. Electrochemical tip-enhanced Raman spectroscopy (EC-TERS), which provides molecular fingerprint information with nanometer-scale spatial resolution, is a promising technique for achieving this goal. Since the first demonstration of this technique in 2015, EC-TERS has been developed for characterizing various electrochemical processes at the nanoscale and molecular level. Here, we review the development of EC-TERS over the past 5 years. We discuss progress in addressing the technical challenges, including optimizing the EC-TERS setup and solving tip-related issues, and provide experimental guidelines. We also survey the important applications of EC-TERS for probing molecular protonation, molecular adsorption, electrochemical reactions, and photoelectrochemical reactions. Finally, we discuss the opportunities and challenges in the future development of this young technique. Expected final online publication date for the Annual Review of Physical Chemistry, Volume 72 is April 2021. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

In Situ Raman Spectroscopy Studies on La2CaB10O19 Crystal Growth

2020 ◽  
Vol 20 (10) ◽  
pp. 6604-6609
Author(s):  
Shanshan Liu ◽  
Guochun Zhang ◽  
Kai Feng ◽  
Yanyang Han ◽  
Tao He ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Crystal Growth ◽  
In Situ Raman Spectroscopy ◽  
In Situ Raman ◽  
Spectroscopy Studies

scholarly journals Retraction of “High-Capacitance Mechanism for Ti3C2Tx MXene by in Situ Electrochemical Raman Spectroscopy Investigation″

ACS Nano ◽  
2021 ◽  
Author(s):  
Minmin Hu ◽  
Zhaojin Li ◽  
Tao Hu ◽  
Shihao Zhu ◽  
Chao Zhang ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
High Capacitance

The use of in-situ Raman spectroscopy in investigating carbon materials as the anodes of alkali metal-ion batteries

Carbon ◽  
2021 ◽  
Vol 177 ◽  
pp. 428
Author(s):  
Xiaoqin Cheng ◽  
Huijun Li ◽  
Zhenxin Zhao ◽  
Yong-zhen Wang ◽  
Xiaomin Wang
Keyword(s):  
Raman Spectroscopy ◽  
Alkali Metal ◽  
Metal Ion ◽  
Carbon Materials ◽  
In Situ Raman Spectroscopy ◽  
Alkali Metal Ion ◽  
In Situ Raman

Calibration Methods to Circumvent Unknown Component Spectra for Quantitative in situ Raman Monitoring of Co-Polymerisation Reaction

2021 ◽  
Author(s):  
Wendy Rusli ◽  
Pavan Kumar Naraharisetti ◽  
Wee Chew
Keyword(s):  
Raman Spectroscopy ◽  
Reaction Monitoring ◽  
The Past ◽  
In Situ Raman ◽  
Calibration Methods ◽  
Unknown Component

The use of Raman spectroscopy for reaction monitoring has been successfully applied over the past few decades. One complication in such usage is the applicability for quantitative reaction studies. This...

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